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The map label for this gene is rfbB [H]

Identifier: 37522804

GI number: 37522804

Start: 3442360

End: 3443400

Strand: Direct

Name: rfbB [H]

Synonym: gvip448

Alternate gene names: 37522804

Gene position: 3442360-3443400 (Clockwise)

Preceding gene: 37522803

Following gene: 37522805

Centisome position: 73.89

GC content: 63.5

Gene sequence:

>1041_bases
GTGAAACATCTGTTGGTGACCGGCGGCGCGGGCTTTATCGGCAGCAACTTCGTGCACTACTGGTTTACCCACCACCCGGA
AGATCGGGTCGTCGTGCTCGATAAGCTTACCTATGCCGGCAACCGTGCCAATCTGGAAACTCTCGAAGGCGACCCGCGCC
TGCGCTTTGTGGCGGGGGACATCTGCGATGCGCCGCGGGTCCAGTCGCTGCTGGAGGAGGAGCGCATCGATACGGTGGTG
CACTTTGCCGCCGAATCCCACGTCGATCGCTCGATCGCGGGTCCGGAGCCTTTTATCGAAACCAATATTCGCGGGACGTT
CGTGCTGTTGGAGGCTTGCCGCCGCTACTGGGAGGACGAAGCTTCCGATTGCCGCTTCTTGCACGTCTCGACCGACGAGG
TCTACGGCACCCTGGCCCCGGACGATCCGCCTTTCACCGAGACGACGCCCTATCGGCCCAACAGCCCCTACGCCGCGAGC
AAAGCCGGTTCCGATCACCTGGTGCGCGCCTACTATCACACCTACGGGTTGCCGGTGCTCACCACCAACTGCTCCAACAA
CTACGGCCCTTACCAGTTTCCGGAGAAGTTGATTCCGCTCATCATCACCCGCGCCCTGGAGGGCCAGAGCCTGCCGGTCT
ACGGCGACGGGCAGCAGGTGCGCGACTGGCTCTACGTCGAAGATCACTGCCGGGGGATCAAGGCAGTATTGGAGCGGGGA
CAGCTGGGCGAGACGTACAATATCGGCACCCGCAACGAGCAGCGCAACCTCGAGACGGTCGGACTCGTCTGCGACATCCT
GGGGTCGATTTACCCCGAGCGCGCCGAGCAGTTCCGCTCTCTCATCACCTTCGTCACCGACCGTCCCGGCCACGACCGCC
GCTATGCCATCGACCCCGCGCGCATCGAGCGCGAACTGGATTGGCGACCGGTCGAAAACTTCGAGACGGGCCTGCGCAAA
ACCGTCGAGTGGTACATCGAGAATCGGGCCTGGTGGCAGGCCCTGCGCGCCCGCCAGACGCTGACGTTCCCTTCCAGGTG
A

Upstream 100 bases:

>100_bases
CTCCAACACCCGGGCTCTGCGGGTGCTGGGTGGTCCCCTGCCGCAGTGGCGCCAATCGCTGCGGCGCATGCTCCAACAGC
TCGACAAGGAGGGGTGAATC

Downstream 100 bases:

>100_bases
ACCGTGTCATGTCCACGAGCAGCCCTTTAACCGGTGCCTTTCCCGCCGATGTGCGCTCGGGGTGGTCCTTCCGCCTCAAG
CGTCTTTTCGACGTGCTGCT

Product: dTDP-glucose 4-6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKHLLVTGGAGFIGSNFVHYWFTHHPEDRVVVLDKLTYAGNRANLETLEGDPRLRFVAGDICDAPRVQSLLEEERIDTVV
HFAAESHVDRSIAGPEPFIETNIRGTFVLLEACRRYWEDEASDCRFLHVSTDEVYGTLAPDDPPFTETTPYRPNSPYAAS
KAGSDHLVRAYYHTYGLPVLTTNCSNNYGPYQFPEKLIPLIITRALEGQSLPVYGDGQQVRDWLYVEDHCRGIKAVLERG
QLGETYNIGTRNEQRNLETVGLVCDILGSIYPERAEQFRSLITFVTDRPGHDRRYAIDPARIERELDWRPVENFETGLRK
TVEWYIENRAWWQALRARQTLTFPSR

Sequences:

>Translated_346_residues
MKHLLVTGGAGFIGSNFVHYWFTHHPEDRVVVLDKLTYAGNRANLETLEGDPRLRFVAGDICDAPRVQSLLEEERIDTVV
HFAAESHVDRSIAGPEPFIETNIRGTFVLLEACRRYWEDEASDCRFLHVSTDEVYGTLAPDDPPFTETTPYRPNSPYAAS
KAGSDHLVRAYYHTYGLPVLTTNCSNNYGPYQFPEKLIPLIITRALEGQSLPVYGDGQQVRDWLYVEDHCRGIKAVLERG
QLGETYNIGTRNEQRNLETVGLVCDILGSIYPERAEQFRSLITFVTDRPGHDRRYAIDPARIERELDWRPVENFETGLRK
TVEWYIENRAWWQALRARQTLTFPSR
>Mature_346_residues
MKHLLVTGGAGFIGSNFVHYWFTHHPEDRVVVLDKLTYAGNRANLETLEGDPRLRFVAGDICDAPRVQSLLEEERIDTVV
HFAAESHVDRSIAGPEPFIETNIRGTFVLLEACRRYWEDEASDCRFLHVSTDEVYGTLAPDDPPFTETTPYRPNSPYAAS
KAGSDHLVRAYYHTYGLPVLTTNCSNNYGPYQFPEKLIPLIITRALEGQSLPVYGDGQQVRDWLYVEDHCRGIKAVLERG
QLGETYNIGTRNEQRNLETVGLVCDILGSIYPERAEQFRSLITFVTDRPGHDRRYAIDPARIERELDWRPVENFETGLRK
TVEWYIENRAWWQALRARQTLTFPSR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=39.6396396396396, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=26.2839879154079, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=58.3090379008746, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=56.6473988439306, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=28.1609195402299, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788366, Length=306, Percent_Identity=26.797385620915, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=36.8098159509202, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=35.4354354354354, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=26.2839879154079, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17507723, Length=253, Percent_Identity=26.4822134387352, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=27.355623100304, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=28.4122562674095, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24158427, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39709; Mature: 39709

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLVTGGAGFIGSNFVHYWFTHHPEDRVVVLDKLTYAGNRANLETLEGDPRLRFVAGD
CCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEECCCCCEEEEECC
ICDAPRVQSLLEEERIDTVVHFAAESHVDRSIAGPEPFIETNIRGTFVLLEACRRYWEDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCC
ASDCRFLHVSTDEVYGTLAPDDPPFTETTPYRPNSPYAASKAGSDHLVRAYYHTYGLPVL
CCCCEEEEEEHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
TTNCSNNYGPYQFPEKLIPLIITRALEGQSLPVYGDGQQVRDWLYVEDHCRGIKAVLERG
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHEEHHHHHHHHHHHHHCC
QLGETYNIGTRNEQRNLETVGLVCDILGSIYPERAEQFRSLITFVTDRPGHDRRYAIDPA
CCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECCHH
RIERELDWRPVENFETGLRKTVEWYIENRAWWQALRARQTLTFPSR
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKHLLVTGGAGFIGSNFVHYWFTHHPEDRVVVLDKLTYAGNRANLETLEGDPRLRFVAGD
CCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEECCCCCEEEEECC
ICDAPRVQSLLEEERIDTVVHFAAESHVDRSIAGPEPFIETNIRGTFVLLEACRRYWEDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCC
ASDCRFLHVSTDEVYGTLAPDDPPFTETTPYRPNSPYAASKAGSDHLVRAYYHTYGLPVL
CCCCEEEEEEHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
TTNCSNNYGPYQFPEKLIPLIITRALEGQSLPVYGDGQQVRDWLYVEDHCRGIKAVLERG
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHEEHHHHHHHHHHHHHCC
QLGETYNIGTRNEQRNLETVGLVCDILGSIYPERAEQFRSLITFVTDRPGHDRRYAIDPA
CCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECCHH
RIERELDWRPVENFETGLRKTVEWYIENRAWWQALRARQTLTFPSR
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]