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The map label for this gene is hemD [H]

Identifier: 37520803

GI number: 37520803

Start: 1310706

End: 1312232

Strand: Direct

Name: hemD [H]

Synonym: gvip169

Alternate gene names: 37520803

Gene position: 1310706-1312232 (Clockwise)

Preceding gene: 37520802

Following gene: 37520804

Centisome position: 28.13

GC content: 67.71

Gene sequence:

>1527_bases
ATGCCGGGTACGGTCTATCTGGTGGGGGCGGGACCGGCCGGGGCCGAGTATTTGACCGTCCGCGGCCGGGAGCTGTTGGA
GCAGGCCGAGGTCGTTCTCTACGACGATCTGGCAGACCCGCACTTGCTGAAACTCGCTAGTGCGGCCCAACTCATCGATG
TCGGCAAGCGGGCCGGCCGGCCGGGAGCCGACCAGGGCGAGATCGGCCGCCTGCTGGTAACCCACTGTCAGGCGGGCCGT
AGGGTGGTGCGGCTAAAGGCGGGCGACCCGCTTGTCTTTGGCCGCGCTGCGGCGGAACTAGGAGCGCTGGTGGCGGCCGG
GTGCAACTTTGAGATCGTGCCGGGGGTGAGTTCCGCCCTTGCCGCCTGCGCGTTTGCCGGCATCCCGCTCACCGACCGTG
CCCTCGGCCGCCACTTTTGCGTGATGAGTGGCCACGATATCGGCACCCTACCCTGGCAGCATCTGGCCCACATCGACACG
CTCGTCATCTTGATGGGCACCCGCCACCTCGAGGCGATCGCCGAGCAGCTGATGGACTACGGCCGTCCGGCGGCGATGCC
GGTGGCGGTGATCTTCTGGGCAGGGCGGTCCGAGCAGCGCACGGTGGTGGGTACCCTCGAAGACATCGCAAGCCGGGTTG
CGCCCTCCGGCGAGCCGGCGGTGATCGTCGTGGGACCGGTGGTCCGCCGCCGCGAGTGCTTCGACTGGTTTGACCGCCGA
GCGCTGTTTGGAAAACACATCCTCGTCACCCGCGCGGCCGATCAAGCCGGGGGATTCAGTGCCCAACTGGAGGCACTGGG
TGCCTCGGTCCTGGAGATGCCCGCCCTGGTAGTCACTGCGCCCACCAGCTGGGAACCGCTCGATCGGGCCATTGCCCAGC
TGGCCGGCTACCGCTGGCTGTTGCTGACCTCCGCCAACGGCGTGGAGGCGTTCTTTGCGCGTCTGACCCACCACAGACGC
GACCTGCGCGCCCTGGGCGGGGTGCGCGTCGCCGCCGTCGGACCCAAGACTGCCCAGGCCGCCCGCACCCATGGACTGAT
CTGCGACTTCGTACCGGGTCAGTACGTCTCCGACGGACTGCTTACCGATTTTCCGGACATCGCCGGGATCTATGGGGAGC
GGGTGCTCTTTGTGCGGGTCGAATCCGGAGGCCGCGAGACGATCACCGAGCAGTTTCGCCGCTGGGGCGCTCTTGTCGAT
GAGGTCGCAGGCTACGCTACCGGCTGCCCGGATAGCGCCGAGCCGGGCTGCGTCGCCGCTCTACGCGCAGGCCGGATCGA
CTGTGCCACCTTCGCCAGCGCCAAAACCGTGCGCCATTTTGCCCGCCTCCTCCAAGGCGAGGAGCTGGAATCGCTTCTGA
GAAAAGTGCGTATTGCTTCGATCGGGCCGCAGACCAGTGCTGCCTGCCGCGAGACACTGGGGCGGGTGGACGTCGAGGCC
GAAGTCTTTACCCTCGATGGACTCGCCCAGGCGATTGTCGCCGATCTCGGCACCGGCAGGGCGACCGGGCCGACAGACGA
ACAATGA

Upstream 100 bases:

>100_bases
ATCTGCGCACTGAGGAGGAAACCTCCGATGTTACCGGTGTGATGAACGGCAAGATCGAGCCGTTTATCGAGCGCTATTTG
CGCCGGCAGCACAAGCTCTG

Downstream 100 bases:

>100_bases
TATCCTGGGAAAGTGTGCAAACAAACTTTACCGTGGAGCATATGACCGCCCAGAGCCCTGCCACCCAGGCCACGCCTATC
TCCTGGGTCTTCGTCGGTTT

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MPGTVYLVGAGPAGAEYLTVRGRELLEQAEVVLYDDLADPHLLKLASAAQLIDVGKRAGRPGADQGEIGRLLVTHCQAGR
RVVRLKAGDPLVFGRAAAELGALVAAGCNFEIVPGVSSALAACAFAGIPLTDRALGRHFCVMSGHDIGTLPWQHLAHIDT
LVILMGTRHLEAIAEQLMDYGRPAAMPVAVIFWAGRSEQRTVVGTLEDIASRVAPSGEPAVIVVGPVVRRRECFDWFDRR
ALFGKHILVTRAADQAGGFSAQLEALGASVLEMPALVVTAPTSWEPLDRAIAQLAGYRWLLLTSANGVEAFFARLTHHRR
DLRALGGVRVAAVGPKTAQAARTHGLICDFVPGQYVSDGLLTDFPDIAGIYGERVLFVRVESGGRETITEQFRRWGALVD
EVAGYATGCPDSAEPGCVAALRAGRIDCATFASAKTVRHFARLLQGEELESLLRKVRIASIGPQTSAACRETLGRVDVEA
EVFTLDGLAQAIVADLGTGRATGPTDEQ

Sequences:

>Translated_508_residues
MPGTVYLVGAGPAGAEYLTVRGRELLEQAEVVLYDDLADPHLLKLASAAQLIDVGKRAGRPGADQGEIGRLLVTHCQAGR
RVVRLKAGDPLVFGRAAAELGALVAAGCNFEIVPGVSSALAACAFAGIPLTDRALGRHFCVMSGHDIGTLPWQHLAHIDT
LVILMGTRHLEAIAEQLMDYGRPAAMPVAVIFWAGRSEQRTVVGTLEDIASRVAPSGEPAVIVVGPVVRRRECFDWFDRR
ALFGKHILVTRAADQAGGFSAQLEALGASVLEMPALVVTAPTSWEPLDRAIAQLAGYRWLLLTSANGVEAFFARLTHHRR
DLRALGGVRVAAVGPKTAQAARTHGLICDFVPGQYVSDGLLTDFPDIAGIYGERVLFVRVESGGRETITEQFRRWGALVD
EVAGYATGCPDSAEPGCVAALRAGRIDCATFASAKTVRHFARLLQGEELESLLRKVRIASIGPQTSAACRETLGRVDVEA
EVFTLDGLAQAIVADLGTGRATGPTDEQ
>Mature_507_residues
PGTVYLVGAGPAGAEYLTVRGRELLEQAEVVLYDDLADPHLLKLASAAQLIDVGKRAGRPGADQGEIGRLLVTHCQAGRR
VVRLKAGDPLVFGRAAAELGALVAAGCNFEIVPGVSSALAACAFAGIPLTDRALGRHFCVMSGHDIGTLPWQHLAHIDTL
VILMGTRHLEAIAEQLMDYGRPAAMPVAVIFWAGRSEQRTVVGTLEDIASRVAPSGEPAVIVVGPVVRRRECFDWFDRRA
LFGKHILVTRAADQAGGFSAQLEALGASVLEMPALVVTAPTSWEPLDRAIAQLAGYRWLLLTSANGVEAFFARLTHHRRD
LRALGGVRVAAVGPKTAQAARTHGLICDFVPGQYVSDGLLTDFPDIAGIYGERVLFVRVESGGRETITEQFRRWGALVDE
VAGYATGCPDSAEPGCVAALRAGRIDCATFASAKTVRHFARLLQGEELESLLRKVRIASIGPQTSAACRETLGRVDVEAE
VFTLDGLAQAIVADLGTGRATGPTDEQ

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=39.7489539748954, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=28.8702928870293, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54015; Mature: 53883

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTVYLVGAGPAGAEYLTVRGRELLEQAEVVLYDDLADPHLLKLASAAQLIDVGKRAGR
CCCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
PGADQGEIGRLLVTHCQAGRRVVRLKAGDPLVFGRAAAELGALVAAGCNFEIVPGVSSAL
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEECHHHHHHHHHHHCCCCEEECCCHHHHH
AACAFAGIPLTDRALGRHFCVMSGHDIGTLPWQHLAHIDTLVILMGTRHLEAIAEQLMDY
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GRPAAMPVAVIFWAGRSEQRTVVGTLEDIASRVAPSGEPAVIVVGPVVRRRECFDWFDRR
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
ALFGKHILVTRAADQAGGFSAQLEALGASVLEMPALVVTAPTSWEPLDRAIAQLAGYRWL
HHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCEEE
LLTSANGVEAFFARLTHHRRDLRALGGVRVAAVGPKTAQAARTHGLICDFVPGQYVSDGL
EEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCEEECCCCCHHCCCC
LTDFPDIAGIYGERVLFVRVESGGRETITEQFRRWGALVDEVAGYATGCPDSAEPGCVAA
CCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LRAGRIDCATFASAKTVRHFARLLQGEELESLLRKVRIASIGPQTSAACRETLGRVDVEA
HHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCE
EVFTLDGLAQAIVADLGTGRATGPTDEQ
EEEEHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PGTVYLVGAGPAGAEYLTVRGRELLEQAEVVLYDDLADPHLLKLASAAQLIDVGKRAGR
CCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
PGADQGEIGRLLVTHCQAGRRVVRLKAGDPLVFGRAAAELGALVAAGCNFEIVPGVSSAL
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEECHHHHHHHHHHHCCCCEEECCCHHHHH
AACAFAGIPLTDRALGRHFCVMSGHDIGTLPWQHLAHIDTLVILMGTRHLEAIAEQLMDY
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GRPAAMPVAVIFWAGRSEQRTVVGTLEDIASRVAPSGEPAVIVVGPVVRRRECFDWFDRR
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
ALFGKHILVTRAADQAGGFSAQLEALGASVLEMPALVVTAPTSWEPLDRAIAQLAGYRWL
HHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCEEE
LLTSANGVEAFFARLTHHRRDLRALGGVRVAAVGPKTAQAARTHGLICDFVPGQYVSDGL
EEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCEEECCCCCHHCCCC
LTDFPDIAGIYGERVLFVRVESGGRETITEQFRRWGALVDEVAGYATGCPDSAEPGCVAA
CCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
LRAGRIDCATFASAKTVRHFARLLQGEELESLLRKVRIASIGPQTSAACRETLGRVDVEA
HHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCE
EVFTLDGLAQAIVADLGTGRATGPTDEQ
EEEEHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]