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The map label for this gene is hsdR [H]

Identifier: 37678449

GI number: 37678449

Start: 274695

End: 277148

Strand: Direct

Name: hsdR [H]

Synonym: VV0265

Alternate gene names: 37678449

Gene position: 274695-277148 (Clockwise)

Preceding gene: 37678448

Following gene: 37678450

Centisome position: 8.19

GC content: 45.76

Gene sequence:

>2454_bases
ATGAGGTGGATCAGTGGGTTAGATCTGGTGGCGCATCAGATGACAGCAAGGAAGAAAAATAGTATGGCCTTAAACGAAGC
AGATACTTGCCGGGTCTATGTGACGCCAAAGCTCAAAGAGTCTGGATGGGAAAACAATCCTAGCGCAATAACTGAACAGT
ACACATTTACCGATGGTCGTGTTCAGTTTAAGGGCAGTAAAGTTCAACGTGGGGAGCAAAAGCGAGCGGATTATTTACTT
AAGTACACTCGCGATTTTCCTATTGCCGTTGTTGAGGCTAAACCTGAAAACAGCCCTGTTGGCCAAGGGATGCAACAGGC
AAAAGATTACGCAGAAATCTTAGGGCTAAAGTTTGCTTACTCAACCAATGGTCATGAGATCTTAGAATTTGACTACACCA
CTGGTGAAGAGCAATTGTTGTCGCGCTTTCCAACCCCTGATGAGTTATTCAAGCGTCTTTGTGGCGATGAAGGTATAAAG
GATGAAGATCTTGATACCTTGCTGTCCCCTTACCACCATGTTTCGGGATATTCCCCGCGCTACTACCAACAAATAGCTAT
AAACCGCGCAGTTCAGTCGGTGCTTCAAGGTAAAAAGCGCTCATTAATTACGATGGCAACGGGTACGGGTAAGACTGTCG
TCGCTTTTCAAATTAGTTGGAAGCTGTGGAGTGCTCGCTGGAATCGTACGGGAGACTACAGAAAGCCTCGCATTCTATTT
TTGGCAGATAGAAATGTGTTGGTCGATGACCCAAAGGATAAAACATTTACTCCGTTTGGTGATGCAAGGCACAAAATTGA
AGGTGGCAAAGTTGTTAAAAGTCGTGAAATTTATTTTGCCATTTATCAGTCCATTGCCAGTGATGAGCGTCGTCCAGGCT
TGTATAAAGAATTTCCGCAAGACTTCTTTGATCTAATTATCATTGATGAATGTCACCGAGGCAGTGCCCGCGACAACAGT
AACTGGCGCGAAATTCTGGAGTATTTTGAGCCAGCCTTTCAAATCGGTATGACGGCGACGCCGCTTCGTGAAGATAACCG
CGATACTTATCGTTATTTTGGCAACCCAATCTACACCTACAGTTTGCGCCAAGGTATTGATGACGGATTTCTTGCTCCAT
ACCGCGTACACCGAGTTATTTCGGAAGTCGATGCGGCAGGTTGGCGACCAAGCAAAGGCGATGTAGACCGCTTTGGACGA
GAAATCCCAGATGGTGAATACCAAACCAAGGACTTTGAACGGGTTATTGCGTTAAAGGCTCGAACAGATGCGTTTGCTAA
GCATCTCACCGACTTTATGAAGCGAACCGATCGTTTTGCAAAAACGATCGTGTTTTGTGTCGACCAAGAACACGCCGATG
AAATGCGTCGGGCGCTTAATAACTTGAATTCTGACCTTTCACGCAAGCATCCAGATTACGTTGCACGCGTGACATCAGAA
GAAGGGAAAATTGGCAAAGGGCACCTCAGTCGTTTTCAAGAGTTGGAAACCAGTACTCCAGTGATACTGACCACTTCACA
GTTATTAACAACGGGGGTCGATGCGCCAACCTGTAAAAATGTTGTGCTTGCGCGTGTTGTGAACTCAATGAGTGAGTTCA
AGCAAATTGTTGGCCGTGGTACTCGCCTACGCGAAGATTACGGCAAGCTGTGGTTCAACATCATTGATTATACCGGCTCC
GCCACACAAAATTTTGCTGATCCTGACTTTGACGGTTACCCAGAAATTGAAGATGAAGTGGTGATTGATGAAGACGGTGA
AGAAGTGGTTGATGACACTTTAACACCAGAGAGTGAAGTGTTAGAGGTTGCTGAAGAGACGGGCGAATACGAAGTGAATA
CTGAAGGTGAAGGCGCTGATGATGAAGAGGAGCACGAACCGAGAAAGTATTACGTAGACGGTGGCAGCGTTAAAATTACG
TCTCATTTGGTCTATGAGCTAGACGAAGACGGTAAACAGCTTCGCGTCATCCAATATACCGACTATACCGCAGAAAAGGT
AAAGACCCTCTTTTCCTCACTGGATGAACTAAAAGATAGCTGGGCTAATCCGTTAAAGAGAGAAGAGATCATTCTCGAAT
TACAAGAACGAGGGATCCAGTTTGAAGATTTGGTCGCAGAGTCGGGTAAAACCGATGCAGATCCATTGGATTTACTTTGT
CACATAGCATTTAACGTACCGCTGCGAACCAGAAAAGAGCGCGCTGACTATTTGAAGAAAAACAAACCGGACTTTTTTGA
TCAGTACGGCCCAGAGGCACGCGCGATCTTAACGTCTTTACTGGACAAATACACCGACCACGGCCCTAAGCAATTTAGTA
TTCCGGATTCGCTACAGGTACCGCCCATTTCTGAATATGGCAATGTGATGGAAATAGCCAACCTCTTTGGCGGCGCATTG
CAAATGAAGACCGCCGTGGACCAATTACAAACGCTGCTCTATAGCCAGCAATAA

Upstream 100 bases:

>100_bases
GCCGAATATCTTGGCGTAAGCAAAGATACTGTGTACTCATGGATCAGTAAAAAGGGTATGCCAGCTCATAGAATTGGTCG
GCCTTGGAAGTTTAAAGCAG

Downstream 100 bases:

>100_bases
CAGAAGTGACGATACGATTTATGGCACGAACAAAGAAAGCAGATCAGCCGATGACCACCGCCCAGCAATTAGGTGCGATT
GTTAAATCGTCTCGGCAAAT

Product: type I site-specific restriction-modification system, R (restriction) subunit

Products: NA

Alternate protein names: R.EcoAI [H]

Number of amino acids: Translated: 817; Mature: 817

Protein sequence:

>817_residues
MRWISGLDLVAHQMTARKKNSMALNEADTCRVYVTPKLKESGWENNPSAITEQYTFTDGRVQFKGSKVQRGEQKRADYLL
KYTRDFPIAVVEAKPENSPVGQGMQQAKDYAEILGLKFAYSTNGHEILEFDYTTGEEQLLSRFPTPDELFKRLCGDEGIK
DEDLDTLLSPYHHVSGYSPRYYQQIAINRAVQSVLQGKKRSLITMATGTGKTVVAFQISWKLWSARWNRTGDYRKPRILF
LADRNVLVDDPKDKTFTPFGDARHKIEGGKVVKSREIYFAIYQSIASDERRPGLYKEFPQDFFDLIIIDECHRGSARDNS
NWREILEYFEPAFQIGMTATPLREDNRDTYRYFGNPIYTYSLRQGIDDGFLAPYRVHRVISEVDAAGWRPSKGDVDRFGR
EIPDGEYQTKDFERVIALKARTDAFAKHLTDFMKRTDRFAKTIVFCVDQEHADEMRRALNNLNSDLSRKHPDYVARVTSE
EGKIGKGHLSRFQELETSTPVILTTSQLLTTGVDAPTCKNVVLARVVNSMSEFKQIVGRGTRLREDYGKLWFNIIDYTGS
ATQNFADPDFDGYPEIEDEVVIDEDGEEVVDDTLTPESEVLEVAEETGEYEVNTEGEGADDEEEHEPRKYYVDGGSVKIT
SHLVYELDEDGKQLRVIQYTDYTAEKVKTLFSSLDELKDSWANPLKREEIILELQERGIQFEDLVAESGKTDADPLDLLC
HIAFNVPLRTRKERADYLKKNKPDFFDQYGPEARAILTSLLDKYTDHGPKQFSIPDSLQVPPISEYGNVMEIANLFGGAL
QMKTAVDQLQTLLYSQQ

Sequences:

>Translated_817_residues
MRWISGLDLVAHQMTARKKNSMALNEADTCRVYVTPKLKESGWENNPSAITEQYTFTDGRVQFKGSKVQRGEQKRADYLL
KYTRDFPIAVVEAKPENSPVGQGMQQAKDYAEILGLKFAYSTNGHEILEFDYTTGEEQLLSRFPTPDELFKRLCGDEGIK
DEDLDTLLSPYHHVSGYSPRYYQQIAINRAVQSVLQGKKRSLITMATGTGKTVVAFQISWKLWSARWNRTGDYRKPRILF
LADRNVLVDDPKDKTFTPFGDARHKIEGGKVVKSREIYFAIYQSIASDERRPGLYKEFPQDFFDLIIIDECHRGSARDNS
NWREILEYFEPAFQIGMTATPLREDNRDTYRYFGNPIYTYSLRQGIDDGFLAPYRVHRVISEVDAAGWRPSKGDVDRFGR
EIPDGEYQTKDFERVIALKARTDAFAKHLTDFMKRTDRFAKTIVFCVDQEHADEMRRALNNLNSDLSRKHPDYVARVTSE
EGKIGKGHLSRFQELETSTPVILTTSQLLTTGVDAPTCKNVVLARVVNSMSEFKQIVGRGTRLREDYGKLWFNIIDYTGS
ATQNFADPDFDGYPEIEDEVVIDEDGEEVVDDTLTPESEVLEVAEETGEYEVNTEGEGADDEEEHEPRKYYVDGGSVKIT
SHLVYELDEDGKQLRVIQYTDYTAEKVKTLFSSLDELKDSWANPLKREEIILELQERGIQFEDLVAESGKTDADPLDLLC
HIAFNVPLRTRKERADYLKKNKPDFFDQYGPEARAILTSLLDKYTDHGPKQFSIPDSLQVPPISEYGNVMEIANLFGGAL
QMKTAVDQLQTLLYSQQ
>Mature_817_residues
MRWISGLDLVAHQMTARKKNSMALNEADTCRVYVTPKLKESGWENNPSAITEQYTFTDGRVQFKGSKVQRGEQKRADYLL
KYTRDFPIAVVEAKPENSPVGQGMQQAKDYAEILGLKFAYSTNGHEILEFDYTTGEEQLLSRFPTPDELFKRLCGDEGIK
DEDLDTLLSPYHHVSGYSPRYYQQIAINRAVQSVLQGKKRSLITMATGTGKTVVAFQISWKLWSARWNRTGDYRKPRILF
LADRNVLVDDPKDKTFTPFGDARHKIEGGKVVKSREIYFAIYQSIASDERRPGLYKEFPQDFFDLIIIDECHRGSARDNS
NWREILEYFEPAFQIGMTATPLREDNRDTYRYFGNPIYTYSLRQGIDDGFLAPYRVHRVISEVDAAGWRPSKGDVDRFGR
EIPDGEYQTKDFERVIALKARTDAFAKHLTDFMKRTDRFAKTIVFCVDQEHADEMRRALNNLNSDLSRKHPDYVARVTSE
EGKIGKGHLSRFQELETSTPVILTTSQLLTTGVDAPTCKNVVLARVVNSMSEFKQIVGRGTRLREDYGKLWFNIIDYTGS
ATQNFADPDFDGYPEIEDEVVIDEDGEEVVDDTLTPESEVLEVAEETGEYEVNTEGEGADDEEEHEPRKYYVDGGSVKIT
SHLVYELDEDGKQLRVIQYTDYTAEKVKTLFSSLDELKDSWANPLKREEIILELQERGIQFEDLVAESGKTDADPLDLLC
HIAFNVPLRTRKERADYLKKNKPDFFDQYGPEARAILTSLLDKYTDHGPKQFSIPDSLQVPPISEYGNVMEIANLFGGAL
QMKTAVDQLQTLLYSQQ

Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=649, Percent_Identity=26.6563944530046, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320539, Length=393, Percent_Identity=28.2442748091603, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 93437; Mature: 93437

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWISGLDLVAHQMTARKKNSMALNEADTCRVYVTPKLKESGWENNPSAITEQYTFTDGR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCCCCCCHHEEEEEECCCE
VQFKGSKVQRGEQKRADYLLKYTRDFPIAVVEAKPENSPVGQGMQQAKDYAEILGLKFAY
EEECCCHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEE
STNGHEILEFDYTTGEEQLLSRFPTPDELFKRLCGDEGIKDEDLDTLLSPYHHVSGYSPR
CCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCH
YYQQIAINRAVQSVLQGKKRSLITMATGTGKTVVAFQISWKLWSARWNRTGDYRKPRILF
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEEEHHHCCCCCCCCCCEEEE
LADRNVLVDDPKDKTFTPFGDARHKIEGGKVVKSREIYFAIYQSIASDERRPGLYKEFPQ
EECCCEEEECCCCCCCCCCCCCHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHH
DFFDLIIIDECHRGSARDNSNWREILEYFEPAFQIGMTATPLREDNRDTYRYFGNPIYTY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEE
SLRQGIDDGFLAPYRVHRVISEVDAAGWRPSKGDVDRFGREIPDGEYQTKDFERVIALKA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
RTDAFAKHLTDFMKRTDRFAKTIVFCVDQEHADEMRRALNNLNSDLSRKHPDYVARVTSE
HHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCHHHHHCCCCHHEEEECC
EGKIGKGHLSRFQELETSTPVILTTSQLLTTGVDAPTCKNVVLARVVNSMSEFKQIVGRG
CCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TRLREDYGKLWFNIIDYTGSATQNFADPDFDGYPEIEDEVVIDEDGEEVVDDTLTPESEV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCHHHH
LEVAEETGEYEVNTEGEGADDEEEHEPRKYYVDGGSVKITSHLVYELDEDGKQLRVIQYT
HHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEEEECCCCCEEEEEEEC
DYTAEKVKTLFSSLDELKDSWANPLKREEIILELQERGIQFEDLVAESGKTDADPLDLLC
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHH
HIAFNVPLRTRKERADYLKKNKPDFFDQYGPEARAILTSLLDKYTDHGPKQFSIPDSLQV
HHHHCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCC
PPISEYGNVMEIANLFGGALQMKTAVDQLQTLLYSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRWISGLDLVAHQMTARKKNSMALNEADTCRVYVTPKLKESGWENNPSAITEQYTFTDGR
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCCCCCCHHEEEEEECCCE
VQFKGSKVQRGEQKRADYLLKYTRDFPIAVVEAKPENSPVGQGMQQAKDYAEILGLKFAY
EEECCCHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEE
STNGHEILEFDYTTGEEQLLSRFPTPDELFKRLCGDEGIKDEDLDTLLSPYHHVSGYSPR
CCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCH
YYQQIAINRAVQSVLQGKKRSLITMATGTGKTVVAFQISWKLWSARWNRTGDYRKPRILF
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEEEHHHCCCCCCCCCCEEEE
LADRNVLVDDPKDKTFTPFGDARHKIEGGKVVKSREIYFAIYQSIASDERRPGLYKEFPQ
EECCCEEEECCCCCCCCCCCCCHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHH
DFFDLIIIDECHRGSARDNSNWREILEYFEPAFQIGMTATPLREDNRDTYRYFGNPIYTY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEE
SLRQGIDDGFLAPYRVHRVISEVDAAGWRPSKGDVDRFGREIPDGEYQTKDFERVIALKA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
RTDAFAKHLTDFMKRTDRFAKTIVFCVDQEHADEMRRALNNLNSDLSRKHPDYVARVTSE
HHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCHHHHHCCCCHHEEEECC
EGKIGKGHLSRFQELETSTPVILTTSQLLTTGVDAPTCKNVVLARVVNSMSEFKQIVGRG
CCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TRLREDYGKLWFNIIDYTGSATQNFADPDFDGYPEIEDEVVIDEDGEEVVDDTLTPESEV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCHHHH
LEVAEETGEYEVNTEGEGADDEEEHEPRKYYVDGGSVKITSHLVYELDEDGKQLRVIQYT
HHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEEEECCCCCEEEEEEEC
DYTAEKVKTLFSSLDELKDSWANPLKREEIILELQERGIQFEDLVAESGKTDADPLDLLC
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHH
HIAFNVPLRTRKERADYLKKNKPDFFDQYGPEARAILTSLLDKYTDHGPKQFSIPDSLQV
HHHHCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCC
PPISEYGNVMEIANLFGGALQMKTAVDQLQTLLYSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]