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The map label for this gene is pmbA [H]

Identifier: 37520930

GI number: 37520930

Start: 1454324

End: 1455610

Strand: Direct

Name: pmbA [H]

Synonym: glr1361

Alternate gene names: 37520930

Gene position: 1454324-1455610 (Clockwise)

Preceding gene: 37520928

Following gene: 37520933

Centisome position: 31.22

GC content: 66.05

Gene sequence:

>1287_bases
ATGGATCCGCAGCGACTGCTCGAACTGGCTAGAAAAAACGGCGCCGAGGCCGCCGAGGTCTATCTCTCGCAGGGGTTGGG
CCGCTCCGCCAATTTCGAGGCCAACCGCCTCAAGCAAGTGGAGACGGGCGATGAGGAAGGGGTGGCCCTACGCGTCTGGC
GCGACGGCCGGCCGGGTCTGGCGGTGGCCCACGGTCCGGTCGACGACGCATTGCTGGTGGAAAAAGCAATCGCCCTCGCC
CAATTCGGTCAGCCGGAGGAACCGGAGCTGGCCGGCGAACCCTTGCTCGTTGAAAGCCGTCCCCATACCCCAACCCCCAT
CGAACGACTGATCGCCTGGGGTGAACAGACGATTTCCCTGGTGCGCGAGCGCTACGCGGAGGTGCTCTGCGGCTGCACCT
ACAGCGAAGAGGTGGGCACCGTCCGCCTGATCAATTCCCGCGGCCTCGATGTGAGCTATGCCAGCCATCACCGCAGCGGC
TACCTGGGGGCTGAGTGGGTGCGCGGCGAAGATTTTTTGCAGGTGTACGACGGTGAGACTGTGCTTGGGGATCCTTCCCC
GGAGGCGGTCGCCGCCCCGGTGCTCGAACGGCTGGGCTGGTCGCAGGAGAACTGTCCTACCCGAGTTGGGACTGTGCCGG
TCCTGTTCACGGCCAAGGCCACCACGGTGCTGTTGGGCACCGTGGGCGCCGCTCTGAACGGCCGTCAGGTACTGCAGGAG
GCTTCACCCTGGAGCCAGAAGCGGGGCGAGATGGTCCTCTCGCCACTCCTCTCCTTGAGCCAGGATCCCCAGTTGGCTCC
CTACATCACGCCTTTTGACGACGAGGGCACCCCGGCTATTGCCCTCGAATTCGTGCGGGCGGGTCGGCTTGAGACGTTTT
ATGCCGATCGGCGCATCGCCCGTCGCCTCGGGCTGCGTCCGGGAGGCAACGGCCTGCGATCCGACCTCGGCAGCTATCCG
CAGCCGGGGCTGTTCAACCTGGTGGTGGCCGCCGGGGAGCATGCGTTTGCCGAACTGCTCAGCCGGATGGACGACGGCAT
CGTGGTCGACCAGGTACTCGGAGGCGGCGCCGGTCTGTCGGGGGAATTTTCCGTCAACATCGATCTGGGTTTTCGGGTCA
AAGGAGGCGAGATTGTCGGGCGCATCAAGGACACGATGGTGGCCGGCAACGCCTACGATGCCCTGGGCCGGCTCGCCGCC
CTCGGCAGCGAGCGGCTCTGGGAGGGCAACGTGTTCACTCCGGCACTGCTGGTCGAATCGCTCGCCGTCACCGGCCGGGC
CGATTAG

Upstream 100 bases:

>100_bases
CGGGATAGTTCACGTTCAGGTAGGGCAAACGGCCGATCGGCAGTCTAACGCACTCTGGGTGCACAATTGATCAGTCCCCC
GACCCAGGAATCCCCCAGCG

Downstream 100 bases:

>100_bases
CCGTTTGCCGCAAGCGCAAACACATCGGCGAGCACGACGTCCCCGGACGGCTTGCGTTTTTGATCAGGAGTGTCGTAAAC
CACCAACAGTTCACCGTCTT

Product: putative modulator of DNA gyrase PmbA-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDPQRLLELARKNGAEAAEVYLSQGLGRSANFEANRLKQVETGDEEGVALRVWRDGRPGLAVAHGPVDDALLVEKAIALA
QFGQPEEPELAGEPLLVESRPHTPTPIERLIAWGEQTISLVRERYAEVLCGCTYSEEVGTVRLINSRGLDVSYASHHRSG
YLGAEWVRGEDFLQVYDGETVLGDPSPEAVAAPVLERLGWSQENCPTRVGTVPVLFTAKATTVLLGTVGAALNGRQVLQE
ASPWSQKRGEMVLSPLLSLSQDPQLAPYITPFDDEGTPAIALEFVRAGRLETFYADRRIARRLGLRPGGNGLRSDLGSYP
QPGLFNLVVAAGEHAFAELLSRMDDGIVVDQVLGGGAGLSGEFSVNIDLGFRVKGGEIVGRIKDTMVAGNAYDALGRLAA
LGSERLWEGNVFTPALLVESLAVTGRAD

Sequences:

>Translated_428_residues
MDPQRLLELARKNGAEAAEVYLSQGLGRSANFEANRLKQVETGDEEGVALRVWRDGRPGLAVAHGPVDDALLVEKAIALA
QFGQPEEPELAGEPLLVESRPHTPTPIERLIAWGEQTISLVRERYAEVLCGCTYSEEVGTVRLINSRGLDVSYASHHRSG
YLGAEWVRGEDFLQVYDGETVLGDPSPEAVAAPVLERLGWSQENCPTRVGTVPVLFTAKATTVLLGTVGAALNGRQVLQE
ASPWSQKRGEMVLSPLLSLSQDPQLAPYITPFDDEGTPAIALEFVRAGRLETFYADRRIARRLGLRPGGNGLRSDLGSYP
QPGLFNLVVAAGEHAFAELLSRMDDGIVVDQVLGGGAGLSGEFSVNIDLGFRVKGGEIVGRIKDTMVAGNAYDALGRLAA
LGSERLWEGNVFTPALLVESLAVTGRAD
>Mature_428_residues
MDPQRLLELARKNGAEAAEVYLSQGLGRSANFEANRLKQVETGDEEGVALRVWRDGRPGLAVAHGPVDDALLVEKAIALA
QFGQPEEPELAGEPLLVESRPHTPTPIERLIAWGEQTISLVRERYAEVLCGCTYSEEVGTVRLINSRGLDVSYASHHRSG
YLGAEWVRGEDFLQVYDGETVLGDPSPEAVAAPVLERLGWSQENCPTRVGTVPVLFTAKATTVLLGTVGAALNGRQVLQE
ASPWSQKRGEMVLSPLLSLSQDPQLAPYITPFDDEGTPAIALEFVRAGRLETFYADRRIARRLGLRPGGNGLRSDLGSYP
QPGLFNLVVAAGEHAFAELLSRMDDGIVVDQVLGGGAGLSGEFSVNIDLGFRVKGGEIVGRIKDTMVAGNAYDALGRLAA
LGSERLWEGNVFTPALLVESLAVTGRAD

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=440, Percent_Identity=24.3181818181818, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789640, Length=276, Percent_Identity=26.8115942028986, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 45960; Mature: 45960

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPQRLLELARKNGAEAAEVYLSQGLGRSANFEANRLKQVETGDEEGVALRVWRDGRPGL
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCE
AVAHGPVDDALLVEKAIALAQFGQPEEPELAGEPLLVESRPHTPTPIERLIAWGEQTISL
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
VRERYAEVLCGCTYSEEVGTVRLINSRGLDVSYASHHRSGYLGAEWVRGEDFLQVYDGET
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCEEECCCCEEEEECCCE
VLGDPSPEAVAAPVLERLGWSQENCPTRVGTVPVLFTAKATTVLLGTVGAALNGRQVLQE
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHEEHHHHHHHHCCHHHHHHH
ASPWSQKRGEMVLSPLLSLSQDPQLAPYITPFDDEGTPAIALEFVRAGRLETFYADRRIA
CCCCHHHCCHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
RRLGLRPGGNGLRSDLGSYPQPGLFNLVVAAGEHAFAELLSRMDDGIVVDQVLGGGAGLS
HHHCCCCCCCHHHHHHCCCCCCCHHEEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCC
GEFSVNIDLGFRVKGGEIVGRIKDTMVAGNAYDALGRLAALGSERLWEGNVFTPALLVES
CEEEEEEEEEEEEECCHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LAVTGRAD
HHHCCCCC
>Mature Secondary Structure
MDPQRLLELARKNGAEAAEVYLSQGLGRSANFEANRLKQVETGDEEGVALRVWRDGRPGL
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCE
AVAHGPVDDALLVEKAIALAQFGQPEEPELAGEPLLVESRPHTPTPIERLIAWGEQTISL
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
VRERYAEVLCGCTYSEEVGTVRLINSRGLDVSYASHHRSGYLGAEWVRGEDFLQVYDGET
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCEEECCCCEEEEECCCE
VLGDPSPEAVAAPVLERLGWSQENCPTRVGTVPVLFTAKATTVLLGTVGAALNGRQVLQE
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHEEHHHHHHHHCCHHHHHHH
ASPWSQKRGEMVLSPLLSLSQDPQLAPYITPFDDEGTPAIALEFVRAGRLETFYADRRIA
CCCCHHHCCHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
RRLGLRPGGNGLRSDLGSYPQPGLFNLVVAAGEHAFAELLSRMDDGIVVDQVLGGGAGLS
HHHCCCCCCCHHHHHHCCCCCCCHHEEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCC
GEFSVNIDLGFRVKGGEIVGRIKDTMVAGNAYDALGRLAALGSERLWEGNVFTPALLVES
CEEEEEEEEEEEEECCHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LAVTGRAD
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]