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The map label for this gene is caiC [H]

Identifier: 37523767

GI number: 37523767

Start: 4394671

End: 4395264

Strand: Direct

Name: caiC [H]

Synonym: glr4198

Alternate gene names: 37523767

Gene position: 4394671-4395264 (Clockwise)

Preceding gene: 37523766

Following gene: 37523768

Centisome position: 94.33

GC content: 56.57

Gene sequence:

>594_bases
GTGCTGTCTAACGCTCTGGACAAAGTCGATACTCGTTTGCAGTCAAAAAATGCTCTCGTATTTGGCGAAGAATCCTATAC
TTACATGCAACTAAATAAACTTGTTGACCGCCTGGCTACCGCGTTGCTGAAGTGGAACGTTCAGGCCGGTGATCGCGTCG
CTTTTTTGATGCCGAACTGCCCCGAGATTGTGCTGTGCTACTACGCTTGCTTCAAGATCGGTGCCGTTGCAGTTCCGATC
AATATACGCTTCGGGGCGGATCTGGTCCGCTACGTGATGAACCACAGCGGTGCCCAAGTTCTGCTCGCCGAGCCCTCCCT
CTACGAGCAATACCAGCAGGCGGCACCTGCGCAGGCAACGGCTGCGCAGGTGCATTTGACACAATCGCTAGGGGCGCAAG
GATGGATAGCAAATTTGGCGGTTGAAGGCCCACCGCCCGCGTGGCCGGACATGGTGGCCGACCATCCCGCTGCGATTTTT
TACACTTCCGGGACGACAGGACAGCCAAAGGGCGTCATCCATACGCGCCGAAGCCTGGAGCAGGCCACCATCAACCAGAT
TCGCCAGATTGGCATCGACGCGCAGGACAGCTAG

Upstream 100 bases:

>100_bases
GATTACACGCGCCTCTCGCATCGCGTTTCCCGTTGTATTCTTGCTGGCAAATATTATTCTGGCATTTCTTTTCTTTGGAT
TTTAGCTCAAGGAGCCCGTC

Downstream 100 bases:

>100_bases
GGTGTATGACTGTATCGGTACTGACGACGGGCGCCGGGAAGCAAACCCGACTGCCGGGGGATTGATGCATGCGTTCTTAC
CTTTAAATTGGAACTGCCGA

Product: coronafacate ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLSNALDKVDTRLQSKNALVFGEESYTYMQLNKLVDRLATALLKWNVQAGDRVAFLMPNCPEIVLCYYACFKIGAVAVPI
NIRFGADLVRYVMNHSGAQVLLAEPSLYEQYQQAAPAQATAAQVHLTQSLGAQGWIANLAVEGPPPAWPDMVADHPAAIF
YTSGTTGQPKGVIHTRRSLEQATINQIRQIGIDAQDS

Sequences:

>Translated_197_residues
MLSNALDKVDTRLQSKNALVFGEESYTYMQLNKLVDRLATALLKWNVQAGDRVAFLMPNCPEIVLCYYACFKIGAVAVPI
NIRFGADLVRYVMNHSGAQVLLAEPSLYEQYQQAAPAQATAAQVHLTQSLGAQGWIANLAVEGPPPAWPDMVADHPAAIF
YTSGTTGQPKGVIHTRRSLEQATINQIRQIGIDAQDS
>Mature_197_residues
MLSNALDKVDTRLQSKNALVFGEESYTYMQLNKLVDRLATALLKWNVQAGDRVAFLMPNCPEIVLCYYACFKIGAVAVPI
NIRFGADLVRYVMNHSGAQVLLAEPSLYEQYQQAAPAQATAAQVHLTQSLGAQGWIANLAVEGPPPAWPDMVADHPAAIF
YTSGTTGQPKGVIHTRRSLEQATINQIRQIGIDAQDS

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI221142682, Length=164, Percent_Identity=33.5365853658537, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI145693145, Length=168, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1790505, Length=206, Percent_Identity=27.1844660194175, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 21516; Mature: 21516

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNALDKVDTRLQSKNALVFGEESYTYMQLNKLVDRLATALLKWNVQAGDRVAFLMPNC
CCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PEIVLCYYACFKIGAVAVPINIRFGADLVRYVMNHSGAQVLLAEPSLYEQYQQAAPAQAT
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCHHH
AAQVHLTQSLGAQGWIANLAVEGPPPAWPDMVADHPAAIFYTSGTTGQPKGVIHTRRSLE
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
QATINQIRQIGIDAQDS
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSNALDKVDTRLQSKNALVFGEESYTYMQLNKLVDRLATALLKWNVQAGDRVAFLMPNC
CCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PEIVLCYYACFKIGAVAVPINIRFGADLVRYVMNHSGAQVLLAEPSLYEQYQQAAPAQAT
HHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCHHH
AAQVHLTQSLGAQGWIANLAVEGPPPAWPDMVADHPAAIFYTSGTTGQPKGVIHTRRSLE
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
QATINQIRQIGIDAQDS
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA