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The map label for this gene is pilB [H]

Identifier: 37522236

GI number: 37522236

Start: 2828421

End: 2830349

Strand: Reverse

Name: pilB [H]

Synonym: gvip377

Alternate gene names: 37522236

Gene position: 2830349-2828421 (Counterclockwise)

Preceding gene: 37522239

Following gene: 37522235

Centisome position: 60.75

GC content: 68.48

Gene sequence:

>1929_bases
GTGGGCGATCTTGCGCACCAGGCCCAGGTTGCGGCAGACCAGTTCGTTGCGCAGTGCGGTGGAAGGAGATTCGCCGTAGG
CGCGCAACAACAGCCGGGTCGGCCCGGCAAATTTCGGGGTGCCTGTCATAAAAGCCTTGGGTTATCAAGGTGGTCGTGGA
CCGCATCCTACCGGGGTGCCCGGCCCGGCCGGGTCGATTGCGCCTCCAGGGCGGCCCGGTTTGTTCGGCGCCGCACGGGT
GAGAAACCGGCACCGTCGCGGGGTACGCTCTGTTTGGCGTTTACCCGGATTGGGTTCATGGCATCCGCCCAGCGATTGTT
CGAATTGAAGATCCGCTACGGCGTCGCCGCCTTCGACCCCGGCGGGGTCGATCCGGTTCGCCTGCGGCGGTTGGTCGAGA
GCTGGCTATCTATCGAAAGTTGCGCCCACTACCGGCTGCTGCCCGTGCGCGAGGAGGAGGGGGTGCTGGCCGTGGCGATG
GTCGATCCTGAGCGCCTCGAAGCCCTCGACGAACTGCAGCGGCGGGGGCGGTTGCGCGGTCTCGCCCTGCGCCGTCTTGC
CATCGACGCCCAGGATTTTGCCACGTTGCTTGCAGCCTGCCAGGCAGCGCCGATGGGGACGGATCCGGAGTCGGCGGCGG
GCGAGCTGGAGGCGCCCGTGGTGCGGCTTTCCTCCCAGATTCTCGATCGGGCGGTGGCGGAAGGGGCCTCCGACATCCAC
ATCGAACCGCAGCCCGCCGGGGTGCGCGTCCGCCTGCGCAGGGACGGTGTCCTGCACGAGGCCGGCGAACCGCTGAGCGC
CGAGCTGGGATTGGCGCTGGTGTCGCGCTTCAAGATCCTCTCGGAACTGGATATTGCCGAGCGCCGTCTGCCCCAAGACG
GCCGTTTGCACCGCCGCGTCGGGGAGCGGGCCGTCGATTTTCGCGTCTCGACGTTGCCGGGGCGCTTCGGAGAGAAAGTG
GTGCTGAGGCTGCTCGACAACGCCGCCACCCGCTTGGGGCTCGACCAGTTGATTGGCGAGGCCGGGACGCTCACGGCGGT
GCGCGAATTGATTCGTCGCCCCCACGGGCTGTTTCTGGTCACCGGTCCCACCGGCTGCGGCAAGACGACCACCCTCTACG
CGGCGCTTGCCGAGCGCAACGAGCCGGGGGTCAACATCGCCACCGCCGAAGATCCGGTGGAGTACACCCTGGCGGGGATC
ACCCAGGTGCAGGTAATCCGCGAGAAGGGCCTCGATTTTGCCCGCATCCTGCGCGCGCTGCTCCGCCAAGATCCCGACGT
CGTGCTGGTGGGGGAGACCCGCGACCGCGAGACCGCCCAGACCGCCATCGAAGCGGCCCTCACCGGTCACCTGGTGCTCA
CCAGCCTGCACACCAACGACGCGGCCGGAGCGGTGGCGCGCCTCAGGGAGCTGGGGGTGGCCCCCTATTTGATTGCCAGT
GCCCTGGTGGGCGTGCTCGCCCAGCGGCTGTTGCGCCGGCTTTGCGGCGAATGCAAAGAGGCGTATGTACCGCCTCCTGA
GCGCCTCGCCCGCTTTGGCCTCACAGGCGGGACGTACTTTCGAGTGCGGGGGCAGAGGGGAGCAGCCTGCCCCGGCTGCG
GCGGCGTAGGTTATAAAGGGCGCGTCGGAGTTTACGAAATCATGTCCCTGGGAGAACACCTGCGCCAACTGATCGGCCGC
GAAGCCACCACCCAGACGCTGCGCGAGGCGGCGGCGGGGGGCGGGATGCGTTCGCTGCTGGCAGGTTCTCTAGATCTGGC
GCGCGCGGGGGTGACCAGCCTGGAGGAAGTCGAGCGCGTCACCCTGTCCGACACCGACCAAGCGGGAAGCCCCGCGGCCG
CGAGCCACCCCATCTGCCGCGGCTGCGGCGGCGAGTTGCACAGCGAATGGCCCGCCTGTCCCTACTGCACTTTGCCCCGG
ATCGATTAG

Upstream 100 bases:

>100_bases
GGCCCAAAGACGGGTCGAAGCGCTCGACGGCGCGCATCAGGCCGATGGCGCCCACCTGCACGAGGTCTTCCAACGGCTCG
CTGCTGCCGCGGGCCAGTTG

Downstream 100 bases:

>100_bases
CCAGCGAGAGTTTCGGAGGAACGCCATGCCCGTACCGACCATCCAGCAACTGATGCAGACCGTCGTCGACTGGTCGGGAT
CCGACCTGCACATCGCTGCC

Product: pilus biogenesis protein homologous

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MGDLAHQAQVAADQFVAQCGGRRFAVGAQQQPGRPGKFRGACHKSLGLSRWSWTASYRGARPGRVDCASRAARFVRRRTG
EKPAPSRGTLCLAFTRIGFMASAQRLFELKIRYGVAAFDPGGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAM
VDPERLEALDELQRRGRLRGLALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIH
IEPQPAGVRVRLRRDGVLHEAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRVGERAVDFRVSTLPGRFGEKV
VLRLLDNAATRLGLDQLIGEAGTLTAVRELIRRPHGLFLVTGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGI
TQVQVIREKGLDFARILRALLRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIAS
ALVGVLAQRLLRRLCGECKEAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKGRVGVYEIMSLGEHLRQLIGR
EATTQTLREAAAGGGMRSLLAGSLDLARAGVTSLEEVERVTLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPR
ID

Sequences:

>Translated_642_residues
MGDLAHQAQVAADQFVAQCGGRRFAVGAQQQPGRPGKFRGACHKSLGLSRWSWTASYRGARPGRVDCASRAARFVRRRTG
EKPAPSRGTLCLAFTRIGFMASAQRLFELKIRYGVAAFDPGGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAM
VDPERLEALDELQRRGRLRGLALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIH
IEPQPAGVRVRLRRDGVLHEAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRVGERAVDFRVSTLPGRFGEKV
VLRLLDNAATRLGLDQLIGEAGTLTAVRELIRRPHGLFLVTGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGI
TQVQVIREKGLDFARILRALLRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIAS
ALVGVLAQRLLRRLCGECKEAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKGRVGVYEIMSLGEHLRQLIGR
EATTQTLREAAAGGGMRSLLAGSLDLARAGVTSLEEVERVTLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPR
ID
>Mature_641_residues
GDLAHQAQVAADQFVAQCGGRRFAVGAQQQPGRPGKFRGACHKSLGLSRWSWTASYRGARPGRVDCASRAARFVRRRTGE
KPAPSRGTLCLAFTRIGFMASAQRLFELKIRYGVAAFDPGGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAMV
DPERLEALDELQRRGRLRGLALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIHI
EPQPAGVRVRLRRDGVLHEAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRVGERAVDFRVSTLPGRFGEKVV
LRLLDNAATRLGLDQLIGEAGTLTAVRELIRRPHGLFLVTGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGIT
QVQVIREKGLDFARILRALLRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIASA
LVGVLAQRLLRRLCGECKEAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKGRVGVYEIMSLGEHLRQLIGRE
ATTQTLREAAAGGGMRSLLAGSLDLARAGVTSLEEVERVTLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPRI
D

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=419, Percent_Identity=45.8233890214797, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI1786296, Length=462, Percent_Identity=39.8268398268398, Blast_Score=298, Evalue=1e-81,
Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=44.3298969072165, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 69203; Mature: 69072

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLAHQAQVAADQFVAQCGGRRFAVGAQQQPGRPGKFRGACHKSLGLSRWSWTASYRGA
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCEECCCCCCC
RPGRVDCASRAARFVRRRTGEKPAPSRGTLCLAFTRIGFMASAQRLFELKIRYGVAAFDP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCEEECCC
GGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAMVDPERLEALDELQRRGRLRG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCHHHH
LALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIH
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
IEPQPAGVRVRLRRDGVLHEAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRV
ECCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GERAVDFRVSTLPGRFGEKVVLRLLDNAATRLGLDQLIGEAGTLTAVRELIRRPHGLFLV
CCHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEEE
TGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGITQVQVIREKGLDFARILRAL
ECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIAS
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCHHHHHHH
ALVGVLAQRLLRRLCGECKEAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
RVGVYEIMSLGEHLRQLIGREATTQTLREAAAGGGMRSLLAGSLDLARAGVTSLEEVERV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHCCHHHHHHHHH
TLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPRID
HCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GDLAHQAQVAADQFVAQCGGRRFAVGAQQQPGRPGKFRGACHKSLGLSRWSWTASYRGA
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCEECCCCCCC
RPGRVDCASRAARFVRRRTGEKPAPSRGTLCLAFTRIGFMASAQRLFELKIRYGVAAFDP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCEEECCC
GGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAMVDPERLEALDELQRRGRLRG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCHHHH
LALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIH
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
IEPQPAGVRVRLRRDGVLHEAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRV
ECCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GERAVDFRVSTLPGRFGEKVVLRLLDNAATRLGLDQLIGEAGTLTAVRELIRRPHGLFLV
CCHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCEEEE
TGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGITQVQVIREKGLDFARILRAL
ECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIAS
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCHHHHHHH
ALVGVLAQRLLRRLCGECKEAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
RVGVYEIMSLGEHLRQLIGREATTQTLREAAAGGGMRSLLAGSLDLARAGVTSLEEVERV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHCCHHHHHHHHH
TLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPRID
HCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]