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The map label for this gene is gldA

Identifier: 37678342

GI number: 37678342

Start: 163739

End: 164827

Strand: Direct

Name: gldA

Synonym: VV0158

Alternate gene names: 37678342

Gene position: 163739-164827 (Clockwise)

Preceding gene: 37678340

Following gene: 37678343

Centisome position: 4.88

GC content: 42.15

Gene sequence:

>1089_bases
ATGTTAACAACAACTATATTTCCAGGTCGTTATGTTCAAGGTAAAGATGCTCGAAAACAACTTGGAGTAGAACTCAATCG
TTTTGGAAAAAAAGGTCTAATGATCTGTGACTCATTTGTTTATGATACATTATTTTCTGAGTTTGAACCTTATCTTGAGA
CCGTTGAAGTTGAAGTGATCCGGTTTGCTAGTGAATGTTGTGACGAAGAAATAACACGTCTGACAGCCATGTTGAAAAAT
TTTGAGGGTGAATTTGTGGTTGGATTAGGTGGAGGAAAAACCATTGATACAGCAAAAGCAACCTCGTATGAAGCGAGAGT
CCCTGTGGCAATTGTGCCGACTTTGGCAGCGAGCGATGCACCATGCAGTGCACTATCCGTTATTTACACTCAAGAAGGAG
AGTTTAAGCGTTATCTTTTCCTGCCTCAAAATCCGAATCTTGTCTTGGTCGACACACAAATTATTGCCAAGGCACCTGTT
CGATTCCTTATAGCTGGGATCGGTGATGCGCTAGCAACATGGTTTGAAGCAGAATCATGCCAGCAAAGTTTTGCCTCGAA
TATGACAGGTTATATGGGTTCATTGTCAGCATATGCGTTAGCACGTTTGTGTTACGACACTTTGCTTGAATACGGCGTAA
TAGCTAAAACATCGAATGAACAAGGTGTGGTTACTCCAGCGCTCGAACGTGTTGTGGAAGCAAATACGTTGCTTAGTGGA
TTAGGTTTCGAAAGTGGTGGTCTTGCTGCTTCTCATGCGATTCATAATGGTTTAACAGTATTGGAGCCAACACATGTGTT
TTTGCATGGTGAAAAAGTCGCATTTGGTTTGCTTTCTTCTCTATTCCTTACTGATAAACCAGCACAGACCATCGCAACTG
TTTATGACTTCTGCGAAAAAGTTGGCTTGCCAACGACTCTGTCTCAAATCGGTTTAGAAAATGTATCCGATGAAGATTTA
TTGGCAGTGGCTGAAGCTTCATGTGCAGAAGGTGAGACCATCCACAACGAACCTCGAACTGTAACTCCAGATATGGTGTT
TGCAGCAATGAAAGCGGCAAATGTAGAAGGGTTACGTCGCAAGAGCTAA

Upstream 100 bases:

>100_bases
ACTATGACTTCTTATTTGAGAGTTAAAAACTACCATTTTAACACGAATTATCGATGTCAGAACCACGGTAAAAAATTAAA
TCAGTTATCTAGGAGTTATT

Downstream 100 bases:

>100_bases
CACAGCTTATCATTACATTCATCCCCGCTTTTGTCTGCAAATTAATTGGTAGACTCTATGAGGTGGTTTATTCCTGAATT
AGCTTCTATATGTCAAGAGA

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MLTTTIFPGRYVQGKDARKQLGVELNRFGKKGLMICDSFVYDTLFSEFEPYLETVEVEVIRFASECCDEEITRLTAMLKN
FEGEFVVGLGGGKTIDTAKATSYEARVPVAIVPTLAASDAPCSALSVIYTQEGEFKRYLFLPQNPNLVLVDTQIIAKAPV
RFLIAGIGDALATWFEAESCQQSFASNMTGYMGSLSAYALARLCYDTLLEYGVIAKTSNEQGVVTPALERVVEANTLLSG
LGFESGGLAASHAIHNGLTVLEPTHVFLHGEKVAFGLLSSLFLTDKPAQTIATVYDFCEKVGLPTTLSQIGLENVSDEDL
LAVAEASCAEGETIHNEPRTVTPDMVFAAMKAANVEGLRRKS

Sequences:

>Translated_362_residues
MLTTTIFPGRYVQGKDARKQLGVELNRFGKKGLMICDSFVYDTLFSEFEPYLETVEVEVIRFASECCDEEITRLTAMLKN
FEGEFVVGLGGGKTIDTAKATSYEARVPVAIVPTLAASDAPCSALSVIYTQEGEFKRYLFLPQNPNLVLVDTQIIAKAPV
RFLIAGIGDALATWFEAESCQQSFASNMTGYMGSLSAYALARLCYDTLLEYGVIAKTSNEQGVVTPALERVVEANTLLSG
LGFESGGLAASHAIHNGLTVLEPTHVFLHGEKVAFGLLSSLFLTDKPAQTIATVYDFCEKVGLPTTLSQIGLENVSDEDL
LAVAEASCAEGETIHNEPRTVTPDMVFAAMKAANVEGLRRKS
>Mature_362_residues
MLTTTIFPGRYVQGKDARKQLGVELNRFGKKGLMICDSFVYDTLFSEFEPYLETVEVEVIRFASECCDEEITRLTAMLKN
FEGEFVVGLGGGKTIDTAKATSYEARVPVAIVPTLAASDAPCSALSVIYTQEGEFKRYLFLPQNPNLVLVDTQIIAKAPV
RFLIAGIGDALATWFEAESCQQSFASNMTGYMGSLSAYALARLCYDTLLEYGVIAKTSNEQGVVTPALERVVEANTLLSG
LGFESGGLAASHAIHNGLTVLEPTHVFLHGEKVAFGLLSSLFLTDKPAQTIATVYDFCEKVGLPTTLSQIGLENVSDEDL
LAVAEASCAEGETIHNEPRTVTPDMVFAAMKAANVEGLRRKS

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=354, Percent_Identity=53.1073446327684, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786815, Length=299, Percent_Identity=34.4481605351171, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39145; Mature: 39145

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTTIFPGRYVQGKDARKQLGVELNRFGKKGLMICDSFVYDTLFSEFEPYLETVEVEVI
CCEEEECCCCCCCCCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFASECCDEEITRLTAMLKNFEGEFVVGLGGGKTIDTAKATSYEARVPVAIVPTLAASDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCCCEEEECCCCCCCC
PCSALSVIYTQEGEFKRYLFLPQNPNLVLVDTQIIAKAPVRFLIAGIGDALATWFEAESC
CCCCEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSFASNMTGYMGSLSAYALARLCYDTLLEYGVIAKTSNEQGVVTPALERVVEANTLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
LGFESGGLAASHAIHNGLTVLEPTHVFLHGEKVAFGLLSSLFLTDKPAQTIATVYDFCEK
CCCCCCCCHHHHHHHCCCEEECCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGLPTTLSQIGLENVSDEDLLAVAEASCAEGETIHNEPRTVTPDMVFAAMKAANVEGLRR
CCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCC
KS
CC
>Mature Secondary Structure
MLTTTIFPGRYVQGKDARKQLGVELNRFGKKGLMICDSFVYDTLFSEFEPYLETVEVEVI
CCEEEECCCCCCCCCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFASECCDEEITRLTAMLKNFEGEFVVGLGGGKTIDTAKATSYEARVPVAIVPTLAASDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCCCEEEECCCCCCCC
PCSALSVIYTQEGEFKRYLFLPQNPNLVLVDTQIIAKAPVRFLIAGIGDALATWFEAESC
CCCCEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSFASNMTGYMGSLSAYALARLCYDTLLEYGVIAKTSNEQGVVTPALERVVEANTLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
LGFESGGLAASHAIHNGLTVLEPTHVFLHGEKVAFGLLSSLFLTDKPAQTIATVYDFCEK
CCCCCCCCHHHHHHHCCCEEECCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGLPTTLSQIGLENVSDEDLLAVAEASCAEGETIHNEPRTVTPDMVFAAMKAANVEGLRR
CCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12193646 [H]