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The map label for this gene is hisZ

Identifier: 37521959

GI number: 37521959

Start: 2550809

End: 2551987

Strand: Direct

Name: hisZ

Synonym: gvip334

Alternate gene names: 37521959

Gene position: 2550809-2551987 (Clockwise)

Preceding gene: 37521958

Following gene: 37521960

Centisome position: 54.75

GC content: 67.68

Gene sequence:

>1179_bases
ATGTACCAACCGCCGACCGGGGTGCGCGACTTGCTGCCCCTCGACGTCTCGCAAAAGCTCTGGATCGAACAGCGCCTGCA
GCGGGTGTTCACCCGCTGGGGCTACCAGCGCATCATCACTCCGACCCTCGAACGGATGGAGACGCTGCAGGCGAGCGGTT
CGGTCGACCTGCAGGCGATCTTGCAGCTGCGCGACGCCGAGGGGGTGTCGCTGGGCCTCAGACCCGACCCGACGCCGTCG
CTGGCGCGGGCGGTGGCCACGCGCCTTGCCGATGCGCCCTTGCCGGTGCGGCTCTCGTATCAGATGAACGTCTTTCGCAG
CACCACGCAGCCGCAGGAATTTTACCAGGCCGGGGTGGAACTGATCGGCGCGGGCGGGGTGCTCGCCGACGCCGAGGTGC
TGCTTGTACTCGCCGAGTGCCTGGCCGAACTGGCTCCGCCAGATTGGACGCTGATTTTGGGCGCGGTCGCGTTTACCCGT
TCGTGGCTTGCCCAGGTGGCAGAACCCGCCCGCCACCGGCTGCGCCGGGCGATGGCCGAACTCGACCGCGTGGCGATTCT
TGCTGAAGCGGGCATCGACGAGGCGGTCAGATCCCAGTTGTTGTTGTTGTTTGACCTGCGCGGCGAACCGGAAATGGTGC
TCAGCAAGGCGTCCTCGCTGCCGATGAGCGACGCCCAGCGCGCGGAACTCGCCGAACTGGAGACGCTCACCGGCTGGCTG
AGGGGCCGCTCGGTGCCGGTGGTGCTCGATTTGAGCCTGGTCGAAGCGTTCGATTACTACACGGGCCTCATCTTCGAGGT
GAGCGCCGGGGGGCGTCTGATCGGCCGTGGGGGCCGCTATGACCATCTGCTGGGTTCCTACGGCAAACCGGCGCCGGGGG
CGGGCTTTGCCCTGAACCTCGAAGCGCTCCAGCAGGTACTCTTGCCCACCGGCAAACTGCCCGGCCGCACGGTGGGGGGT
GGCTTTTTGGTGGTGCCCGACGGGCCGGACGCCTGGGAGGCGGCCCTGGCTGAGGCGGATAAGCTGCGCTGCGCTCCCGG
CGAGCGGGTGGAGATTGAACTGTTGGGCCGCACCGGTGAGGAGGCAATTGCCCACGGTCGGGCTCTGGGTGCGGCCGTCG
TGCGCTGGGTTCACCCCGACGGTAGTACCACCGATTGCGACCTTGCGGTCATACAGTGA

Upstream 100 bases:

>100_bases
CCAATCCGACAAGCTGCTCATCAAAGCAAAGGCGCTTTCCGGCAGGCTAATCTCCCCCACCCCAGCCGTACAGTAGGAAA
GGACACAACTCGGTTCTCCC

Downstream 100 bases:

>100_bases
TACCTGCCACCCACAAAACTGAAAAAATACGAACCGACAATCGGTCTAACGCGCTTTTGGTGATGCAGGAAAATAAGTAC
CATGACTGACCGTCGCTATG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MYQPPTGVRDLLPLDVSQKLWIEQRLQRVFTRWGYQRIITPTLERMETLQASGSVDLQAILQLRDAEGVSLGLRPDPTPS
LARAVATRLADAPLPVRLSYQMNVFRSTTQPQEFYQAGVELIGAGGVLADAEVLLVLAECLAELAPPDWTLILGAVAFTR
SWLAQVAEPARHRLRRAMAELDRVAILAEAGIDEAVRSQLLLLFDLRGEPEMVLSKASSLPMSDAQRAELAELETLTGWL
RGRSVPVVLDLSLVEAFDYYTGLIFEVSAGGRLIGRGGRYDHLLGSYGKPAPGAGFALNLEALQQVLLPTGKLPGRTVGG
GFLVVPDGPDAWEAALAEADKLRCAPGERVEIELLGRTGEEAIAHGRALGAAVVRWVHPDGSTTDCDLAVIQ

Sequences:

>Translated_392_residues
MYQPPTGVRDLLPLDVSQKLWIEQRLQRVFTRWGYQRIITPTLERMETLQASGSVDLQAILQLRDAEGVSLGLRPDPTPS
LARAVATRLADAPLPVRLSYQMNVFRSTTQPQEFYQAGVELIGAGGVLADAEVLLVLAECLAELAPPDWTLILGAVAFTR
SWLAQVAEPARHRLRRAMAELDRVAILAEAGIDEAVRSQLLLLFDLRGEPEMVLSKASSLPMSDAQRAELAELETLTGWL
RGRSVPVVLDLSLVEAFDYYTGLIFEVSAGGRLIGRGGRYDHLLGSYGKPAPGAGFALNLEALQQVLLPTGKLPGRTVGG
GFLVVPDGPDAWEAALAEADKLRCAPGERVEIELLGRTGEEAIAHGRALGAAVVRWVHPDGSTTDCDLAVIQ
>Mature_392_residues
MYQPPTGVRDLLPLDVSQKLWIEQRLQRVFTRWGYQRIITPTLERMETLQASGSVDLQAILQLRDAEGVSLGLRPDPTPS
LARAVATRLADAPLPVRLSYQMNVFRSTTQPQEFYQAGVELIGAGGVLADAEVLLVLAECLAELAPPDWTLILGAVAFTR
SWLAQVAEPARHRLRRAMAELDRVAILAEAGIDEAVRSQLLLLFDLRGEPEMVLSKASSLPMSDAQRAELAELETLTGWL
RGRSVPVVLDLSLVEAFDYYTGLIFEVSAGGRLIGRGGRYDHLLGSYGKPAPGAGFALNLEALQQVLLPTGKLPGRTVGG
GFLVVPDGPDAWEAALAEADKLRCAPGERVEIELLGRTGEEAIAHGRALGAAVVRWVHPDGSTTDCDLAVIQ

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=336, Percent_Identity=25.5952380952381, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325290, Length=356, Percent_Identity=24.438202247191, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24643061, Length=354, Percent_Identity=25.7062146892655, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24643059, Length=373, Percent_Identity=24.6648793565684, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_GLOVI (Q7NHZ4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925336.1
- ProteinModelPortal:   Q7NHZ4
- SMR:   Q7NHZ4
- GeneID:   2600791
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2390
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- BioCyc:   GVIO251221:GLR2390-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 42421; Mature: 42421

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQPPTGVRDLLPLDVSQKLWIEQRLQRVFTRWGYQRIITPTLERMETLQASGSVDLQAI
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
LQLRDAEGVSLGLRPDPTPSLARAVATRLADAPLPVRLSYQMNVFRSTTQPQEFYQAGVE
HHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCHHHHHHHHHH
LIGAGGVLADAEVLLVLAECLAELAPPDWTLILGAVAFTRSWLAQVAEPARHRLRRAMAE
EEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRVAILAEAGIDEAVRSQLLLLFDLRGEPEMVLSKASSLPMSDAQRAELAELETLTGWL
HHHHHHHHHCCCCHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RGRSVPVVLDLSLVEAFDYYTGLIFEVSAGGRLIGRGGRYDHLLGSYGKPAPGAGFALNL
CCCCCCEEEEHHHHHHHHHHHCEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCCEEEEH
EALQQVLLPTGKLPGRTVGGGFLVVPDGPDAWEAALAEADKLRCAPGERVEIELLGRTGE
HHHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCH
EAIAHGRALGAAVVRWVHPDGSTTDCDLAVIQ
HHHHHHHHHHHHHHEEECCCCCCCCCCEEEEC
>Mature Secondary Structure
MYQPPTGVRDLLPLDVSQKLWIEQRLQRVFTRWGYQRIITPTLERMETLQASGSVDLQAI
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
LQLRDAEGVSLGLRPDPTPSLARAVATRLADAPLPVRLSYQMNVFRSTTQPQEFYQAGVE
HHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCHHHHHHHHHH
LIGAGGVLADAEVLLVLAECLAELAPPDWTLILGAVAFTRSWLAQVAEPARHRLRRAMAE
EEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRVAILAEAGIDEAVRSQLLLLFDLRGEPEMVLSKASSLPMSDAQRAELAELETLTGWL
HHHHHHHHHCCCCHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RGRSVPVVLDLSLVEAFDYYTGLIFEVSAGGRLIGRGGRYDHLLGSYGKPAPGAGFALNL
CCCCCCEEEEHHHHHHHHHHHCEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCCEEEEH
EALQQVLLPTGKLPGRTVGGGFLVVPDGPDAWEAALAEADKLRCAPGERVEIELLGRTGE
HHHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCH
EAIAHGRALGAAVVRWVHPDGSTTDCDLAVIQ
HHHHHHHHHHHHHHEEECCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292