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The map label for this gene is potD-A [H]

Identifier: 37520928

GI number: 37520928

Start: 1452744

End: 1453829

Strand: Direct

Name: potD-A [H]

Synonym: glr1359

Alternate gene names: 37520928

Gene position: 1452744-1453829 (Clockwise)

Preceding gene: 37520926

Following gene: 37520930

Centisome position: 31.18

GC content: 58.84

Gene sequence:

>1086_bases
ATGCAACGGACGAGCCTGTCTGTGTTTTCTCTGTTTGTCCTTGCCCTGGCGACACTGTCGCTGGTGGCCTGTGGTGCACC
GGCAGGTTCCGGGGACAGTTCAAAGGAACTGAATCTTTACGCCTGGTCGGAATACATTCCGCAAAAGATGCTCGACCAGT
TCACCGAGAAGACAGGGATCCGGGTCAACTACGACACGTTTTCCTCCAACGAAGAGCTGCTCGCCAAATTGCAGGCCGGC
AGTTCCGGCTATGACGTAATCATCGCCAGCGACTACACCGTCGATATCCTCAAAAAGCAAAACCGGCTCGAACCGCTTGA
ATTGGCGAAACTACCCAACTTCGCCAATATCGAAGCAACTTTGAAAAACCCGCCCTACGATCCGGATAATCGCTACACCG
TGCCCTACCAGTGGGGCACCGTCGGCCTGGCGGTCGATACGGCCAAGGTGAAACGGCCGATTAGCCGTTGGGAGGATCTG
TGGGATCCCGCCTTCAAGGGACGGGTGGTACTGCTCGACGACGAATTTGAAGTGCTGGGTATGGCGCTGCAAACTCTAGG
CTTCGACAAAAACACCAAAGACCCTGCCCAACTGGCAAAAGCCCGAGCCAAACTCGCCCAACTGCGCCCGAATATCAAAC
TCTTCGACAGCGACAGCCCGAAGACGGCCCTGCTATCCGGCGAAGTCTGGCTTGGGCAGGTCTGGAACGGCGAAGCGGCC
CTCGCCCAGCGCGAGAACCCGGCCATCCGCTACCTGTGCCCCGCCGAAGGTTGCGGCATCTGGCACGACAACTGGGCCGT
CCCCAAAGGCGCTCCGCACAAAGACGCTGCCCTCGCCTTTATCGACTACACGATGAGCCCGGAGGCGGGCATTATGATCA
CCCAGGCCTTTCCATACTCCAACCCCAACCGGGCGGCCCTGGAACTGCTCAAAAAAGCAGATCCAAAACTCTACTCCACC
TACATGGCCTCCCCCGCCACCAACCCCGGCCCCGCTTTTCTCAAAGCGGCCCAGCCGGTGGAGGACAAAGCCGAGAATAC
GCCGCTGTGGGATCGCACCTGGACCGAGGTCAAAAGCGGCACCTGA

Upstream 100 bases:

>100_bases
CGTTTCAGCAGAGCCACCCTGGCTGGTTCTCTGTTGAATCCGTGGGCAAGAAGGCGAATTTGCCCTATTCTCGGGTCAAC
CATACTCGCCTTTCCAAGAC

Downstream 100 bases:

>100_bases
AACAGGGCTCAGGTGGCCCGACCGGTAAGACGCCTCAGCCAGCGCTTGAAGCGGCCCAGCAACGACGGCGGGTCACTCGC
TTCGAGGGCGTGGCGCAGAT

Product: periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MQRTSLSVFSLFVLALATLSLVACGAPAGSGDSSKELNLYAWSEYIPQKMLDQFTEKTGIRVNYDTFSSNEELLAKLQAG
SSGYDVIIASDYTVDILKKQNRLEPLELAKLPNFANIEATLKNPPYDPDNRYTVPYQWGTVGLAVDTAKVKRPISRWEDL
WDPAFKGRVVLLDDEFEVLGMALQTLGFDKNTKDPAQLAKARAKLAQLRPNIKLFDSDSPKTALLSGEVWLGQVWNGEAA
LAQRENPAIRYLCPAEGCGIWHDNWAVPKGAPHKDAALAFIDYTMSPEAGIMITQAFPYSNPNRAALELLKKADPKLYST
YMASPATNPGPAFLKAAQPVEDKAENTPLWDRTWTEVKSGT

Sequences:

>Translated_361_residues
MQRTSLSVFSLFVLALATLSLVACGAPAGSGDSSKELNLYAWSEYIPQKMLDQFTEKTGIRVNYDTFSSNEELLAKLQAG
SSGYDVIIASDYTVDILKKQNRLEPLELAKLPNFANIEATLKNPPYDPDNRYTVPYQWGTVGLAVDTAKVKRPISRWEDL
WDPAFKGRVVLLDDEFEVLGMALQTLGFDKNTKDPAQLAKARAKLAQLRPNIKLFDSDSPKTALLSGEVWLGQVWNGEAA
LAQRENPAIRYLCPAEGCGIWHDNWAVPKGAPHKDAALAFIDYTMSPEAGIMITQAFPYSNPNRAALELLKKADPKLYST
YMASPATNPGPAFLKAAQPVEDKAENTPLWDRTWTEVKSGT
>Mature_361_residues
MQRTSLSVFSLFVLALATLSLVACGAPAGSGDSSKELNLYAWSEYIPQKMLDQFTEKTGIRVNYDTFSSNEELLAKLQAG
SSGYDVIIASDYTVDILKKQNRLEPLELAKLPNFANIEATLKNPPYDPDNRYTVPYQWGTVGLAVDTAKVKRPISRWEDL
WDPAFKGRVVLLDDEFEVLGMALQTLGFDKNTKDPAQLAKARAKLAQLRPNIKLFDSDSPKTALLSGEVWLGQVWNGEAA
LAQRENPAIRYLCPAEGCGIWHDNWAVPKGAPHKDAALAFIDYTMSPEAGIMITQAFPYSNPNRAALELLKKADPKLYST
YMASPATNPGPAFLKAAQPVEDKAENTPLWDRTWTEVKSGT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=285, Percent_Identity=38.5964912280702, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1787078, Length=371, Percent_Identity=31.8059299191375, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787711, Length=308, Percent_Identity=24.3506493506494, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39865; Mature: 39865

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTSLSVFSLFVLALATLSLVACGAPAGSGDSSKELNLYAWSEYIPQKMLDQFTEKTGI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
RVNYDTFSSNEELLAKLQAGSSGYDVIIASDYTVDILKKQNRLEPLELAKLPNFANIEAT
EEEECCCCCCHHHHHHHHCCCCCCEEEEECCCEEEEHHHCCCCCCHHHHHCCCCCCEEEE
LKNPPYDPDNRYTVPYQWGTVGLAVDTAKVKRPISRWEDLWDPAFKGRVVLLDDEFEVLG
ECCCCCCCCCCEEEEEECCEEEEEECHHHHHHHHHHHHHHCCHHHCCEEEEECCCHHHHH
MALQTLGFDKNTKDPAQLAKARAKLAQLRPNIKLFDSDSPKTALLSGEVWLGQVWNGEAA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEEECCCCH
LAQRENPAIRYLCPAEGCGIWHDNWAVPKGAPHKDAALAFIDYTMSPEAGIMITQAFPYS
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCC
NPNRAALELLKKADPKLYSTYMASPATNPGPAFLKAAQPVEDKAENTPLWDRTWTEVKSG
CCCHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHCCCCCCCCCHHHHHCCC
T
C
>Mature Secondary Structure
MQRTSLSVFSLFVLALATLSLVACGAPAGSGDSSKELNLYAWSEYIPQKMLDQFTEKTGI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
RVNYDTFSSNEELLAKLQAGSSGYDVIIASDYTVDILKKQNRLEPLELAKLPNFANIEAT
EEEECCCCCCHHHHHHHHCCCCCCEEEEECCCEEEEHHHCCCCCCHHHHHCCCCCCEEEE
LKNPPYDPDNRYTVPYQWGTVGLAVDTAKVKRPISRWEDLWDPAFKGRVVLLDDEFEVLG
ECCCCCCCCCCEEEEEECCEEEEEECHHHHHHHHHHHHHHCCHHHCCEEEEECCCHHHHH
MALQTLGFDKNTKDPAQLAKARAKLAQLRPNIKLFDSDSPKTALLSGEVWLGQVWNGEAA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEEECCCCH
LAQRENPAIRYLCPAEGCGIWHDNWAVPKGAPHKDAALAFIDYTMSPEAGIMITQAFPYS
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCC
NPNRAALELLKKADPKLYSTYMASPATNPGPAFLKAAQPVEDKAENTPLWDRTWTEVKSG
CCCHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHCCCCCCCCCHHHHHCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]