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The map label for this gene is tpiA

Identifier: 37520609

GI number: 37520609

Start: 1114986

End: 1115708

Strand: Reverse

Name: tpiA

Synonym: gvip142

Alternate gene names: 37520609

Gene position: 1115708-1114986 (Counterclockwise)

Preceding gene: 37520616

Following gene: 37520607

Centisome position: 23.95

GC content: 62.24

Gene sequence:

>723_bases
TTGCGACGAAAGGTTCTGGCGGGCAACTGGAAAATGTATAAGACCCGCGGCGAAGCGCGCGCCTTCCTGGAGGCTTTTGT
ACCGCTCATCTCCCCGGGCGCCGAGAACCGGGAGGTCATTCTCTGCGGGCCGTTTACCTGCCTCGACCTATTGAGCGCCC
AGGCCGGACCCTATGCCGTCGGCGCACAGAACGTGCACTGGGCCGATCACGGCGCCTACACCGGCGAAATCGCTCCCCAG
ATGCTCGTCGAACTGGGTGTGCACTACGTGATTGTCGGCCATTCTGAGCGGCGCGAATACTTCAACGAGACCGACAGCAC
CGTCAACCGACGCCTCAACAACGCCCAGGATCACGACCTCGTCCCGATTCTCTGCGTGGGTGAGACCGAGAGCGTGCGCA
AGGACGGCATCACCGAAGCCCACATCCGCTCCCAGCTCGATCGCGACCTCGAACTGGTGGACATGCGCCGCCTGATCATT
GCCTACGAACCCATCTGGGCCATCGGCACCGGCAAAACCTGCGAGGCGAACGAAGCGAACCGGGTGTGCGCCATGATCCG
CAAACACGTCAACTTCGAAGGGGTGCCCATCCTCTACGGCGGCTCGGTCAAACCCGAAAATATCGACGAGTTGATGGCCC
AGAGCGACATCGACGGCGTGCTGGTGGGCGGCGCCAGCCTCGAAGCGAAGAGCTTCGCGCGGATCGTCAACTTCGAAGTG
TGA

Upstream 100 bases:

>100_bases
GCCGTTCCAACCACTGTACCGCGCCGAAGCGGCGGGTGCTTTCCGGGCGGATGGGCTGGTACACTGCTGAGGGTAAATCG
GATATCGGGGTGTCACTGCC

Downstream 100 bases:

>100_bases
CGCCTACGAATTGAGCACGTGAAAGAGCGCAAACCAAAAAACAGTCAGGCCGCTCAGCGCGGCCAGATCCAGGCAGGCGC
GCGAGAAAGTCTTGCCTGAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRRKVLAGNWKMYKTRGEARAFLEAFVPLISPGAENREVILCGPFTCLDLLSAQAGPYAVGAQNVHWADHGAYTGEIAPQ
MLVELGVHYVIVGHSERREYFNETDSTVNRRLNNAQDHDLVPILCVGETESVRKDGITEAHIRSQLDRDLELVDMRRLII
AYEPIWAIGTGKTCEANEANRVCAMIRKHVNFEGVPILYGGSVKPENIDELMAQSDIDGVLVGGASLEAKSFARIVNFEV

Sequences:

>Translated_240_residues
MRRKVLAGNWKMYKTRGEARAFLEAFVPLISPGAENREVILCGPFTCLDLLSAQAGPYAVGAQNVHWADHGAYTGEIAPQ
MLVELGVHYVIVGHSERREYFNETDSTVNRRLNNAQDHDLVPILCVGETESVRKDGITEAHIRSQLDRDLELVDMRRLII
AYEPIWAIGTGKTCEANEANRVCAMIRKHVNFEGVPILYGGSVKPENIDELMAQSDIDGVLVGGASLEAKSFARIVNFEV
>Mature_240_residues
MRRKVLAGNWKMYKTRGEARAFLEAFVPLISPGAENREVILCGPFTCLDLLSAQAGPYAVGAQNVHWADHGAYTGEIAPQ
MLVELGVHYVIVGHSERREYFNETDSTVNRRLNNAQDHDLVPILCVGETESVRKDGITEAHIRSQLDRDLELVDMRRLII
AYEPIWAIGTGKTCEANEANRVCAMIRKHVNFEGVPILYGGSVKPENIDELMAQSDIDGVLVGGASLEAKSFARIVNFEV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=36.1788617886179, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=36.1788617886179, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=38.7096774193548, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17536593, Length=248, Percent_Identity=36.6935483870968, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=36.9918699186992, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=36.9477911646586, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=36.9477911646586, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=36.9477911646586, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_GLOVI (Q7NLT1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923986.1
- ProteinModelPortal:   Q7NLT1
- SMR:   Q7NLT1
- GeneID:   2599076
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1040
- HOGENOM:   HBG708281
- OMA:   AGSGKFF
- ProtClustDB:   PRK00042
- BioCyc:   GVIO251221:GLL1040-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26609; Mature: 26609

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 163-163 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKVLAGNWKMYKTRGEARAFLEAFVPLISPGAENREVILCGPFTCLDLLSAQAGPYAV
CCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEE
GAQNVHWADHGAYTGEIAPQMLVELGVHYVIVGHSERREYFNETDSTVNRRLNNAQDHDL
CCCCCEECCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHHCHHHHHHHHHHCCCCCCCC
VPILCVGETESVRKDGITEAHIRSQLDRDLELVDMRRLIIAYEPIWAIGTGKTCEANEAN
EEEEEECCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEECCEEEECCCCCCCCCCHH
RVCAMIRKHVNFEGVPILYGGSVKPENIDELMAQSDIDGVLVGGASLEAKSFARIVNFEV
HHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRRKVLAGNWKMYKTRGEARAFLEAFVPLISPGAENREVILCGPFTCLDLLSAQAGPYAV
CCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEE
GAQNVHWADHGAYTGEIAPQMLVELGVHYVIVGHSERREYFNETDSTVNRRLNNAQDHDL
CCCCCEECCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHHCHHHHHHHHHHCCCCCCCC
VPILCVGETESVRKDGITEAHIRSQLDRDLELVDMRRLIIAYEPIWAIGTGKTCEANEAN
EEEEEECCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEECCEEEECCCCCCCCCCHH
RVCAMIRKHVNFEGVPILYGGSVKPENIDELMAQSDIDGVLVGGASLEAKSFARIVNFEV
HHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292