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The map label for this gene is gshB

Identifier: 37523251

GI number: 37523251

Start: 3889817

End: 3890791

Strand: Reverse

Name: gshB

Synonym: gvip498

Alternate gene names: 37523251

Gene position: 3890791-3889817 (Counterclockwise)

Preceding gene: 37523252

Following gene: 37523250

Centisome position: 83.51

GC content: 63.28

Gene sequence:

>975_bases
ATGAAGATTCTTTTTATCGTCGATCCGCTCGAGACCCTTAAACCCGGTCACGACACTTCGGTTGCCCTGATGCAGGCCGC
CGCGCGCCGCGGCCACAGCGTTTGGGCGGCGGAGGTGGGCGATTTGCAGGTCCACCACCACCGCGCCGCCGCCCAGGTGC
GCACCCTGACGATCCATCCGGGCCGCACGCCGTTCTATACGGTCGAAGCCGTCGGCTTTTACCCCCTGAGCGAAGCCGAC
GTCATCTGGATGCGCAAAGATCCGCCGGTCACCTCCGCCTATCTCTGGGCCACGCAGGTTCTCGATCTGGTCAACGCCGG
ACGCGGCGACGGGCGCACGACTTTTGTGCTCAACCGGCCCTCGGGATTGCGCAACGACAACGAAAAGCTCTACGCCCTGC
ACTTTCCCGACCTGGTCCCCGAGACGCGGGTCTGTACCCACCGCCAGGACATCCTCGATTTTGTCGATATCCACGGCCGG
GCGGTGATCAAGCCCCTCGACGGCAAGGGCGGCGAAGGAATTTTTCTGCTCGCGCGCGCCGATCGCAATCTCAACGCGAT
CATCGAGGCGAGCACCGCCTACGGCACCCGCCATGTCATGGTGCAGCGCTACCTCGAAGAATCGCGCCAGGGCGACAAGC
GCATCGTGCTTTTGGCCGGTGAACCGATCGGCGCTCTGTTGCGCGTTCCGCGCGAGGACGACGTGCGCGGCAACATGGCC
GCCGGGGGCCGAGTGGTCAAAACCACCCTCACCGAGCGCGACCGCGAAATCTGCCGGGTGGTCGGACCGCGGCTGGTGGC
CGACGGCCACTACTTTGTCGGCATCGATGTGATCGGTGCTTACCTCACCGAGATCAATGTCACCAGCCCGACTGGAATCT
GCGAAATCGATGTGCTGGATGGTGTAGTGTTGGAGGATGAAATCGTCGACTGGCTGGTCGAATATACACGCCCCGCACTC
GCAAGGAACCTCTAA

Upstream 100 bases:

>100_bases
GTGTGCCGCAGATATTTATCGACGGCAAGCACATCGGCGGTTGCGACGATCTATACGCCCTCGATCGCTCCGGCCAGCTC
GATCCGCTGCTTGTCGCTTC

Downstream 100 bases:

>100_bases
ATTGTTGCAAGCCACGGCATACAAACCTTTGTACAATTGTCGGCCTTAGGGAGGTCCGTATGTTGCATCGCAAGATTTAC
CAGCTCTCCCGCGAAGGGAC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKILFIVDPLETLKPGHDTSVALMQAAARRGHSVWAAEVGDLQVHHHRAAAQVRTLTIHPGRTPFYTVEAVGFYPLSEAD
VIWMRKDPPVTSAYLWATQVLDLVNAGRGDGRTTFVLNRPSGLRNDNEKLYALHFPDLVPETRVCTHRQDILDFVDIHGR
AVIKPLDGKGGEGIFLLARADRNLNAIIEASTAYGTRHVMVQRYLEESRQGDKRIVLLAGEPIGALLRVPREDDVRGNMA
AGGRVVKTTLTERDREICRVVGPRLVADGHYFVGIDVIGAYLTEINVTSPTGICEIDVLDGVVLEDEIVDWLVEYTRPAL
ARNL

Sequences:

>Translated_324_residues
MKILFIVDPLETLKPGHDTSVALMQAAARRGHSVWAAEVGDLQVHHHRAAAQVRTLTIHPGRTPFYTVEAVGFYPLSEAD
VIWMRKDPPVTSAYLWATQVLDLVNAGRGDGRTTFVLNRPSGLRNDNEKLYALHFPDLVPETRVCTHRQDILDFVDIHGR
AVIKPLDGKGGEGIFLLARADRNLNAIIEASTAYGTRHVMVQRYLEESRQGDKRIVLLAGEPIGALLRVPREDDVRGNMA
AGGRVVKTTLTERDREICRVVGPRLVADGHYFVGIDVIGAYLTEINVTSPTGICEIDVLDGVVLEDEIVDWLVEYTRPAL
ARNL
>Mature_324_residues
MKILFIVDPLETLKPGHDTSVALMQAAARRGHSVWAAEVGDLQVHHHRAAAQVRTLTIHPGRTPFYTVEAVGFYPLSEAD
VIWMRKDPPVTSAYLWATQVLDLVNAGRGDGRTTFVLNRPSGLRNDNEKLYALHFPDLVPETRVCTHRQDILDFVDIHGR
AVIKPLDGKGGEGIFLLARADRNLNAIIEASTAYGTRHVMVQRYLEESRQGDKRIVLLAGEPIGALLRVPREDDVRGNMA
AGGRVVKTTLTERDREICRVVGPRLVADGHYFVGIDVIGAYLTEINVTSPTGICEIDVLDGVVLEDEIVDWLVEYTRPAL
ARNL

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=298, Percent_Identity=40.6040268456376, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787076, Length=258, Percent_Identity=25.1937984496124, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_GLOVI (Q7NF44)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926628.1
- ProteinModelPortal:   Q7NF44
- SMR:   Q7NF44
- GeneID:   2602086
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3682
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BioCyc:   GVIO251221:GLL3682-MONOMER
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35907; Mature: 35907

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFIVDPLETLKPGHDTSVALMQAAARRGHSVWAAEVGDLQVHHHRAAAQVRTLTIHP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEHHHHHCEEEEEEECC
GRTPFYTVEAVGFYPLSEADVIWMRKDPPVTSAYLWATQVLDLVNAGRGDGRTTFVLNRP
CCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SGLRNDNEKLYALHFPDLVPETRVCTHRQDILDFVDIHGRAVIKPLDGKGGEGIFLLARA
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
DRNLNAIIEASTAYGTRHVMVQRYLEESRQGDKRIVLLAGEPIGALLRVPREDDVRGNMA
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHEECCCCCCCCCCCC
AGGRVVKTTLTERDREICRVVGPRLVADGHYFVGIDVIGAYLTEINVTSPTGICEIDVLD
CCCEEEEEHHHHHHHHHHHHHCCEEEECCCEEEEHHHHHHHHHEEECCCCCCEEEEEECC
GVVLEDEIVDWLVEYTRPALARNL
CEEEHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MKILFIVDPLETLKPGHDTSVALMQAAARRGHSVWAAEVGDLQVHHHRAAAQVRTLTIHP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEHHHHHCEEEEEEECC
GRTPFYTVEAVGFYPLSEADVIWMRKDPPVTSAYLWATQVLDLVNAGRGDGRTTFVLNRP
CCCCEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SGLRNDNEKLYALHFPDLVPETRVCTHRQDILDFVDIHGRAVIKPLDGKGGEGIFLLARA
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
DRNLNAIIEASTAYGTRHVMVQRYLEESRQGDKRIVLLAGEPIGALLRVPREDDVRGNMA
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHEECCCCCCCCCCCC
AGGRVVKTTLTERDREICRVVGPRLVADGHYFVGIDVIGAYLTEINVTSPTGICEIDVLD
CCCEEEEEHHHHHHHHHHHHHCCEEEECCCEEEEHHHHHHHHHEEECCCCCCEEEEEECC
GVVLEDEIVDWLVEYTRPALARNL
CEEEHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292