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The map label for this gene is prfB [H]

Identifier: 37520802

GI number: 37520802

Start: 1309642

End: 1310706

Strand: Direct

Name: prfB [H]

Synonym: glr1233

Alternate gene names: 37520802

Gene position: 1309642-1310706 (Clockwise)

Preceding gene: 37520801

Following gene: 37520803

Centisome position: 28.11

GC content: 61.97

Gene sequence:

>1065_bases
GTGCGCGCCTGGGTAAAACCCAGGACTATCTTTGACGTCGCCCAACGCCAAATCCGCATTGCCGCCCTGGAGCAGCAGGC
CAGCGCCCCTGATTTTTGGGACGATCAAGCCGCGGCCCAAAAGGCCCTCCAGAACCTCAACAACTTGAAGCAACCCCTGG
AGCAGTTCGGCCGCTGGCGCAACCTGCTCTCCGACGCCGAGGTGGTACTCGAACTGGTCCAGGAGACCGGTGACGAGTCG
CTGCTGCCGGAGGCGGAAGGCAATATCGAAGCGCTCGTGGGCGAACTCGATCGCTGGGAACTGGAGCAGTTGCTGAGCGG
CCCCTACGACGAGCGGGGGGCGATTCTCAGCATCAACGCCGGGGCGGGGGGCACCGACGCCCAGGATTGGGCTGAGATGC
TTTTGCGCATGTACACCCGCTGGGCCGAGCGCCAGGGCTACCAGGCGCACCTGTTGGAGCTGTCGGAAGGTGAAGAAGCG
GGGGTCAAATCGGCGACCCTTGAGATCGACGGTCGCTACGCCTACGGCTATTTGTCGGCCGAAAAAGGCACCCACCGCCT
GGTGCGCATCTCGCCCTTCAACGCCAACGACAAGCGCCAGACCAGCTTCGCAGGGGTTGAGGTGATGCCGGTCCTCGACG
ACTCGGTGCAGGTGGATATCCGCCCCGACGACATCGAAATCGATACGTTCCGCTCCGGCGGGAAGGGCGGCCAGAACGTC
AACAAGGTGGAAACGGGGGTACGCGTCACCCACAAACCCACCGGCATCTCGGTGCGCTGCACCCAGGAGCGCGCCCAGCT
GCAAAACCGCAATAAGGCGATGGAGATGCTTAAAGCCCGCCTGCTGGTGCTCGAAGAAGAAAAGCGCCAGCAACATTTGA
GCGACATTCGCGGCGAAGCGGTGGATGCGGCCTGGGGTAACCAGATCCGCTCCTATGTCTTTCATCCGTACCAGATGGTC
AAAGATCTGCGCACTGAGGAGGAAACCTCCGATGTTACCGGTGTGATGAACGGCAAGATCGAGCCGTTTATCGAGCGCTA
TTTGCGCCGGCAGCACAAGCTCTGA

Upstream 100 bases:

>100_bases
CACCCGCCGGTTCACCAGCAGCGAGCCACCGCAATGGACACCGTCGAGAGCAAAGCCGGGCGCGACGCCGCCCAGATCAA
GCGTCAGGTCGAAGAACTCA

Downstream 100 bases:

>100_bases
TGCCGGGTACGGTCTATCTGGTGGGGGCGGGACCGGCCGGGGCCGAGTATTTGACCGTCCGCGGCCGGGAGCTGTTGGAG
CAGGCCGAGGTCGTTCTCTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRAWVKPRTIFDVAQRQIRIAALEQQASAPDFWDDQAAAQKALQNLNNLKQPLEQFGRWRNLLSDAEVVLELVQETGDES
LLPEAEGNIEALVGELDRWELEQLLSGPYDERGAILSINAGAGGTDAQDWAEMLLRMYTRWAERQGYQAHLLELSEGEEA
GVKSATLEIDGRYAYGYLSAEKGTHRLVRISPFNANDKRQTSFAGVEVMPVLDDSVQVDIRPDDIEIDTFRSGGKGGQNV
NKVETGVRVTHKPTGISVRCTQERAQLQNRNKAMEMLKARLLVLEEEKRQQHLSDIRGEAVDAAWGNQIRSYVFHPYQMV
KDLRTEEETSDVTGVMNGKIEPFIERYLRRQHKL

Sequences:

>Translated_354_residues
MRAWVKPRTIFDVAQRQIRIAALEQQASAPDFWDDQAAAQKALQNLNNLKQPLEQFGRWRNLLSDAEVVLELVQETGDES
LLPEAEGNIEALVGELDRWELEQLLSGPYDERGAILSINAGAGGTDAQDWAEMLLRMYTRWAERQGYQAHLLELSEGEEA
GVKSATLEIDGRYAYGYLSAEKGTHRLVRISPFNANDKRQTSFAGVEVMPVLDDSVQVDIRPDDIEIDTFRSGGKGGQNV
NKVETGVRVTHKPTGISVRCTQERAQLQNRNKAMEMLKARLLVLEEEKRQQHLSDIRGEAVDAAWGNQIRSYVFHPYQMV
KDLRTEEETSDVTGVMNGKIEPFIERYLRRQHKL
>Mature_354_residues
MRAWVKPRTIFDVAQRQIRIAALEQQASAPDFWDDQAAAQKALQNLNNLKQPLEQFGRWRNLLSDAEVVLELVQETGDES
LLPEAEGNIEALVGELDRWELEQLLSGPYDERGAILSINAGAGGTDAQDWAEMLLRMYTRWAERQGYQAHLLELSEGEEA
GVKSATLEIDGRYAYGYLSAEKGTHRLVRISPFNANDKRQTSFAGVEVMPVLDDSVQVDIRPDDIEIDTFRSGGKGGQNV
NKVETGVRVTHKPTGISVRCTQERAQLQNRNKAMEMLKARLLVLEEEKRQQHLSDIRGEAVDAAWGNQIRSYVFHPYQMV
KDLRTEEETSDVTGVMNGKIEPFIERYLRRQHKL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=249, Percent_Identity=34.136546184739, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI166795303, Length=232, Percent_Identity=33.6206896551724, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI166795305, Length=145, Percent_Identity=33.1034482758621, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=40.6432748538012, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=40, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17542784, Length=240, Percent_Identity=31.6666666666667, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=304, Percent_Identity=30.5921052631579, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921226, Length=237, Percent_Identity=35.0210970464135, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40134; Mature: 40134

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAWVKPRTIFDVAQRQIRIAALEQQASAPDFWDDQAAAQKALQNLNNLKQPLEQFGRWR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLSDAEVVLELVQETGDESLLPEAEGNIEALVGELDRWELEQLLSGPYDERGAILSINA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GAGGTDAQDWAEMLLRMYTRWAERQGYQAHLLELSEGEEAGVKSATLEIDGRYAYGYLSA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCEEEEEEEEC
EKGTHRLVRISPFNANDKRQTSFAGVEVMPVLDDSVQVDIRPDDIEIDTFRSGGKGGQNV
CCCCCEEEEEECCCCCCCCHHHHCCEEEEEECCCCEEEEECCCCEEEECCCCCCCCCCCC
NKVETGVRVTHKPTGISVRCTQERAQLQNRNKAMEMLKARLLVLEEEKRQQHLSDIRGEA
HHHHCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VDAAWGNQIRSYVFHPYQMVKDLRTEEETSDVTGVMNGKIEPFIERYLRRQHKL
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAWVKPRTIFDVAQRQIRIAALEQQASAPDFWDDQAAAQKALQNLNNLKQPLEQFGRWR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NLLSDAEVVLELVQETGDESLLPEAEGNIEALVGELDRWELEQLLSGPYDERGAILSINA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GAGGTDAQDWAEMLLRMYTRWAERQGYQAHLLELSEGEEAGVKSATLEIDGRYAYGYLSA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCEEEEEEEEC
EKGTHRLVRISPFNANDKRQTSFAGVEVMPVLDDSVQVDIRPDDIEIDTFRSGGKGGQNV
CCCCCEEEEEECCCCCCCCHHHHCCEEEEEECCCCEEEEECCCCEEEECCCCCCCCCCCC
NKVETGVRVTHKPTGISVRCTQERAQLQNRNKAMEMLKARLLVLEEEKRQQHLSDIRGEA
HHHHCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VDAAWGNQIRSYVFHPYQMVKDLRTEEETSDVTGVMNGKIEPFIERYLRRQHKL
HHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]