Click here to switch to the map view.

The map label for this gene is gmd [H]

Identifier: 37678533

GI number: 37678533

Start: 357438

End: 358559

Strand: Direct

Name: gmd [H]

Synonym: VV0349

Alternate gene names: 37678533

Gene position: 357438-358559 (Clockwise)

Preceding gene: 37678532

Following gene: 37678534

Centisome position: 10.66

GC content: 41.71

Gene sequence:

>1122_bases
ATGCCAAATAAAGTTGCCTTAATTACAGGTGTAACGGGTCAGGACGGATCATACTTAGCAGAGTTCCTTTTAGAAAAAGG
TTATGAGGTTCATGGTATTAAGCGCCGCGCCTCAAGTTTTAATACAGAGCGTGTAGACCATATTTATCAAGATAGACACG
ACGATAACCCTAAATTTTTCCTTCATTATGGTGACCTCACAGATACTTCAAACTTAACTCGTATTCTTAAAGAGGTGCAA
CCTGATGAGGTGTATAACTTAGGAGCCCAAAGCCATGTCGCCGTATCGTTTGAAGCGCCAGAATACACAGCTGATGTAGA
TGCAATTGGTACGCTGCGTTTGCTTGAAGCAATCCGTTTCCTTGGATTAGAGAAGAAAACAAAATTCTACCAAGCATCAA
CGTCAGAGTTGTACGGTGAAGTGCAAGAGATTCCGCAAAAGGAAACTACTCCTTTCTACCCACGTTCACCATACGCTGTT
GCAAAAATGTATGCTTACTGGATTACAGTAAATTACCGTGAGTCTTATGGTATGTATGCATGTAACGGTATTCTGTTTAA
CCATGAATCACCTCGTCGCGGCGAAACGTTCGTAACGCGAAAAATAACACGTGGCCTTGCCAATATTTCTCAAGGTTTAG
AGAAGTGTTTATATCTAGGTAATATGGATGCTCTTCGCGATTGGGGGCACGCAAAAGACTATGTACGTATGCAGTGGATG
ATGCTTCAGCAAGTTACACCAGAAGACTTTGTTATTGCAACAGGAAGACAAATCTCTGTCAGAGAATTTGTATCTTTATC
GGCCAAAGAGCTAGGGATTACGCTGGAATTTAGCGGTGAAGGTATTGATGAAATAGCGACAGTTGTATCTATTGACGGTG
AAAATGCACAAGTACTTAAAGTAGGTGATGTTATTGTACGTGTAGACCCGCGTTATTTCCGTCCAGCCGAAGTTGAAACA
TTGTTAGGCGATCCAACCAAAGCCAAAGAAAAGTTAGGTTGGGAGCCAGAGATTACTGTTGAAGAAATGTGTGCAGAAAT
GGTTCAACATGACTTAGCTAAAGCGAAGCAGCATGCATTATTGAAATCTCACGGCTACTCTGTGAGTGTTCCAGTAGAGT
AA

Upstream 100 bases:

>100_bases
TGAGACAATCTGAAGTTTCAAAGGTTTTAGAAAACATCCCTAGTTGGGATCAATATACACGTTTGCTAATTAAAAGTAGC
ATTTAAAAAGAGAGAATAAA

Downstream 100 bases:

>100_bases
TCTTAGAGCAGTAGGTCAGAAATAAAAAATGATAACCCAGCTTATGCTGGATTTTATTATGGATAAAGAAATGAGTTCTA
AAACAGTTTTTGTCGCAGGC

Product: GDP-mannose-4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDRHDDNPKFFLHYGDLTDTSNLTRILKEVQ
PDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEKKTKFYQASTSELYGEVQEIPQKETTPFYPRSPYAV
AKMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRGLANISQGLEKCLYLGNMDALRDWGHAKDYVRMQWM
MLQQVTPEDFVIATGRQISVREFVSLSAKELGITLEFSGEGIDEIATVVSIDGENAQVLKVGDVIVRVDPRYFRPAEVET
LLGDPTKAKEKLGWEPEITVEEMCAEMVQHDLAKAKQHALLKSHGYSVSVPVE

Sequences:

>Translated_373_residues
MPNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDRHDDNPKFFLHYGDLTDTSNLTRILKEVQ
PDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEKKTKFYQASTSELYGEVQEIPQKETTPFYPRSPYAV
AKMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRGLANISQGLEKCLYLGNMDALRDWGHAKDYVRMQWM
MLQQVTPEDFVIATGRQISVREFVSLSAKELGITLEFSGEGIDEIATVVSIDGENAQVLKVGDVIVRVDPRYFRPAEVET
LLGDPTKAKEKLGWEPEITVEEMCAEMVQHDLAKAKQHALLKSHGYSVSVPVE
>Mature_372_residues
PNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDRHDDNPKFFLHYGDLTDTSNLTRILKEVQP
DEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEKKTKFYQASTSELYGEVQEIPQKETTPFYPRSPYAVA
KMYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRGLANISQGLEKCLYLGNMDALRDWGHAKDYVRMQWMM
LQQVTPEDFVIATGRQISVREFVSLSAKELGITLEFSGEGIDEIATVVSIDGENAQVLKVGDVIVRVDPRYFRPAEVETL
LGDPTKAKEKLGWEPEITVEEMCAEMVQHDLAKAKQHALLKSHGYSVSVPVE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=354, Percent_Identity=60.4519774011299, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI1788366, Length=371, Percent_Identity=82.4797843665768, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1788353, Length=377, Percent_Identity=24.9336870026525, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994969, Length=366, Percent_Identity=26.775956284153, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=352, Percent_Identity=63.6363636363636, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17539424, Length=359, Percent_Identity=61.0027855153203, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=359, Percent_Identity=61.0027855153203, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=359, Percent_Identity=61.0027855153203, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=359, Percent_Identity=61.0027855153203, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=359, Percent_Identity=61.0027855153203, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24158427, Length=359, Percent_Identity=57.3816155988858, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42240; Mature: 42109

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDRHDDNPKFF
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHCCCCCCCEEE
LHYGDLTDTSNLTRILKEVQPDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRF
EEECCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LGLEKKTKFYQASTSELYGEVQEIPQKETTPFYPRSPYAVAKMYAYWITVNYRESYGMYA
HCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCEEE
CNGILFNHESPRRGETFVTRKITRGLANISQGLEKCLYLGNMDALRDWGHAKDYVRMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
MLQQVTPEDFVIATGRQISVREFVSLSAKELGITLEFSGEGIDEIATVVSIDGENAQVLK
HHHHCCCCCEEEECCCEEEHHHHHHCCHHHCCEEEEECCCCHHHHEEEEEECCCCCEEEE
VGDVIVRVDPRYFRPAEVETLLGDPTKAKEKLGWEPEITVEEMCAEMVQHDLAKAKQHAL
ECCEEEEECCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKSHGYSVSVPVE
HHHCCCEEEECCC
>Mature Secondary Structure 
PNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDRHDDNPKFF
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHCCCCCCCEEE
LHYGDLTDTSNLTRILKEVQPDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRF
EEECCCCCHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LGLEKKTKFYQASTSELYGEVQEIPQKETTPFYPRSPYAVAKMYAYWITVNYRESYGMYA
HCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCEEE
CNGILFNHESPRRGETFVTRKITRGLANISQGLEKCLYLGNMDALRDWGHAKDYVRMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
MLQQVTPEDFVIATGRQISVREFVSLSAKELGITLEFSGEGIDEIATVVSIDGENAQVLK
HHHHCCCCCEEEECCCEEEHHHHHHCCHHHCCEEEEECCCCHHHHEEEEEECCCCCEEEE
VGDVIVRVDPRYFRPAEVETLLGDPTKAKEKLGWEPEITVEEMCAEMVQHDLAKAKQHAL
ECCEEEEECCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKSHGYSVSVPVE
HHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]