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The map label for this gene is gcvH

Identifier: 37523100

GI number: 37523100

Start: 3730724

End: 3731113

Strand: Direct

Name: gcvH

Synonym: glr3531

Alternate gene names: 37523100

Gene position: 3730724-3731113 (Clockwise)

Preceding gene: 37523099

Following gene: 37523101

Centisome position: 80.08

GC content: 60.77

Gene sequence:

>390_bases
ATGGCCCTGGAATACCCGGAGGAACTAAAATATCTCGACTCCCACGAGTACCTGCGCGTCGAGGGAGACACCGTGGTGGT
GGGCGTCACGTCCTATGCCGTCGATCAACTCGGCGACATCGTCTTTGTCTCGCTTCCGGAGGAAGGCGATCGCATCAACC
GCGGCGACAGCTTCGGTTCGATCGAATCGGTCAAGGCGGTCGAAGAACTGTACGCCCCGCTCTCCGGCACGGTTTTGTCG
GTCAACACCGTCGCAGTCGAAGATCCGGCGCTTATCGGTAGCGATCCCTATGGCGACGGTTGGCTCATCAAGGTCCGTCT
CGCCGACCCTGCCGACGAGTTGGGCGAAACGATGACTGCCGAGGAATACCGCGAGCGCGTCGAAGGCTGA

Upstream 100 bases:

>100_bases
ATTCGCGGCCGCCGGGCCATGGCCACCGTCGTCAAGCGTCCCTTCTACCGCCGCGCGCTGTAAGATCCCTGTGTGACAAA
AATGCAGAGGTAGACGAACC

Downstream 100 bases:

>100_bases
GCAAAATGCTGCGCACATCGTACAGTTGCAAGAAACGCTCGATCCTGGTCGCCTCGACCTTGCTGCTTGTGGCGGGCTGC
GGCAACAGCCCAAAGCTGGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MALEYPEELKYLDSHEYLRVEGDTVVVGVTSYAVDQLGDIVFVSLPEEGDRINRGDSFGSIESVKAVEELYAPLSGTVLS
VNTVAVEDPALIGSDPYGDGWLIKVRLADPADELGETMTAEEYRERVEG

Sequences:

>Translated_129_residues
MALEYPEELKYLDSHEYLRVEGDTVVVGVTSYAVDQLGDIVFVSLPEEGDRINRGDSFGSIESVKAVEELYAPLSGTVLS
VNTVAVEDPALIGSDPYGDGWLIKVRLADPADELGETMTAEEYRERVEG
>Mature_128_residues
ALEYPEELKYLDSHEYLRVEGDTVVVGVTSYAVDQLGDIVFVSLPEEGDRINRGDSFGSIESVKAVEELYAPLSGTVLSV
NTVAVEDPALIGSDPYGDGWLIKVRLADPADELGETMTAEEYRERVEG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=43.6974789915966, Blast_Score=114, Evalue=3e-26,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=42.8571428571429, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=43.5483870967742, Blast_Score=103, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=45.1327433628319, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=40.8695652173913, Blast_Score=101, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=42.4, Blast_Score=109, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=42.8571428571429, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_GLOVI (Q7NFJ4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926477.1
- HSSP:   P16048
- ProteinModelPortal:   Q7NFJ4
- SMR:   Q7NFJ4
- GeneID:   2601935
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr3531
- NMPDR:   fig|251221.1.peg.3531
- HOGENOM:   HBG693789
- OMA:   VIGITAY
- ProtClustDB:   PRK01202
- BioCyc:   GVIO251221:GLR3531-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14100; Mature: 13969

Theoretical pI: Translated: 3.79; Mature: 3.79

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEYPEELKYLDSHEYLRVEGDTVVVGVTSYAVDQLGDIVFVSLPEEGDRINRGDSFGS
CCCCCCHHHHHCCCCCEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
IESVKAVEELYAPLSGTVLSVNTVAVEDPALIGSDPYGDGWLIKVRLADPADELGETMTA
HHHHHHHHHHHCCCCCCEEEEEEEEEECCCEECCCCCCCCEEEEEEECCCHHHHCCCCCH
EEYRERVEG
HHHHHHHCC
>Mature Secondary Structure 
ALEYPEELKYLDSHEYLRVEGDTVVVGVTSYAVDQLGDIVFVSLPEEGDRINRGDSFGS
CCCCCHHHHHCCCCCEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
IESVKAVEELYAPLSGTVLSVNTVAVEDPALIGSDPYGDGWLIKVRLADPADELGETMTA
HHHHHHHHHHHCCCCCCEEEEEEEEEECCCEECCCCCCCCEEEEEEECCCHHHHCCCCCH
EEYRERVEG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292