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The map label for this gene is cls [H]

Identifier: 37523647

GI number: 37523647

Start: 4274017

End: 4275459

Strand: Reverse

Name: cls [H]

Synonym: gll4078

Alternate gene names: 37523647

Gene position: 4275459-4274017 (Counterclockwise)

Preceding gene: 37523648

Following gene: 37523646

Centisome position: 91.77

GC content: 62.09

Gene sequence:

>1443_bases
ATGCTCGCTCAGGAGGCGACACTTTTTAGCCTGCTGGTCCTTGCTGTCCATGGGCTGGGGATTGCCAACGCCGCCCATGC
CGTGATGCATGTGCGCTCATCTCGGGGGGCAGTGGCCTGGAGCATTGGGCTGATTACTTTTCCGTGGCTCGCCATCCCGC
TGTACTGGGTCTTTGGGCGCAACCGCTTTTTGGGCTACGCCGAGGCCATCCGTACCGCCTACGAGCAGCACCACCGCCGC
ATCGACAGAGCCTACGACGGCCTGCTGGAGCATCGAGCACAGCTTCCTGCGCGCTTGGCCCCGGTGGAACAACTGGGCGA
ACAACTCGCCAGTCTGCCGTTTTTGACCGGCAACACCGCCCGGTTGCTCATCGACGGCCGCGAAACGTACGACGCGATGC
TCTCGGCCATTGCCGGGGCCGAGCACTATGTGTTGTTGCAGTCGTACATCGTCGAGGCGGATCGCTCGGGTGAAGTGTTC
AAACAGGCGCTCATCGAGCGGGTCGGGGCCGGAGTGCGGGTGTATGTGCTCTACGACGAAATCGGCAGCAACCACCTGCC
CCGTTCCTACATCGACGATTTGCGCCGCCACGGCGTCGAGGTGAGTGCCTTTCACAGCACCAAGGGTTGGAAGAACCGGC
TGCAGATCAACTTTCGCAACCACACGAAGATCCTGGTGGTGGATGGTCGGGTCGCCTGCGTGGGCGGACTCAACATCGGC
GATGCATATCTTGGAGAGGGCAAAAACCCGCAGCTGCGCCCCTGGCGAGACACCCATTTGCGCCTCACCGGTCCCGCCGT
CCAGTGTTTGCAGTTGATTTTCCTGCGCGACTGGTACTGGGCGACGGGCGAATCGCCGCCGGTCGAATGGCGGGTGCACG
CCGAGCGGGAAGCGGGGGCGGCGGCCCTGGTGCTGCGCACCGGTCCGGCGGACAGCTTGCCCGCCTGCACGCTGTTTTTT
GTGGATCTGATCCAGCAGGCCCGCGAGCGCCTGTGGCTTGCCAGTCCTTACTTCGTGCCCCCGGAACCGGTGTTGCTTGC
CTTGCAACTGGCGGCGATGCGGGGAGTCGATGTGCGCATAATGCTCCCGGAGCATCCGGATCACCTGCTGGTCTATCTGT
GTGCCTTTTCGTTCTACGACGACCTGGCCCGCACCGACATCAAGCTCTACCGCTACCAGAAAGGTTTCATGCACCAGAAG
GTGATCCTGGTGGACCGCGAAATTGCCGGGGTCGGCACCGCCAACCTCGACAACCGCTCGTTCACCCTCAACTTCGAGGT
AATGAACTACATTGTGGATCCCGATTTTGTGAAAAGCGTCGAAGCGATGCTGATGGCGGATCTGGACGTCTGCCGCCAGG
TCGATTACCGGGAATACCGCCGCAAACCTTTGTGGTTTCGCCTCGCGGTGCGCATTTCCCGTCTCCTGGCACCGGTGCTG
TAG

Upstream 100 bases:

>100_bases
AGCGAGAAGGCGAACGCCACCCGGGTGCTCCGGCGCACCACCTGCTCCGACCACCGGCCGATTCTTACTGAACTGACCTG
GGACGAGGAGACCGACTGAA

Downstream 100 bases:

>100_bases
CGCATAATCGTTCCCAGCCTCCCGCAAAGGTGCAATGGAGCGATTCGCAAGGAGTGTTATGCAATCGAGACCGTGGCCGC
TGCTGCCGGTGTGGGCGGCG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLAQEATLFSLLVLAVHGLGIANAAHAVMHVRSSRGAVAWSIGLITFPWLAIPLYWVFGRNRFLGYAEAIRTAYEQHHRR
IDRAYDGLLEHRAQLPARLAPVEQLGEQLASLPFLTGNTARLLIDGRETYDAMLSAIAGAEHYVLLQSYIVEADRSGEVF
KQALIERVGAGVRVYVLYDEIGSNHLPRSYIDDLRRHGVEVSAFHSTKGWKNRLQINFRNHTKILVVDGRVACVGGLNIG
DAYLGEGKNPQLRPWRDTHLRLTGPAVQCLQLIFLRDWYWATGESPPVEWRVHAEREAGAAALVLRTGPADSLPACTLFF
VDLIQQARERLWLASPYFVPPEPVLLALQLAAMRGVDVRIMLPEHPDHLLVYLCAFSFYDDLARTDIKLYRYQKGFMHQK
VILVDREIAGVGTANLDNRSFTLNFEVMNYIVDPDFVKSVEAMLMADLDVCRQVDYREYRRKPLWFRLAVRISRLLAPVL

Sequences:

>Translated_480_residues
MLAQEATLFSLLVLAVHGLGIANAAHAVMHVRSSRGAVAWSIGLITFPWLAIPLYWVFGRNRFLGYAEAIRTAYEQHHRR
IDRAYDGLLEHRAQLPARLAPVEQLGEQLASLPFLTGNTARLLIDGRETYDAMLSAIAGAEHYVLLQSYIVEADRSGEVF
KQALIERVGAGVRVYVLYDEIGSNHLPRSYIDDLRRHGVEVSAFHSTKGWKNRLQINFRNHTKILVVDGRVACVGGLNIG
DAYLGEGKNPQLRPWRDTHLRLTGPAVQCLQLIFLRDWYWATGESPPVEWRVHAEREAGAAALVLRTGPADSLPACTLFF
VDLIQQARERLWLASPYFVPPEPVLLALQLAAMRGVDVRIMLPEHPDHLLVYLCAFSFYDDLARTDIKLYRYQKGFMHQK
VILVDREIAGVGTANLDNRSFTLNFEVMNYIVDPDFVKSVEAMLMADLDVCRQVDYREYRRKPLWFRLAVRISRLLAPVL
>Mature_480_residues
MLAQEATLFSLLVLAVHGLGIANAAHAVMHVRSSRGAVAWSIGLITFPWLAIPLYWVFGRNRFLGYAEAIRTAYEQHHRR
IDRAYDGLLEHRAQLPARLAPVEQLGEQLASLPFLTGNTARLLIDGRETYDAMLSAIAGAEHYVLLQSYIVEADRSGEVF
KQALIERVGAGVRVYVLYDEIGSNHLPRSYIDDLRRHGVEVSAFHSTKGWKNRLQINFRNHTKILVVDGRVACVGGLNIG
DAYLGEGKNPQLRPWRDTHLRLTGPAVQCLQLIFLRDWYWATGESPPVEWRVHAEREAGAAALVLRTGPADSLPACTLFF
VDLIQQARERLWLASPYFVPPEPVLLALQLAAMRGVDVRIMLPEHPDHLLVYLCAFSFYDDLARTDIKLYRYQKGFMHQK
VILVDREIAGVGTANLDNRSFTLNFEVMNYIVDPDFVKSVEAMLMADLDVCRQVDYREYRRKPLWFRLAVRISRLLAPVL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=493, Percent_Identity=28.3975659229209, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787007, Length=360, Percent_Identity=28.3333333333333, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081826, Length=437, Percent_Identity=24.9427917620137, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54423; Mature: 54423

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQEATLFSLLVLAVHGLGIANAAHAVMHVRSSRGAVAWSIGLITFPWLAIPLYWVFGR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC
NRFLGYAEAIRTAYEQHHRRIDRAYDGLLEHRAQLPARLAPVEQLGEQLASLPFLTGNTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCCCE
RLLIDGRETYDAMLSAIAGAEHYVLLQSYIVEADRSGEVFKQALIERVGAGVRVYVLYDE
EEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEC
IGSNHLPRSYIDDLRRHGVEVSAFHSTKGWKNRLQINFRNHTKILVVDGRVACVGGLNIG
CCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCEEEEEECCCEEEEECCCCC
DAYLGEGKNPQLRPWRDTHLRLTGPAVQCLQLIFLRDWYWATGESPPVEWRVHAEREAGA
CHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCCCC
AALVLRTGPADSLPACTLFFVDLIQQARERLWLASPYFVPPEPVLLALQLAAMRGVDVRI
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
MLPEHPDHLLVYLCAFSFYDDLARTDIKLYRYQKGFMHQKVILVDREIAGVGTANLDNRS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCE
FTLNFEVMNYIVDPDFVKSVEAMLMADLDVCRQVDYREYRRKPLWFRLAVRISRLLAPVL
EEEEEEHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAQEATLFSLLVLAVHGLGIANAAHAVMHVRSSRGAVAWSIGLITFPWLAIPLYWVFGR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC
NRFLGYAEAIRTAYEQHHRRIDRAYDGLLEHRAQLPARLAPVEQLGEQLASLPFLTGNTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCCCE
RLLIDGRETYDAMLSAIAGAEHYVLLQSYIVEADRSGEVFKQALIERVGAGVRVYVLYDE
EEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEC
IGSNHLPRSYIDDLRRHGVEVSAFHSTKGWKNRLQINFRNHTKILVVDGRVACVGGLNIG
CCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCEEEEEECCCEEEEECCCCC
DAYLGEGKNPQLRPWRDTHLRLTGPAVQCLQLIFLRDWYWATGESPPVEWRVHAEREAGA
CHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCCCC
AALVLRTGPADSLPACTLFFVDLIQQARERLWLASPYFVPPEPVLLALQLAAMRGVDVRI
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
MLPEHPDHLLVYLCAFSFYDDLARTDIKLYRYQKGFMHQKVILVDREIAGVGTANLDNRS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCE
FTLNFEVMNYIVDPDFVKSVEAMLMADLDVCRQVDYREYRRKPLWFRLAVRISRLLAPVL
EEEEEEHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]