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The map label for this gene is aroK

Identifier: 37522279

GI number: 37522279

Start: 2870033

End: 2870566

Strand: Direct

Name: aroK

Synonym: gvip378

Alternate gene names: 37522279

Gene position: 2870033-2870566 (Clockwise)

Preceding gene: 37522278

Following gene: 37522280

Centisome position: 61.6

GC content: 67.6

Gene sequence:

>534_bases
ATGCTGAAGGGTGTCAGTCTGTATCTGGTCGGCATGATGGGGTCCGGCAAATCGACGGTGGGCCGGCTGCTCGCTGAAAA
GCTGGGCTACGGCTTTGTCGATCTCGACGCGCTCATCGAACAGGTAAGCGGCAAGCGGGTGGGCGAAATTTTTGAGCGCG
AGGGCGAAGCGGTTTTTCGTGATCTCGAAAGCCGGGTGCTCGCCGAGGTGAGCGCCTACACCCGTCTGGTCGTCGCCACC
GGCGGCGGCGTGGTGCTCGCCCGGCGCAACTGGGGCTATTTGCACCACGGAGTGGTCGTCTGGCTCGACGCCGATATCGA
GACGCTGCTCGGGCGGGTGGAGCACGAACCTGGCACCCGGCCCCTGCTTGCCGGCGGCGACAGGCACCTGCGCCTGGTCG
AACTGTTGGGCGAGCGCGCCAGGCTTTACGCCCAGGCGGACGTGCGCGTGAGCGCGGCGGGACTGCCGCCGGCAGTGGCC
GAGGAGACGCTGCGCTGTCTGGCGGCGCGTCTGGCGGAGGACAGTGCTCCGTGA

Upstream 100 bases:

>100_bases
CGGGCCGCTCGCCGGGGCGTCATCACCGAGTTGCTCATCACCAACTACCCCCAGACTGCGGGCGGGTAGGCCGCAGCGCC
CGCTGGAGTAAGATCGCTCC

Downstream 100 bases:

>100_bases
AACATGGACAGGCGTCCGCGCGCACCCTTAGGCTGAAGCAAAGTTTGGGAGGCGGCCATGGATGATCGACTGGAGGCGCA
TCTGCTGCGGCTTTGTGCCG

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFRDLESRVLAEVSAYTRLVVAT
GGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTRPLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVA
EETLRCLAARLAEDSAP

Sequences:

>Translated_177_residues
MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFRDLESRVLAEVSAYTRLVVAT
GGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTRPLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVA
EETLRCLAARLAEDSAP
>Mature_177_residues
MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFRDLESRVLAEVSAYTRLVVAT
GGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTRPLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVA
EETLRCLAARLAEDSAP

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=153, Percent_Identity=41.8300653594771, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786587, Length=169, Percent_Identity=36.6863905325444, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_GLOVI (Q7NH27)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925656.1
- ProteinModelPortal:   Q7NH27
- SMR:   Q7NH27
- GeneID:   2601111
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2710
- HOGENOM:   HBG335815
- OMA:   MIISERV
- ProtClustDB:   PRK00131
- BioCyc:   GVIO251221:GLR2710-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19103; Mature: 19103

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFR
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCHHHHHHHHCCHHHHH
DLESRVLAEVSAYTRLVVATGGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTR
HHHHHHHHHHHHHHEEEEEECCCEEEEECCCCEEECCEEEEECCCHHHHHHHHCCCCCCC
PLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVAEETLRCLAARLAEDSAP
CEEECCCCHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFR
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCHHHHHHHHCCHHHHH
DLESRVLAEVSAYTRLVVATGGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTR
HHHHHHHHHHHHHHEEEEEECCCEEEEECCCCEEECCEEEEECCCHHHHHHHHCCCCCCC
PLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVAEETLRCLAARLAEDSAP
CEEECCCCHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292