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The map label for this gene is murF [H]

Identifier: 37521967

GI number: 37521967

Start: 2558608

End: 2559960

Strand: Direct

Name: murF [H]

Synonym: gvip335

Alternate gene names: 37521967

Gene position: 2558608-2559960 (Clockwise)

Preceding gene: 37521966

Following gene: 37521968

Centisome position: 54.92

GC content: 67.7

Gene sequence:

>1353_bases
ATGTTTGAAATCGACACACAGACACTGCAAACCCTCCTGGGCGCCGAGGCGGAAGGAACGCTTCCGATGTCGGTGCGGGG
CGTCGGGACCGACACACGCGCCCTCGCGCCGGGAGCGCTGTTCGTGGCGCTTCGGGGCGAACGCTTCGACGGCCACGACT
ACGCGCGGCAGGCGGTGGCCGGGGGGGCGGCCCTGGTGATTGCCGAGCGGCCGGTGGCGGTGCCTCATCTGCGGGTGGCC
GACACCCTGGAGGCTTATCAGACGCTGGCGCGCTGGTGGCGGGATCGCTGCACGCTGCCGGTGGTGGCCGTCACCGGTTC
GGCCGGCAAAACCACCACCCGCGAACTGATTCGCGCCGTGCTCGCCACCGCCGGACCGGTCCTCGCCCCGCCCGGCAACG
AAAACAACGACGTGGGCGTGCCAAAGTTGCTGCTCCAACTCACCGGTGCCCACCGCTACTGCGTGGTCGAGATGGGCATG
CGCGGCCCGGGGGAGATTGCTCGCCTCGCCCGCTGTGCCGCCCCGAATGTCGGGGTGATTACCAACGTCGGCAGCGCCCA
TATCGGACGGCTTGGCTCCCACGCCGCTATCGCCGACGCCAAGTGCGAACTGCTCGCCGAGCTGCCGTGCTCAGGGATCG
CCGTTCTCAACGGCGAAGACGAGCGGCTGCTTTCCACCGCCCGCCGCCACTGGGCGGGGCCGGTGCTCACCTATGGTCTT
GATGCCGGGGATGTTATGGGCAGCTTCGACGGCGTGCACCTGCACGTCGATGGGATCGCCTTTATTCCACCCCTGCCGGG
CCGCCATAACCACCTCAACTTCCTGGCCGCCCTGGCGGTCGCCCGCCACCTCGGGTTGGAGTTGCTCCAGGTGCGCGAGC
GCCTCAAAGATCTTCAGTTACCCACCGGCCGCAGCCGGGTGATCGCCCTTGAAGGCGACGTGCAACTGATTGACGAAACC
TACAACAGCGCCCCCGAATCGGCCCGCGCCGCCCTTGCATGGCTTGCCGGCCTGCCCGGCCGCCGGATCGCGGTTCTAGG
ACAGATGCGTGAATTGGGCGAATTCAGCCGGGAGCTGCATCGCGTCCTGGGCGAGCACTGCCGCCGTCTGGCCCTCGACG
AAGTGGTGGTTCTGGAGGCGGGCGAGGACACCGAAGCGTTGCTTGCAGGAGCCGGAGATCTTCCGGCGGTACAGCTTGCC
GACCACCGGGCGGTGGCCGCATATCTGGCAGATCGGGTGCGACCGGGGGATCGCCTTTTATTTAAGGCTTCGCGGGCGGT
GGGTCTGGAGCGGGTGCTGGCGGAGTTTCAGGATTTGTTCCGGGCAGGTACGCCGTCTGAGCGTGCACAATAG

Upstream 100 bases:

>100_bases
GCATCTACACGTCGGCCAGGAACTGGTGGGGATCGTCACCGACCTGCGCCGCCAGGCGACCGAAGCGGCTGAGCGCCTGC
TGCAGATGGGATAGTGCCCT

Downstream 100 bases:

>100_bases
AGCCAATCGATTTCGATCATTCCGTATGACCACCAGCAGCAGCACTGCGCCTACGCCCTACCGGCTGACCGGCATCAGCC
CCAAAGCCTACGAGCACCCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MFEIDTQTLQTLLGAEAEGTLPMSVRGVGTDTRALAPGALFVALRGERFDGHDYARQAVAGGAALVIAERPVAVPHLRVA
DTLEAYQTLARWWRDRCTLPVVAVTGSAGKTTTRELIRAVLATAGPVLAPPGNENNDVGVPKLLLQLTGAHRYCVVEMGM
RGPGEIARLARCAAPNVGVITNVGSAHIGRLGSHAAIADAKCELLAELPCSGIAVLNGEDERLLSTARRHWAGPVLTYGL
DAGDVMGSFDGVHLHVDGIAFIPPLPGRHNHLNFLAALAVARHLGLELLQVRERLKDLQLPTGRSRVIALEGDVQLIDET
YNSAPESARAALAWLAGLPGRRIAVLGQMRELGEFSRELHRVLGEHCRRLALDEVVVLEAGEDTEALLAGAGDLPAVQLA
DHRAVAAYLADRVRPGDRLLFKASRAVGLERVLAEFQDLFRAGTPSERAQ

Sequences:

>Translated_450_residues
MFEIDTQTLQTLLGAEAEGTLPMSVRGVGTDTRALAPGALFVALRGERFDGHDYARQAVAGGAALVIAERPVAVPHLRVA
DTLEAYQTLARWWRDRCTLPVVAVTGSAGKTTTRELIRAVLATAGPVLAPPGNENNDVGVPKLLLQLTGAHRYCVVEMGM
RGPGEIARLARCAAPNVGVITNVGSAHIGRLGSHAAIADAKCELLAELPCSGIAVLNGEDERLLSTARRHWAGPVLTYGL
DAGDVMGSFDGVHLHVDGIAFIPPLPGRHNHLNFLAALAVARHLGLELLQVRERLKDLQLPTGRSRVIALEGDVQLIDET
YNSAPESARAALAWLAGLPGRRIAVLGQMRELGEFSRELHRVLGEHCRRLALDEVVVLEAGEDTEALLAGAGDLPAVQLA
DHRAVAAYLADRVRPGDRLLFKASRAVGLERVLAEFQDLFRAGTPSERAQ
>Mature_450_residues
MFEIDTQTLQTLLGAEAEGTLPMSVRGVGTDTRALAPGALFVALRGERFDGHDYARQAVAGGAALVIAERPVAVPHLRVA
DTLEAYQTLARWWRDRCTLPVVAVTGSAGKTTTRELIRAVLATAGPVLAPPGNENNDVGVPKLLLQLTGAHRYCVVEMGM
RGPGEIARLARCAAPNVGVITNVGSAHIGRLGSHAAIADAKCELLAELPCSGIAVLNGEDERLLSTARRHWAGPVLTYGL
DAGDVMGSFDGVHLHVDGIAFIPPLPGRHNHLNFLAALAVARHLGLELLQVRERLKDLQLPTGRSRVIALEGDVQLIDET
YNSAPESARAALAWLAGLPGRRIAVLGQMRELGEFSRELHRVLGEHCRRLALDEVVVLEAGEDTEALLAGAGDLPAVQLA
DHRAVAAYLADRVRPGDRLLFKASRAVGLERVLAEFQDLFRAGTPSERAQ

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=457, Percent_Identity=35.0109409190372, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48044; Mature: 48044

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIDTQTLQTLLGAEAEGTLPMSVRGVGTDTRALAPGALFVALRGERFDGHDYARQAVA
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHC
GGAALVIAERPVAVPHLRVADTLEAYQTLARWWRDRCTLPVVAVTGSAGKTTTRELIRAV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LATAGPVLAPPGNENNDVGVPKLLLQLTGAHRYCVVEMGMRGPGEIARLARCAAPNVGVI
HHHCCCEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEE
TNVGSAHIGRLGSHAAIADAKCELLAELPCSGIAVLNGEDERLLSTARRHWAGPVLTYGL
ECCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEECC
DAGDVMGSFDGVHLHVDGIAFIPPLPGRHNHLNFLAALAVARHLGLELLQVRERLKDLQL
CHHHHHCCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTGRSRVIALEGDVQLIDETYNSAPESARAALAWLAGLPGRRIAVLGQMRELGEFSRELH
CCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RVLGEHCRRLALDEVVVLEAGEDTEALLAGAGDLPAVQLADHRAVAAYLADRVRPGDRLL
HHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCEEEHHCHHHHHHHHHHHCCCCCHHH
FKASRAVGLERVLAEFQDLFRAGTPSERAQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MFEIDTQTLQTLLGAEAEGTLPMSVRGVGTDTRALAPGALFVALRGERFDGHDYARQAVA
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHC
GGAALVIAERPVAVPHLRVADTLEAYQTLARWWRDRCTLPVVAVTGSAGKTTTRELIRAV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LATAGPVLAPPGNENNDVGVPKLLLQLTGAHRYCVVEMGMRGPGEIARLARCAAPNVGVI
HHHCCCEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEE
TNVGSAHIGRLGSHAAIADAKCELLAELPCSGIAVLNGEDERLLSTARRHWAGPVLTYGL
ECCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEECC
DAGDVMGSFDGVHLHVDGIAFIPPLPGRHNHLNFLAALAVARHLGLELLQVRERLKDLQL
CHHHHHCCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTGRSRVIALEGDVQLIDETYNSAPESARAALAWLAGLPGRRIAVLGQMRELGEFSRELH
CCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RVLGEHCRRLALDEVVVLEAGEDTEALLAGAGDLPAVQLADHRAVAAYLADRVRPGDRLL
HHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCEEEHHCHHHHHHHHHHHCCCCCHHH
FKASRAVGLERVLAEFQDLFRAGTPSERAQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]