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The map label for this gene is moaA

Identifier: 37522593

GI number: 37522593

Start: 3222128

End: 3223105

Strand: Reverse

Name: moaA

Synonym: gll3024

Alternate gene names: 37522593

Gene position: 3223105-3222128 (Counterclockwise)

Preceding gene: 37522594

Following gene: 37522592

Centisome position: 69.18

GC content: 62.07

Gene sequence:

>978_bases
ATGAACACCGTCAACTACCTGCGCATTTCCCTGGTGGATCGCTGCAACTTTCGCTGCAGCTACTGCATGCCCACGGGCGA
AGCGATAGCCTACCTGCACCGCTCGCAGATCTTGAGCTACGAGGAGCTGCTGTTTCTGGTGGGCGAAGTCTTTTTGCCCC
TGGGGATCGACCGCTTTCGTCTCACCGGCGGCGAGCCGTTGCTCAGGCGCGGGGTAGTGGGCTTTGTGCGCGCCCTGGTG
GGATTGCCTGGGGTGGCGGACGTGTCGCTGAGCACCAACGCCTACCTGCTCGAAGAACTGGCGGAGGACCTGTACGCCGC
CGGTCTGAGGCGGGTGAATATTTCACTGGATTCCCTCGACCCAGGGGTATTCGCGCGCATCACCGGCCGCGACCACTGGT
CCAAAGTCTGGGCGGGCATCCAGGCGGCCCACCGGGTCGGTTTCGACCCCCTCAAGCTCAATGTCGTGGTCCTGCCGGGG
ATCAACGAACAGGAAGTCCCGGATCTGGCTGCCCTGACCATCGATCGGCAATGGCACGTGCGCTTTATCGAATTTATGCC
GATCGGCAACACCGGTTACTTTGAGCAGGCAGGCTGGGTGAATTCCGAGACGCTGCGAAGCCGTATCCGCGAGCGCTTCG
GCTTGGGAGAGGGTGCCTGCTACGGCAACGGCCCGGCCGATGTGTTTCAGATTCCAGGTGCCAAAGGCACCGTCGGCTTT
ATCTCCCAGATGTCCGAATGCTTTTGCAGCCGTTGCAACCGGGTGCGACTGGCGGCCGACGGTTTTTTGCGGCCCTGTCT
GCTCAACGAAGCCGATCAAATCGACCTCAAGACGCCCTTGCGCGCCGGGGCGGGCGCGGCCCAGCTGCGCGAACTGGTAA
GTGAACTGCTGCTTCGCAAACCCGAAATCAACTTTCGCGAGCGCGACCGGGGGACCACCGGCTCCTATGGCCGCACCATG
TCCCAAATTGGTGGTTAA

Upstream 100 bases:

>100_bases
TAGGCGAAGGGTTCCTAGCCGAGATCCTGGGCAACTACGTTGCCGAAGTCGGCAACCCCAGTCTCTAGGCACCCTTGATT
TCTCCGCATTTGGACTGGTC

Downstream 100 bases:

>100_bases
TACCTATTGCCCACAGCCCTGGCGCCCGATATCGGGGCTTTGGGCTGTGGGCCACCACCGACGGGGGGAGACGGGCCGCT
ATGGGCCGGGGGCAATAAAC

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNTVNYLRISLVDRCNFRCSYCMPTGEAIAYLHRSQILSYEELLFLVGEVFLPLGIDRFRLTGGEPLLRRGVVGFVRALV
GLPGVADVSLSTNAYLLEELAEDLYAAGLRRVNISLDSLDPGVFARITGRDHWSKVWAGIQAAHRVGFDPLKLNVVVLPG
INEQEVPDLAALTIDRQWHVRFIEFMPIGNTGYFEQAGWVNSETLRSRIRERFGLGEGACYGNGPADVFQIPGAKGTVGF
ISQMSECFCSRCNRVRLAADGFLRPCLLNEADQIDLKTPLRAGAGAAQLRELVSELLLRKPEINFRERDRGTTGSYGRTM
SQIGG

Sequences:

>Translated_325_residues
MNTVNYLRISLVDRCNFRCSYCMPTGEAIAYLHRSQILSYEELLFLVGEVFLPLGIDRFRLTGGEPLLRRGVVGFVRALV
GLPGVADVSLSTNAYLLEELAEDLYAAGLRRVNISLDSLDPGVFARITGRDHWSKVWAGIQAAHRVGFDPLKLNVVVLPG
INEQEVPDLAALTIDRQWHVRFIEFMPIGNTGYFEQAGWVNSETLRSRIRERFGLGEGACYGNGPADVFQIPGAKGTVGF
ISQMSECFCSRCNRVRLAADGFLRPCLLNEADQIDLKTPLRAGAGAAQLRELVSELLLRKPEINFRERDRGTTGSYGRTM
SQIGG
>Mature_325_residues
MNTVNYLRISLVDRCNFRCSYCMPTGEAIAYLHRSQILSYEELLFLVGEVFLPLGIDRFRLTGGEPLLRRGVVGFVRALV
GLPGVADVSLSTNAYLLEELAEDLYAAGLRRVNISLDSLDPGVFARITGRDHWSKVWAGIQAAHRVGFDPLKLNVVVLPG
INEQEVPDLAALTIDRQWHVRFIEFMPIGNTGYFEQAGWVNSETLRSRIRERFGLGEGACYGNGPADVFQIPGAKGTVGF
ISQMSECFCSRCNRVRLAADGFLRPCLLNEADQIDLKTPLRAGAGAAQLRELVSELLLRKPEINFRERDRGTTGSYGRTM
SQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=298, Percent_Identity=40.9395973154362, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI22027536, Length=298, Percent_Identity=40.9395973154362, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786999, Length=321, Percent_Identity=32.7102803738318, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI32566331, Length=303, Percent_Identity=40.2640264026403, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI32566329, Length=303, Percent_Identity=40.2640264026403, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17561210, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI28574490, Length=302, Percent_Identity=38.4105960264901, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI28574488, Length=301, Percent_Identity=38.5382059800665, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_GLOVI (Q7NCF5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925970.1
- ProteinModelPortal:   Q7NCF5
- SMR:   Q7NCF5
- GeneID:   2601425
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3024
- NMPDR:   fig|251221.1.peg.3024
- HOGENOM:   HBG529885
- OMA:   TEDPCFL
- ProtClustDB:   CLSK896062
- BioCyc:   GVIO251221:GLL3024-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36002; Mature: 36002

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 8-8 BINDING 21-21 BINDING 60-60 BINDING 64-64 BINDING 91-91 BINDING 115-115 BINDING 152-152 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVNYLRISLVDRCNFRCSYCMPTGEAIAYLHRSQILSYEELLFLVGEVFLPLGIDRFR
CCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEE
LTGGEPLLRRGVVGFVRALVGLPGVADVSLSTNAYLLEELAEDLYAAGLRRVNISLDSLD
ECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCC
PGVFARITGRDHWSKVWAGIQAAHRVGFDPLKLNVVVLPGINEQEVPDLAALTIDRQWHV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCEEE
RFIEFMPIGNTGYFEQAGWVNSETLRSRIRERFGLGEGACYGNGPADVFQIPGAKGTVGF
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHH
ISQMSECFCSRCNRVRLAADGFLRPCLLNEADQIDLKTPLRAGAGAAQLRELVSELLLRK
HHHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
PEINFRERDRGTTGSYGRTMSQIGG
CCCCCHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNTVNYLRISLVDRCNFRCSYCMPTGEAIAYLHRSQILSYEELLFLVGEVFLPLGIDRFR
CCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEE
LTGGEPLLRRGVVGFVRALVGLPGVADVSLSTNAYLLEELAEDLYAAGLRRVNISLDSLD
ECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCC
PGVFARITGRDHWSKVWAGIQAAHRVGFDPLKLNVVVLPGINEQEVPDLAALTIDRQWHV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCEEE
RFIEFMPIGNTGYFEQAGWVNSETLRSRIRERFGLGEGACYGNGPADVFQIPGAKGTVGF
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHH
ISQMSECFCSRCNRVRLAADGFLRPCLLNEADQIDLKTPLRAGAGAAQLRELVSELLLRK
HHHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
PEINFRERDRGTTGSYGRTMSQIGG
CCCCCHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292