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The map label for this gene is cca [H]

Identifier: 37521022

GI number: 37521022

Start: 1558732

End: 1560480

Strand: Reverse

Name: cca [H]

Synonym: gll1453

Alternate gene names: 37521022

Gene position: 1560480-1558732 (Counterclockwise)

Preceding gene: 37521023

Following gene: 37521021

Centisome position: 33.49

GC content: 67.41

Gene sequence:

>1749_bases
GTGGGCGGACGCCGAACTCGACCGCACCCCCCGCTCGGGCGATTCCCCGAAAGAAAAACGGCGGTGAGCGCGGTGGTGCT
GGCCCGCGACGTGATGTCCGCGCCCGTGCGCACACTGCGCCCGGAGATCGCTTTGATGGCGGCCCGGCAGCGCCTGACCC
GCTACGGGCACGGGGCGATGCCGGTGGTGAACGCGCAGGGCGGTCTGATGGGAATCATCTCGCGTCGCGATCTCGATATT
GCTCTTTATCACGGATTCGCCGAGGATCCGGTGGAGCGGTTCATGACCGCGACGGTGCTCACGGTGCCCTCGGACGCCCC
GCTCGATGAGATCGAAGCGCTGATGGTCACCTACGACATCGGCCGGGTGCCGGTACTGGAGGCGGGGCAACTGGTGGGCA
TCATCACCCGCACCGACCTGCTGCGCCAGCACCACCAGCAGCGCACCGGCGGGCTGCCGCTGTTCCTGCCTTTGCAGGAG
CGTCTTGAAGCCAAACTCGAAGCGGTGGCCATCGAGCGCCTCGCCCGGGTGGCCGCCACCGCCGAGGCGCTCGATCAGGT
GGCATATCTGGTGGGCGGTGCGGTGCGCGACTTGCTGCTGGGTGAAACCAGCAGCGATCTCGATGTCGTGGTCGAAGGCC
GCGGGGGCGAAGCCGGGGCCGCCCCGGCCCTGGCGCGCGCCATCGCCGCAGAGGACGCAACTTTGCGCCTGGAGTGCTTC
GAGAAGTACCAGACCGCCGCGCTGATCTGGCCCGATGGCTTCGTGCTGGATCTGGCCACCGCCAGGACCGAGTTCTATCC
CAGACCGGGGGCCAACCCCGAAGTCGAGAGCGCCTCGATCCGCCTCGATCTGTACCGTCGGGATTTTACGATCAACGCGA
TGGCCGTCTGCCTCAACGGCCCACGCGCCTGGCAACTGATTGACTTTTTCGGCGGATTTGTCGATCTGCGCGAGCGCATC
GTGCGGGTGCTGCACCCCAACAGCTTCATCGAAGACCCGACCCGGATCTTTCGGGCGGTGCGCTTTGCGCTACGGCTCGG
CTTCGCGATCGCCCCCCAGACCGAGGCGTTTATTGTGCGCAGCGTTTCGGGCGGTCTGCACGATCTTTACGGCGGGGAAC
GCCTGAAGACGGAACTCGGCTACTTGCTTGCGGCCCCTTTTTGGTTGGAAGGGCTGCAACTGCTCGACCGGCTCGGGGCG
CTGCGCTGCGTGCACCCGGATCTGCGACTGACGGAGGCCTTGGCAGCGGCGTTGGTGTGGCTGGAGGGTGGGTATGGAGC
GTTTGCGCCTTTGCGGGCGACCCTCCCTCAGTTGCGGCTGGATCGGCTGCTGCTCGCCGTGGGGCCGCAGGCGGCCGAGA
GCCTCCCCCTCGACGGCCACGCCCGTACCCGGCAGCGCAAAGTGCGCGCCGCGCTCCTCGTCCTCGCCAAACAGACCGAT
TGGTCCGCCGCCGCGCTCCACCGGCACCTGGCGGGCCTTGGGCCGGAGGAGAGTCTGCTGCTGGCAAGTGCGATCGGGCA
GACCGAACGGCGCCAGTCGATCGCTGATTATCTGCAGCGCCGCCGGGAACCGCCGCTGGTGGGCGGCGACGACCTGAAAG
CCCTGGGTCTCAAGCCCGGTCCGGACTTTCGCAAAATTCTCGATGACGCCACAACGGCCCAACTTGCCGGCAAGCTCGTC
GACCGCGAAGGAGCGCTTGCCTGGCTGCAAGACCGCGCCCAACCGGGAAATCGCGCCTACGAGAATTAA

Upstream 100 bases:

>100_bases
GCGGCCACGATCTGTGCGAGGAAATCGAGCGCGCCGTCGAGGCGGCCTTGCCCCAGAGTTATGTCACCACCCACCTCGAA
CCGCTTGAAGATCCGCTCTC

Downstream 100 bases:

>100_bases
TTGATAATGATAATCGCTCCTGCTATCATCGCCACGATGGTTGAGCGAGGAGTGAGCGGTGGTGCCGATCTGGAATTTTT
ATGGGCTGCATCGGGCAAGC

Product: poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MGGRRTRPHPPLGRFPERKTAVSAVVLARDVMSAPVRTLRPEIALMAARQRLTRYGHGAMPVVNAQGGLMGIISRRDLDI
ALYHGFAEDPVERFMTATVLTVPSDAPLDEIEALMVTYDIGRVPVLEAGQLVGIITRTDLLRQHHQQRTGGLPLFLPLQE
RLEAKLEAVAIERLARVAATAEALDQVAYLVGGAVRDLLLGETSSDLDVVVEGRGGEAGAAPALARAIAAEDATLRLECF
EKYQTAALIWPDGFVLDLATARTEFYPRPGANPEVESASIRLDLYRRDFTINAMAVCLNGPRAWQLIDFFGGFVDLRERI
VRVLHPNSFIEDPTRIFRAVRFALRLGFAIAPQTEAFIVRSVSGGLHDLYGGERLKTELGYLLAAPFWLEGLQLLDRLGA
LRCVHPDLRLTEALAAALVWLEGGYGAFAPLRATLPQLRLDRLLLAVGPQAAESLPLDGHARTRQRKVRAALLVLAKQTD
WSAAALHRHLAGLGPEESLLLASAIGQTERRQSIADYLQRRREPPLVGGDDLKALGLKPGPDFRKILDDATTAQLAGKLV
DREGALAWLQDRAQPGNRAYEN

Sequences:

>Translated_582_residues
MGGRRTRPHPPLGRFPERKTAVSAVVLARDVMSAPVRTLRPEIALMAARQRLTRYGHGAMPVVNAQGGLMGIISRRDLDI
ALYHGFAEDPVERFMTATVLTVPSDAPLDEIEALMVTYDIGRVPVLEAGQLVGIITRTDLLRQHHQQRTGGLPLFLPLQE
RLEAKLEAVAIERLARVAATAEALDQVAYLVGGAVRDLLLGETSSDLDVVVEGRGGEAGAAPALARAIAAEDATLRLECF
EKYQTAALIWPDGFVLDLATARTEFYPRPGANPEVESASIRLDLYRRDFTINAMAVCLNGPRAWQLIDFFGGFVDLRERI
VRVLHPNSFIEDPTRIFRAVRFALRLGFAIAPQTEAFIVRSVSGGLHDLYGGERLKTELGYLLAAPFWLEGLQLLDRLGA
LRCVHPDLRLTEALAAALVWLEGGYGAFAPLRATLPQLRLDRLLLAVGPQAAESLPLDGHARTRQRKVRAALLVLAKQTD
WSAAALHRHLAGLGPEESLLLASAIGQTERRQSIADYLQRRREPPLVGGDDLKALGLKPGPDFRKILDDATTAQLAGKLV
DREGALAWLQDRAQPGNRAYEN
>Mature_581_residues
GGRRTRPHPPLGRFPERKTAVSAVVLARDVMSAPVRTLRPEIALMAARQRLTRYGHGAMPVVNAQGGLMGIISRRDLDIA
LYHGFAEDPVERFMTATVLTVPSDAPLDEIEALMVTYDIGRVPVLEAGQLVGIITRTDLLRQHHQQRTGGLPLFLPLQER
LEAKLEAVAIERLARVAATAEALDQVAYLVGGAVRDLLLGETSSDLDVVVEGRGGEAGAAPALARAIAAEDATLRLECFE
KYQTAALIWPDGFVLDLATARTEFYPRPGANPEVESASIRLDLYRRDFTINAMAVCLNGPRAWQLIDFFGGFVDLRERIV
RVLHPNSFIEDPTRIFRAVRFALRLGFAIAPQTEAFIVRSVSGGLHDLYGGERLKTELGYLLAAPFWLEGLQLLDRLGAL
RCVHPDLRLTEALAAALVWLEGGYGAFAPLRATLPQLRLDRLLLAVGPQAAESLPLDGHARTRQRKVRAALLVLAKQTDW
SAAALHRHLAGLGPEESLLLASAIGQTERRQSIADYLQRRREPPLVGGDDLKALGLKPGPDFRKILDDATTAQLAGKLVD
REGALAWLQDRAQPGNRAYEN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=242, Percent_Identity=33.4710743801653, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=28.82096069869, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 63699; Mature: 63568

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGRRTRPHPPLGRFPERKTAVSAVVLARDVMSAPVRTLRPEIALMAARQRLTRYGHGAM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHCCCCCC
PVVNAQGGLMGIISRRDLDIALYHGFAEDPVERFMTATVLTVPSDAPLDEIEALMVTYDI
CEEECCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHEEEEC
GRVPVLEAGQLVGIITRTDLLRQHHQQRTGGLPLFLPLQERLEAKLEAVAIERLARVAAT
CCCCEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
AEALDQVAYLVGGAVRDLLLGETSSDLDVVVEGRGGEAGAAPALARAIAAEDATLRLECF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHHH
EKYQTAALIWPDGFVLDLATARTEFYPRPGANPEVESASIRLDLYRRDFTINAMAVCLNG
HHHCCEEEECCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEECC
PRAWQLIDFFGGFVDLRERIVRVLHPNSFIEDPTRIFRAVRFALRLGFAIAPQTEAFIVR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEEEE
SVSGGLHDLYGGERLKTELGYLLAAPFWLEGLQLLDRLGALRCVHPDLRLTEALAAALVW
ECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
LEGGYGAFAPLRATLPQLRLDRLLLAVGPQAAESLPLDGHARTRQRKVRAALLVLAKQTD
EECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
WSAAALHRHLAGLGPEESLLLASAIGQTERRQSIADYLQRRREPPLVGGDDLKALGLKPG
CHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PDFRKILDDATTAQLAGKLVDREGALAWLQDRAQPGNRAYEN
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GGRRTRPHPPLGRFPERKTAVSAVVLARDVMSAPVRTLRPEIALMAARQRLTRYGHGAM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHCCCCCC
PVVNAQGGLMGIISRRDLDIALYHGFAEDPVERFMTATVLTVPSDAPLDEIEALMVTYDI
CEEECCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHEEEEC
GRVPVLEAGQLVGIITRTDLLRQHHQQRTGGLPLFLPLQERLEAKLEAVAIERLARVAAT
CCCCEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
AEALDQVAYLVGGAVRDLLLGETSSDLDVVVEGRGGEAGAAPALARAIAAEDATLRLECF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHHH
EKYQTAALIWPDGFVLDLATARTEFYPRPGANPEVESASIRLDLYRRDFTINAMAVCLNG
HHHCCEEEECCCCEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEECC
PRAWQLIDFFGGFVDLRERIVRVLHPNSFIEDPTRIFRAVRFALRLGFAIAPQTEAFIVR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEEEE
SVSGGLHDLYGGERLKTELGYLLAAPFWLEGLQLLDRLGALRCVHPDLRLTEALAAALVW
ECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
LEGGYGAFAPLRATLPQLRLDRLLLAVGPQAAESLPLDGHARTRQRKVRAALLVLAKQTD
EECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
WSAAALHRHLAGLGPEESLLLASAIGQTERRQSIADYLQRRREPPLVGGDDLKALGLKPG
CHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PDFRKILDDATTAQLAGKLVDREGALAWLQDRAQPGNRAYEN
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]