Click here to switch to the map view.

The map label for this gene is azoB [H]

Identifier: 37522460

GI number: 37522460

Start: 3085034

End: 3085888

Strand: Reverse

Name: azoB [H]

Synonym: gll2891

Alternate gene names: 37522460

Gene position: 3085888-3085034 (Counterclockwise)

Preceding gene: 37522461

Following gene: 37522459

Centisome position: 66.23

GC content: 65.85

Gene sequence:

>855_bases
ATGATCTTGATTACCGGCGCCACCGGCAACAACGGGGTGGAAATCGTCCGATTGCTTGCGCAGAAAAACATCTCAGCGCG
CGCACTGGTGCGCGACTCCAAGCGGGCCGGCGCTGTCGCGTTGCCCCACATCGAGGTTGTCGAGGGGAATTTCGACCGCC
CCGAGACTCTCCTTGCCGCCCTCGTCGGCGTCGATCGGGCGTTCCTGCTCACCCCTTCCTCCGAACGCGCCGAAAGCCAA
CAACTCGCCTTCATCGACGCGGCCCGGCAAATCGGTATCAGGCACATCGTGAAATTGTCGCAGTTTGGTGCCGACACCCG
CTCTGAGGGCCGCTTCCAGCGCTACCATGCGGTGGTGGAGGCGGCCGTGCGGGCGTCCGGCCTGGCCTGCACGTTCCTAA
GACCCAACCTCTTTATGCAGGGACTGCTCAATTTCCGCGCGGTTATCGCCGCCCGCCAGGCCTTCTACGCGGCGGCCGGC
GACGCGAAAGTCAGCATCGTCGACGTGCGCGACGTCGCAGAGGTCGCCGTGGCCGCCCTGACGGAGAGCGGCCACGAAGG
GAAAACCTACGAACTGACCGGCCCCGAAGCACTCACTCACACCGAGATGGCAGAACACCTCTCCGCCTCGCTGGGGCACC
ACGTCGCCTTTGTCGACATCCCGCCCGAAGCCATGGGCGAGGCCCTGCGCGGTGCCGGGCTCCCACCCTGGCAGGCGGAG
GGATTGATCGAAGAGTACGCGCTCTACCGGCGCGGTGAGGCCGAGGCGATCACCTCCGGCGTCCAGGACGCGATTGGCAG
AACGCCGCGCAGTTTCAATGCGTTCGCGCGCGACTACGCGGCGGCGTTTTCCTGA

Upstream 100 bases:

>100_bases
GCGTGAACAAACAAGTGAGGACCCGAAACGAGGGGAACGCGTCGCTGCTCGAGTGTGGCTGCGCTGAAAGCTAACAGAAC
CCAGTCGAGGAGGAGACCCA

Downstream 100 bases:

>100_bases
TGGGCAAGGCCGCCCGGATTCTGCCAGGCGCATTTTTAGGTTCTGTTGACGGATGAGAAGAAAGAGCTTTAATTTCAATA
GGAATTAATGATTGTCATGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MILITGATGNNGVEIVRLLAQKNISARALVRDSKRAGAVALPHIEVVEGNFDRPETLLAALVGVDRAFLLTPSSERAESQ
QLAFIDAARQIGIRHIVKLSQFGADTRSEGRFQRYHAVVEAAVRASGLACTFLRPNLFMQGLLNFRAVIAARQAFYAAAG
DAKVSIVDVRDVAEVAVAALTESGHEGKTYELTGPEALTHTEMAEHLSASLGHHVAFVDIPPEAMGEALRGAGLPPWQAE
GLIEEYALYRRGEAEAITSGVQDAIGRTPRSFNAFARDYAAAFS

Sequences:

>Translated_284_residues
MILITGATGNNGVEIVRLLAQKNISARALVRDSKRAGAVALPHIEVVEGNFDRPETLLAALVGVDRAFLLTPSSERAESQ
QLAFIDAARQIGIRHIVKLSQFGADTRSEGRFQRYHAVVEAAVRASGLACTFLRPNLFMQGLLNFRAVIAARQAFYAAAG
DAKVSIVDVRDVAEVAVAALTESGHEGKTYELTGPEALTHTEMAEHLSASLGHHVAFVDIPPEAMGEALRGAGLPPWQAE
GLIEEYALYRRGEAEAITSGVQDAIGRTPRSFNAFARDYAAAFS
>Mature_284_residues
MILITGATGNNGVEIVRLLAQKNISARALVRDSKRAGAVALPHIEVVEGNFDRPETLLAALVGVDRAFLLTPSSERAESQ
QLAFIDAARQIGIRHIVKLSQFGADTRSEGRFQRYHAVVEAAVRASGLACTFLRPNLFMQGLLNFRAVIAARQAFYAAAG
DAKVSIVDVRDVAEVAVAALTESGHEGKTYELTGPEALTHTEMAEHLSASLGHHVAFVDIPPEAMGEALRGAGLPPWQAE
GLIEEYALYRRGEAEAITSGVQDAIGRTPRSFNAFARDYAAAFS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=249, Percent_Identity=32.9317269076305, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 30449; Mature: 30449

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGATGNNGVEIVRLLAQKNISARALVRDSKRAGAVALPHIEVVEGNFDRPETLLAA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCEEEEECCCCCCHHHHHHH
LVGVDRAFLLTPSSERAESQQLAFIDAARQIGIRHIVKLSQFGADTRSEGRFQRYHAVVE
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AAVRASGLACTFLRPNLFMQGLLNFRAVIAARQAFYAAAGDAKVSIVDVRDVAEVAVAAL
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
TESGHEGKTYELTGPEALTHTEMAEHLSASLGHHVAFVDIPPEAMGEALRGAGLPPWQAE
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCHH
GLIEEYALYRRGEAEAITSGVQDAIGRTPRSFNAFARDYAAAFS
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MILITGATGNNGVEIVRLLAQKNISARALVRDSKRAGAVALPHIEVVEGNFDRPETLLAA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCEEEEECCCCCCHHHHHHH
LVGVDRAFLLTPSSERAESQQLAFIDAARQIGIRHIVKLSQFGADTRSEGRFQRYHAVVE
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AAVRASGLACTFLRPNLFMQGLLNFRAVIAARQAFYAAAGDAKVSIVDVRDVAEVAVAAL
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
TESGHEGKTYELTGPEALTHTEMAEHLSASLGHHVAFVDIPPEAMGEALRGAGLPPWQAE
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCHH
GLIEEYALYRRGEAEAITSGVQDAIGRTPRSFNAFARDYAAAFS
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]