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The map label for this gene is hsdM [H]

Identifier: 37678450

GI number: 37678450

Start: 277154

End: 278746

Strand: Direct

Name: hsdM [H]

Synonym: VV0266

Alternate gene names: 37678450

Gene position: 277154-278746 (Clockwise)

Preceding gene: 37678449

Following gene: 37678451

Centisome position: 8.26

GC content: 45.57

Gene sequence:

>1593_bases
GTGACGATACGATTTATGGCACGAACAAAGAAAGCAGATCAGCCGATGACCACCGCCCAGCAATTAGGTGCGATTGTTAA
ATCGTCTCGGCAAATCATGCGTAAAGATAAAGGCTTAAATGGCGATCTCGATCGCCTGCCAATGCTCACTTGGATCATGT
TTTTGAAGTTTCTCGACGACTTAGAACAAATGCGAGAAACCGAAGCCGTTCTAGAAGGAAAAAGCTTTCAGCCAGCCATC
GAAGCGCCATACCGCTGGCGAGATTGGGCAGCAATTGAAGGTGGTATCACCGGCGACGAGTTAATTGCCTTTATTAACAA
CGACGAAGCAATGCGACCTGATGGTACCCGTGGCATCGGTTTGTTTGCGTACTTGCGTAGCCTGCAAGGTGACAACGGTG
GTGATCGCCGAGATGTGATTGCGACCGTGTTTAAAGGTATGCAAAACCGCATGATCAACGGGTATTTGTTACGTGATGTC
GTCGACAAAATAAACGGCATTCATTTCAATTCATCTGAAGAGATGCACACCTTAAGCCGCTTATACGAAACCATGCTGCG
TGAAATGCGTGATGCCGCTGGTGACTCCGGCGAGTTTTATACTCCGCGTCCAGTGGTTCGCTTTATGGTTGAGGTGATGG
ACCCGCAGTTGGGTGAGTCCGTATTGGACCCAGCCTGCGGCACGGGTGGTTTTTTGGTTGAAGCGTTCGAACACCTAGAG
CGCCAGTGTAAAACAGTCGAAGACCGCGAAGTATTGCAAGAAAGCAGTATCTTTGGCGGAGAAGCCAAATCGTTGCCTTA
CCTGTTGGTACAAATGAACCTGTTACTGCATGGTTTGGAGTACCCAAGAATTGACCCAGAAAATAGCCTGCGTTTTCCAC
TACGAGAGATGGGCGATAAAGATCGTGTAGATGTGATCTTAACCAACCCACCGTTTGGTGGTGAGGAAGAAAAAGGCATT
CTTGGTAACTTCCCGGAAGATATGCAAACCGCCGAAACCGCAATGCTGTTCCTTCAATTGATCATGCGTAAACTCAAACG
CCCTGGTCATGGTTCAGACAATGGTGGCCGCGCCGCAGTTGTTGTACCAAATGGCACGTTATTCAGTGATGGTATCAGCG
CACGCATTAAAGAAGAGCTGTTAAAGAACTTTAATCTGCATACCATTGTTCGTTTGCCGGAAGGGGTATTCGCGCCTTAT
ACCGATATTGCAGGCAACTTACTCTTCTTCGATCGTTCTGGTCCTACAGACGATATTTGGTATTACCAAATCACTGTTCC
TGAAGGTCGTAAAAAATATACTAAAACTAAACCAATGGAAAGCCATGAGTTTGATGAATGCTTGAACTGGTGGAGTAACA
GGATCGTTAATCAGAATGCCTGGAAAGAAAGTGCCTCAGAGATTATTAAATACTCCGAAAGTGGACAACTGATTGACGTA
AATTTAGATAGAAAAAATCCTAATAGTCTCGAAGTGCTTGAACACGAAGAGCCTATGGATTTGATAGAGAAAATAACCAC
GAAAGAAGAGTCTCTTTTAGCGATCTTAGAACAGATTAAAGCCGAAATAAGCACTCAAGGTATCTCTAAATGA

Upstream 100 bases:

>100_bases
GCAATGTGATGGAAATAGCCAACCTCTTTGGCGGCGCATTGCAAATGAAGACCGCCGTGGACCAATTACAAACGCTGCTC
TATAGCCAGCAATAACAGAA

Downstream 100 bases:

>100_bases
AAGAGTATACTTTAAGGGATGTTCTTATCCGCCAAAAAGAGGCCATCACCGTTGAGGATGATGCTGAGTATAAGCGCATT
ACCATTAAAATGAATGGTAA

Product: type I restriction-modification system methyltransferase subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTIRFMARTKKADQPMTTAQQLGAIVKSSRQIMRKDKGLNGDLDRLPMLTWIMFLKFLDDLEQMRETEAVLEGKSFQPAI
EAPYRWRDWAAIEGGITGDELIAFINNDEAMRPDGTRGIGLFAYLRSLQGDNGGDRRDVIATVFKGMQNRMINGYLLRDV
VDKINGIHFNSSEEMHTLSRLYETMLREMRDAAGDSGEFYTPRPVVRFMVEVMDPQLGESVLDPACGTGGFLVEAFEHLE
RQCKTVEDREVLQESSIFGGEAKSLPYLLVQMNLLLHGLEYPRIDPENSLRFPLREMGDKDRVDVILTNPPFGGEEEKGI
LGNFPEDMQTAETAMLFLQLIMRKLKRPGHGSDNGGRAAVVVPNGTLFSDGISARIKEELLKNFNLHTIVRLPEGVFAPY
TDIAGNLLFFDRSGPTDDIWYYQITVPEGRKKYTKTKPMESHEFDECLNWWSNRIVNQNAWKESASEIIKYSESGQLIDV
NLDRKNPNSLEVLEHEEPMDLIEKITTKEESLLAILEQIKAEISTQGISK

Sequences:

>Translated_530_residues
MTIRFMARTKKADQPMTTAQQLGAIVKSSRQIMRKDKGLNGDLDRLPMLTWIMFLKFLDDLEQMRETEAVLEGKSFQPAI
EAPYRWRDWAAIEGGITGDELIAFINNDEAMRPDGTRGIGLFAYLRSLQGDNGGDRRDVIATVFKGMQNRMINGYLLRDV
VDKINGIHFNSSEEMHTLSRLYETMLREMRDAAGDSGEFYTPRPVVRFMVEVMDPQLGESVLDPACGTGGFLVEAFEHLE
RQCKTVEDREVLQESSIFGGEAKSLPYLLVQMNLLLHGLEYPRIDPENSLRFPLREMGDKDRVDVILTNPPFGGEEEKGI
LGNFPEDMQTAETAMLFLQLIMRKLKRPGHGSDNGGRAAVVVPNGTLFSDGISARIKEELLKNFNLHTIVRLPEGVFAPY
TDIAGNLLFFDRSGPTDDIWYYQITVPEGRKKYTKTKPMESHEFDECLNWWSNRIVNQNAWKESASEIIKYSESGQLIDV
NLDRKNPNSLEVLEHEEPMDLIEKITTKEESLLAILEQIKAEISTQGISK
>Mature_529_residues
TIRFMARTKKADQPMTTAQQLGAIVKSSRQIMRKDKGLNGDLDRLPMLTWIMFLKFLDDLEQMRETEAVLEGKSFQPAIE
APYRWRDWAAIEGGITGDELIAFINNDEAMRPDGTRGIGLFAYLRSLQGDNGGDRRDVIATVFKGMQNRMINGYLLRDVV
DKINGIHFNSSEEMHTLSRLYETMLREMRDAAGDSGEFYTPRPVVRFMVEVMDPQLGESVLDPACGTGGFLVEAFEHLER
QCKTVEDREVLQESSIFGGEAKSLPYLLVQMNLLLHGLEYPRIDPENSLRFPLREMGDKDRVDVILTNPPFGGEEEKGIL
GNFPEDMQTAETAMLFLQLIMRKLKRPGHGSDNGGRAAVVVPNGTLFSDGISARIKEELLKNFNLHTIVRLPEGVFAPYT
DIAGNLLFFDRSGPTDDIWYYQITVPEGRKKYTKTKPMESHEFDECLNWWSNRIVNQNAWKESASEIIKYSESGQLIDVN
LDRKNPNSLEVLEHEEPMDLIEKITTKEESLLAILEQIKAEISTQGISK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=284, Percent_Identity=30.6338028169014, Blast_Score=128, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 60156; Mature: 60025

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRFMARTKKADQPMTTAQQLGAIVKSSRQIMRKDKGLNGDLDRLPMLTWIMFLKFLDD
CEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LEQMRETEAVLEGKSFQPAIEAPYRWRDWAAIEGGITGDELIAFINNDEAMRPDGTRGIG
HHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHCCCCCCHHHEEEEECCCCCCCCCCCCCHH
LFAYLRSLQGDNGGDRRDVIATVFKGMQNRMINGYLLRDVVDKINGIHFNSSEEMHTLSR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LYETMLREMRDAAGDSGEFYTPRPVVRFMVEVMDPQLGESVLDPACGTGGFLVEAFEHLE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHH
RQCKTVEDREVLQESSIFGGEAKSLPYLLVQMNLLLHGLEYPRIDPENSLRFPLREMGDK
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCC
DRVDVILTNPPFGGEEEKGILGNFPEDMQTAETAMLFLQLIMRKLKRPGHGSDNGGRAAV
CCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VVPNGTLFSDGISARIKEELLKNFNLHTIVRLPEGVFAPYTDIAGNLLFFDRSGPTDDIW
EECCCCEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCEE
YYQITVPEGRKKYTKTKPMESHEFDECLNWWSNRIVNQNAWKESASEIIKYSESGQLIDV
EEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
NLDRKNPNSLEVLEHEEPMDLIEKITTKEESLLAILEQIKAEISTQGISK
EECCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIRFMARTKKADQPMTTAQQLGAIVKSSRQIMRKDKGLNGDLDRLPMLTWIMFLKFLDD
EEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LEQMRETEAVLEGKSFQPAIEAPYRWRDWAAIEGGITGDELIAFINNDEAMRPDGTRGIG
HHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHCCCCCCHHHEEEEECCCCCCCCCCCCCHH
LFAYLRSLQGDNGGDRRDVIATVFKGMQNRMINGYLLRDVVDKINGIHFNSSEEMHTLSR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LYETMLREMRDAAGDSGEFYTPRPVVRFMVEVMDPQLGESVLDPACGTGGFLVEAFEHLE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHH
RQCKTVEDREVLQESSIFGGEAKSLPYLLVQMNLLLHGLEYPRIDPENSLRFPLREMGDK
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCC
DRVDVILTNPPFGGEEEKGILGNFPEDMQTAETAMLFLQLIMRKLKRPGHGSDNGGRAAV
CCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VVPNGTLFSDGISARIKEELLKNFNLHTIVRLPEGVFAPYTDIAGNLLFFDRSGPTDDIW
EECCCCEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCEEEEECCCCCCCEE
YYQITVPEGRKKYTKTKPMESHEFDECLNWWSNRIVNQNAWKESASEIIKYSESGQLIDV
EEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
NLDRKNPNSLEVLEHEEPMDLIEKITTKEESLLAILEQIKAEISTQGISK
EECCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]