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The map label for this gene is leuO

Identifier: 161486664

GI number: 161486664

Start: 477420

End: 478358

Strand: Direct

Name: leuO

Synonym: VV0493

Alternate gene names: 161486664

Gene position: 477420-478358 (Clockwise)

Preceding gene: 37678676

Following gene: 37678678

Centisome position: 14.23

GC content: 48.03

Gene sequence:

>939_bases
ATGAGCGCGATTGCCAGCTATCGAATGGAAAGTACTTTACGCGGAGTGGATCTCAACCTACTGACGGTATTTGATGCCGT
TATGCAAGAGCAAAATATTACTCGCGCAGCACATAATTTAGGCATGTCACAGCCTGCAGTGAGCAACGCGGTTGCTCGCT
TAAAAGTGATGTTTAATGATGAGCTTTTCATGCGTCAAGGGCGTGGTATTCAACCGACCCAGCGTGCTCGCCAATTATTT
GGCCCAGTCCGCCAAGCGCTGCAATTGATCCGCAATGAATTACCAAGTTCTGTATTCCAGCCTGAGTCTTCAACTCGTCT
GTTCAAACTGGCGATTTGCAGCCCATGTGATATGCGTTTTGCACCACGCATTATGGCAGACATTCATAACAAAGCGCCTA
ATGTACAATTGCATTTGGATGCAGAGTTTGATCGCATGCTCTCTGAGCGTATGCGCTATCAAGAAATCGACTTTGTGATT
GATTACGCACGTTTTGACGAGCAAGGCTTCTCAAGCACAGAGATCTTCAAAGATGAACTCGTGGTTGTGACGTCTAAGCA
GCATCCTCGTATTCAAGGTAAAGTGACAGAAGCGCAGTTGATGCAAGAGCGTCACGCGAAACTGTCTCGCATTCACGGCC
AACGCAGCTTCTCTGAGCAAGCGTATCGCGATCTGGATGTGGCGGCGTATTTCGAAGGCACAAGCTTGAGCAACGTTCTG
TATGTGGTTGGTCAATCTGAGCTTGTGACGATCGCTCCTCGCTGGATTGTGGAAAATGCGGCAAACAACGCGCAGCTACA
GATTCTTGACTTCCCATTTGAAAATCGTGAAATCAGCGGTTTCTTAAGCTGGCACGAGTCATCAGAAAAAGACAAAGGCC
ACATTTGGCTGCGTGATCTGCTGATGGTGACATGTGGCGAAGTGGTTGCTGCGGTATAA

Upstream 100 bases:

>100_bases
ATAAATATAAGAAATACAGGTTATCTATTCCTGACTATCAAATTCAAATTTGTTGACCGTTACACGAGAGGCGCACTAGA
TGTTAGATAAAAAAGATGCG

Downstream 100 bases:

>100_bases
CCGACACGGCCTGTTACAAATTGGATGCCCTCATTGAGCCTTGGCCATGAGGGCATTTTTTTATCTTGTTATTGATGATT
CTCTCTAATTGAGCATAAGT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPTQRARQLF
GPVRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPRIMADIHNKAPNVQLHLDAEFDRMLSERMRYQEIDFVI
DYARFDEQGFSSTEIFKDELVVVTSKQHPRIQGKVTEAQLMQERHAKLSRIHGQRSFSEQAYRDLDVAAYFEGTSLSNVL
YVVGQSELVTIAPRWIVENAANNAQLQILDFPFENREISGFLSWHESSEKDKGHIWLRDLLMVTCGEVVAAV

Sequences:

>Translated_312_residues
MSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPTQRARQLF
GPVRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPRIMADIHNKAPNVQLHLDAEFDRMLSERMRYQEIDFVI
DYARFDEQGFSSTEIFKDELVVVTSKQHPRIQGKVTEAQLMQERHAKLSRIHGQRSFSEQAYRDLDVAAYFEGTSLSNVL
YVVGQSELVTIAPRWIVENAANNAQLQILDFPFENREISGFLSWHESSEKDKGHIWLRDLLMVTCGEVVAAV
>Mature_311_residues
SAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQPTQRARQLFG
PVRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRFAPRIMADIHNKAPNVQLHLDAEFDRMLSERMRYQEIDFVID
YARFDEQGFSSTEIFKDELVVVTSKQHPRIQGKVTEAQLMQERHAKLSRIHGQRSFSEQAYRDLDVAAYFEGTSLSNVLY
VVGQSELVTIAPRWIVENAANNAQLQILDFPFENREISGFLSWHESSEKDKGHIWLRDLLMVTCGEVVAAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=295, Percent_Identity=52.5423728813559, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87081766, Length=290, Percent_Identity=24.4827586206897, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786820, Length=288, Percent_Identity=23.2638888888889, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789204, Length=219, Percent_Identity=26.9406392694064, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35597; Mature: 35466

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFND
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECC
ELFMRQGRGIQPTQRARQLFGPVRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCH
APRIMADIHNKAPNVQLHLDAEFDRMLSERMRYQEIDFVIDYARFDEQGFSSTEIFKDEL
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCE
VVVTSKQHPRIQGKVTEAQLMQERHAKLSRIHGQRSFSEQAYRDLDVAAYFEGTSLSNVL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEE
YVVGQSELVTIAPRWIVENAANNAQLQILDFPFENREISGFLSWHESSEKDKGHIWLRDL
EEECCCCEEEECHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEECCCCCCCCCHHHHHHH
LMVTCGEVVAAV
HHHHHHHHHHCC
>Mature Secondary Structure 
SAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFND
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECC
ELFMRQGRGIQPTQRARQLFGPVRQALQLIRNELPSSVFQPESSTRLFKLAICSPCDMRF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCCCH
APRIMADIHNKAPNVQLHLDAEFDRMLSERMRYQEIDFVIDYARFDEQGFSSTEIFKDEL
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCE
VVVTSKQHPRIQGKVTEAQLMQERHAKLSRIHGQRSFSEQAYRDLDVAAYFEGTSLSNVL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEE
YVVGQSELVTIAPRWIVENAANNAQLQILDFPFENREISGFLSWHESSEKDKGHIWLRDL
EEECCCCEEEECHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEECCCCCCCCCHHHHHHH
LMVTCGEVVAAV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]