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The map label for this gene is mjaIIIM [H]

Identifier: 37522278

GI number: 37522278

Start: 2869066

End: 2870001

Strand: Direct

Name: mjaIIIM [H]

Synonym: glr2709

Alternate gene names: 37522278

Gene position: 2869066-2870001 (Clockwise)

Preceding gene: 37522275

Following gene: 37522279

Centisome position: 61.58

GC content: 62.82

Gene sequence:

>936_bases
ATGACGGTTGCAAGCAGGGCAAGGCCCTTTCTCAAGTGGGCCGGGGGAAAAACCCAACTGCTCGATCAGATCGCCGAGCG
CTTCCCGGCGGTCCTCAAACACGGCCAAATCGATCGCTACGTCGAACCGTTCATCGGCGGCGGCGCTGTCTTTCTATACG
TCGCCCAGCGCTATGCGGTCGAACAATTCGTGCTCTTCGACATCAACCGCGAATTGATCCTGGCCTACCGGACCCTGCAG
AGGGCGGCGGACGATCTGATTGAAAAGCTCGAAGCGCTCGGGTTGCACTACCACGCCCTGGACGGCGACGAGCGGCGGGG
GTTTTTCTACCGGGTGCGCGAGCGGTTCAACACGCTCGCGGGTGAGATCGACTACGACCACTTCGACGGGCGCTGGGTGG
AGCGCACCGCCCAGATCATCTTCCTCAACCGCACCTGCTACAACGGCCTGTTTCGGATGAACACCAAGGCGCAGTTCAAC
GTTCCTTTCGGCCGCTACCGCAACCCCAGTATCTGCATGCCGGAGAACCTCAAGGCCGTCGCTGCCCTGCTCGCCCGTGC
GCGCATCGAATGGGGCGATTTTACCGACTGCGCGGCGCTGGCCGGGCCGGGGACGTTTATGTACTTCGATCCGCCCTACC
GGCCCCTGAGCAAGACGGCGCGCTTCACCGCCTACTCGGCTTTTGGTTTCGACGATGCCGAGCAGTTGCGCCTTGCACAG
CTGTATCGCACCCTCGATGCCGCTGGGGCAAAGCTGATGCTCAGCAATTGCGATCCGCTCAACACCGATCCGGCGGATGA
TTTTTTTGAGCGGGCCTACGCCGGCTTTGAGATCCGCCGGGTGCACGCCAGCCGTCTGGTCAACTGCCGGGCCGCTCGCC
GGGGCGTCATCACCGAGTTGCTCATCACCAACTACCCCCAGACTGCGGGCGGGTAG

Upstream 100 bases:

>100_bases
CGTCGGTACTATCGGGTATTGACGTCTCCAGCCACACAGGTTGCCATCCTTAGTTAAACATCTGCGGAGACTACACTTGA
TGCAAAGCGTCGGGGCGTCG

Downstream 100 bases:

>100_bases
GCCGCAGCGCCCGCTGGAGTAAGATCGCTCCATGCTGAAGGGTGTCAGTCTGTATCTGGTCGGCATGATGGGGTCCGGCA
AATCGACGGTGGGCCGGCTG

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTVASRARPFLKWAGGKTQLLDQIAERFPAVLKHGQIDRYVEPFIGGGAVFLYVAQRYAVEQFVLFDINRELILAYRTLQ
RAADDLIEKLEALGLHYHALDGDERRGFFYRVRERFNTLAGEIDYDHFDGRWVERTAQIIFLNRTCYNGLFRMNTKAQFN
VPFGRYRNPSICMPENLKAVAALLARARIEWGDFTDCAALAGPGTFMYFDPPYRPLSKTARFTAYSAFGFDDAEQLRLAQ
LYRTLDAAGAKLMLSNCDPLNTDPADDFFERAYAGFEIRRVHASRLVNCRAARRGVITELLITNYPQTAGG

Sequences:

>Translated_311_residues
MTVASRARPFLKWAGGKTQLLDQIAERFPAVLKHGQIDRYVEPFIGGGAVFLYVAQRYAVEQFVLFDINRELILAYRTLQ
RAADDLIEKLEALGLHYHALDGDERRGFFYRVRERFNTLAGEIDYDHFDGRWVERTAQIIFLNRTCYNGLFRMNTKAQFN
VPFGRYRNPSICMPENLKAVAALLARARIEWGDFTDCAALAGPGTFMYFDPPYRPLSKTARFTAYSAFGFDDAEQLRLAQ
LYRTLDAAGAKLMLSNCDPLNTDPADDFFERAYAGFEIRRVHASRLVNCRAARRGVITELLITNYPQTAGG
>Mature_310_residues
TVASRARPFLKWAGGKTQLLDQIAERFPAVLKHGQIDRYVEPFIGGGAVFLYVAQRYAVEQFVLFDINRELILAYRTLQR
AADDLIEKLEALGLHYHALDGDERRGFFYRVRERFNTLAGEIDYDHFDGRWVERTAQIIFLNRTCYNGLFRMNTKAQFNV
PFGRYRNPSICMPENLKAVAALLARARIEWGDFTDCAALAGPGTFMYFDPPYRPLSKTARFTAYSAFGFDDAEQLRLAQL
YRTLDAAGAKLMLSNCDPLNTDPADDFFERAYAGFEIRRVHASRLVNCRAARRGVITELLITNYPQTAGG

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=302, Percent_Identity=32.1192052980132, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35460; Mature: 35329

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVASRARPFLKWAGGKTQLLDQIAERFPAVLKHGQIDRYVEPFIGGGAVFLYVAQRYAV
CCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
EQFVLFDINRELILAYRTLQRAADDLIEKLEALGLHYHALDGDERRGFFYRVRERFNTLA
HHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
GEIDYDHFDGRWVERTAQIIFLNRTCYNGLFRMNTKAQFNVPFGRYRNPSICMPENLKAV
CCCCCCCCCCHHHHCEEEEEEEECHHHCCEEEECCCEEEECCCCCCCCCCCCCCCCHHHH
AALLARARIEWGDFTDCAALAGPGTFMYFDPPYRPLSKTARFTAYSAFGFDDAEQLRLAQ
HHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHEEEEECCCCCHHHHHHHH
LYRTLDAAGAKLMLSNCDPLNTDPADDFFERAYAGFEIRRVHASRLVNCRAARRGVITEL
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LITNYPQTAGG
HHHCCCCCCCC
>Mature Secondary Structure 
TVASRARPFLKWAGGKTQLLDQIAERFPAVLKHGQIDRYVEPFIGGGAVFLYVAQRYAV
CCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
EQFVLFDINRELILAYRTLQRAADDLIEKLEALGLHYHALDGDERRGFFYRVRERFNTLA
HHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
GEIDYDHFDGRWVERTAQIIFLNRTCYNGLFRMNTKAQFNVPFGRYRNPSICMPENLKAV
CCCCCCCCCCHHHHCEEEEEEEECHHHCCEEEECCCEEEECCCCCCCCCCCCCCCCHHHH
AALLARARIEWGDFTDCAALAGPGTFMYFDPPYRPLSKTARFTAYSAFGFDDAEQLRLAQ
HHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHEEEEECCCCCHHHHHHHH
LYRTLDAAGAKLMLSNCDPLNTDPADDFFERAYAGFEIRRVHASRLVNCRAARRGVITEL
HHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LITNYPQTAGG
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]