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The map label for this gene is gltX

Identifier: 37521947

GI number: 37521947

Start: 2536374

End: 2537966

Strand: Reverse

Name: gltX

Synonym: gvip330

Alternate gene names: 37521947

Gene position: 2537966-2536374 (Counterclockwise)

Preceding gene: 37521952

Following gene: 37521943

Centisome position: 54.47

GC content: 63.78

Gene sequence:

>1593_bases
ATGAATTTTGAGCAGTACGAGTCCGCCTACCCGCCCCGGGCTTTACCCCCGGGCGCGTGGGTGACCCGCATCGGGCCGAG
CCCGACGGGCAAGGCCCACATCGGCACGGCCCTGCAGGCGGTGATCAACCGGTCCCTGGCCGACGGCAGCGGCGGCGCCT
TTTTGCTGCGCATCGAGGACACCGACCGCGAGCGCTTCGTCGAAGGCGCCATCGAAGATCTGCTCGCGGCCCTCGACTGG
TTGGGGATCCGGCCGGACGAAAGCGTCGCCCATGGCGGGGCCTACGGCCCCTATGTCCAGAGCGAGCGGCTGCCCATCTA
CCAGGCGGCCGCCCGGCAACTGGTGGAAGTGGGGCACGCCTACTATTGTTTTTGCACGCCGGAGCGGCTCGAATCGGTGC
GCATGGCCCAGCAGGCGGCGGGCAAACCGACCATGTACGATCGCCACTGCCGCAATCTGGGTCGCGAGGAAGTCGAGAAG
CGGCTCGCGACCGGTGAAAAAGCGGTCGTGCGCCTGAAGGTGCCCGACGGCACCGAGATCGCCTTCGACGATCTGGCGCG
CGGCCGGATCGCCTTCGAGTCCCAGACGATCGACGACAGCGTGCTGCTCAAGTCCGACGGCTTTCCCACCTACCACCTGG
CGGTGGTGGTGGACGATCACCTGATGCGGGTGAATCAGATTATCCGTGGCGAGGAGTGGATTCCTTCGACCCCCAAGCAC
GTGCTTTTGTACCAGTACTTTGGCTGGCCGATGCCGCGCATCGCCCACACGCCCATCCTGCGCGACATGAGCCGCCGCAA
ACTCTCCAAGCGCAGCGGCGACACGTCGATCACCGGCTATCGGGTGCAGGGGTACTTGCCCGAGGGGCTGCGCAACTTTC
TCACGCGGATCATCTGGCCCCACCCGGAGGGCAAGGACGTCTACGACCACGAAGAATTCGTGCGGTTATTCAAGGCGGAA
GATCTGCCCAACACCGGTCCCATCGTCGATCCACAGCTGCTCGATTTCATCAACGGCCAGTATCTGCGCCGACTTTCGTC
TGCAGAACTTTACACCGCGACGGTGGAATATCTGAAATTCTTGCTGGGCTTGCATCAGGACATCATCTTCGAGACTTACC
ACCCTTCTGCTCCCATCAAACAGACCCTGACGCGCGCCGAACTGGGATCGTTCTATTCGGCGTTCACGGCCAACCCGACT
TACACCGAGCGGGTACTGACATTGGAGCCGGAGCGCTACAAGCGCCTGAGCGACATTGTCGTCAGTACCGGCTTTTTCTT
CCCAGCGTTGTTTACCCCGGCGGACCCGAAACTCCTCGCCAAACCCCTGGGAAGCCCCGAGAAGGCAGGAGCATTGCTGG
GTGAATACCTGAGACTCTACCGGCCGGACGCCGCGCAGGCCGAGTGGGAGCAGAGCGTGCGCGAACTGGCCCACGAAGCC
GGGGTCAAAGACGGCAAGCTCTTCATGACCCTCCGCGTCGCCGTCACCGGCCGCGAGCAGACACCGCCCCTGTTCGAGGT
GCTCACGATCCTGGGAGGAGACGAGGTGCAGCGCCGCCTAGAACTGGCTGCTGCGATGCCGGTGTCTCTGTAG

Upstream 100 bases:

>100_bases
CCCTCCAGTATCCTCCCGCTTTCGGCAGAGGTGCCTACCCGCTCCCCGGGCCCCACCGCCCGGGTATAAAATGTGACAGC
GCGCAACATTCGAGGGGCCG

Downstream 100 bases:

>100_bases
ACAGTGCCGGTGTTTGTTTCGCTAACGGCCCTTGCCCAGATAAAACGTCTCGATGCTGCGCTTGTCGCCCACGTAGCGCC
AGTGCCAGGGCTCGTAGCTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNFEQYESAYPPRALPPGAWVTRIGPSPTGKAHIGTALQAVINRSLADGSGGAFLLRIEDTDRERFVEGAIEDLLAALDW
LGIRPDESVAHGGAYGPYVQSERLPIYQAAARQLVEVGHAYYCFCTPERLESVRMAQQAAGKPTMYDRHCRNLGREEVEK
RLATGEKAVVRLKVPDGTEIAFDDLARGRIAFESQTIDDSVLLKSDGFPTYHLAVVVDDHLMRVNQIIRGEEWIPSTPKH
VLLYQYFGWPMPRIAHTPILRDMSRRKLSKRSGDTSITGYRVQGYLPEGLRNFLTRIIWPHPEGKDVYDHEEFVRLFKAE
DLPNTGPIVDPQLLDFINGQYLRRLSSAELYTATVEYLKFLLGLHQDIIFETYHPSAPIKQTLTRAELGSFYSAFTANPT
YTERVLTLEPERYKRLSDIVVSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEA
GVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELAAAMPVSL

Sequences:

>Translated_530_residues
MNFEQYESAYPPRALPPGAWVTRIGPSPTGKAHIGTALQAVINRSLADGSGGAFLLRIEDTDRERFVEGAIEDLLAALDW
LGIRPDESVAHGGAYGPYVQSERLPIYQAAARQLVEVGHAYYCFCTPERLESVRMAQQAAGKPTMYDRHCRNLGREEVEK
RLATGEKAVVRLKVPDGTEIAFDDLARGRIAFESQTIDDSVLLKSDGFPTYHLAVVVDDHLMRVNQIIRGEEWIPSTPKH
VLLYQYFGWPMPRIAHTPILRDMSRRKLSKRSGDTSITGYRVQGYLPEGLRNFLTRIIWPHPEGKDVYDHEEFVRLFKAE
DLPNTGPIVDPQLLDFINGQYLRRLSSAELYTATVEYLKFLLGLHQDIIFETYHPSAPIKQTLTRAELGSFYSAFTANPT
YTERVLTLEPERYKRLSDIVVSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEA
GVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELAAAMPVSL
>Mature_530_residues
MNFEQYESAYPPRALPPGAWVTRIGPSPTGKAHIGTALQAVINRSLADGSGGAFLLRIEDTDRERFVEGAIEDLLAALDW
LGIRPDESVAHGGAYGPYVQSERLPIYQAAARQLVEVGHAYYCFCTPERLESVRMAQQAAGKPTMYDRHCRNLGREEVEK
RLATGEKAVVRLKVPDGTEIAFDDLARGRIAFESQTIDDSVLLKSDGFPTYHLAVVVDDHLMRVNQIIRGEEWIPSTPKH
VLLYQYFGWPMPRIAHTPILRDMSRRKLSKRSGDTSITGYRVQGYLPEGLRNFLTRIIWPHPEGKDVYDHEEFVRLFKAE
DLPNTGPIVDPQLLDFINGQYLRRLSSAELYTATVEYLKFLLGLHQDIIFETYHPSAPIKQTLTRAELGSFYSAFTANPT
YTERVLTLEPERYKRLSDIVVSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEA
GVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELAAAMPVSL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=506, Percent_Identity=34.3873517786561, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1788743, Length=510, Percent_Identity=32.3529411764706, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI226510925, Length=254, Percent_Identity=34.6456692913386, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=510, Percent_Identity=31.5686274509804, Blast_Score=249, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6324540, Length=515, Percent_Identity=34.9514563106796, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24665321, Length=510, Percent_Identity=34.9019607843137, Blast_Score=251, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_GLOVI (Q7NI06)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925324.1
- ProteinModelPortal:   Q7NI06
- SMR:   Q7NI06
- GeneID:   2600779
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2378
- HOGENOM:   HBG628189
- OMA:   NELIRES
- BioCyc:   GVIO251221:GLL2378-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 59559; Mature: 59559

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEQYESAYPPRALPPGAWVTRIGPSPTGKAHIGTALQAVINRSLADGSGGAFLLRIED
CCHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
TDRERFVEGAIEDLLAALDWLGIRPDESVAHGGAYGPYVQSERLPIYQAAARQLVEVGHA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
YYCFCTPERLESVRMAQQAAGKPTMYDRHCRNLGREEVEKRLATGEKAVVRLKVPDGTEI
EEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCE
AFDDLARGRIAFESQTIDDSVLLKSDGFPTYHLAVVVDDHLMRVNQIIRGEEWIPSTPKH
EHHHHHCCEEEECCCCCCCCEEEECCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCCC
VLLYQYFGWPMPRIAHTPILRDMSRRKLSKRSGDTSITGYRVQGYLPEGLRNFLTRIIWP
EEEEHHHCCCCCHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCC
HPEGKDVYDHEEFVRLFKAEDLPNTGPIVDPQLLDFINGQYLRRLSSAELYTATVEYLKF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHHHH
LLGLHQDIIFETYHPSAPIKQTLTRAELGSFYSAFTANPTYTERVLTLEPERYKRLSDIV
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCEEEEECHHHHHHHHHHH
VSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEA
HHHCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELAAAMPVSL
CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNFEQYESAYPPRALPPGAWVTRIGPSPTGKAHIGTALQAVINRSLADGSGGAFLLRIED
CCHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
TDRERFVEGAIEDLLAALDWLGIRPDESVAHGGAYGPYVQSERLPIYQAAARQLVEVGHA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
YYCFCTPERLESVRMAQQAAGKPTMYDRHCRNLGREEVEKRLATGEKAVVRLKVPDGTEI
EEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCE
AFDDLARGRIAFESQTIDDSVLLKSDGFPTYHLAVVVDDHLMRVNQIIRGEEWIPSTPKH
EHHHHHCCEEEECCCCCCCCEEEECCCCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCCC
VLLYQYFGWPMPRIAHTPILRDMSRRKLSKRSGDTSITGYRVQGYLPEGLRNFLTRIIWP
EEEEHHHCCCCCHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCCC
HPEGKDVYDHEEFVRLFKAEDLPNTGPIVDPQLLDFINGQYLRRLSSAELYTATVEYLKF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHCCHHHHHHHHHHHH
LLGLHQDIIFETYHPSAPIKQTLTRAELGSFYSAFTANPTYTERVLTLEPERYKRLSDIV
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCEEEEECHHHHHHHHHHH
VSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEA
HHHCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELAAAMPVSL
CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292