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The map label for this gene is pflB [H]

Identifier: 37523314

GI number: 37523314

Start: 3956164

End: 3957546

Strand: Direct

Name: pflB [H]

Synonym: glr3745

Alternate gene names: 37523314

Gene position: 3956164-3957546 (Clockwise)

Preceding gene: 37523311

Following gene: 37523315

Centisome position: 84.91

GC content: 61.97

Gene sequence:

>1383_bases
ATGATTCTAAACAAAGGTACTGCAAGGAAGTGGACTTGCGCAGGGGGCATGGCGGCAAAATTGCTGCCCCAACCCCAGGG
ATCACTAAGAAATAAGAACTTATTCAAACCGATTTCAAATTTCTTCAAATTACGTTGGGAAGCCGGTCGCTTCGGGGCGA
TGTTCTCGATACTGGGGGCATCGGTTGCGCTGGTTCCTACGGTGTCGAGAGCTGTGCCCAAACCTATACCCGCCGATCCC
TGGCAGGGCTTCCGACCAGGCAACTGGCAGGTGCGCACCGATGTCGAAAATTTTATTGCCGCCAACTGCGACCCCTACCT
GGGCGACGACAGCGATTTGACCGGTCCGACCGAGCGCACCCAGGCGCTCTGGGTGCAGGCGCAGATGCTTCAGCAGGCGG
CGCGGGCCACCGCCCGGCGCAGTGCCGGTCCAGGCGGAACCCCGGCAGGCGCGTTCGGCGCCGACTACCTCGATCGGCGC
GAAGAACTGATCTATGGCCTGATCGATCCGCTCGATGTCGAGCAGCTGCCGGTGGGGAGCGATTGTCGGCAGTTGGCCCT
GTACGGGTCGGAGCGATTGCGCCGATTGGGCATGCAGTTGGGGGCCGTCGAGACGGTCGAAGCGCTCGCCGGTTGCTACG
GCCTCGATATCGGCAGACCGGCGGCCACCGCCCAGCAGGCTATCCAGTGGACGTATCTGGCGCTGTTGGCCACCCGCGAT
CCGCTTTTTGGCCGGCGCCGTAGCGTCACTGGTCTCGACACGTTTTTTGATGTTTACTTCCAGCGCGACCTCGCTCGCGG
TTTGTTGGTCGAAGAAGAAGCCCAGGAACTGGTAGACGACCTGATGATCAAGTTGCGGTTGCCCCAGCCGACGCTGAGCG
AACTGTACCTGCCCCTGGCTGCGGGCGTAGACCCAGAAGGCAGGCCGCTGGTCACCCGCACCGACTTTCGATTCGTCAAC
ACCTGTTACAACCTGGGACCGGCCGCCGAACCGCACCTGCTGGTGCTCTGGTCGGCGGCCTTGAGCGGGGCGTTCCGGCG
CTTCTGCGGCGAGGTGACCATCGACACCAGTTCGCTGCGCTACGCCAGAGCACTACCGCTGCCGCAGACGGGTCTGGCCC
ACACTTTCAGTCCCGACGCTTTGGGCGCCACCCGCACCGAGCAAGTCGAGAACCTGGGCCTTTGGCTCGACGGGCAACCG
GAAGGCGATCACCTGGGAATCAACGTCTTTGGCCGTGAAGCATTGCTTTCGACCCTTACCCGTCCCAACAAGTTTGCCCA
TCTGACCGTGCAACTTGCCGAAGGGGCGGTAGATCTGCACAGCCTCAGCCCGCTCGGACGGCGCCAGGTACTTCTACAAT
TGCTGGGAAGCTTCGAAAGGTAA

Upstream 100 bases:

>100_bases
CAGCGCTGAAGCGGCGCATCCGCAAATGCCAGAGATTCAAGAAATCCCCAGACAAAACTGCCGGTGGTCCAATGCTGCAC
TACGGCCATGCCCCTTCCGA

Downstream 100 bases:

>100_bases
GGCGCGTGGATCCAACGCTGGCGGTCGGGCTGATCAGCGGCACTTCGCTAGACGGCATGGACGGGGCACTGGTAGAAATT
GCAGGCAGCGCGCAGCAGCC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MILNKGTARKWTCAGGMAAKLLPQPQGSLRNKNLFKPISNFFKLRWEAGRFGAMFSILGASVALVPTVSRAVPKPIPADP
WQGFRPGNWQVRTDVENFIAANCDPYLGDDSDLTGPTERTQALWVQAQMLQQAARATARRSAGPGGTPAGAFGADYLDRR
EELIYGLIDPLDVEQLPVGSDCRQLALYGSERLRRLGMQLGAVETVEALAGCYGLDIGRPAATAQQAIQWTYLALLATRD
PLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMIKLRLPQPTLSELYLPLAAGVDPEGRPLVTRTDFRFVN
TCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTSSLRYARALPLPQTGLAHTFSPDALGATRTEQVENLGLWLDGQP
EGDHLGINVFGREALLSTLTRPNKFAHLTVQLAEGAVDLHSLSPLGRRQVLLQLLGSFER

Sequences:

>Translated_460_residues
MILNKGTARKWTCAGGMAAKLLPQPQGSLRNKNLFKPISNFFKLRWEAGRFGAMFSILGASVALVPTVSRAVPKPIPADP
WQGFRPGNWQVRTDVENFIAANCDPYLGDDSDLTGPTERTQALWVQAQMLQQAARATARRSAGPGGTPAGAFGADYLDRR
EELIYGLIDPLDVEQLPVGSDCRQLALYGSERLRRLGMQLGAVETVEALAGCYGLDIGRPAATAQQAIQWTYLALLATRD
PLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMIKLRLPQPTLSELYLPLAAGVDPEGRPLVTRTDFRFVN
TCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTSSLRYARALPLPQTGLAHTFSPDALGATRTEQVENLGLWLDGQP
EGDHLGINVFGREALLSTLTRPNKFAHLTVQLAEGAVDLHSLSPLGRRQVLLQLLGSFER
>Mature_460_residues
MILNKGTARKWTCAGGMAAKLLPQPQGSLRNKNLFKPISNFFKLRWEAGRFGAMFSILGASVALVPTVSRAVPKPIPADP
WQGFRPGNWQVRTDVENFIAANCDPYLGDDSDLTGPTERTQALWVQAQMLQQAARATARRSAGPGGTPAGAFGADYLDRR
EELIYGLIDPLDVEQLPVGSDCRQLALYGSERLRRLGMQLGAVETVEALAGCYGLDIGRPAATAQQAIQWTYLALLATRD
PLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMIKLRLPQPTLSELYLPLAAGVDPEGRPLVTRTDFRFVN
TCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTSSLRYARALPLPQTGLAHTFSPDALGATRTEQVENLGLWLDGQP
EGDHLGINVFGREALLSTLTRPNKFAHLTVQLAEGAVDLHSLSPLGRRQVLLQLLGSFER

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=182, Percent_Identity=43.4065934065934, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI48994926, Length=222, Percent_Identity=35.5855855855856, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 50409; Mature: 50409

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNKGTARKWTCAGGMAAKLLPQPQGSLRNKNLFKPISNFFKLRWEAGRFGAMFSILGA
CCCCCCCCCEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
SVALVPTVSRAVPKPIPADPWQGFRPGNWQVRTDVENFIAANCDPYLGDDSDLTGPTERT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCHHHH
QALWVQAQMLQQAARATARRSAGPGGTPAGAFGADYLDRREELIYGLIDPLDVEQLPVGS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DCRQLALYGSERLRRLGMQLGAVETVEALAGCYGLDIGRPAATAQQAIQWTYLALLATRD
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
PLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMIKLRLPQPTLSELYLPLA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHH
AGVDPEGRPLVTRTDFRFVNTCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTSSLR
CCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCH
YARALPLPQTGLAHTFSPDALGATRTEQVENLGLWLDGQPEGDHLGINVFGREALLSTLT
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCEEEEEECCHHHHHHHHC
RPNKFAHLTVQLAEGAVDLHSLSPLGRRQVLLQLLGSFER
CCCCEEEEEEEECCCCEEHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILNKGTARKWTCAGGMAAKLLPQPQGSLRNKNLFKPISNFFKLRWEAGRFGAMFSILGA
CCCCCCCCCEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
SVALVPTVSRAVPKPIPADPWQGFRPGNWQVRTDVENFIAANCDPYLGDDSDLTGPTERT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCHHHH
QALWVQAQMLQQAARATARRSAGPGGTPAGAFGADYLDRREELIYGLIDPLDVEQLPVGS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DCRQLALYGSERLRRLGMQLGAVETVEALAGCYGLDIGRPAATAQQAIQWTYLALLATRD
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
PLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMIKLRLPQPTLSELYLPLA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHH
AGVDPEGRPLVTRTDFRFVNTCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTSSLR
CCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCH
YARALPLPQTGLAHTFSPDALGATRTEQVENLGLWLDGQPEGDHLGINVFGREALLSTLT
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCEEEEEECCHHHHHHHHC
RPNKFAHLTVQLAEGAVDLHSLSPLGRRQVLLQLLGSFER
CCCCEEEEEEEECCCCEEHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]