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The map label for this gene is dus1 [H]

Identifier: 37520616

GI number: 37520616

Start: 1121072

End: 1122100

Strand: Reverse

Name: dus1 [H]

Synonym: gll1047

Alternate gene names: 37520616

Gene position: 1122100-1121072 (Counterclockwise)

Preceding gene: 37520617

Following gene: 37520609

Centisome position: 24.08

GC content: 65.4

Gene sequence:

>1029_bases
ATGGAACTGTCCGCCACCCACCGGGCACGCCTCGCTTCACCCCTCACCATCGGTTCGCACCGCTTCGCCAGCCGCGTGCT
CCAATCGCCCCTGGCCGGGGTGACCGACAAGGCGTTTCGGCAACTGGTGCGCCGCTATGCGCCGCACTCGCTGTTGTACA
CCGAGATGGTGAGCGCTTCGGGGCTGCACTACAGCCGCTCGCTGCCCCAGATCATGGACATCGCGCCGGGCGAGACGCCC
ATCGGCATCCAGCTGTTCGATTGCCGCCCCGACTTTCTGGCGGAGGCGGCGCGCGTCGCCGAGGCCCAGGGTGCCGATGT
GATCGACATCAACATGGGTTGCCCGGTCAACAAGATCACCAAAAACGGCGGCGGCTCCTCGCTCCTGCGCGATCCGGCCA
CCGCGGCCAAAATCGTCGCCGTAGTCGCGGCGGCGGTAAGCCTGCCGGTCACCGTCAAGACGCGCCTGGGCTGGAGCGAC
GGGGAGATCAATATCCTCGAATTTGCGCGCCTGATGCAAGAAAGCGGCGCCCAGATGCTCACGGTCCACGCCCGCACCCG
CGCCCAGAGTTTCGAAGGCACCGCCCGCTGGGAGTGGATAGCCCGGGTCAAAGCGCAACTGGCCATCCCAGTCATCGCCA
ACGGCGACATCTTCTCAATCGACGCGGCGATTCGCTGTCTGGAGTTGACCGGAGCGGACGGCGTCATGTGCGCGCGCGGC
ACGATGGGGGCTCCGCACTTGGTGGGTCAGATCGATCACTATTTGCGCACCGGCGAGCGACTACCCGATCCGACACCCCG
CGCGCGCCTGGAGATGGCCTGTGTCCATCTCCACTTGCTTTTTGACTATCTCGGAGCGCCGGGGGTACGCCGGGCGCGCA
AGCACATGGGCTGGTACGTGCGCGACTTTCCCGGAGCGGCGGCTTTGCGCGCCCAACTGATGTGCATTGAGACTGTACCC
GAGGGAGAAAGGCTGCTGAACGAGGCCATCGAGCGTCTGGAAGCGAAGCCGGTATTGAGTGCAAATTAG

Upstream 100 bases:

>100_bases
TCGACATCGACTACGAAACTATCGCCGTTCCCGAAGGGGGCAGCATGTTGATGGTCTCCGTCTCCGGCACCGCCGTCAAA
CTCAGCTGAGGGAACCCCCC

Downstream 100 bases:

>100_bases
AGCTCGTGGCAACCGGTGCGAATGCCTAGGATTTAATGGTACAACTTTCAAGCATTACGAGGTATTGCTAGCCAAGTATG
CCGACAAGGTTTCTGTTGTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MELSATHRARLASPLTIGSHRFASRVLQSPLAGVTDKAFRQLVRRYAPHSLLYTEMVSASGLHYSRSLPQIMDIAPGETP
IGIQLFDCRPDFLAEAARVAEAQGADVIDINMGCPVNKITKNGGGSSLLRDPATAAKIVAVVAAAVSLPVTVKTRLGWSD
GEINILEFARLMQESGAQMLTVHARTRAQSFEGTARWEWIARVKAQLAIPVIANGDIFSIDAAIRCLELTGADGVMCARG
TMGAPHLVGQIDHYLRTGERLPDPTPRARLEMACVHLHLLFDYLGAPGVRRARKHMGWYVRDFPGAAALRAQLMCIETVP
EGERLLNEAIERLEAKPVLSAN

Sequences:

>Translated_342_residues
MELSATHRARLASPLTIGSHRFASRVLQSPLAGVTDKAFRQLVRRYAPHSLLYTEMVSASGLHYSRSLPQIMDIAPGETP
IGIQLFDCRPDFLAEAARVAEAQGADVIDINMGCPVNKITKNGGGSSLLRDPATAAKIVAVVAAAVSLPVTVKTRLGWSD
GEINILEFARLMQESGAQMLTVHARTRAQSFEGTARWEWIARVKAQLAIPVIANGDIFSIDAAIRCLELTGADGVMCARG
TMGAPHLVGQIDHYLRTGERLPDPTPRARLEMACVHLHLLFDYLGAPGVRRARKHMGWYVRDFPGAAALRAQLMCIETVP
EGERLLNEAIERLEAKPVLSAN
>Mature_342_residues
MELSATHRARLASPLTIGSHRFASRVLQSPLAGVTDKAFRQLVRRYAPHSLLYTEMVSASGLHYSRSLPQIMDIAPGETP
IGIQLFDCRPDFLAEAARVAEAQGADVIDINMGCPVNKITKNGGGSSLLRDPATAAKIVAVVAAAVSLPVTVKTRLGWSD
GEINILEFARLMQESGAQMLTVHARTRAQSFEGTARWEWIARVKAQLAIPVIANGDIFSIDAAIRCLELTGADGVMCARG
TMGAPHLVGQIDHYLRTGERLPDPTPRARLEMACVHLHLLFDYLGAPGVRRARKHMGWYVRDFPGAAALRAQLMCIETVP
EGERLLNEAIERLEAKPVLSAN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=240, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI239788483, Length=279, Percent_Identity=27.2401433691756, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI239788462, Length=242, Percent_Identity=29.7520661157025, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI31742496, Length=258, Percent_Identity=30.2325581395349, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=37.2670807453416, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788462, Length=323, Percent_Identity=30.6501547987616, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=25.4838709677419, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=305, Percent_Identity=27.8688524590164, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=31.3636363636364, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=29.3650793650794, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=31.8965517241379, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=28, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=24.5954692556634, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24580595, Length=292, Percent_Identity=32.1917808219178, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19920448, Length=292, Percent_Identity=32.1917808219178, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24585320, Length=284, Percent_Identity=28.8732394366197, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=271, Percent_Identity=27.6752767527675, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45549423, Length=135, Percent_Identity=34.8148148148148, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37172; Mature: 37172

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSATHRARLASPLTIGSHRFASRVLQSPLAGVTDKAFRQLVRRYAPHSLLYTEMVSAS
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHC
GLHYSRSLPQIMDIAPGETPIGIQLFDCRPDFLAEAARVAEAQGADVIDINMGCPVNKIT
CCCHHCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
KNGGGSSLLRDPATAAKIVAVVAAAVSLPVTVKTRLGWSDGEINILEFARLMQESGAQML
CCCCCCCHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
TVHARTRAQSFEGTARWEWIARVKAQLAIPVIANGDIFSIDAAIRCLELTGADGVMCARG
EEEHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCEEEHHHHHEEEEECCCCCEEEECC
TMGAPHLVGQIDHYLRTGERLPDPTPRARLEMACVHLHLLFDYLGAPGVRRARKHMGWYV
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
RDFPGAAALRAQLMCIETVPEGERLLNEAIERLEAKPVLSAN
EECCCHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELSATHRARLASPLTIGSHRFASRVLQSPLAGVTDKAFRQLVRRYAPHSLLYTEMVSAS
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHC
GLHYSRSLPQIMDIAPGETPIGIQLFDCRPDFLAEAARVAEAQGADVIDINMGCPVNKIT
CCCHHCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
KNGGGSSLLRDPATAAKIVAVVAAAVSLPVTVKTRLGWSDGEINILEFARLMQESGAQML
CCCCCCCHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
TVHARTRAQSFEGTARWEWIARVKAQLAIPVIANGDIFSIDAAIRCLELTGADGVMCARG
EEEHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCEEEHHHHHEEEEECCCCCEEEECC
TMGAPHLVGQIDHYLRTGERLPDPTPRARLEMACVHLHLLFDYLGAPGVRRARKHMGWYV
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
RDFPGAAALRAQLMCIETVPEGERLLNEAIERLEAKPVLSAN
EECCCHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]