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The map label for this gene is amiA [C]

Identifier: 37520932

GI number: 37520932

Start: 1456686

End: 1457891

Strand: Reverse

Name: amiA [C]

Synonym: gll1363

Alternate gene names: 37520932

Gene position: 1457891-1456686 (Counterclockwise)

Preceding gene: 37520946

Following gene: 37520931

Centisome position: 31.29

GC content: 56.3

Gene sequence:

>1206_bases
ATGGGATCAGGCGAAAATGATACTCTCTTGCAACCCGAGCCAGAGGATATTACCTTGGCAATTCCGACCAACTTGAGCGC
CCTTTTCAAGGATCTTGTTTTACTCTATGCCCGCACCGAGATTCAGTATCCTCAACTCAAAGCAGTGACGCTCGCGCAGT
GGATTTTGGAGTCGGGTTGGGGAGAATCGAATCTGGCCAAAAATCACTATAACTTCGGTGGACTCAAATGGCGCACAGAA
ATGACCGGTTTCGCCACCAGGATCCTTTATCAAGCGAACGATGGACCCGATTATTATTGTCAGTTCGCCGGCTTGGAGCG
CTTCATTCGGGGATACTGGCGATTTATCGATCGCTCACCCTACACAGGTTGGGAAGAGCGCACCGATAGTGGCGAAGAGT
TTATTCGCTTTGTCGGCCCGGTATACTCCCCGTCCCCAGGCTACGTAGACCGGGTGCTGGCGCTGGTGGATGAAGCACAG
ATCCTCCTTGACAACGCTGCTGAGCCGGTGATCGCGCCGATAGAAAGTCCGTCGCTACGGGGAACGGTAGTTCTCGATCC
TGGCCATGGGGGTACCCGCAAAATCGGGGGCAGTTCTCCCAACAATGCCATCAGTGCAAGCGGCATCAAAGAAAAGACTA
TGACCCTGGAAATAGCTCTGCTCATCCGCGACGCGCTGCAACAAATCGCCGCCCAAAACGGCTATGACCTTCGTGTCGTT
CTAACCCGTACCGCCGATGTCAATGTCGGTATTGCCGAGCGGGCCAACCTCGCAAGCGTGGAGAGGGCGGATCTGTTCTT
GAGCATTCATTTCAACGGCTTTAACAAACAGACCAGCGGGGTAGAAACCTACATTCGCGCCCGTACCAACGGCAATGTAA
ATTTCGAGGAAGACGAAGCGTTCGCGTGGCGCATTCAGGGGGCCGTGCTTGGGGCTTTGCGGACCTACCTCCCCGGCACG
CGCGATCGTGGCGTCAAAGAAGACACGGCGTCGGGGGGCGGAGTTTTGGGTGTCCTCGACGATCGCGCGTTGGGGAACTC
GTCACAGGAGTATCCTTGCCGGGCCTGCCTGGTGGAAATTGAATTTATCGACGTGCCCCGGGTGGATCGGTTGCTCAATA
CCGATGCGCAGGCCCGCCAGGTACGCGGGGCGATTGCTGAGGCAATTGCCGCCGCGATGGTCGAAGATTTGCAGGCCGCC
CGCTAA

Upstream 100 bases:

>100_bases
ATCGGGTGCCGGCTGGGGACTGGGACCATTGTGCCATCCCAGTCGCAATCTCCTTGCCCAAGGTTGGTCGGCACTGCATA
ATCCGGCACAGAATTTGTAT

Downstream 100 bases:

>100_bases
ATTTAAAATTCCCTCGTTCAAGGAACCGCACCATGCGCCAGATCGCTTTGCAGTTCGACAGCAATCCAAAAGATGTGCAC
GAAGACCTCTCTGCCGTGTG

Product: hypothetical protein

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: Cell Wall Hydrolase/Autolysin; N-Acetylmuramoyl-L-Alanine Amidase Domain Protein

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGSGENDTLLQPEPEDITLAIPTNLSALFKDLVLLYARTEIQYPQLKAVTLAQWILESGWGESNLAKNHYNFGGLKWRTE
MTGFATRILYQANDGPDYYCQFAGLERFIRGYWRFIDRSPYTGWEERTDSGEEFIRFVGPVYSPSPGYVDRVLALVDEAQ
ILLDNAAEPVIAPIESPSLRGTVVLDPGHGGTRKIGGSSPNNAISASGIKEKTMTLEIALLIRDALQQIAAQNGYDLRVV
LTRTADVNVGIAERANLASVERADLFLSIHFNGFNKQTSGVETYIRARTNGNVNFEEDEAFAWRIQGAVLGALRTYLPGT
RDRGVKEDTASGGGVLGVLDDRALGNSSQEYPCRACLVEIEFIDVPRVDRLLNTDAQARQVRGAIAEAIAAAMVEDLQAA
R

Sequences:

>Translated_401_residues
MGSGENDTLLQPEPEDITLAIPTNLSALFKDLVLLYARTEIQYPQLKAVTLAQWILESGWGESNLAKNHYNFGGLKWRTE
MTGFATRILYQANDGPDYYCQFAGLERFIRGYWRFIDRSPYTGWEERTDSGEEFIRFVGPVYSPSPGYVDRVLALVDEAQ
ILLDNAAEPVIAPIESPSLRGTVVLDPGHGGTRKIGGSSPNNAISASGIKEKTMTLEIALLIRDALQQIAAQNGYDLRVV
LTRTADVNVGIAERANLASVERADLFLSIHFNGFNKQTSGVETYIRARTNGNVNFEEDEAFAWRIQGAVLGALRTYLPGT
RDRGVKEDTASGGGVLGVLDDRALGNSSQEYPCRACLVEIEFIDVPRVDRLLNTDAQARQVRGAIAEAIAAAMVEDLQAA
R
>Mature_400_residues
GSGENDTLLQPEPEDITLAIPTNLSALFKDLVLLYARTEIQYPQLKAVTLAQWILESGWGESNLAKNHYNFGGLKWRTEM
TGFATRILYQANDGPDYYCQFAGLERFIRGYWRFIDRSPYTGWEERTDSGEEFIRFVGPVYSPSPGYVDRVLALVDEAQI
LLDNAAEPVIAPIESPSLRGTVVLDPGHGGTRKIGGSSPNNAISASGIKEKTMTLEIALLIRDALQQIAAQNGYDLRVVL
TRTADVNVGIAERANLASVERADLFLSIHFNGFNKQTSGVETYIRARTNGNVNFEEDEAFAWRIQGAVLGALRTYLPGTR
DRGVKEDTASGGGVLGVLDDRALGNSSQEYPCRACLVEIEFIDVPRVDRLLNTDAQARQVRGAIAEAIAAAMVEDLQAAR

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 44051; Mature: 43920

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGENDTLLQPEPEDITLAIPTNLSALFKDLVLLYARTEIQYPQLKAVTLAQWILESGW
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GESNLAKNHYNFGGLKWRTEMTGFATRILYQANDGPDYYCQFAGLERFIRGYWRFIDRSP
CCCCCCCCCCCCCCEEEEEHHHHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCC
YTGWEERTDSGEEFIRFVGPVYSPSPGYVDRVLALVDEAQILLDNAAEPVIAPIESPSLR
CCCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
GTVVLDPGHGGTRKIGGSSPNNAISASGIKEKTMTLEIALLIRDALQQIAAQNGYDLRVV
EEEEEECCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LTRTADVNVGIAERANLASVERADLFLSIHFNGFNKQTSGVETYIRARTNGNVNFEEDEA
EEECCCCCCCHHCCCCCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCE
FAWRIQGAVLGALRTYLPGTRDRGVKEDTASGGGVLGVLDDRALGNSSQEYPCRACLVEI
EEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEE
EFIDVPRVDRLLNTDAQARQVRGAIAEAIAAAMVEDLQAAR
EEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GSGENDTLLQPEPEDITLAIPTNLSALFKDLVLLYARTEIQYPQLKAVTLAQWILESGW
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GESNLAKNHYNFGGLKWRTEMTGFATRILYQANDGPDYYCQFAGLERFIRGYWRFIDRSP
CCCCCCCCCCCCCCEEEEEHHHHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCC
YTGWEERTDSGEEFIRFVGPVYSPSPGYVDRVLALVDEAQILLDNAAEPVIAPIESPSLR
CCCCHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
GTVVLDPGHGGTRKIGGSSPNNAISASGIKEKTMTLEIALLIRDALQQIAAQNGYDLRVV
EEEEEECCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LTRTADVNVGIAERANLASVERADLFLSIHFNGFNKQTSGVETYIRARTNGNVNFEEDEA
EEECCCCCCCHHCCCCCCCEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCE
FAWRIQGAVLGALRTYLPGTRDRGVKEDTASGGGVLGVLDDRALGNSSQEYPCRACLVEI
EEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEE
EFIDVPRVDRLLNTDAQARQVRGAIAEAIAAAMVEDLQAAR
EEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA