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The map label for this gene is epsJ [H]

Identifier: 37678404

GI number: 37678404

Start: 231390

End: 232091

Strand: Direct

Name: epsJ [H]

Synonym: VV0220

Alternate gene names: 37678404

Gene position: 231390-232091 (Clockwise)

Preceding gene: 37678403

Following gene: 37678405

Centisome position: 6.9

GC content: 51.71

Gene sequence:

>702_bases
ATGTGGCGAAATAAAACGTGTCGAAAGAGAAACACGCGCCAACGTGGTTTTACCCTCATCGAAGTGTTGGTGTCGATTGC
CATTTTTGCCACCTTAAGTGTGGCCGCATATCAGGTGGTCAATCAGGTGCAACGCAGCAATGAGTTATCACAAGAGCGCA
CCGCACGATTGAATGAATTGCAACGTGCCTTGGTGATGATGGACAGCGATTTTCGCCAGATAGCGCTGCGTCAAACCCGC
ACCAACGGCGAAGAGCCGAGCAAAAAACTGCTGCATTGGGCGGATTACCTCTTAGATTCAGACAGCAAAGGCATCATGTT
CGCGCGTTTAGGTTGGCACAATCCGCAGCAGCAATTCCCTCGCGGGGAGGTCACCAAAGTGGGTTATCGCATCAAAGATG
AGCGCTTAGAGCGAGTATGGTGGCGTTACCCAGATACCCCAGCAGGGCAAGAAGGGGTCGTGACTCCGTTACTCAGTGAT
GTGGAAGAGCTTAATGTGCGTTTTTATGACGGCAAACAGTGGATCAATGAGTGGAGCAACGAGTTGGCCTTGCCGGCGGC
GATCTCGGTAGAGCTGACGTTGAAAGATTACGGGAAGATCGCGCGAACCTACCTCACTCCAGAGGGGAATTTGCAAAAAC
AAGCGTCAGGTAGCGAAGACTCCGGGCAAAACAATAGTGGTGGAGCAGGCAACAATGGCTAA

Upstream 100 bases:

>100_bases
AAAGTCGCACCCGTAGCCACGACCCAGCCGCTGCTGAAAGCGTTTGATGTGAGTGTGGCAGCCGAAAAGCAGGCCAGCCC
AATAATTACGGTGAGAAGCT

Downstream 100 bases:

>100_bases
ATCGAACCGCTCGCGCCAATCTGGAGTGGCCCTGATCATCATTTTGCTCTTGCTGGCCATTATGGCCACCATCGCAGGCA
GTATGTCTGAGCGTTTGTTT

Product: type II secretory pathway, component EpsJ

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTARLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWINEWSNELALPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG

Sequences:

>Translated_233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTARLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWINEWSNELALPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
>Mature_233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTARLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWINEWSNELALPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

Organism=Escherichia coli, GI1789728, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 26618; Mature: 26618

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTARLNEL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALVMMDSDFRQIALRQTRTNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFP
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCC
RGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSDVEELNVRFYDGKQWINEWSN
CCCHHHHCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEEECCHHHHHHHHH
ELALPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
CCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTARLNEL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALVMMDSDFRQIALRQTRTNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFP
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCC
RGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSDVEELNVRFYDGKQWINEWSN
CCCHHHHCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEEECCHHHHHHHHH
ELALPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
CCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]