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The map label for this gene is epsK [H]

Identifier: 37678405

GI number: 37678405

Start: 232066

End: 233106

Strand: Direct

Name: epsK [H]

Synonym: VV0221

Alternate gene names: 37678405

Gene position: 232066-233106 (Clockwise)

Preceding gene: 37678404

Following gene: 37678406

Centisome position: 6.92

GC content: 50.53

Gene sequence:

>1041_bases
GTGGTGGAGCAGGCAACAATGGCTAAATCGAACCGCTCGCGCCAATCTGGAGTGGCCCTGATCATCATTTTGCTCTTGCT
GGCCATTATGGCCACCATCGCAGGCAGTATGTCTGAGCGTTTGTTTGCACAGTTTAAGCGTGGCACCAACCAGATCAGCT
ATCAGCAAGCTTACTGGTACAGCATTGGCGTAGAAGCGTTGGCGAAGGTCGGTATTGAGCAAAGTTACAAAGACAGTGAC
ACCATTAACTTAAACCAGCCTTGGGCCTTGGAAGAGAAAACCTATCCCTTGGACTATGGCCAAGCGAAAGGACGCATTTT
TGATAAGCAAGCGTGCTTTAATCTCAATGCTCTCTCTCCCGTGGTGATGCAAGACGCAGCGGTAGAGCGTCCTTATTTGG
TGAAAGTCTTTCGGGCTTTGCTCGAAGAGTCAGGCGTTGAAAGTTATGAATCCGAAGTGATTGCTGACTCCATGTGGGAG
TTTGTCGATCCCAATGACGCGGTGCGCTCTTCTTCTGGGGTGGAAGACTCGACCTATGAAGGGATGAAACCCGCCTATTT
GGCGGCGAATGGGTACATTGCTGACAGCAGTGAACTGCGCGCGGTTTACCAAGTGTCGGGTGAAATGATGGCGACGCTGC
AGCCTTTGGTCTGTGCTTTACCGACCGATGAGTGGCGCTTGAACGTCAATACCATCAGCGCTGAGCAAGCTGCTTTGCTG
GTGGCGCTGTTTTCGCCCGCGTTGAGTGAATCTGATGCCAAGCAACTGATAGAAAAACGCCCCTTTGATGGCTGGGCGAA
CATTGATGCTTTCCTTTCAGAATCTGAAATGGCGGGCATCAGCGAAGAAATCAAAAAACTAGCAAAAGCGTATCTTGGCG
TTGATAGCCGATACTTTGAATTGGATGCACAGGTGTTAGTCGAGGACTCTCGGGTGAGAATTCGCAGCCTTATGTACAGT
AAAAACAGAGAAACCGTGACCGTTGTGCGTCGCCGTTTTGGAGGAATCAGTGAGCGAGTTTCTGACCGTTCGGCTGAGTA
G

Upstream 100 bases:

>100_bases
ACGTTGAAAGATTACGGGAAGATCGCGCGAACCTACCTCACTCCAGAGGGGAATTTGCAAAAACAAGCGTCAGGTAGCGA
AGACTCCGGGCAAAACAATA

Downstream 100 bases:

>100_bases
CGAACAATATAGCCCCATTCCTTGGTTGGTTTGGTCATCAAGCCAACAAGAAGTGATCGCCAGTGGGGAGTTGTCGGATT
GGCAACAACTGGACGATCTC

Product: type II secretory pathway, component EpsK

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MVEQATMAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSD
TINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWE
FVDPNDAVRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALL
VALFSPALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYS
KNRETVTVVRRRFGGISERVSDRSAE

Sequences:

>Translated_346_residues
MVEQATMAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSD
TINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWE
FVDPNDAVRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALL
VALFSPALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYS
KNRETVTVVRRRFGGISERVSDRSAE
>Mature_346_residues
MVEQATMAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSD
TINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWE
FVDPNDAVRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALL
VALFSPALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYS
KNRETVTVVRRRFGGISERVSDRSAE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=300, Percent_Identity=30, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 38553; Mature: 38553

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQATMAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWY
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SIGVEALAKVGIEQSYKDSDTINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSP
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCH
VVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPNDAVRSSSGVEDSTYE
HHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
GMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALL
CCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHH
VALFSPALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFE
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
LDAQVLVEDSRVRIRSLMYSKNRETVTVVRRRFGGISERVSDRSAE
ECHHHEECHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MVEQATMAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWY
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SIGVEALAKVGIEQSYKDSDTINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSP
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCH
VVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPNDAVRSSSGVEDSTYE
HHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
GMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALL
CCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHH
VALFSPALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFE
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
LDAQVLVEDSRVRIRSLMYSKNRETVTVVRRRFGGISERVSDRSAE
ECHHHEECHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]