Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 37521911

GI number: 37521911

Start: 2502220

End: 2503884

Strand: Reverse

Name: gatB

Synonym: gll2342

Alternate gene names: 37521911

Gene position: 2503884-2502220 (Counterclockwise)

Preceding gene: 37521915

Following gene: 37521910

Centisome position: 53.74

GC content: 63.18

Gene sequence:

>1665_bases
ATGACCAGCACGGCCCCACCCAAAGTCCAGTACGAAGCGGTGATCGGCCTGGAGGTGCATGTACAGCTCTCCACCGAGAC
GAAGCTATTCTGCCGGTGTTCGACCCGCTTCGGCAACACCCCCAACACGAACATCTGCCCGATCTGCACCGGCCAGCCGG
GCACCCTGCCGGTGCTCAACCAGCAGGCCCTCGACTACGCGGTGCTGACTGCCAGTGCCCTCAACTGCCAGATCCATCCC
CAGGGGCTGAGCAAGTTCGACCGCAAGCAGTACTTCTATCCGGACCTGCCCAAAAATTACCAGATCTCGCAGTACGACCT
GCCCCTGGCGGAGCGCGGCTGGCTTGAAATCGAGGTGGAGGGCGAACCGGCCAAACGCATCGGCATCACCCGGCTGCACA
TGGAGGAGGACGCGGGCAAGCTCGTGCACGCCGGGGCAGATCGCCTCTCGGGTTCGACCCATTCGCTGGTCGACTTTAAC
CGCGCCGGGGTGGCGTTGTGCGAGATCGTCTCGGAGCCGGACATCCGCACAGCCGCCGAGGCTGCCGCCTACGCCGGGGA
GTTGCGCCGCATCGTGCGTTATCTCGGGGTGTGCGACGGCAACATGCAGGAGGGCTCGCTGCGCTTCGATCTGAACATTT
CGGTGCGGCCCGCAGGCGAGGGCAAATTCGGCACCAAAGTCGAAATCAAAAACCTCAACAGCTTCAACTCGCTGCAGCGG
GCGGTGGAGTACGAATTTGCCCGCCAGGTCGATTGCCTCCTCTCAGGCGAGCGCATCGTGCAGGAGACCCGGCTGTGGGA
CGAAGCCACCCAGCGCACCATCTCGATGCGCTCCAAAGAAGAAGCGAACGACTACCGCTACTTCCCGGAACCGGATCTCG
TGCCGATTGCACTAAATGGCACTCAGATCGACGCTTACCGGCAGCGGCTTGGCGAACTTCCCGCACAAAAACGCCATCGC
TACCGCGAGACGCTCGGATTGTCGTCCTACGACGCGGGCGTGCTGACCGACGAGCGCGAGGTGGCCGAATATTTCGAGCA
AGTAGTGGCCCTGGGGATACCTGCCAAGCAGGCGGCCAACTTCGTCAGCGGGGCGGTGGCCGCCCACCTCAACGAGACGC
GCCGGTCGATTTCGCAGATCAAGGTCACTCCCGAGGTTTCGGCCGAATTGCTTGCGCTCATCAACCAGGGTATTATTAGC
AACCGGATAGCTAACGAGCTTCTACCCGACCTGTTCGAGAAAGGGGGCTCCCCACGCGCCCTCGTCGAAGAGCGCGGTCT
GACACAAATCAGTGACAGGGGGCAGCTTGAACAGATCGTGGATGAGGTGCTGGCTGGAGAATCCGATTCGGTGGCTGCCT
ACCGGGGTGGACGCACCAAATTACTCGGATTCTTCGTCGGCAAGGTGATGAAGAAGACGGCCGGCCGCGCCGACCCGCAG
GTGGTCAACGATCTATTGCAAAGCAAGCTTGCCGAGCAACCGACTGCACCGCCCCCGGAGCCGGAGTCTGCCGCCGAGAC
CCCCGAGGCGCCCCCCGCTGTCGAGGATGCCCCACCCGAGGCACCCACCGAAGCGATCACCGCCGAGGCCGGGTCGGCAG
AAGCCATCACCGCCGCTTCCGAAGAGCCGGACACGCCCGTTAGTCACCAAGACGCCCACGCATAA

Upstream 100 bases:

>100_bases
TGAAATCGGGTGAGGACCAGTTGCCAGCTGCCGTTCTCCATGCTTCCCTTGTACCCTCTATGCCAAAATTGAGAACGACG
AACGGTTGAATGCAAGCTTT

Downstream 100 bases:

>100_bases
TGCATGGAGAACCTCCGAAATGATCTCATGGTCTAAATTTGCAGCTTGCTCGCTGGCTGCCCTGGTGGCGGTCTGCGCCA
CTGGCCTGGCTGCCGGTGCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTSTAPPKVQYEAVIGLEVHVQLSTETKLFCRCSTRFGNTPNTNICPICTGQPGTLPVLNQQALDYAVLTASALNCQIHP
QGLSKFDRKQYFYPDLPKNYQISQYDLPLAERGWLEIEVEGEPAKRIGITRLHMEEDAGKLVHAGADRLSGSTHSLVDFN
RAGVALCEIVSEPDIRTAAEAAAYAGELRRIVRYLGVCDGNMQEGSLRFDLNISVRPAGEGKFGTKVEIKNLNSFNSLQR
AVEYEFARQVDCLLSGERIVQETRLWDEATQRTISMRSKEEANDYRYFPEPDLVPIALNGTQIDAYRQRLGELPAQKRHR
YRETLGLSSYDAGVLTDEREVAEYFEQVVALGIPAKQAANFVSGAVAAHLNETRRSISQIKVTPEVSAELLALINQGIIS
NRIANELLPDLFEKGGSPRALVEERGLTQISDRGQLEQIVDEVLAGESDSVAAYRGGRTKLLGFFVGKVMKKTAGRADPQ
VVNDLLQSKLAEQPTAPPPEPESAAETPEAPPAVEDAPPEAPTEAITAEAGSAEAITAASEEPDTPVSHQDAHA

Sequences:

>Translated_554_residues
MTSTAPPKVQYEAVIGLEVHVQLSTETKLFCRCSTRFGNTPNTNICPICTGQPGTLPVLNQQALDYAVLTASALNCQIHP
QGLSKFDRKQYFYPDLPKNYQISQYDLPLAERGWLEIEVEGEPAKRIGITRLHMEEDAGKLVHAGADRLSGSTHSLVDFN
RAGVALCEIVSEPDIRTAAEAAAYAGELRRIVRYLGVCDGNMQEGSLRFDLNISVRPAGEGKFGTKVEIKNLNSFNSLQR
AVEYEFARQVDCLLSGERIVQETRLWDEATQRTISMRSKEEANDYRYFPEPDLVPIALNGTQIDAYRQRLGELPAQKRHR
YRETLGLSSYDAGVLTDEREVAEYFEQVVALGIPAKQAANFVSGAVAAHLNETRRSISQIKVTPEVSAELLALINQGIIS
NRIANELLPDLFEKGGSPRALVEERGLTQISDRGQLEQIVDEVLAGESDSVAAYRGGRTKLLGFFVGKVMKKTAGRADPQ
VVNDLLQSKLAEQPTAPPPEPESAAETPEAPPAVEDAPPEAPTEAITAEAGSAEAITAASEEPDTPVSHQDAHA
>Mature_553_residues
TSTAPPKVQYEAVIGLEVHVQLSTETKLFCRCSTRFGNTPNTNICPICTGQPGTLPVLNQQALDYAVLTASALNCQIHPQ
GLSKFDRKQYFYPDLPKNYQISQYDLPLAERGWLEIEVEGEPAKRIGITRLHMEEDAGKLVHAGADRLSGSTHSLVDFNR
AGVALCEIVSEPDIRTAAEAAAYAGELRRIVRYLGVCDGNMQEGSLRFDLNISVRPAGEGKFGTKVEIKNLNSFNSLQRA
VEYEFARQVDCLLSGERIVQETRLWDEATQRTISMRSKEEANDYRYFPEPDLVPIALNGTQIDAYRQRLGELPAQKRHRY
RETLGLSSYDAGVLTDEREVAEYFEQVVALGIPAKQAANFVSGAVAAHLNETRRSISQIKVTPEVSAELLALINQGIISN
RIANELLPDLFEKGGSPRALVEERGLTQISDRGQLEQIVDEVLAGESDSVAAYRGGRTKLLGFFVGKVMKKTAGRADPQV
VNDLLQSKLAEQPTAPPPEPESAAETPEAPPAVEDAPPEAPTEAITAEAGSAEAITAASEEPDTPVSHQDAHA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=503, Percent_Identity=36.5805168986084, Blast_Score=292, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI115534094, Length=358, Percent_Identity=37.1508379888268, Blast_Score=224, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=516, Percent_Identity=29.6511627906977, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45550808, Length=518, Percent_Identity=33.3976833976834, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_GLOVI (Q7NI42)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925288.1
- ProteinModelPortal:   Q7NI42
- SMR:   Q7NI42
- GeneID:   2600743
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2342
- NMPDR:   fig|251221.1.peg.2342
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   GVIO251221:GLL2342-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 60534; Mature: 60402

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTAPPKVQYEAVIGLEVHVQLSTETKLFCRCSTRFGNTPNTNICPICTGQPGTLPVLN
CCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCC
QQALDYAVLTASALNCQIHPQGLSKFDRKQYFYPDLPKNYQISQYDLPLAERGWLEIEVE
HHHHHHHHHHHHHHCEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
GEPAKRIGITRLHMEEDAGKLVHAGADRLSGSTHSLVDFNRAGVALCEIVSEPDIRTAAE
CCCHHHHCCEEEEEHHCCCHHHHCCHHHCCCCCCHHEECCCCCHHHHHHHCCCCHHHHHH
AAAYAGELRRIVRYLGVCDGNMQEGSLRFDLNISVRPAGEGKFGTKVEIKNLNSFNSLQR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHH
AVEYEFARQVDCLLSGERIVQETRLWDEATQRTISMRSKEEANDYRYFPEPDLVPIALNG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
TQIDAYRQRLGELPAQKRHRYRETLGLSSYDAGVLTDEREVAEYFEQVVALGIPAKQAAN
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
FVSGAVAAHLNETRRSISQIKVTPEVSAELLALINQGIISNRIANELLPDLFEKGGSPRA
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LVEERGLTQISDRGQLEQIVDEVLAGESDSVAAYRGGRTKLLGFFVGKVMKKTAGRADPQ
HHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
VVNDLLQSKLAEQPTAPPPEPESAAETPEAPPAVEDAPPEAPTEAITAEAGSAEAITAAS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECC
EEPDTPVSHQDAHA
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TSTAPPKVQYEAVIGLEVHVQLSTETKLFCRCSTRFGNTPNTNICPICTGQPGTLPVLN
CCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCC
QQALDYAVLTASALNCQIHPQGLSKFDRKQYFYPDLPKNYQISQYDLPLAERGWLEIEVE
HHHHHHHHHHHHHHCEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
GEPAKRIGITRLHMEEDAGKLVHAGADRLSGSTHSLVDFNRAGVALCEIVSEPDIRTAAE
CCCHHHHCCEEEEEHHCCCHHHHCCHHHCCCCCCHHEECCCCCHHHHHHHCCCCHHHHHH
AAAYAGELRRIVRYLGVCDGNMQEGSLRFDLNISVRPAGEGKFGTKVEIKNLNSFNSLQR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHH
AVEYEFARQVDCLLSGERIVQETRLWDEATQRTISMRSKEEANDYRYFPEPDLVPIALNG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
TQIDAYRQRLGELPAQKRHRYRETLGLSSYDAGVLTDEREVAEYFEQVVALGIPAKQAAN
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
FVSGAVAAHLNETRRSISQIKVTPEVSAELLALINQGIISNRIANELLPDLFEKGGSPRA
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LVEERGLTQISDRGQLEQIVDEVLAGESDSVAAYRGGRTKLLGFFVGKVMKKTAGRADPQ
HHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
VVNDLLQSKLAEQPTAPPPEPESAAETPEAPPAVEDAPPEAPTEAITAEAGSAEAITAAS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECC
EEPDTPVSHQDAHA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292