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The map label for this gene is rpoH

Identifier: 37678299

GI number: 37678299

Start: 123253

End: 124110

Strand: Direct

Name: rpoH

Synonym: VV0115

Alternate gene names: 37678299

Gene position: 123253-124110 (Clockwise)

Preceding gene: 37678298

Following gene: 161486673

Centisome position: 3.67

GC content: 49.07

Gene sequence:

>858_bases
ATGACAACTCAAGCGTATCCGATGGCACTAGTAACGCAAGATAGCCTTGATGGCTACATTCGCTCGGTGAACAGCTACCC
AATGCTGACCGCAGACGAAGAACGTGATCTAGCTGAACGTCTACACTACAAAGGCGAAATTGAAGCGGCAAAAGGGCTGA
TTCTCTCTCACCTGAGATTCGTTGTTCACGTTGCCCGCGGTTATTCTGGCTACGGTTTACCAATGGCTGATTTAGTACAA
GAAGGCAATATTGGTCTCATGAAGGCGGTAAAACGCTTCAATCCAGAAGTTGGGGTGAGGCTAGTTTCTTTTGCGGTTCA
CTGGATCAAAGCGGAAATTCACGAGTACGTACTACGTAACTGGCGTATTGTGAAGATCGCGACCACCAAAGCGCAGCGTA
AGTTGTTCTTCAACCTACGTAAGTCGAAAAAACGTCTTGGTTGGTTTAACAATGGCGAAGTCGAAACCGTGGCTCGTGAG
TTGGGCGTAGAACCGGCAGAAGTGCGAGAAATGGAATCGCGTTTGGCGGCGGTCGATTCGACCTTTGATTTGCCGAATGA
AGAAGACGATTCTAGCTCGGTATCAACGGCACCAGTCCTTTATCTGGAAGACAAATCTTCAGATGTGGCGGACAACCTAG
AAGCGCAAAACTGGGAAGCTCACACCAATAACCGCCTTGCGATGGCATTGGCTAGCCTTGATGAGCGTAGCCAACATATT
GTTCGCTCGCGTTGGTTGGACGATGATAAAGCCACGCTGCAAGATCTCGCGGAAATGTATGGTGTTTCTGCGGAGCGAAT
CCGTCAGCTTGAAAAGAACGCGATGAAGAAACTGAAGATGGCCGTCGGCGAGATTTAG

Upstream 100 bases:

>100_bases
ATAATTGCTAACCCCGCTTGAAACTGATTCATCTTCAGTTCAAGATAACGGCGCTAATTTGCAATGTATAACTCCAGATC
AGAGATTGATGAGGAATTGA

Downstream 100 bases:

>100_bases
TTCTCTTACGAAACCACTTAAGAATCAGCAAAAGCCGAGATCAATGATCTCGGCTTTTTTGTTGCCTGTCACAACATCAT
GGCGAGTGTTTTTGATCGAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTTQAYPMALVTQDSLDGYIRSVNSYPMLTADEERDLAERLHYKGEIEAAKGLILSHLRFVVHVARGYSGYGLPMADLVQ
EGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARE
LGVEPAEVREMESRLAAVDSTFDLPNEEDDSSSVSTAPVLYLEDKSSDVADNLEAQNWEAHTNNRLAMALASLDERSQHI
VRSRWLDDDKATLQDLAEMYGVSAERIRQLEKNAMKKLKMAVGEI

Sequences:

>Translated_285_residues
MTTQAYPMALVTQDSLDGYIRSVNSYPMLTADEERDLAERLHYKGEIEAAKGLILSHLRFVVHVARGYSGYGLPMADLVQ
EGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARE
LGVEPAEVREMESRLAAVDSTFDLPNEEDDSSSVSTAPVLYLEDKSSDVADNLEAQNWEAHTNNRLAMALASLDERSQHI
VRSRWLDDDKATLQDLAEMYGVSAERIRQLEKNAMKKLKMAVGEI
>Mature_284_residues
TTQAYPMALVTQDSLDGYIRSVNSYPMLTADEERDLAERLHYKGEIEAAKGLILSHLRFVVHVARGYSGYGLPMADLVQE
GNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVAREL
GVEPAEVREMESRLAAVDSTFDLPNEEDDSSSVSTAPVLYLEDKSSDVADNLEAQNWEAHTNNRLAMALASLDERSQHIV
RSRWLDDDKATLQDLAEMYGVSAERIRQLEKNAMKKLKMAVGEI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=71.3780918727915, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=26.6423357664234, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=27.5193798449612, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RP32_VIBVU (Q8DD54)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760103.1
- ProteinModelPortal:   Q8DD54
- SMR:   Q8DD54
- GeneID:   1178085
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1158
- HOGENOM:   HBG745096
- OMA:   VNSIPVL
- ProtClustDB:   PRK06596
- BioCyc:   VVUL216895:VV1_1158-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02392
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 32326; Mature: 32195

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAYPMALVTQDSLDGYIRSVNSYPMLTADEERDLAERLHYKGEIEAAKGLILSHLRF
CCCCCCCEEEEEHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELGVEPAEVREMESRLAAVDS
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDLPNEEDDSSSVSTAPVLYLEDKSSDVADNLEAQNWEAHTNNRLAMALASLDERSQHI
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VRSRWLDDDKATLQDLAEMYGVSAERIRQLEKNAMKKLKMAVGEI
HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAYPMALVTQDSLDGYIRSVNSYPMLTADEERDLAERLHYKGEIEAAKGLILSHLRF
CCCCCCEEEEEHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELGVEPAEVREMESRLAAVDS
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDLPNEEDDSSSVSTAPVLYLEDKSSDVADNLEAQNWEAHTNNRLAMALASLDERSQHI
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VRSRWLDDDKATLQDLAEMYGVSAERIRQLEKNAMKKLKMAVGEI
HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA