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The map label for this gene is glpE

Identifier: 161486673

GI number: 161486673

Start: 124546

End: 124866

Strand: Direct

Name: glpE

Synonym: VV0117

Alternate gene names: 161486673

Gene position: 124546-124866 (Clockwise)

Preceding gene: 37678299

Following gene: 37678302

Centisome position: 3.71

GC content: 50.47

Gene sequence:

>321_bases
ATGGAACAGTTCAAGCATATTGATGTACAAGGCGCGCACGCGCTCATCAGTCGTGGAGAAGCACGCTTGGTGGATATTCG
TGACCCACAATCTTTTGCTGTGGCGCATGCCCAATCGGCCTTTCATCTAACCAACGATTCGATCGTAAATTTCATGCAGC
AGGTGGAGTTTGAACAGCCTGTTTTGGTGATGTGTTACCACGGCATCAGCAGTCAAGGGGCGGCCCAGTACCTGGTCAAT
CAAGGTTTTGAAGAGGTGTACAGTGTCGATGGTGGTTTTGAAGCTTGGCATCGTGCCAGTTTGCCCGTTGAAGCAAGCTG
A

Upstream 100 bases:

>100_bases
AAATGTACGCTTGTCAAAGACCAGAAGAACTGTGTGTCTAAGTTTGGCTCTGGATAGGTTACAATCCCAGCAGAACCAAA
TAACGAAAATGAGAAAGTAT

Downstream 100 bases:

>100_bases
TCCCTTTAGAAAGAAGGGCTTTTGCCTAACGGCCTTTGATGTGGAAGAATGAGGTTCGATTTTTCGTCTAACTGAGGTTT
CTCTATGCAGCGTCTTATTG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MEQFKHIDVQGAHALISRGEARLVDIRDPQSFAVAHAQSAFHLTNDSIVNFMQQVEFEQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRASLPVEAS

Sequences:

>Translated_106_residues
MEQFKHIDVQGAHALISRGEARLVDIRDPQSFAVAHAQSAFHLTNDSIVNFMQQVEFEQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRASLPVEAS
>Mature_106_residues
MEQFKHIDVQGAHALISRGEARLVDIRDPQSFAVAHAQSAFHLTNDSIVNFMQQVEFEQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRASLPVEAS

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1789831, Length=106, Percent_Identity=59.4339622641509, Blast_Score=133, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPE_VIBVU (Q8DD53)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760104.1
- ProteinModelPortal:   Q8DD53
- SMR:   Q8DD53
- GeneID:   1178086
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1160
- HOGENOM:   HBG743107
- OMA:   FSAWHEA
- ProtClustDB:   PRK00162
- BioCyc:   VVUL216895:VV1_1160-MONOMER
- BRENDA:   2.8.1.1
- GO:   GO:0005737
- HAMAP:   MF_01009
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 11792; Mature: 11792

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFKHIDVQGAHALISRGEARLVDIRDPQSFAVAHAQSAFHLTNDSIVNFMQQVEFEQP
CCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHCCCEECCCHHHHHHHHHHCCCCC
VLVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEAWHRASLPVEAS
EEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MEQFKHIDVQGAHALISRGEARLVDIRDPQSFAVAHAQSAFHLTNDSIVNFMQQVEFEQP
CCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHCCCEECCCHHHHHHHHHHCCCCC
VLVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEAWHRASLPVEAS
EEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA