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The map label for this gene is ndhB

Identifier: 37522689

GI number: 37522689

Start: 3321668

End: 3323209

Strand: Direct

Name: ndhB

Synonym: gvip432

Alternate gene names: 37522689

Gene position: 3321668-3323209 (Clockwise)

Preceding gene: 37522679

Following gene: 37522690

Centisome position: 71.3

GC content: 60.12

Gene sequence:

>1542_bases
ATGAACATCGATCTGGCCGCACTCAACGCCGGGACACTCTGGCCCGAATACATCGTCACCATCACCCTGATTGTGGTGTT
GCTGGTGGATTTGATCTCGGGCCGCAAGGCTTCCTGGTCGCTTCCTTATCTGGCACTTTTGGGGTTGGGAATTGCCACCG
CCACGCTGCTGCCGATGTGGATTCTCGAAAATCCGGTGAGTTTTCTGGGCAGCTTCACCGCCGATCCGCTGAGCGTCGTT
TTTCGCGCCTTTATTTTGATCTCGGCCGCCCTCACGGTGCTGATGTCGGTGCGCTACATCAACCAATCGAGTCTGGCGAC
GGCCGAATTTTATGTGCTGCTATTGGGGGCGACCCTCGGGGCGATGCTGCTGTCGGGCTCCTCGGAGATGGCGATGATCT
TCGTGGCTCTGGAGTTGCTCAGTATCACCAGTTATCTGCTCTCGGGCTACGCCAAGCTCGACAAGCGCTCTAACGAAGCT
TCGCTCAAGTATTTGCTCATCGGTGCGGCCAGTTCCGGTATCTTCCTGTACGGCATGTCGCTGCTGTATGGGTTTTCCGG
CGGCCAGACGCAGCTGACCGAGATCGCCCCGCGCATCGTGAATCTGGGTTTTCCGGCCTTGCTGTCGCTGGTCCTGGTGG
CGGCGGGCATCTGCTTCAAGCTCTCGGCAGTGCCCTTCCACCAGTGGACCCCCGACGTTTACGAAGGATCGCCCACGCCG
GTGGTCGCTTTTTTGTCGGTCGGTTCGAAAGCCGCCGGTTTCGCCCTGGCCATCCGCTTTATGACCTCGGCCTACCCGGG
CTTTTCTGAGCAGTGGCAGACGCTCTTTGTGCTTCTGGCCATCCTCAGTATGGTGCTGGGCAACGTGGTGGCCATCGCCC
AGACCAGCATGAAGCGCATGCTCGCCTACTCGTCGATCGCCCAGGCCGGTTATGTGATGATCGGCCTTGCCATCGGCACC
CAGGAAGGCTACAGCAGCATGATCCTCTACATCGGCACCTACTTGTTTATGAACCTGGGGGCGTTCATGGCGGTGGTGCT
CTTCAGCCTGCGCACCGGCACCGACGAGATCACCGCCTACAGCGGCCTCTACCAGAAAGATCCGTTCCTGACGCTGGTGC
TCAGTTTGTGCCTGCTTTCCTTGGCGGGCATTCCGCCGCTCGCGGGCTTTTTCGGCAAGTTGTACCTGTTTTGGGCGGCG
GTGCAGTCCCAGGCCTATACACTCGTTTTCTTCGGCCTGGTCACCTCGGTGGCATCGATTTATTACTACGTGCGCGTCGT
CAAGCTGATGCTGGTCAAAGAACCGTCTCCCCAGGTGCTCGCTTATTCCGAGGCGGGCGATCAAGACGGCATCCAGACCC
TCAAAGCCGGCATGTTGATTACGACGGTGGCGACCGTCGTGCTGGGAATCTTGTTCCCGCCGCTGATCAGCCTCGCCGAC
ACCTCGCTGCGGGCAACCCCTTCGCTGCAGCAGGTGCGGACCGCAACGCCCGTCTCCCGAGTGAGCACCGGTGCCCAAGC
GCCTGCTGATCACGGGCGCTAG

Upstream 100 bases:

>100_bases
CACATCCCGGCGCCCACGGCGCACAGGGCTCCTGTGGGTCGCCGCCAACGTAAAGTACGATGACAGGTGACAGCTTCCGC
AACAGGCGCAAACTCTGCCC

Downstream 100 bases:

>100_bases
CGGCCTGCTCGGTTTTCAGGTCGCCTCCCGGGCACTGCAAAGCGGTTGGGAAGTGGTGGGTACGGTAAGCCGTCATCGCT
GCCCGCTCCCTATCGAGCAA

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MNIDLAALNAGTLWPEYIVTITLIVVLLVDLISGRKASWSLPYLALLGLGIATATLLPMWILENPVSFLGSFTADPLSVV
FRAFILISAALTVLMSVRYINQSSLATAEFYVLLLGATLGAMLLSGSSEMAMIFVALELLSITSYLLSGYAKLDKRSNEA
SLKYLLIGAASSGIFLYGMSLLYGFSGGQTQLTEIAPRIVNLGFPALLSLVLVAAGICFKLSAVPFHQWTPDVYEGSPTP
VVAFLSVGSKAAGFALAIRFMTSAYPGFSEQWQTLFVLLAILSMVLGNVVAIAQTSMKRMLAYSSIAQAGYVMIGLAIGT
QEGYSSMILYIGTYLFMNLGAFMAVVLFSLRTGTDEITAYSGLYQKDPFLTLVLSLCLLSLAGIPPLAGFFGKLYLFWAA
VQSQAYTLVFFGLVTSVASIYYYVRVVKLMLVKEPSPQVLAYSEAGDQDGIQTLKAGMLITTVATVVLGILFPPLISLAD
TSLRATPSLQQVRTATPVSRVSTGAQAPADHGR

Sequences:

>Translated_513_residues
MNIDLAALNAGTLWPEYIVTITLIVVLLVDLISGRKASWSLPYLALLGLGIATATLLPMWILENPVSFLGSFTADPLSVV
FRAFILISAALTVLMSVRYINQSSLATAEFYVLLLGATLGAMLLSGSSEMAMIFVALELLSITSYLLSGYAKLDKRSNEA
SLKYLLIGAASSGIFLYGMSLLYGFSGGQTQLTEIAPRIVNLGFPALLSLVLVAAGICFKLSAVPFHQWTPDVYEGSPTP
VVAFLSVGSKAAGFALAIRFMTSAYPGFSEQWQTLFVLLAILSMVLGNVVAIAQTSMKRMLAYSSIAQAGYVMIGLAIGT
QEGYSSMILYIGTYLFMNLGAFMAVVLFSLRTGTDEITAYSGLYQKDPFLTLVLSLCLLSLAGIPPLAGFFGKLYLFWAA
VQSQAYTLVFFGLVTSVASIYYYVRVVKLMLVKEPSPQVLAYSEAGDQDGIQTLKAGMLITTVATVVLGILFPPLISLAD
TSLRATPSLQQVRTATPVSRVSTGAQAPADHGR
>Mature_513_residues
MNIDLAALNAGTLWPEYIVTITLIVVLLVDLISGRKASWSLPYLALLGLGIATATLLPMWILENPVSFLGSFTADPLSVV
FRAFILISAALTVLMSVRYINQSSLATAEFYVLLLGATLGAMLLSGSSEMAMIFVALELLSITSYLLSGYAKLDKRSNEA
SLKYLLIGAASSGIFLYGMSLLYGFSGGQTQLTEIAPRIVNLGFPALLSLVLVAAGICFKLSAVPFHQWTPDVYEGSPTP
VVAFLSVGSKAAGFALAIRFMTSAYPGFSEQWQTLFVLLAILSMVLGNVVAIAQTSMKRMLAYSSIAQAGYVMIGLAIGT
QEGYSSMILYIGTYLFMNLGAFMAVVLFSLRTGTDEITAYSGLYQKDPFLTLVLSLCLLSLAGIPPLAGFFGKLYLFWAA
VQSQAYTLVFFGLVTSVASIYYYVRVVKLMLVKEPSPQVLAYSEAGDQDGIQTLKAGMLITTVATVVLGILFPPLISLAD
TSLRATPSLQQVRTATPVSRVSTGAQAPADHGR

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=399, Percent_Identity=26.5664160401003, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI145693160, Length=496, Percent_Identity=34.4758064516129, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788831, Length=441, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788613, Length=383, Percent_Identity=24.2819843342037, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788614, Length=355, Percent_Identity=23.943661971831, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=351, Percent_Identity=26.2108262108262, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788827, Length=282, Percent_Identity=26.241134751773, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU2C_GLOVI (Q7NGP8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926066.1
- GeneID:   2601521
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3120
- HOGENOM:   HBG747830
- OMA:   FAGWADH
- ProtClustDB:   PRK02504
- BioCyc:   GVIO251221:GLR3120-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.6.5.3

Molecular weight: Translated: 54858; Mature: 54858

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x169ffcb0)-; HASH(0x15eabc7c)-; HASH(0x15a127ac)-; HASH(0x163db004)-; HASH(0x16d4127c)-; HASH(0x16d64dd0)-; HASH(0x14dfa3d4)-; HASH(0x15fca3b0)-; HASH(0x16bf70b8)-; HASH(0x15829e2c)-; HASH(0x169109b8)-; HASH(0x1619257c)-; HASH(0x16786c04)-; HASH(0x15d0def4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDLAALNAGTLWPEYIVTITLIVVLLVDLISGRKASWSLPYLALLGLGIATATLLPMW
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILENPVSFLGSFTADPLSVVFRAFILISAALTVLMSVRYINQSSLATAEFYVLLLGATLG
HHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AMLLSGSSEMAMIFVALELLSITSYLLSGYAKLDKRSNEASLKYLLIGAASSGIFLYGMS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LLYGFSGGQTQLTEIAPRIVNLGFPALLSLVLVAAGICFKLSAVPFHQWTPDVYEGSPTP
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VVAFLSVGSKAAGFALAIRFMTSAYPGFSEQWQTLFVLLAILSMVLGNVVAIAQTSMKRM
EEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYSSIAQAGYVMIGLAIGTQEGYSSMILYIGTYLFMNLGAFMAVVLFSLRTGTDEITAY
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SGLYQKDPFLTLVLSLCLLSLAGIPPLAGFFGKLYLFWAAVQSQAYTLVFFGLVTSVASI
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
YYYVRVVKLMLVKEPSPQVLAYSEAGDQDGIQTLKAGMLITTVATVVLGILFPPLISLAD
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TSLRATPSLQQVRTATPVSRVSTGAQAPADHGR
CCCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCH
>Mature Secondary Structure
MNIDLAALNAGTLWPEYIVTITLIVVLLVDLISGRKASWSLPYLALLGLGIATATLLPMW
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILENPVSFLGSFTADPLSVVFRAFILISAALTVLMSVRYINQSSLATAEFYVLLLGATLG
HHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AMLLSGSSEMAMIFVALELLSITSYLLSGYAKLDKRSNEASLKYLLIGAASSGIFLYGMS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LLYGFSGGQTQLTEIAPRIVNLGFPALLSLVLVAAGICFKLSAVPFHQWTPDVYEGSPTP
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VVAFLSVGSKAAGFALAIRFMTSAYPGFSEQWQTLFVLLAILSMVLGNVVAIAQTSMKRM
EEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYSSIAQAGYVMIGLAIGTQEGYSSMILYIGTYLFMNLGAFMAVVLFSLRTGTDEITAY
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SGLYQKDPFLTLVLSLCLLSLAGIPPLAGFFGKLYLFWAAVQSQAYTLVFFGLVTSVASI
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
YYYVRVVKLMLVKEPSPQVLAYSEAGDQDGIQTLKAGMLITTVATVVLGILFPPLISLAD
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TSLRATPSLQQVRTATPVSRVSTGAQAPADHGR
CCCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14621292