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The map label for this gene is cysW [H]

Identifier: 37521643

GI number: 37521643

Start: 2226700

End: 2227569

Strand: Direct

Name: cysW [H]

Synonym: glr2074

Alternate gene names: 37521643

Gene position: 2226700-2227569 (Clockwise)

Preceding gene: 37521642

Following gene: 37521644

Centisome position: 47.79

GC content: 61.72

Gene sequence:

>870_bases
ATGGTTGCTGTCATCGAAAAACGCCCGCAAAGGCGGGAATTCTGGGAGCGAGCCACTACCGAACCTGCCTGGCTGCGCTG
GGTGCTGGTTGCAGTGGCGCTCGCCTTCTGCGGATTGTTCCTGGTGGTACCGCTGGTGGCGGTCTTTGCCCAGGCCCTCG
AAAAAGGTTGGGGACTGTATCTGGCTTCCTTCGCCGACGCCGACGCCCTGACGGCCATTCAACTGACGCTCCTGGTCAGC
GCCATCGCCGTGCCCGTCAACCTGGTCTTCGGTGTCGCCGCCGCCTGGGCGATTGCCAAGTTCGATTTCAAGGGCAAGAA
CTTTTTGACCACGTTGGTGGATTTGCCCTTCTCGGTATCGCCCGTGGTCTCGGGGTTGATTTTCGTGCTGCTGTTCGGGG
CGCAGGGCTGGTTTGGCCCCTGGCTTTCGGCCAACGACTTCAAAATCATCTTCGCGGTGCCGGGAATTGTGCTGGCGACC
GTGTTCGTCACCTTTCCGTTTATCGCCCGCGAACTGATTCCGCTGATGCAGGAGCAGGGAACAGAGCAGGAGGAGACGGC
CCTGGTACTCGGTGCAAGCGGCTGGCAGGCCTTCTGGCGGGTCACCCTGCCCAATATCAAGTGGGCTTTGCTTTACGGGG
TGATCCTCTGCAACGCCCGGGCGATGGGCGAATTCGGCGCCGTCTCGGTGGTCTCGGGCCACATCCGCGGACAAACCAAC
ACGATGCCCCTGCACGTTGAGATCCTCTACAACGAGTACAACTTCGTGGGCGCCTTCGCGGTCGCCTCGCTGCTGGCACT
GCTGGCGCTGGTCACCCTGGTGCTCAAGAGCCTCGTCGAGTGGAAATCGGCCCACCCAACCGAGCGTTGA

Upstream 100 bases:

>100_bases
GGTGATGCTGGTGGCGGCCTTCGTACTGCTCTTTGCCATCAATTTGATCCAGCGCTGGAGCAGCCGCTTCGCCGCCCGCT
AACCCGCAGGAGGATATCCT

Downstream 100 bases:

>100_bases
TGGCCGGGTCCATTGCCCAGCTGAAGGCCGCTTTGGTAACGCTCGCCGCCCTACTTGGGCTCGGCTTGCTCCACACCCCG
TCTGTCCTCGCCCAGTCCGC

Product: sulfate ABC transporter permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MVAVIEKRPQRREFWERATTEPAWLRWVLVAVALAFCGLFLVVPLVAVFAQALEKGWGLYLASFADADALTAIQLTLLVS
AIAVPVNLVFGVAAAWAIAKFDFKGKNFLTTLVDLPFSVSPVVSGLIFVLLFGAQGWFGPWLSANDFKIIFAVPGIVLAT
VFVTFPFIARELIPLMQEQGTEQEETALVLGASGWQAFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSGHIRGQTN
TMPLHVEILYNEYNFVGAFAVASLLALLALVTLVLKSLVEWKSAHPTER

Sequences:

>Translated_289_residues
MVAVIEKRPQRREFWERATTEPAWLRWVLVAVALAFCGLFLVVPLVAVFAQALEKGWGLYLASFADADALTAIQLTLLVS
AIAVPVNLVFGVAAAWAIAKFDFKGKNFLTTLVDLPFSVSPVVSGLIFVLLFGAQGWFGPWLSANDFKIIFAVPGIVLAT
VFVTFPFIARELIPLMQEQGTEQEETALVLGASGWQAFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSGHIRGQTN
TMPLHVEILYNEYNFVGAFAVASLLALLALVTLVLKSLVEWKSAHPTER
>Mature_289_residues
MVAVIEKRPQRREFWERATTEPAWLRWVLVAVALAFCGLFLVVPLVAVFAQALEKGWGLYLASFADADALTAIQLTLLVS
AIAVPVNLVFGVAAAWAIAKFDFKGKNFLTTLVDLPFSVSPVVSGLIFVLLFGAQGWFGPWLSANDFKIIFAVPGIVLAT
VFVTFPFIARELIPLMQEQGTEQEETALVLGASGWQAFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSGHIRGQTN
TMPLHVEILYNEYNFVGAFAVASLLALLALVTLVLKSLVEWKSAHPTER

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=275, Percent_Identity=50.9090909090909, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788764, Length=215, Percent_Identity=35.8139534883721, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=31.6742081447964, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787714, Length=218, Percent_Identity=30.7339449541284, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31654; Mature: 31654

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIEKRPQRREFWERATTEPAWLRWVLVAVALAFCGLFLVVPLVAVFAQALEKGWGLY
CEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LASFADADALTAIQLTLLVSAIAVPVNLVFGVAAAWAIAKFDFKGKNFLTTLVDLPFSVS
EHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHH
PVVSGLIFVLLFGAQGWFGPWLSANDFKIIFAVPGIVLATVFVTFPFIARELIPLMQEQG
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEQEETALVLGASGWQAFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSGHIRGQTN
CCCHHCEEEEECCCCHHEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHCCCCCCC
TMPLHVEILYNEYNFVGAFAVASLLALLALVTLVLKSLVEWKSAHPTER
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVAVIEKRPQRREFWERATTEPAWLRWVLVAVALAFCGLFLVVPLVAVFAQALEKGWGLY
CEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LASFADADALTAIQLTLLVSAIAVPVNLVFGVAAAWAIAKFDFKGKNFLTTLVDLPFSVS
EHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHH
PVVSGLIFVLLFGAQGWFGPWLSANDFKIIFAVPGIVLATVFVTFPFIARELIPLMQEQG
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEQEETALVLGASGWQAFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSGHIRGQTN
CCCHHCEEEEECCCCHHEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHCCCCCCC
TMPLHVEILYNEYNFVGAFAVASLLALLALVTLVLKSLVEWKSAHPTER
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]