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The map label for this gene is ppnK

Identifier: 37523094

GI number: 37523094

Start: 3726339

End: 3727268

Strand: Reverse

Name: ppnK

Synonym: gll3525

Alternate gene names: 37523094

Gene position: 3727268-3726339 (Counterclockwise)

Preceding gene: 37523095

Following gene: 37523093

Centisome position: 80.0

GC content: 65.59

Gene sequence:

>930_bases
ATGATGGCCCTCCCCAAAGCCGGGATCATCTTCAACGACATCAAACCGGCCGCCGCCCGGGCGGCGGCGGAATTAACCGA
AAAATTCCAGGACGCGGGCTACCGCGTCTATCAGACCACCGGCTGGGGAGGCATCCTGGGTTTCCCTCGCCCGGACAGCC
CCGTCTGCCATACCCCCATCGACCGGCTCGCGGCCGAGGGTTTCGACGAGGCGATGCCCTTCGCCATCGTGCTGGGGGGC
GACGGCACGGTGCTGGCCGCCGCCCGCCAGGTGGCCCCCTTCGATATTCCCTTGCTGACCATCAACACTGGCCACATGGG
CTTTCTCACCGAGGGTTACCTCAATCAAATCCACCCGGCCATCGATACGCTGCTCGCCGGCCAGTACGCCCTCGAAGACC
GTTCGATGATCGAGGTGCGCGTCTTTCGCGACGAGCGGCTCATCTGGGAAGCGCTCGCCCTCAACGAGGCGGTGCTGCAC
AAAGAACCGCTCTCGGGGATCTGCCACTTCGAAGTGGCGATTGGCCGCCACAACATCGTCGATATCGCCGCTGACGGGGT
GATCGTCGCCACCCCCACCGGCTCCACCGCCTACGCCCTCTCCGCCGGGGGACCGGTGATCACCCCCGACGTGCAGGTAC
TGCAGCTCATCCCCATCTGTCCCCACTCCCTCGCCGCGCGGGGGCTGGTTTTTGCCGACACCGAATCGCTGGTGGTCCAT
CCCCCCACCAACCACCAGCACCTGATTCTTTCGCTCGACGGCAACTCCGGCTGCTATATCTGGCCGGGCGATCAGGTGCG
CATCCGCCGCGCCCGCTACCGCACCCGGCTAATCCGTCTGCAGCCGCCCGAATTTTTTGCGCTGCTGCGCGAGAAGCTCG
GCTGGGGCTTGCCGCACATCGCCAAACCCCTCTCGGTTGAACTGCCCTGA

Upstream 100 bases:

>100_bases
TTTGCCGAACTGGGCGCCGTCGCCCGCGATTTGGGCTTTGCCCACGTGCGCTCCGGACCGCTGGTGCGCAGTTCCTACCA
CGCCGGCGAGCCTGAATAGC

Downstream 100 bases:

>100_bases
CGCACAGCACAGCTTGCCTGCGTACACTCGTGATAGGCAGCGGCAAGCAGCGATGGATACCGGTAGAGCACCTTCCCTTG
AAGAATTGCGCCGACGGAGA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MMALPKAGIIFNDIKPAAARAAAELTEKFQDAGYRVYQTTGWGGILGFPRPDSPVCHTPIDRLAAEGFDEAMPFAIVLGG
DGTVLAAARQVAPFDIPLLTINTGHMGFLTEGYLNQIHPAIDTLLAGQYALEDRSMIEVRVFRDERLIWEALALNEAVLH
KEPLSGICHFEVAIGRHNIVDIAADGVIVATPTGSTAYALSAGGPVITPDVQVLQLIPICPHSLAARGLVFADTESLVVH
PPTNHQHLILSLDGNSGCYIWPGDQVRIRRARYRTRLIRLQPPEFFALLREKLGWGLPHIAKPLSVELP

Sequences:

>Translated_309_residues
MMALPKAGIIFNDIKPAAARAAAELTEKFQDAGYRVYQTTGWGGILGFPRPDSPVCHTPIDRLAAEGFDEAMPFAIVLGG
DGTVLAAARQVAPFDIPLLTINTGHMGFLTEGYLNQIHPAIDTLLAGQYALEDRSMIEVRVFRDERLIWEALALNEAVLH
KEPLSGICHFEVAIGRHNIVDIAADGVIVATPTGSTAYALSAGGPVITPDVQVLQLIPICPHSLAARGLVFADTESLVVH
PPTNHQHLILSLDGNSGCYIWPGDQVRIRRARYRTRLIRLQPPEFFALLREKLGWGLPHIAKPLSVELP
>Mature_309_residues
MMALPKAGIIFNDIKPAAARAAAELTEKFQDAGYRVYQTTGWGGILGFPRPDSPVCHTPIDRLAAEGFDEAMPFAIVLGG
DGTVLAAARQVAPFDIPLLTINTGHMGFLTEGYLNQIHPAIDTLLAGQYALEDRSMIEVRVFRDERLIWEALALNEAVLH
KEPLSGICHFEVAIGRHNIVDIAADGVIVATPTGSTAYALSAGGPVITPDVQVLQLIPICPHSLAARGLVFADTESLVVH
PPTNHQHLILSLDGNSGCYIWPGDQVRIRRARYRTRLIRLQPPEFFALLREKLGWGLPHIAKPLSVELP

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=264, Percent_Identity=30.6818181818182, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1788968, Length=223, Percent_Identity=37.219730941704, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320794, Length=253, Percent_Identity=29.2490118577075, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322509, Length=249, Percent_Identity=27.710843373494, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325068, Length=158, Percent_Identity=31.0126582278481, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573828, Length=263, Percent_Identity=30.0380228136882, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28573830, Length=263, Percent_Identity=30.0380228136882, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573826, Length=263, Percent_Identity=30.0380228136882, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573832, Length=263, Percent_Identity=30.0380228136882, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161077047, Length=263, Percent_Identity=30.0380228136882, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20129957, Length=285, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653422, Length=285, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363321, Length=285, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363323, Length=285, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653424, Length=285, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_GLOVI (Q7NFK0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926471.1
- ProteinModelPortal:   Q7NFK0
- GeneID:   2601929
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3525
- NMPDR:   fig|251221.1.peg.3525
- HOGENOM:   HBG713904
- OMA:   LNEMVLH
- ProtClustDB:   PRK02649
- BioCyc:   GVIO251221:GLL3525-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33606; Mature: 33606

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMALPKAGIIFNDIKPAAARAAAELTEKFQDAGYRVYQTTGWGGILGFPRPDSPVCHTPI
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCCCCCCCCCH
DRLAAEGFDEAMPFAIVLGGDGTVLAAARQVAPFDIPLLTINTGHMGFLTEGYLNQIHPA
HHHHHCCCCCCCCEEEEECCCCEEEEEHHHCCCCCEEEEEEECCCCCEECHHHHHHHHHH
IDTLLAGQYALEDRSMIEVRVFRDERLIWEALALNEAVLHKEPLSGICHFEVAIGRHNIV
HHHHHCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
DIAADGVIVATPTGSTAYALSAGGPVITPDVQVLQLIPICPHSLAARGLVFADTESLVVH
EEECCCEEEECCCCCCEEEEECCCCEECCCCEEEEEECCCCHHHHHCCEEEECCCCEEEE
PPTNHQHLILSLDGNSGCYIWPGDQVRIRRARYRTRLIRLQPPEFFALLREKLGWGLPHI
CCCCCCEEEEEEECCCCEEEECCCCEEHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCHH
AKPLSVELP
CCCCEECCC
>Mature Secondary Structure
MMALPKAGIIFNDIKPAAARAAAELTEKFQDAGYRVYQTTGWGGILGFPRPDSPVCHTPI
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCCCCCCCCCH
DRLAAEGFDEAMPFAIVLGGDGTVLAAARQVAPFDIPLLTINTGHMGFLTEGYLNQIHPA
HHHHHCCCCCCCCEEEEECCCCEEEEEHHHCCCCCEEEEEEECCCCCEECHHHHHHHHHH
IDTLLAGQYALEDRSMIEVRVFRDERLIWEALALNEAVLHKEPLSGICHFEVAIGRHNIV
HHHHHCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
DIAADGVIVATPTGSTAYALSAGGPVITPDVQVLQLIPICPHSLAARGLVFADTESLVVH
EEECCCEEEECCCCCCEEEEECCCCEECCCCEEEEEECCCCHHHHHCCEEEECCCCEEEE
PPTNHQHLILSLDGNSGCYIWPGDQVRIRRARYRTRLIRLQPPEFFALLREKLGWGLPHI
CCCCCCEEEEEEECCCCEEEECCCCEEHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCHH
AKPLSVELP
CCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292