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The map label for this gene is prfB [H]

Identifier: 37523609

GI number: 37523609

Start: 4238852

End: 4239229

Strand: Direct

Name: prfB [H]

Synonym: glr4040

Alternate gene names: 37523609

Gene position: 4238852-4239229 (Clockwise)

Preceding gene: 37523608

Following gene: 37523610

Centisome position: 90.98

GC content: 66.4

Gene sequence:

>378_bases
ATGGAACTGATTCGGAATCACGAGCGCGATTTGCCCGCATATCCCCTCGACCGGGAGGCGCTGGAGCGCGACTGTACTCT
GGAATTTGTGATTGCCAGTGGACCCGGCGGACAGCACCGCAACAAGACCGAAAGCGGCGTGCGGCTGACCCACCGCCCAA
GCGGCGTCGTGGTCAGCGCCACCGAGCGGCGCTCCCAGCACCAGAACCGGGAAGTGGCCTTCGAGCGCATGGCCGCCAAG
CTCGCCGATCTGCAGCGGGTTCGGGCTCCCCGCAAACCGACCCGCAAACCCCGGGCGGCCAAATTGCGCGATCTCGAAGC
GAAAAAACGCCGCTCCGCCATCAAGCGCGCCCGCTCCGGCCGCGATCTGGCCGAATAG

Upstream 100 bases:

>100_bases
GCCGGCCTCAACGACGTGCGCGTCTCGCGCAAAGAAGCGCGCAAGCGCGGCAGCATCGCTTAGCAGCCCATCCCTTAGAC
GTTCCTGCTAGCCTGTAAAT

Downstream 100 bases:

>100_bases
ACGCTGTGGAACGCTACTTTGCCACCGTCGCCCGCGGCCTCGAAGCGGTCGCCGCCGCCGAACTGGAGCGACTTGGGGCG
CAGCGGGTGGAAGCGGCCTT

Product: hypothetical protein

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MELIRNHERDLPAYPLDREALERDCTLEFVIASGPGGQHRNKTESGVRLTHRPSGVVVSATERRSQHQNREVAFERMAAK
LADLQRVRAPRKPTRKPRAAKLRDLEAKKRRSAIKRARSGRDLAE

Sequences:

>Translated_125_residues
MELIRNHERDLPAYPLDREALERDCTLEFVIASGPGGQHRNKTESGVRLTHRPSGVVVSATERRSQHQNREVAFERMAAK
LADLQRVRAPRKPTRKPRAAKLRDLEAKKRRSAIKRARSGRDLAE
>Mature_125_residues
MELIRNHERDLPAYPLDREALERDCTLEFVIASGPGGQHRNKTESGVRLTHRPSGVVVSATERRSQHQNREVAFERMAAK
LADLQRVRAPRKPTRKPRAAKLRDLEAKKRRSAIKRARSGRDLAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Escherichia coli, GI2367172, Length=78, Percent_Identity=42.3076923076923, Blast_Score=66, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321295, Length=50, Percent_Identity=60, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921226, Length=83, Percent_Identity=42.1686746987952, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 14299; Mature: 14299

Theoretical pI: Translated: 11.69; Mature: 11.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIRNHERDLPAYPLDREALERDCTLEFVIASGPGGQHRNKTESGVRLTHRPSGVVVSA
CCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEC
TERRSQHQNREVAFERMAAKLADLQRVRAPRKPTRKPRAAKLRDLEAKKRRSAIKRARSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RDLAE
CCCCC
>Mature Secondary Structure
MELIRNHERDLPAYPLDREALERDCTLEFVIASGPGGQHRNKTESGVRLTHRPSGVVVSA
CCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEC
TERRSQHQNREVAFERMAAKLADLQRVRAPRKPTRKPRAAKLRDLEAKKRRSAIKRARSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RDLAE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA