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The map label for this gene is rfbD [H]

Identifier: 37522803

GI number: 37522803

Start: 3441454

End: 3442356

Strand: Direct

Name: rfbD [H]

Synonym: gvip447

Alternate gene names: 37522803

Gene position: 3441454-3442356 (Clockwise)

Preceding gene: 37522801

Following gene: 37522804

Centisome position: 73.87

GC content: 64.23

Gene sequence:

>903_bases
GTGCGCTTTTCCCGACCGATACAGAAGTGTTTTATGTATTTGATTACCGGCGCGGGCGGACAACTGGGCAAGGAGCTGTG
CGCGCTGCTTGAAGCGCGGGAAGAGCCTTTCGTCGCCTATGGGCGGGCACAGCTCGATATCGCCGATTCCGGAGCCGTCC
GGCAGGCCCTTGAGCAGAGCGGTGCAGCGGTGCTTTTCAACTGCGCCGCTTATACCCGCGTCGACAAGGCCGAATCGGAA
GCTGAAGAAGCGCACCGGGTCAACGCCACCGGAGCAAGGGTTCTTGCGGAAGCTTGCAGCGCGGCGGGCAGCCGGTTGGT
CCACGTTTCTACCGACTATGTCTTTGATGGAAGTGCGTGTCGGCCTTACCCGGAAGACGCTCCAACCCAACCGCTCGGGA
TCTACGGCCAGAGTAAGCGCGATGGCGAAGTGGCGGTGCTCGAAGGGGACGGGGAGCATCTGGTGGTCCGCACTGCCTGG
GTCTACGGCGTCGGCGGGCCGAATTTTGTGCGCACGATCTTGCGCCTGGCGGGCGAACGCGAACAGTTGCGCGTGGTGGC
CGATCAGGTGGGCTCTCCTACCTGGACCTTCGACCTGGCCGCAGCGCTCGTGGGATTGACAAAGGCCCAGGCCGAAGGCG
GCATCTACCACGTGACCAATAGCGGGGTGACCAGCTGGTACGACTTCGCGGTCGCAATTGTCGAGGAGGCGCAGGCGCTC
GGTCGTGCCCTCAAGCTGCAAAGCGTGGTACCGATCACCACCGCCGAGTACCCGACACCCGCAGCGCGGCCTGCCTATTC
GGTGCTCTCCAACACCCGGGCTCTGCGGGTGCTGGGTGGTCCCCTGCCGCAGTGGCGCCAATCGCTGCGGCGCATGCTCC
AACAGCTCGACAAGGAGGGGTGA

Upstream 100 bases:

>100_bases
AGGAGGGGGCTCCGGGGCCACGCCCTGTAACGCAGCCAAAAAATCATGGTAAAGTTCTCAACGAGATGTTAAGGGCAGTA
AAGGGCGGGGTGAAGCCACT

Downstream 100 bases:

>100_bases
ATCGTGAAACATCTGTTGGTGACCGGCGGCGCGGGCTTTATCGGCAGCAACTTCGTGCACTACTGGTTTACCCACCACCC
GGAAGATCGGGTCGTCGTGC

Product: dTDP-6-deoxy-L-mannose-dehydrogenase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRFSRPIQKCFMYLITGAGGQLGKELCALLEAREEPFVAYGRAQLDIADSGAVRQALEQSGAAVLFNCAAYTRVDKAESE
AEEAHRVNATGARVLAEACSAAGSRLVHVSTDYVFDGSACRPYPEDAPTQPLGIYGQSKRDGEVAVLEGDGEHLVVRTAW
VYGVGGPNFVRTILRLAGEREQLRVVADQVGSPTWTFDLAAALVGLTKAQAEGGIYHVTNSGVTSWYDFAVAIVEEAQAL
GRALKLQSVVPITTAEYPTPAARPAYSVLSNTRALRVLGGPLPQWRQSLRRMLQQLDKEG

Sequences:

>Translated_300_residues
MRFSRPIQKCFMYLITGAGGQLGKELCALLEAREEPFVAYGRAQLDIADSGAVRQALEQSGAAVLFNCAAYTRVDKAESE
AEEAHRVNATGARVLAEACSAAGSRLVHVSTDYVFDGSACRPYPEDAPTQPLGIYGQSKRDGEVAVLEGDGEHLVVRTAW
VYGVGGPNFVRTILRLAGEREQLRVVADQVGSPTWTFDLAAALVGLTKAQAEGGIYHVTNSGVTSWYDFAVAIVEEAQAL
GRALKLQSVVPITTAEYPTPAARPAYSVLSNTRALRVLGGPLPQWRQSLRRMLQQLDKEG
>Mature_300_residues
MRFSRPIQKCFMYLITGAGGQLGKELCALLEAREEPFVAYGRAQLDIADSGAVRQALEQSGAAVLFNCAAYTRVDKAESE
AEEAHRVNATGARVLAEACSAAGSRLVHVSTDYVFDGSACRPYPEDAPTQPLGIYGQSKRDGEVAVLEGDGEHLVVRTAW
VYGVGGPNFVRTILRLAGEREQLRVVADQVGSPTWTFDLAAALVGLTKAQAEGGIYHVTNSGVTSWYDFAVAIVEEAQAL
GRALKLQSVVPITTAEYPTPAARPAYSVLSNTRALRVLGGPLPQWRQSLRRMLQQLDKEG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=157, Percent_Identity=35.6687898089172, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI33519455, Length=157, Percent_Identity=35.6687898089172, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=39.3835616438356, Blast_Score=193, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32312; Mature: 32312

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSRPIQKCFMYLITGAGGQLGKELCALLEAREEPFVAYGRAQLDIADSGAVRQALEQS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCHHHHHHHHHC
GAAVLFNCAAYTRVDKAESEAEEAHRVNATGARVLAEACSAAGSRLVHVSTDYVFDGSAC
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCCC
RPYPEDAPTQPLGIYGQSKRDGEVAVLEGDGEHLVVRTAWVYGVGGPNFVRTILRLAGER
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHCCH
EQLRVVADQVGSPTWTFDLAAALVGLTKAQAEGGIYHVTNSGVTSWYDFAVAIVEEAQAL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
GRALKLQSVVPITTAEYPTPAARPAYSVLSNTRALRVLGGPLPQWRQSLRRMLQQLDKEG
HHHHHHCCCCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFSRPIQKCFMYLITGAGGQLGKELCALLEAREEPFVAYGRAQLDIADSGAVRQALEQS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCHHHHHHHHHC
GAAVLFNCAAYTRVDKAESEAEEAHRVNATGARVLAEACSAAGSRLVHVSTDYVFDGSAC
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCCC
RPYPEDAPTQPLGIYGQSKRDGEVAVLEGDGEHLVVRTAWVYGVGGPNFVRTILRLAGER
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHCCH
EQLRVVADQVGSPTWTFDLAAALVGLTKAQAEGGIYHVTNSGVTSWYDFAVAIVEEAQAL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
GRALKLQSVVPITTAEYPTPAARPAYSVLSNTRALRVLGGPLPQWRQSLRRMLQQLDKEG
HHHHHHCCCCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]