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The map label for this gene is hisF2

Identifier: 37678503

GI number: 37678503

Start: 327408

End: 328226

Strand: Direct

Name: hisF2

Synonym: VV0319

Alternate gene names: 37678503

Gene position: 327408-328226 (Clockwise)

Preceding gene: 37678502

Following gene: 37678504

Centisome position: 9.76

GC content: 39.07

Gene sequence:

>819_bases
GTGTTTCACATGCTTAAAATAAGATTAATTCCATGCATAGTCACCAAAGGTGAACTAGTAGTGCAGAGTTTTGCATTCAA
AAACTACTTACCAATAGGAAATGTAAAGACAGCGATTGATTTTTTTGTGAACTGGGATGTTGATGAAATCATTGTAAATG
ATATTGATGCATCGAAAGAGTTTAGAGAGCCAAATGTCGATCTTGTTAGCTGGGCAGCTAAAGAGTGTTTTGTACCGCTG
ACTGTTGGCGGTGGTATCAAGACTTTGGAGCATATAAGAAATCTACTCAAAGCTGGAGCGGATAAAGTTACCATAAATAC
TAAAGCTATTGATGACCCGGATTTTATTAAAAATGCAGCTTCAGTTTTTGGGAGCCAATGCATAACTGTTTCGGTTGATG
CAATTAAGCAGGGTAATGTTTATAAACTCTATGATTATAGAGATGGTCGGGTTTTAGATGTAGATGTTGTTGATTGGGTT
CGAAAAGTAGAGTCCTACGGTGCTGGAGAAATTCTACTGAATTCTGTAGATCGCGATGGCAGCAGAGAAGGCTATGACGT
AGAGTTATTGAAAACTGTAAGCGGTATTGTGTCCATTCCGGTGATTGCACTAGGAGGGATTGGAAGGTTTGACCAGTTAG
CTGAAGGAGCTATAGAAGGTGGATGCCAAGCCTTATCTGCGGCTAATATTTTTCAGCATATGGAACATAGTACTATTGCT
GCAAAAGCTCAAATGAGAAACGCTAAACTGAATGTACGACTTAGTTCCAAAGTTAAGTATGAGAACTTTGATCTAGATTT
TCTTGGCCGCCCGTATTGA

Upstream 100 bases:

>100_bases
GAAAATATCGTTGGAATGCAATTTCATCCAGAGAAAAGTCAGAGGAATGGCCTTGAAGCAATTCGAAGCTTTCTAGATTG
GGTAAAAATTCAAAAATTAG

Downstream 100 bases:

>100_bases
TTGTAAATAATTAAGAATTTGAACTATGAAATATTGTAAGCGTTGTTTATACCCAGAGAATCATGCACTAAATCTCATTT
TTGATGTGGATGGTGTATGT

Product: imidazoleglycerol-phosphate synthase

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MFHMLKIRLIPCIVTKGELVVQSFAFKNYLPIGNVKTAIDFFVNWDVDEIIVNDIDASKEFREPNVDLVSWAAKECFVPL
TVGGGIKTLEHIRNLLKAGADKVTINTKAIDDPDFIKNAASVFGSQCITVSVDAIKQGNVYKLYDYRDGRVLDVDVVDWV
RKVESYGAGEILLNSVDRDGSREGYDVELLKTVSGIVSIPVIALGGIGRFDQLAEGAIEGGCQALSAANIFQHMEHSTIA
AKAQMRNAKLNVRLSSKVKYENFDLDFLGRPY

Sequences:

>Translated_272_residues
MFHMLKIRLIPCIVTKGELVVQSFAFKNYLPIGNVKTAIDFFVNWDVDEIIVNDIDASKEFREPNVDLVSWAAKECFVPL
TVGGGIKTLEHIRNLLKAGADKVTINTKAIDDPDFIKNAASVFGSQCITVSVDAIKQGNVYKLYDYRDGRVLDVDVVDWV
RKVESYGAGEILLNSVDRDGSREGYDVELLKTVSGIVSIPVIALGGIGRFDQLAEGAIEGGCQALSAANIFQHMEHSTIA
AKAQMRNAKLNVRLSSKVKYENFDLDFLGRPY
>Mature_272_residues
MFHMLKIRLIPCIVTKGELVVQSFAFKNYLPIGNVKTAIDFFVNWDVDEIIVNDIDASKEFREPNVDLVSWAAKECFVPL
TVGGGIKTLEHIRNLLKAGADKVTINTKAIDDPDFIKNAASVFGSQCITVSVDAIKQGNVYKLYDYRDGRVLDVDVVDWV
RKVESYGAGEILLNSVDRDGSREGYDVELLKTVSGIVSIPVIALGGIGRFDQLAEGAIEGGCQALSAANIFQHMEHSTIA
AKAQMRNAKLNVRLSSKVKYENFDLDFLGRPY

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=33.852140077821, Blast_Score=146, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=239, Percent_Identity=31.7991631799163, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS62_VIBVY (Q7MPP3)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_933112.1
- ProteinModelPortal:   Q7MPP3
- SMR:   Q7MPP3
- STRING:   Q7MPP3
- GeneID:   2623086
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0319
- NMPDR:   fig|196600.1.peg.387
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ANASACA
- ProtClustDB:   CLSK2522163
- BioCyc:   VVUL196600:VV0319-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR03572

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 30050; Mature: 30050

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHMLKIRLIPCIVTKGELVVQSFAFKNYLPIGNVKTAIDFFVNWDVDEIIVNDIDASKE
CCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCHHHHHHCCCCCCHH
FREPNVDLVSWAAKECFVPLTVGGGIKTLEHIRNLLKAGADKVTINTKAIDDPDFIKNAA
HCCCCCHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
SVFGSQCITVSVDAIKQGNVYKLYDYRDGRVLDVDVVDWVRKVESYGAGEILLNSVDRDG
HHHCCCEEEEEEECCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCC
SREGYDVELLKTVSGIVSIPVIALGGIGRFDQLAEGAIEGGCQALSAANIFQHMEHSTIA
CCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAQMRNAKLNVRLSSKVKYENFDLDFLGRPY
HHHHHCCCEEEEEEECEEEEECCCCCCCCCCC
>Mature Secondary Structure
MFHMLKIRLIPCIVTKGELVVQSFAFKNYLPIGNVKTAIDFFVNWDVDEIIVNDIDASKE
CCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCHHHHHHCCCCCCHH
FREPNVDLVSWAAKECFVPLTVGGGIKTLEHIRNLLKAGADKVTINTKAIDDPDFIKNAA
HCCCCCHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
SVFGSQCITVSVDAIKQGNVYKLYDYRDGRVLDVDVVDWVRKVESYGAGEILLNSVDRDG
HHHCCCEEEEEEECCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCC
SREGYDVELLKTVSGIVSIPVIALGGIGRFDQLAEGAIEGGCQALSAANIFQHMEHSTIA
CCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAQMRNAKLNVRLSSKVKYENFDLDFLGRPY
HHHHHCCCEEEEEEECEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA