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The map label for this gene is spsK [H]

Identifier: 37522690

GI number: 37522690

Start: 3323178

End: 3324065

Strand: Direct

Name: spsK [H]

Synonym: glr3121

Alternate gene names: 37522690

Gene position: 3323178-3324065 (Clockwise)

Preceding gene: 37522689

Following gene: 37522693

Centisome position: 71.33

GC content: 67.57

Gene sequence:

>888_bases
GTGCCCAAGCGCCTGCTGATCACGGGCGCTAGCGGCCTGCTCGGTTTTCAGGTCGCCTCCCGGGCACTGCAAAGCGGTTG
GGAAGTGGTGGGTACGGTAAGCCGTCATCGCTGCCCGCTCCCTATCGAGCAACCGCAGTTGGACCTGGCCTTGGAGGATC
TTTCGGAGCTTGAAGCTTTTGTCGGACGGTACCGCCCCGACGCCCTCGTCCATTGCGCGGCGATTAGCGAAGCGGCCCAG
TGCGAAGCCAATCCCACCGTGGCGTTGCGCGTCAATGTCGCAGCGACCGAAGCGCTCGCCCGCACGGCCGGCCGCACAGG
CTGCCGCTTCGTGTTCGTCTCCACCGACCTGGTCTTCGACGGCCGGGAGGCTCCCTACTGCGAGGAAAGCCTCCCCAGCC
CACTCGGCTGCTACGGCCGCACCAAGATGGCGGCTGAGCAGCGAGTGCTGGAACTGGGGGACGGGGGGGCGTTGGTCGTT
CGCACTTCGCTGCTTTTGGGACCAAGCCCGTCGGGCGCGCGCAGCGTCGAGGAGCGCCTGGGAGCCCAACTGGCCGCCGG
AAAACGGGCGAACTTGTTCACAGACGAATTTCGCTCGCCGGTGTACGCCCCGGATCTGGCTGCGGCCCTGCTCGAACTGG
TGGAGGCGGGACAATCCGGGCTGCTGCACCTGGGCGGGCCTGAGCGCCTATCGCGCCACGCCCTGGGGATACTGCTGGCG
GGCCACTTCGGCTGGGACACGCGTTTGATCCTGGCCGCCTCGGGTAGAGACTATCCTTCGACCCCGCCCCGGCCGACCGA
TGTCAGCCTCGATTCGCGGCGGGCCTACGCGCTGCTCTCTTCTCCCCCCCGGCCCCTTGGCCGGGTGTTTGCTCCCCGAA
ACCAATAA

Upstream 100 bases:

>100_bases
TTGTTCCCGCCGCTGATCAGCCTCGCCGACACCTCGCTGCGGGCAACCCCTTCGCTGCAGCAGGTGCGGACCGCAACGCC
CGTCTCCCGAGTGAGCACCG

Downstream 100 bases:

>100_bases
GCGATCACTTCGCGGCCGGTAACGTCACCGTGAAGGTACTGCCTTTGCCGGGAGCACTGGTGAGACTGAGGGTGCCGCCG
TGGGCTTCGACAATCTGGCG

Product: methionine adenosyltransferase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPKRLLITGASGLLGFQVASRALQSGWEVVGTVSRHRCPLPIEQPQLDLALEDLSELEAFVGRYRPDALVHCAAISEAAQ
CEANPTVALRVNVAATEALARTAGRTGCRFVFVSTDLVFDGREAPYCEESLPSPLGCYGRTKMAAEQRVLELGDGGALVV
RTSLLLGPSPSGARSVEERLGAQLAAGKRANLFTDEFRSPVYAPDLAAALLELVEAGQSGLLHLGGPERLSRHALGILLA
GHFGWDTRLILAASGRDYPSTPPRPTDVSLDSRRAYALLSSPPRPLGRVFAPRNQ

Sequences:

>Translated_295_residues
MPKRLLITGASGLLGFQVASRALQSGWEVVGTVSRHRCPLPIEQPQLDLALEDLSELEAFVGRYRPDALVHCAAISEAAQ
CEANPTVALRVNVAATEALARTAGRTGCRFVFVSTDLVFDGREAPYCEESLPSPLGCYGRTKMAAEQRVLELGDGGALVV
RTSLLLGPSPSGARSVEERLGAQLAAGKRANLFTDEFRSPVYAPDLAAALLELVEAGQSGLLHLGGPERLSRHALGILLA
GHFGWDTRLILAASGRDYPSTPPRPTDVSLDSRRAYALLSSPPRPLGRVFAPRNQ
>Mature_294_residues
PKRLLITGASGLLGFQVASRALQSGWEVVGTVSRHRCPLPIEQPQLDLALEDLSELEAFVGRYRPDALVHCAAISEAAQC
EANPTVALRVNVAATEALARTAGRTGCRFVFVSTDLVFDGREAPYCEESLPSPLGCYGRTKMAAEQRVLELGDGGALVVR
TSLLLGPSPSGARSVEERLGAQLAAGKRANLFTDEFRSPVYAPDLAAALLELVEAGQSGLLHLGGPERLSRHALGILLAG
HFGWDTRLILAASGRDYPSTPPRPTDVSLDSRRAYALLSSPPRPLGRVFAPRNQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=249, Percent_Identity=30.9236947791165, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI11034825, Length=249, Percent_Identity=30.9236947791165, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788352, Length=170, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 31485; Mature: 31354

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRLLITGASGLLGFQVASRALQSGWEVVGTVSRHRCPLPIEQPQLDLALEDLSELEAF
CCCEEEEECCCCHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VGRYRPDALVHCAAISEAAQCEANPTVALRVNVAATEALARTAGRTGCRFVFVSTDLVFD
HHCCCCCHHEEHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEEEEEEEEEC
GREAPYCEESLPSPLGCYGRTKMAAEQRVLELGDGGALVVRTSLLLGPSPSGARSVEERL
CCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHH
GAQLAAGKRANLFTDEFRSPVYAPDLAAALLELVEAGQSGLLHLGGPERLSRHALGILLA
HHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHEEEEE
GHFGWDTRLILAASGRDYPSTPPRPTDVSLDSRRAYALLSSPPRPLGRVFAPRNQ
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PKRLLITGASGLLGFQVASRALQSGWEVVGTVSRHRCPLPIEQPQLDLALEDLSELEAF
CCEEEEECCCCHHHHHHHHHHHHCCHHEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VGRYRPDALVHCAAISEAAQCEANPTVALRVNVAATEALARTAGRTGCRFVFVSTDLVFD
HHCCCCCHHEEHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEEEEEEEEEC
GREAPYCEESLPSPLGCYGRTKMAAEQRVLELGDGGALVVRTSLLLGPSPSGARSVEERL
CCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHH
GAQLAAGKRANLFTDEFRSPVYAPDLAAALLELVEAGQSGLLHLGGPERLSRHALGILLA
HHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHEEEEE
GHFGWDTRLILAASGRDYPSTPPRPTDVSLDSRRAYALLSSPPRPLGRVFAPRNQ
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]