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The map label for this gene is cobM [H]

Identifier: 37520946

GI number: 37520946

Start: 1476124

End: 1476885

Strand: Reverse

Name: cobM [H]

Synonym: gvip197

Alternate gene names: 37520946

Gene position: 1476885-1476124 (Counterclockwise)

Preceding gene: 37520947

Following gene: 37520932

Centisome position: 31.7

GC content: 66.27

Gene sequence:

>762_bases
ATGACGACTTTGCATCCTGTTCAAATTATTGGTGCCGGTCCGGGCGATCCGGATCTCATTACTGTGCGCGGCCAGCGGTT
GCTCGCCCTCGCGGACACCGTCTTCTACGCCGGATCGCTGGTGCCGGAGGGATTGCTCGATTGGTGCCGGCCCGAGGCCG
AACGCATCGACACCCGCTCGCTCACCCTCGAAGCGATTTTGCCGGATCTGCTCGCGCGGGTGCAGACCGGTCGGCGGGTG
GTGCGGCTGCACTCGGGCGATCCGTGCCTCTACGGGGCGGTGCACGAGCAGATGGTGGCCCTGGTCGAAGCAGAGATCGA
ATTTGAAATCGTGCCGGGGATCAGTGCCTTTCAGGCCGCTGCCGCCACTCTCAAAGTCGAACTGACCGTGCCGGGGCTGG
TGCAGACGATTATCCTGACGCGGGCGAGCGGCCGTACCGGCATTCCCGCCGCCGAAGAACTGGCGGGCCTGGCGGCCCAC
CGCGCCTCGCTGTGCCTGTATCTGAGCGCCCACCACGCCACCGAGTGTGAGGAGCGTCTGCTGGCCCACTATCCACCCGA
AACACCGGTGGCCGTCTGCTACCGCCTGGGTTGGCCCGACGAGCGCATCGTGCGCACCCGCCTGGGTGAACTTGCCCGCA
CGACGCGCGAGGAGAAGCTCACCCGCACGACCCTGTACGTGATCAGCCCTGCCCTGGAAGCCGCCCCGTCCCGCTCCCGG
CTGTACCACCCTGAGCACAACCATCTGTTTCGGGCGAAATGA

Upstream 100 bases:

>100_bases
CAGCCGATCTGCAGGCGGTGCTCGGCGCGGCCGAGGAGGAAGTGGCCATCGCCAATTTCCGGGCCTGGGTAAAGCGGCAA
ATCGAAGGACAGCAACCTAC

Downstream 100 bases:

>100_bases
GGTGTCTCCTGACACCTTACCCAAACATGGCCACACTAACGGAGCCGATATGACAGGTTCAGCACGCCTTTGAGCAATTC
TCTCGTGAGGCCGATTATCC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTTLHPVQIIGAGPGDPDLITVRGQRLLALADTVFYAGSLVPEGLLDWCRPEAERIDTRSLTLEAILPDLLARVQTGRRV
VRLHSGDPCLYGAVHEQMVALVEAEIEFEIVPGISAFQAAAATLKVELTVPGLVQTIILTRASGRTGIPAAEELAGLAAH
RASLCLYLSAHHATECEERLLAHYPPETPVAVCYRLGWPDERIVRTRLGELARTTREEKLTRTTLYVISPALEAAPSRSR
LYHPEHNHLFRAK

Sequences:

>Translated_253_residues
MTTLHPVQIIGAGPGDPDLITVRGQRLLALADTVFYAGSLVPEGLLDWCRPEAERIDTRSLTLEAILPDLLARVQTGRRV
VRLHSGDPCLYGAVHEQMVALVEAEIEFEIVPGISAFQAAAATLKVELTVPGLVQTIILTRASGRTGIPAAEELAGLAAH
RASLCLYLSAHHATECEERLLAHYPPETPVAVCYRLGWPDERIVRTRLGELARTTREEKLTRTTLYVISPALEAAPSRSR
LYHPEHNHLFRAK
>Mature_252_residues
TTLHPVQIIGAGPGDPDLITVRGQRLLALADTVFYAGSLVPEGLLDWCRPEAERIDTRSLTLEAILPDLLARVQTGRRVV
RLHSGDPCLYGAVHEQMVALVEAEIEFEIVPGISAFQAAAATLKVELTVPGLVQTIILTRASGRTGIPAAEELAGLAAHR
ASLCLYLSAHHATECEERLLAHYPPETPVAVCYRLGWPDERIVRTRLGELARTTREEKLTRTTLYVISPALEAAPSRSRL
YHPEHNHLFRAK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=224, Percent_Identity=32.1428571428571, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322922, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27768; Mature: 27637

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLHPVQIIGAGPGDPDLITVRGQRLLALADTVFYAGSLVPEGLLDWCRPEAERIDTRS
CCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCHHH
LTLEAILPDLLARVQTGRRVVRLHSGDPCLYGAVHEQMVALVEAEIEFEIVPGISAFQAA
HHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEEEEECCCHHHHHH
AATLKVELTVPGLVQTIILTRASGRTGIPAAEELAGLAAHRASLCLYLSAHHATECEERL
HEEEEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
LAHYPPETPVAVCYRLGWPDERIVRTRLGELARTTREEKLTRTTLYVISPALEAAPSRSR
HHCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCCC
LYHPEHNHLFRAK
CCCCCCCCEECCC
>Mature Secondary Structure 
TTLHPVQIIGAGPGDPDLITVRGQRLLALADTVFYAGSLVPEGLLDWCRPEAERIDTRS
CCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCHHH
LTLEAILPDLLARVQTGRRVVRLHSGDPCLYGAVHEQMVALVEAEIEFEIVPGISAFQAA
HHHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEEEEECCCHHHHHH
AATLKVELTVPGLVQTIILTRASGRTGIPAAEELAGLAAHRASLCLYLSAHHATECEERL
HEEEEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
LAHYPPETPVAVCYRLGWPDERIVRTRLGELARTTREEKLTRTTLYVISPALEAAPSRSR
HHCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCCC
LYHPEHNHLFRAK
CCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]