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The map label for this gene is proB

Identifier: 161486649

GI number: 161486649

Start: 863123

End: 864262

Strand: Direct

Name: proB

Synonym: VV0858

Alternate gene names: 161486649

Gene position: 863123-864262 (Clockwise)

Preceding gene: 37679041

Following gene: 37679043

Centisome position: 25.73

GC content: 49.3

Gene sequence:

>1140_bases
ATGACAACGAATCAACAAAGCGTCGCTGCTTCTCAACCAAAAACTATCGTTGTGAAGCTTGGAACCAGTGTGTTAACAGG
TGGTACATTAGCGTTAGATCGCGCTCATATGGTTGAGCTTGCGCGCCAATGTGCAGAGTTAAAAAAACAAGGCCACTCTG
TGGTGGTCGTCTCGTCAGGCGCGATTGCCGCTGGACGTGAGCACTTAGGTTACCCCGCGCTGCCCAATGCGATGGCAAGC
AAACAGTTGCTTGCTGCGGTTGGGCAAAGTCAGTTGATTCAAACCTGGGAATCGTTATTTGCACTGTATGGCATCAAAAT
TGGCCAAATGTTGCTCACTCGAGCGGATCTCGAAGATCGCGAACGTTTTCTCAATGCTCGAGATACGATTAACGCGTTAG
TGGATAACGGCATTATCCCTGTCGTTAATGAAAATGACGCCGTCGCCACGAGCGAAATCAAAGTGGGTGACAACGACAAC
CTCTCTGCGTTGGTGGGCATTTTATGTGGTGCTGATAAACTACTGTTGCTGACAGACCAAAAAGGCTTGTATACCGCCGA
TCCGCGTAAAGATCCCAATGCTGAGCTGATCAAAGAAGTTAAAGTCATTGACGATACTTTGCGTAAAATTGCTGGTGGCA
GCGGTACAACATTAGGTACTGGTGGGATGGCAACAAAGCTCCAAGCTGCGGACATTGCGCGTCGTGCTGGCATTGAAGTC
ATCATTGCGGCAGGCCGTGGTCAAAATGTCATTTTTGATGCGTTGAGCCCAGCCCCTCAAGGAACCCGTTTCTTGCCTTG
TGAAGAGGCGTTGGAAAACCGCAAGCGTTGGATTCTCGCTGGCCCTGCGGCCTCTGGTGATATTGTGATTGACCAAGGTG
CGGTGAAAGCCGTGGTCGAAAAAGGGAGCAGTTTGTTGGCGAAAGGGGTGACCAAAGTACTTGGCGAGTTTTCTCGTGGC
GAAGTAGTTCGTGTAACGGATGCACAGGGCCATTTGGTTGCGCGAGGTATTGCGAGTTATTCCAACCAAGATATGGCGAA
AATTGCCGGTAAGCACAGCAAAGACATTATTTCGATTCTGGGCTATGACTATGGTTCAGAAGTGATTCACCGTGACGACA
TGGTTGTCATTCAAGAATAG

Upstream 100 bases:

>100_bases
AATCAAAAAGGTGCGATTTTCGCACCTTTTCTGCTTGTGAATTCTTCATTTGATTGTTAAAACATCTTCTCTATTTTTGT
GTTTCATTTCAGAAGACATC

Downstream 100 bases:

>100_bases
TCGCTCACCAGAATCAAAGAGGACAACAAAGTGGATTTAATCACATTAGGTAAAGCGGCAAAAGATGCGGCTTTTCAGCT
TGCCACCGCTTCTACGGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSGAIAAGREHLGYPALPNAMAS
KQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDRERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDN
LSALVGILCGADKLLLLTDQKGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVEKGSSLLAKGVTKVLGEFSRG
EVVRVTDAQGHLVARGIASYSNQDMAKIAGKHSKDIISILGYDYGSEVIHRDDMVVIQE

Sequences:

>Translated_379_residues
MTTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSGAIAAGREHLGYPALPNAMAS
KQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDRERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDN
LSALVGILCGADKLLLLTDQKGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVEKGSSLLAKGVTKVLGEFSRG
EVVRVTDAQGHLVARGIASYSNQDMAKIAGKHSKDIISILGYDYGSEVIHRDDMVVIQE
>Mature_378_residues
TTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSGAIAAGREHLGYPALPNAMASK
QLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDRERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNL
SALVGILCGADKLLLLTDQKGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVI
IAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVEKGSSLLAKGVTKVLGEFSRGE
VVRVTDAQGHLVARGIASYSNQDMAKIAGKHSKDIISILGYDYGSEVIHRDDMVVIQE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.1678321678322, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=31.9444444444444, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=65.2054794520548, Blast_Score=483, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17569969, Length=283, Percent_Identity=33.9222614840989, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=283, Percent_Identity=33.9222614840989, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=36.6906474820144, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=36.8932038834951, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.6460481099656, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_VIBVU (Q8DF93)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759328.1
- ProteinModelPortal:   Q8DF93
- SMR:   Q8DF93
- GeneID:   1177287
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0326
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   VVUL216895:VV1_0326-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40092; Mature: 39960

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSG
CCCCCCCHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAAGREHLGYPALPNAMASKQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDR
CCCCCHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNLSALVGILCGADKLLLLTDQ
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEE
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVE
EEECCCCCEEEEECCCCCCCCCEECCHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHH
KGSSLLAKGVTKVLGEFSRGEVVRVTDAQGHLVARGIASYSNQDMAKIAGKHSKDIISIL
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHH
GYDYGSEVIHRDDMVVIQE
CCCCCCHHEECCCEEEEEC
>Mature Secondary Structure 
TTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSG
CCCCCCHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAAGREHLGYPALPNAMASKQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDR
CCCCCHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNLSALVGILCGADKLLLLTDQ
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEE
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVE
EEECCCCCEEEEECCCCCCCCCEECCHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHH
KGSSLLAKGVTKVLGEFSRGEVVRVTDAQGHLVARGIASYSNQDMAKIAGKHSKDIISIL
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHH
GYDYGSEVIHRDDMVVIQE
CCCCCCHHEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA