Click here to switch to the map view.

The map label for this gene is hisH2

Identifier: 37678502

GI number: 37678502

Start: 326780

End: 327424

Strand: Direct

Name: hisH2

Synonym: VV0318

Alternate gene names: 37678502

Gene position: 326780-327424 (Clockwise)

Preceding gene: 37678501

Following gene: 37678503

Centisome position: 9.74

GC content: 36.28

Gene sequence:

>645_bases
ATGAAAATAGTAATTATTGATTATGATATGGGTAATGTTCGTTCAATAGAAAATGCTATTAACCATATTGGAGACTACAC
AATTATAGTATCAGGAGAGCCAGATACGATCCGCTCTGCAGATTGCCTTATCCTACCGGGTGTTGGAGCATTTCCTGACG
CAATGAAAAAGCTTGAACAACAGAAATTGATAGATTTGCTTACTGAAGAGGTCGTAGTTAGGAGGAAGCCTGTTTTAGGG
ATCTGTTTAGGTATGCAGTTGCTCTTTGAATCCTCTGAGGAGATTCAGCTTACAAAAGGTTTAGGTTGGATTCCTGGTAA
AGTAGAATATATGAGGCCCGGGAATGACTTAAGAGTACCACATGTTGGTTGGAACTCACTGATATTAAAAAAAGAAAATA
GCTTGTTCGATTATCTTCAAGACGACAAAGATTTTTATTTTGTACATAGTTTATGGGTAAATTGCCCAGAAAAATATAAG
TTGGCTACATTTGAATATGGCATTGAAATGACAGCCTCAGTTCAATATGAAAATATCGTTGGAATGCAATTTCATCCAGA
GAAAAGTCAGAGGAATGGCCTTGAAGCAATTCGAAGCTTTCTAGATTGGGTAAAAATTCAAAAATTAGGTGTTTCACATG
CTTAA

Upstream 100 bases:

>100_bases
GTTGGTTCTGTACTGTTTTTGCTTTCAGAGCAGTCACGTTATGTAACAGGACAGAACATTGTTGTAGATGATGGTTTTAG
TTTGTAATTTGGAATTGTAA

Downstream 100 bases:

>100_bases
AATAAGATTAATTCCATGCATAGTCACCAAAGGTGAACTAGTAGTGCAGAGTTTTGCATTCAAAAACTACTTACCAATAG
GAAATGTAAAGACAGCGATT

Product: glutamine amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKIVIIDYDMGNVRSIENAINHIGDYTIIVSGEPDTIRSADCLILPGVGAFPDAMKKLEQQKLIDLLTEEVVVRRKPVLG
ICLGMQLLFESSEEIQLTKGLGWIPGKVEYMRPGNDLRVPHVGWNSLILKKENSLFDYLQDDKDFYFVHSLWVNCPEKYK
LATFEYGIEMTASVQYENIVGMQFHPEKSQRNGLEAIRSFLDWVKIQKLGVSHA

Sequences:

>Translated_214_residues
MKIVIIDYDMGNVRSIENAINHIGDYTIIVSGEPDTIRSADCLILPGVGAFPDAMKKLEQQKLIDLLTEEVVVRRKPVLG
ICLGMQLLFESSEEIQLTKGLGWIPGKVEYMRPGNDLRVPHVGWNSLILKKENSLFDYLQDDKDFYFVHSLWVNCPEKYK
LATFEYGIEMTASVQYENIVGMQFHPEKSQRNGLEAIRSFLDWVKIQKLGVSHA
>Mature_214_residues
MKIVIIDYDMGNVRSIENAINHIGDYTIIVSGEPDTIRSADCLILPGVGAFPDAMKKLEQQKLIDLLTEEVVVRRKPVLG
ICLGMQLLFESSEEIQLTKGLGWIPGKVEYMRPGNDLRVPHVGWNSLILKKENSLFDYLQDDKDFYFVHSLWVNCPEKYK
LATFEYGIEMTASVQYENIVGMQFHPEKSQRNGLEAIRSFLDWVKIQKLGVSHA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=40.0990099009901, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=36.5384615384615, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS52_VIBVY (Q7MPP4)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_933111.1
- ProteinModelPortal:   Q7MPP4
- SMR:   Q7MPP4
- STRING:   Q7MPP4
- GeneID:   2623085
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0318
- NMPDR:   fig|196600.1.peg.386
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- BioCyc:   VVUL196600:VV0318-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 24406; Mature: 24406

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 185-185 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIIDYDMGNVRSIENAINHIGDYTIIVSGEPDTIRSADCLILPGVGAFPDAMKKLEQ
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHH
QKLIDLLTEEVVVRRKPVLGICLGMQLLFESSEEIQLTKGLGWIPGKVEYMRPGNDLRVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCEECC
HVGWNSLILKKENSLFDYLQDDKDFYFVHSLWVNCPEKYKLATFEYGIEMTASVQYENIV
CCCCCEEEEECCCCHHHHHCCCCCEEEEEEHHHCCCCHHEEEEEEECEEEEEEEEECEEE
GMQFHPEKSQRNGLEAIRSFLDWVKIQKLGVSHA
CEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIVIIDYDMGNVRSIENAINHIGDYTIIVSGEPDTIRSADCLILPGVGAFPDAMKKLEQ
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHH
QKLIDLLTEEVVVRRKPVLGICLGMQLLFESSEEIQLTKGLGWIPGKVEYMRPGNDLRVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCEECC
HVGWNSLILKKENSLFDYLQDDKDFYFVHSLWVNCPEKYKLATFEYGIEMTASVQYENIV
CCCCCEEEEECCCCHHHHHCCCCCEEEEEEHHHCCCCHHEEEEEEECEEEEEEEEECEEE
GMQFHPEKSQRNGLEAIRSFLDWVKIQKLGVSHA
CEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA