Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 37522592

GI number: 37522592

Start: 3220371

End: 3221921

Strand: Reverse

Name: metG [H]

Synonym: gvip415

Alternate gene names: 37522592

Gene position: 3221921-3220371 (Counterclockwise)

Preceding gene: 37522593

Following gene: 37522591

Centisome position: 69.15

GC content: 59.83

Gene sequence:

>1551_bases
ATGACGCAGGGTTTTGCACTTACAACCCCACTATTTTACGTCAATGCTTTGCCCCACATCGGCAGTGCCTACCCGACGCT
GGCGGCGGACGCGGTGGCGCGCTATCACCGCTTGCGGGGTCGCTCCGTGCGTTTTGTGACCGGCACCGACGAGTACGGGC
TCAAAATCGAGCGGCAGGCGGCGGAGCGTCATTTGACTCCCCGTGAGCACTGCAACGAGATTGCCGCCGGGTTCGAGAAG
CTGTGGCGGGCGCTGTCGATCGATTACGACCGCTTTATCCGCACCACCGATCCGCGCCACGCTGCGATCGTACGCGAATT
TTTTGAGCGCTGCTGGCAGGCGGGCGACATTTACAAAGGCCGCTACACGGGCCTCTACTGCATCGATTGCGAAGAATTCA
AAAAAAAGCCCGACGAGGACTACTTTATCGAGAACGGCGAGCCGCGCTGCAAGATTCACCTCAAGCCGCTGCGCGAGCAG
GACGAAGAGAACTATATCTTTGCGCTCTCGCGCTACCAGCAAAAACTGGAACAATACTTCGACGAGCATCCCGAGTTTGT
GCAGCCCGATTTTCGTGCCCACGAGGTGCGCAATTTCATTGCCGGGGGACTCGAAGATTTTTCGATCTCGCGGGCCAACG
TCTCCTGGGGGCTGCCGATTCCCGTCGATTCCAGCCAGACGATTTATGTCTGGTTCAACGCGCTGTTGGGCTATGTGACG
GCCCTGCTCGAACCCGAGGACGAACCCACCTTAGAAAATGCCCTCGCGCGCTGGTGGCCCATCGATCTGCACATTGTCGG
GAAGGACATTTTGCGCTTTCACGCCGTCTACTGGCCGGCGATGCTGATGTCGGCGGGGTTGCCGCTGCCGGGGATGATCT
TCGGCCACGGGTTTTTGACGCGCGACGGCCGCAAGATGGGCAAGGCCCTCGGCAACGTCATCGACCCGGGTGTATTGGTT
GAACAGTATGGCTCCGATGCGGTCCGTTACTATTTTCTCAAAGCGATCGAATTCGGACGGGACAACGACTTTAACGAGAC
CCGTTTTCGCGAGATTCTCAACGCAGATCTGGCCAATGATCTGGGCAATTTGCTGAACCGAACTGGTAATATGGTCGTCA
AATATTGCGGTGGTGTTATCCCTTCCGTTGCCATCGATCCCACCGACGCCCTGCGGGACAAGGCCACCGGCCTCGCGCAG
GTGTGCGCCGAGCATTGGGAGGCGCTGCGGTTTTCCGAGGCAAGCGGGTTGGCGTTGGCCCTGGTGCGGGCGGGCAACCG
CTACCTCGATGCGCGCGCCCCCTGGTCGCTGTACAAAAAGGGCGAGCAGGTGCAGGTTGAACAGGTACTCTACGCGGTTC
TCGAATCGGTGCGCATCGCAGCGGTATTGCTGTCGCCGCTGATTCCGGGGTTGGCCGCCGAGATCTACCGGCAACTGGGA
TTCTCCGTCCAGGCTTGGGAGGATCGCAGTTGGGACGATGCCTTGTGGGGCGGTTTGCCGGGGGGGCAGCCGCTCTGTTT
GGGGTCGCCTCTATTTCCCCGCCTGGAATAG

Upstream 100 bases:

>100_bases
TGTATGATTGCCGTACCTCCCGAACTTTTTCTGTTATAATTCGTTAACATTTTTCGGTTTTCGGAACGGTTCATTCCAGT
TCGATTGAGTCACAAGTCAG

Downstream 100 bases:

>100_bases
GGTTCATTTATCTCCGACGAGGTTGACTACTTTATGACCGCTTCTTCAGATACCGAAGGATTACTAGCCCGAGAGCAGGC
GATGATGCGCGGGCGGGTGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTQGFALTTPLFYVNALPHIGSAYPTLAADAVARYHRLRGRSVRFVTGTDEYGLKIERQAAERHLTPREHCNEIAAGFEK
LWRALSIDYDRFIRTTDPRHAAIVREFFERCWQAGDIYKGRYTGLYCIDCEEFKKKPDEDYFIENGEPRCKIHLKPLREQ
DEENYIFALSRYQQKLEQYFDEHPEFVQPDFRAHEVRNFIAGGLEDFSISRANVSWGLPIPVDSSQTIYVWFNALLGYVT
ALLEPEDEPTLENALARWWPIDLHIVGKDILRFHAVYWPAMLMSAGLPLPGMIFGHGFLTRDGRKMGKALGNVIDPGVLV
EQYGSDAVRYYFLKAIEFGRDNDFNETRFREILNADLANDLGNLLNRTGNMVVKYCGGVIPSVAIDPTDALRDKATGLAQ
VCAEHWEALRFSEASGLALALVRAGNRYLDARAPWSLYKKGEQVQVEQVLYAVLESVRIAAVLLSPLIPGLAAEIYRQLG
FSVQAWEDRSWDDALWGGLPGGQPLCLGSPLFPRLE

Sequences:

>Translated_516_residues
MTQGFALTTPLFYVNALPHIGSAYPTLAADAVARYHRLRGRSVRFVTGTDEYGLKIERQAAERHLTPREHCNEIAAGFEK
LWRALSIDYDRFIRTTDPRHAAIVREFFERCWQAGDIYKGRYTGLYCIDCEEFKKKPDEDYFIENGEPRCKIHLKPLREQ
DEENYIFALSRYQQKLEQYFDEHPEFVQPDFRAHEVRNFIAGGLEDFSISRANVSWGLPIPVDSSQTIYVWFNALLGYVT
ALLEPEDEPTLENALARWWPIDLHIVGKDILRFHAVYWPAMLMSAGLPLPGMIFGHGFLTRDGRKMGKALGNVIDPGVLV
EQYGSDAVRYYFLKAIEFGRDNDFNETRFREILNADLANDLGNLLNRTGNMVVKYCGGVIPSVAIDPTDALRDKATGLAQ
VCAEHWEALRFSEASGLALALVRAGNRYLDARAPWSLYKKGEQVQVEQVLYAVLESVRIAAVLLSPLIPGLAAEIYRQLG
FSVQAWEDRSWDDALWGGLPGGQPLCLGSPLFPRLE
>Mature_515_residues
TQGFALTTPLFYVNALPHIGSAYPTLAADAVARYHRLRGRSVRFVTGTDEYGLKIERQAAERHLTPREHCNEIAAGFEKL
WRALSIDYDRFIRTTDPRHAAIVREFFERCWQAGDIYKGRYTGLYCIDCEEFKKKPDEDYFIENGEPRCKIHLKPLREQD
EENYIFALSRYQQKLEQYFDEHPEFVQPDFRAHEVRNFIAGGLEDFSISRANVSWGLPIPVDSSQTIYVWFNALLGYVTA
LLEPEDEPTLENALARWWPIDLHIVGKDILRFHAVYWPAMLMSAGLPLPGMIFGHGFLTRDGRKMGKALGNVIDPGVLVE
QYGSDAVRYYFLKAIEFGRDNDFNETRFREILNADLANDLGNLLNRTGNMVVKYCGGVIPSVAIDPTDALRDKATGLAQV
CAEHWEALRFSEASGLALALVRAGNRYLDARAPWSLYKKGEQVQVEQVLYAVLESVRIAAVLLSPLIPGLAAEIYRQLGF
SVQAWEDRSWDDALWGGLPGGQPLCLGSPLFPRLE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=512, Percent_Identity=37.109375, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=27.5179856115108, Blast_Score=185, Evalue=9e-47,
Organism=Escherichia coli, GI1788432, Length=497, Percent_Identity=26.3581488933602, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=31.7738791423002, Blast_Score=281, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI71992781, Length=403, Percent_Identity=30.0248138957816, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=558, Percent_Identity=27.4193548387097, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321610, Length=526, Percent_Identity=30.7984790874525, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6321704, Length=528, Percent_Identity=22.7272727272727, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21358483, Length=500, Percent_Identity=33.8, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=26.2032085561497, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58549; Mature: 58418

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGFALTTPLFYVNALPHIGSAYPTLAADAVARYHRLRGRSVRFVTGTDEYGLKIERQA
CCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHH
AERHLTPREHCNEIAAGFEKLWRALSIDYDRFIRTTDPRHAAIVREFFERCWQAGDIYKG
HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
RYTGLYCIDCEEFKKKPDEDYFIENGEPRCKIHLKPLREQDEENYIFALSRYQQKLEQYF
CCCCEEEEEHHHHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
DEHPEFVQPDFRAHEVRNFIAGGLEDFSISRANVSWGLPIPVDSSQTIYVWFNALLGYVT
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
ALLEPEDEPTLENALARWWPIDLHIVGKDILRFHAVYWPAMLMSAGLPLPGMIFGHGFLT
HHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
RDGRKMGKALGNVIDPGVLVEQYGSDAVRYYFLKAIEFGRDNDFNETRFREILNADLAND
CCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGNLLNRTGNMVVKYCGGVIPSVAIDPTDALRDKATGLAQVCAEHWEALRFSEASGLALA
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
LVRAGNRYLDARAPWSLYKKGEQVQVEQVLYAVLESVRIAAVLLSPLIPGLAAEIYRQLG
EECCCCCEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
FSVQAWEDRSWDDALWGGLPGGQPLCLGSPLFPRLE
CCEEECCCCCCCHHHHCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TQGFALTTPLFYVNALPHIGSAYPTLAADAVARYHRLRGRSVRFVTGTDEYGLKIERQA
CCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHH
AERHLTPREHCNEIAAGFEKLWRALSIDYDRFIRTTDPRHAAIVREFFERCWQAGDIYKG
HHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
RYTGLYCIDCEEFKKKPDEDYFIENGEPRCKIHLKPLREQDEENYIFALSRYQQKLEQYF
CCCCEEEEEHHHHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
DEHPEFVQPDFRAHEVRNFIAGGLEDFSISRANVSWGLPIPVDSSQTIYVWFNALLGYVT
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
ALLEPEDEPTLENALARWWPIDLHIVGKDILRFHAVYWPAMLMSAGLPLPGMIFGHGFLT
HHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
RDGRKMGKALGNVIDPGVLVEQYGSDAVRYYFLKAIEFGRDNDFNETRFREILNADLAND
CCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LGNLLNRTGNMVVKYCGGVIPSVAIDPTDALRDKATGLAQVCAEHWEALRFSEASGLALA
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
LVRAGNRYLDARAPWSLYKKGEQVQVEQVLYAVLESVRIAAVLLSPLIPGLAAEIYRQLG
EECCCCCEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
FSVQAWEDRSWDDALWGGLPGGQPLCLGSPLFPRLE
CCEEECCCCCCCHHHHCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]