Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 37522235

GI number: 37522235

Start: 2827238

End: 2828395

Strand: Reverse

Name: pilT [H]

Synonym: gvip376

Alternate gene names: 37522235

Gene position: 2828395-2827238 (Counterclockwise)

Preceding gene: 37522236

Following gene: 37522234

Centisome position: 60.71

GC content: 65.98

Gene sequence:

>1158_bases
ATGCCCGTACCGACCATCCAGCAACTGATGCAGACCGTCGTCGACTGGTCGGGATCCGACCTGCACATCGCTGCCGGGAT
GCCGCCGTGCATCCGCGCCGGGGGCAAATTGCGCTTTGCGGGTGAATCGCCCCTCGCTCCCGACGACACCCAGGCGATGA
TCTTCAGCCTGCTCACCCCCGGCCAGCGCCAACACCTCGAAGCGCACTGGGAATTGGATTTCTCCCACGGTATCGGTGCC
ACCCGCTTTCGCATCAATGTCTACAAGGACAAGGGCCACTACGCCGCCGCCCTGCGTGCCCTCAGCTCGAAGATCCCGGC
CATCGACGAATTGGGCCTGCCGCCGGTGATGCGCGATATCGCCGACCGGCCCCGCGGATTGGTGCTGGTCACCGGTCCCA
CCGGCTCGGGCAAGACGACCACCCTCGCTTCGCTCATCGACTACATCAACACCAGCCGATCCGAACACATCCTCACCGTC
GAAGATCCGATCGAGTATCTGTTCGTGCCCAAAAAGTCCTTTATCAACCAGCGCCAGTTGGGCGAGGATACCAAGAGTTT
TGCCAACGCCCTGCGCGCCGCCCTGCGCGAGGATCCGGACGTCATCCTCGTCGGTGAGATGCGCGATCTGGAGACGATCC
AGCTGGCGATCACCGCCGCCGAGACGGGCCACCTGGTCTTTGCCACCCTGCACACCTCCTCCGCCGCCCAGACGGTCGAC
CGCATGGTCGATGTCTTCCCGCCCGCCCAGCAGCAGCAAATCCGCGTACAGCTTTCCAATTCGCTGGTGGCGGTCTTCGC
CCAAACGCTGATCCCGCGCGCCGACGCCGGCAGCGGCATCCAGTCGCGCTGCCTGGCGCAGGAGATCATGATCGTCACCC
CTGCCATCGCCAACCTCATCCGCGAGGGCAAGACCAGCCAGATCTACTCGGCCATCCAGACCGGCGGCAATCTGGGCATG
AAGACCCTGGAGACATCCCTGCGCGACCTGTACGCGGCGGGCCGGATTTCCTACGAGAACGCCCTCGCCCGCACCTCCAG
GCCCGAGGAGTTCCAGCGCATAGCCGGAGCCCCCCCGGCGGCGGGCCGTCCGGCCGTCGGCCCCGGCGGCGGCGGTGGTT
CCCGCCCCTCGTTCACCCAGCCCATCACCAACCGCTAA

Upstream 100 bases:

>100_bases
TGCCGCGGCTGCGGCGGCGAGTTGCACAGCGAATGGCCCGCCTGTCCCTACTGCACTTTGCCCCGGATCGATTAGCCAGC
GAGAGTTTCGGAGGAACGCC

Downstream 100 bases:

>100_bases
GCTGCCTTTGAGCGACCGCTATGCCGCAATTCAAGTACCGCGTGCGCGACTACCGGGGGACCGCCATCGAGGAGACCGTC
GAGGCCGACAGCGCCGTCAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MPVPTIQQLMQTVVDWSGSDLHIAAGMPPCIRAGGKLRFAGESPLAPDDTQAMIFSLLTPGQRQHLEAHWELDFSHGIGA
TRFRINVYKDKGHYAAALRALSSKIPAIDELGLPPVMRDIADRPRGLVLVTGPTGSGKTTTLASLIDYINTSRSEHILTV
EDPIEYLFVPKKSFINQRQLGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPPAQQQQIRVQLSNSLVAVFAQTLIPRADAGSGIQSRCLAQEIMIVTPAIANLIREGKTSQIYSAIQTGGNLGM
KTLETSLRDLYAAGRISYENALARTSRPEEFQRIAGAPPAAGRPAVGPGGGGGSRPSFTQPITNR

Sequences:

>Translated_385_residues
MPVPTIQQLMQTVVDWSGSDLHIAAGMPPCIRAGGKLRFAGESPLAPDDTQAMIFSLLTPGQRQHLEAHWELDFSHGIGA
TRFRINVYKDKGHYAAALRALSSKIPAIDELGLPPVMRDIADRPRGLVLVTGPTGSGKTTTLASLIDYINTSRSEHILTV
EDPIEYLFVPKKSFINQRQLGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPPAQQQQIRVQLSNSLVAVFAQTLIPRADAGSGIQSRCLAQEIMIVTPAIANLIREGKTSQIYSAIQTGGNLGM
KTLETSLRDLYAAGRISYENALARTSRPEEFQRIAGAPPAAGRPAVGPGGGGGSRPSFTQPITNR
>Mature_384_residues
PVPTIQQLMQTVVDWSGSDLHIAAGMPPCIRAGGKLRFAGESPLAPDDTQAMIFSLLTPGQRQHLEAHWELDFSHGIGAT
RFRINVYKDKGHYAAALRALSSKIPAIDELGLPPVMRDIADRPRGLVLVTGPTGSGKTTTLASLIDYINTSRSEHILTVE
DPIEYLFVPKKSFINQRQLGEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVDR
MVDVFPPAQQQQIRVQLSNSLVAVFAQTLIPRADAGSGIQSRCLAQEIMIVTPAIANLIREGKTSQIYSAIQTGGNLGMK
TLETSLRDLYAAGRISYENALARTSRPEEFQRIAGAPPAAGRPAVGPGGGGGSRPSFTQPITNR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=333, Percent_Identity=43.2432432432432, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1786296, Length=325, Percent_Identity=33.8461538461538, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=46.2025316455696, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41479; Mature: 41348

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPTIQQLMQTVVDWSGSDLHIAAGMPPCIRAGGKLRFAGESPLAPDDTQAMIFSLLTP
CCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCC
GQRQHLEAHWELDFSHGIGATRFRINVYKDKGHYAAALRALSSKIPAIDELGLPPVMRDI
CHHHCCEEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHH
ADRPRGLVLVTGPTGSGKTTTLASLIDYINTSRSEHILTVEDPIEYLFVPKKSFINQRQL
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHHC
GEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCHHHHHHH
RMVDVFPPAQQQQIRVQLSNSLVAVFAQTLIPRADAGSGIQSRCLAQEIMIVTPAIANLI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
REGKTSQIYSAIQTGGNLGMKTLETSLRDLYAAGRISYENALARTSRPEEFQRIAGAPPA
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCCC
AGRPAVGPGGGGGSRPSFTQPITNR
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVPTIQQLMQTVVDWSGSDLHIAAGMPPCIRAGGKLRFAGESPLAPDDTQAMIFSLLTP
CCHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCC
GQRQHLEAHWELDFSHGIGATRFRINVYKDKGHYAAALRALSSKIPAIDELGLPPVMRDI
CHHHCCEEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHHHHH
ADRPRGLVLVTGPTGSGKTTTLASLIDYINTSRSEHILTVEDPIEYLFVPKKSFINQRQL
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHHC
GEDTKSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEEEECCHHHHHHH
RMVDVFPPAQQQQIRVQLSNSLVAVFAQTLIPRADAGSGIQSRCLAQEIMIVTPAIANLI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
REGKTSQIYSAIQTGGNLGMKTLETSLRDLYAAGRISYENALARTSRPEEFQRIAGAPPA
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCCC
AGRPAVGPGGGGGSRPSFTQPITNR
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]