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The map label for this gene is lipA2

Identifier: 37523095

GI number: 37523095

Start: 3727270

End: 3728139

Strand: Reverse

Name: lipA2

Synonym: gll3526

Alternate gene names: 37523095

Gene position: 3728139-3727270 (Counterclockwise)

Preceding gene: 37523098

Following gene: 37523094

Centisome position: 80.02

GC content: 66.44

Gene sequence:

>870_bases
GTGCTCGCCATGTCCCACCAGACTGCCAGCTCCCGCCTGCCCGAGTGGGCGCGCCGCACCATCGGCACTGCCTCGCAAGT
CTCGACGGTCGAGAAGATCGTCAAGCAGCGCTCGCTGCACACCATCTGCGAGGAGGGGCGCTGCCCGAACCGGGCGGAGT
GCTACGCCCAGAAGACAGCGACGTTTTTGTTGATGGGACCGGTGTGCACCCGCGCCTGCGGCTTCTGCCAGGTTGCCGGC
GGCAAGGCTCTTCCCCTCGACCCGCAGGAGCCTGAAAAAGTCGCCGAGGCGGTGGCGCTGTTGGGACTCAAGTACGTGGT
GCTCACTTCGGTGGCCCGCGACGACCTGCCGGATCAGGGGGCGGGCTGGTTTGTGCGCACGATGGAATCTATTCGCGCGC
GCAACTGCGCGGTCGAGATCGAAGTACTCACCCCGGATTTTCGCGGCGAAGAGGCGTGCATCGCCACCGTGGTCGCCGCG
CGACCCGTTTGCTTTAATCACAACATCGAGACGGTGCGGCGCCTGCAAAGCTACGCCCGCCGCGCCGCCACCTACGCGCG
CAGTCTGGCGGTCCTTGCCGCCGTCAAGCGTCTGGATGCGGAGATCTTCACCAAGTCGGGGCTGATGCTGGGCCACGGCG
AGACGCGCGAAGAAGTGCTCGAGACGTTGCTCGACCTGCGCAAGGTGGGTTGCGACCGGGTCACCCTCGGCCAGTACCTC
CAGCCTTCCAAAGACCACCTGCCGGTGCACAAGTACTGGACCCCGGCCGAATTTGCCGAACTGGGCGCCGTCGCCCGCGA
TTTGGGCTTTGCCCACGTGCGCTCCGGACCGCTGGTGCGCAGTTCCTACCACGCCGGCGAGCCTGAATAG

Upstream 100 bases:

>100_bases
GACTTCGAGGTTGTGGACAGATTCGATCCTGTTTTTTGTTTAAAGACAGGGCTTGAAACCCTTTCGTGCGTGTCAAGCTA
TCCTGAAAGGCGATCCGTCC

Downstream 100 bases:

>100_bases
CATGATGGCCCTCCCCAAAGCCGGGATCATCTTCAACGACATCAAACCGGCCGCCGCCCGGGCGGCGGCGGAATTAACCG
AAAAATTCCAGGACGCGGGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLAMSHQTASSRLPEWARRTIGTASQVSTVEKIVKQRSLHTICEEGRCPNRAECYAQKTATFLLMGPVCTRACGFCQVAG
GKALPLDPQEPEKVAEAVALLGLKYVVLTSVARDDLPDQGAGWFVRTMESIRARNCAVEIEVLTPDFRGEEACIATVVAA
RPVCFNHNIETVRRLQSYARRAATYARSLAVLAAVKRLDAEIFTKSGLMLGHGETREEVLETLLDLRKVGCDRVTLGQYL
QPSKDHLPVHKYWTPAEFAELGAVARDLGFAHVRSGPLVRSSYHAGEPE

Sequences:

>Translated_289_residues
MLAMSHQTASSRLPEWARRTIGTASQVSTVEKIVKQRSLHTICEEGRCPNRAECYAQKTATFLLMGPVCTRACGFCQVAG
GKALPLDPQEPEKVAEAVALLGLKYVVLTSVARDDLPDQGAGWFVRTMESIRARNCAVEIEVLTPDFRGEEACIATVVAA
RPVCFNHNIETVRRLQSYARRAATYARSLAVLAAVKRLDAEIFTKSGLMLGHGETREEVLETLLDLRKVGCDRVTLGQYL
QPSKDHLPVHKYWTPAEFAELGAVARDLGFAHVRSGPLVRSSYHAGEPE
>Mature_289_residues
MLAMSHQTASSRLPEWARRTIGTASQVSTVEKIVKQRSLHTICEEGRCPNRAECYAQKTATFLLMGPVCTRACGFCQVAG
GKALPLDPQEPEKVAEAVALLGLKYVVLTSVARDDLPDQGAGWFVRTMESIRARNCAVEIEVLTPDFRGEEACIATVVAA
RPVCFNHNIETVRRLQSYARRAATYARSLAVLAAVKRLDAEIFTKSGLMLGHGETREEVLETLLDLRKVGCDRVTLGQYL
QPSKDHLPVHKYWTPAEFAELGAVARDLGFAHVRSGPLVRSSYHAGEPE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=44.5229681978799, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=43.0894308943089, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=46.0144927536232, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=42.6573426573427, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=292, Percent_Identity=41.4383561643836, Blast_Score=222, Evalue=4e-59,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.9964664310954, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA2_GLOVI (Q7NFJ9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926472.1
- ProteinModelPortal:   Q7NFJ9
- GeneID:   2601930
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3526
- NMPDR:   fig|251221.1.peg.3526
- HOGENOM:   HBG284542
- OMA:   KTKIPMG
- ProtClustDB:   PRK12928
- BioCyc:   GVIO251221:GLL3526-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 31777; Mature: 31777

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAMSHQTASSRLPEWARRTIGTASQVSTVEKIVKQRSLHTICEEGRCPNRAECYAQKTA
CCCCCCCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TFLLMGPVCTRACGFCQVAGGKALPLDPQEPEKVAEAVALLGLKYVVLTSVARDDLPDQG
HHEEECHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AGWFVRTMESIRARNCAVEIEVLTPDFRGEEACIATVVAARPVCFNHNIETVRRLQSYAR
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
RAATYARSLAVLAAVKRLDAEIFTKSGLMLGHGETREEVLETLLDLRKVGCDRVTLGQYL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHC
QPSKDHLPVHKYWTPAEFAELGAVARDLGFAHVRSGPLVRSSYHAGEPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure
MLAMSHQTASSRLPEWARRTIGTASQVSTVEKIVKQRSLHTICEEGRCPNRAECYAQKTA
CCCCCCCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TFLLMGPVCTRACGFCQVAGGKALPLDPQEPEKVAEAVALLGLKYVVLTSVARDDLPDQG
HHEEECHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AGWFVRTMESIRARNCAVEIEVLTPDFRGEEACIATVVAARPVCFNHNIETVRRLQSYAR
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
RAATYARSLAVLAAVKRLDAEIFTKSGLMLGHGETREEVLETLLDLRKVGCDRVTLGQYL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHC
QPSKDHLPVHKYWTPAEFAELGAVARDLGFAHVRSGPLVRSSYHAGEPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292