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The map label for this gene is ndhD [H]

Identifier: 37520573

GI number: 37520573

Start: 1076127

End: 1077641

Strand: Direct

Name: ndhD [H]

Synonym: gvip133

Alternate gene names: 37520573

Gene position: 1076127-1077641 (Clockwise)

Preceding gene: 37520572

Following gene: 37520574

Centisome position: 23.1

GC content: 63.7

Gene sequence:

>1515_bases
ATGTTGAGTGTGCTTATCTGGTTGCCGGTGGTGGGCGCTCTGCTGGTGGGCCTCTGGCCCGGCGCTTCCGCCGCAAGATT
GCGAAGCATCGCCCTCGCCTTCGCCACGGCCGGGGTGCTCTGGTCGGCCGGGTTGCTCTGGCAGTTCGACCCTGCCCGGC
CGGGGTTGCAGTTTGTCGAATTTATCCCCTGGATTGCCCCGGTAGGCCTGCACTACAGCCTCGGGGCGGACGGCCTCTCG
CTGCCGCTCATTGCCCTCAACAGCCTGCTCATCTGGATCGCCATCTACAGCAACGCGTACTCCGGCGAGCGCCCCAGGCT
TTACTACGGCTTGCTGTTGCTGGCCGGGGGCGGGATGGCCGGAGCGTTCGCCGCCCAGAATTTGCTGCTGTTTTTCTTGT
TCTACGAGCTGGAACTGCTGCCCTTTTATCTGCTCATCTTTTTGTGGGGCTGCAGTGAGAAGCGCGGCTACGCGGCGATG
AAATTTCTCATCTACACCGCCGTCTCGGGCTTCTTAATTCTGGGTGCTTTTCTGGGCCTAGTGCTGCTGGACGGTTCGTC
AAGCTTCGCCTACGGCGAACAATCCACCGCCGCCCTGCCGTTGATTACCCAGTTGATTCTGCTCACGATGCTGCTGGTCG
GCTTCGGCATCAAGATTCCGCTGGTGCCGCTGCACACCTGGCTGCCGGACGCCTACTGCGAAGCGTCCCCGCCGGTGGCC
ATTCTCTTAGGCGGGGTGCTCGCCAAGCTCGGAGCCTACGGCCTGCTGCGCTTCGGCCTGGGGCTCTTTCCTGAGACCTG
GGCACTGGTGGCGCCGGGGCTTGCCACGATCGGTGCAATCACCGCTGTCTACGGCGCCTTCTCCGCCATTGCCCAGAGGG
ACATCAAGCGCATGGTTGCCTACAGCTCGATTGGCCACATGGGCTATATTCTCCTGGCCGCCGCCGCCGCCACCCCTTTG
AGCCTGCTCGGGGCGATGCTGCAGATGGTGAGCCATGGCCTCATCCTCGCGATTTTGTTCCACCTGGTGGGGGTGGTCGA
AGCCAAAGTCGGCACCCGCGACCTGGACGTGCTCAACGGCCTGATGAACCCGATTCGCGGCCTGCCGATGGTGAGCGCGC
TATTGGTGCTGGGGGGGATGGCCTCCGCCGGCATTCCGGGGCTGGTGGGCTTCGTGGCCGAATTTCTGGTGTTGCAGGGC
TCCTTTGCCGCCTTCCCGGTCCAGACGATTCTGTGCGTCGTCTGCAGCGGTCTCACGGCGGTCTACTTCGTGATTTTGCT
CAACCGCACCTGCTTCGGTCGCCTGGATAACGCCACCGCCTACTACCCGCCGGTGCGCTGGATCGAGCGCACCCCTGCAT
TGATCCTCGCCGGCATCATCCTGGTCCTGGGCGTTCAGCCCGAGTGGCTCTCGCGCTGGAGCGAGGTGACCGCCGAGTCG
ATGGTTACCCCAGTTCAAAGTGCGGCCAACCAGCCCAAAATGCCGGCCGCCGCCGCTTCTCTCAGCCCGAACTAG

Upstream 100 bases:

>100_bases
ACCCAGTTCTATTTGCTCACGATCATTCTGGGGGTCGTGCTCCTCGGAATGCTGATGATTTAGCTCCTGTTCTCCACACG
ACGCGCGAGGTACGGTTGCA

Downstream 100 bases:

>100_bases
TCACCGCACGAGGTATCCGCCGATGACGACCGCCACAAAATCCCCTATCCTCCCCGGCTACAGCGGGGTGCTCCCGCCCT
CCCAGCACGAATTTGCGCAG

Product: NAD(P)H-quinone oxidoreductase subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, subunit D-2; NDH-1, chain 4-2 [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MLSVLIWLPVVGALLVGLWPGASAARLRSIALAFATAGVLWSAGLLWQFDPARPGLQFVEFIPWIAPVGLHYSLGADGLS
LPLIALNSLLIWIAIYSNAYSGERPRLYYGLLLLAGGGMAGAFAAQNLLLFFLFYELELLPFYLLIFLWGCSEKRGYAAM
KFLIYTAVSGFLILGAFLGLVLLDGSSSFAYGEQSTAALPLITQLILLTMLLVGFGIKIPLVPLHTWLPDAYCEASPPVA
ILLGGVLAKLGAYGLLRFGLGLFPETWALVAPGLATIGAITAVYGAFSAIAQRDIKRMVAYSSIGHMGYILLAAAAATPL
SLLGAMLQMVSHGLILAILFHLVGVVEAKVGTRDLDVLNGLMNPIRGLPMVSALLVLGGMASAGIPGLVGFVAEFLVLQG
SFAAFPVQTILCVVCSGLTAVYFVILLNRTCFGRLDNATAYYPPVRWIERTPALILAGIILVLGVQPEWLSRWSEVTAES
MVTPVQSAANQPKMPAAAASLSPN

Sequences:

>Translated_504_residues
MLSVLIWLPVVGALLVGLWPGASAARLRSIALAFATAGVLWSAGLLWQFDPARPGLQFVEFIPWIAPVGLHYSLGADGLS
LPLIALNSLLIWIAIYSNAYSGERPRLYYGLLLLAGGGMAGAFAAQNLLLFFLFYELELLPFYLLIFLWGCSEKRGYAAM
KFLIYTAVSGFLILGAFLGLVLLDGSSSFAYGEQSTAALPLITQLILLTMLLVGFGIKIPLVPLHTWLPDAYCEASPPVA
ILLGGVLAKLGAYGLLRFGLGLFPETWALVAPGLATIGAITAVYGAFSAIAQRDIKRMVAYSSIGHMGYILLAAAAATPL
SLLGAMLQMVSHGLILAILFHLVGVVEAKVGTRDLDVLNGLMNPIRGLPMVSALLVLGGMASAGIPGLVGFVAEFLVLQG
SFAAFPVQTILCVVCSGLTAVYFVILLNRTCFGRLDNATAYYPPVRWIERTPALILAGIILVLGVQPEWLSRWSEVTAES
MVTPVQSAANQPKMPAAAASLSPN
>Mature_504_residues
MLSVLIWLPVVGALLVGLWPGASAARLRSIALAFATAGVLWSAGLLWQFDPARPGLQFVEFIPWIAPVGLHYSLGADGLS
LPLIALNSLLIWIAIYSNAYSGERPRLYYGLLLLAGGGMAGAFAAQNLLLFFLFYELELLPFYLLIFLWGCSEKRGYAAM
KFLIYTAVSGFLILGAFLGLVLLDGSSSFAYGEQSTAALPLITQLILLTMLLVGFGIKIPLVPLHTWLPDAYCEASPPVA
ILLGGVLAKLGAYGLLRFGLGLFPETWALVAPGLATIGAITAVYGAFSAIAQRDIKRMVAYSSIGHMGYILLAAAAATPL
SLLGAMLQMVSHGLILAILFHLVGVVEAKVGTRDLDVLNGLMNPIRGLPMVSALLVLGGMASAGIPGLVGFVAEFLVLQG
SFAAFPVQTILCVVCSGLTAVYFVILLNRTCFGRLDNATAYYPPVRWIERTPALILAGIILVLGVQPEWLSRWSEVTAES
MVTPVQSAANQPKMPAAAASLSPN

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=383, Percent_Identity=27.4151436031332, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI251831117, Length=359, Percent_Identity=25.3481894150418, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788613, Length=391, Percent_Identity=38.8746803069054, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788827, Length=377, Percent_Identity=28.3819628647215, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=218, Percent_Identity=34.4036697247706, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788829, Length=423, Percent_Identity=27.1867612293144, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788614, Length=374, Percent_Identity=28.6096256684492, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367154, Length=223, Percent_Identity=31.390134529148, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 53483; Mature: 53483

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVLIWLPVVGALLVGLWPGASAARLRSIALAFATAGVLWSAGLLWQFDPARPGLQFVE
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
FIPWIAPVGLHYSLGADGLSLPLIALNSLLIWIAIYSNAYSGERPRLYYGLLLLAGGGMA
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCH
GAFAAQNLLLFFLFYELELLPFYLLIFLWGCSEKRGYAAMKFLIYTAVSGFLILGAFLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLLDGSSSFAYGEQSTAALPLITQLILLTMLLVGFGIKIPLVPLHTWLPDAYCEASPPVA
HEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCHHCCCCCCHH
ILLGGVLAKLGAYGLLRFGLGLFPETWALVAPGLATIGAITAVYGAFSAIAQRDIKRMVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIGHMGYILLAAAAATPLSLLGAMLQMVSHGLILAILFHLVGVVEAKVGTRDLDVLNG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LMNPIRGLPMVSALLVLGGMASAGIPGLVGFVAEFLVLQGSFAAFPVQTILCVVCSGLTA
HHHHHCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VYFVILLNRTCFGRLDNATAYYPPVRWIERTPALILAGIILVLGVQPEWLSRWSEVTAES
HHHHHHHHHHHHCCCCCCCEECCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVTPVQSAANQPKMPAAAASLSPN
HHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSVLIWLPVVGALLVGLWPGASAARLRSIALAFATAGVLWSAGLLWQFDPARPGLQFVE
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
FIPWIAPVGLHYSLGADGLSLPLIALNSLLIWIAIYSNAYSGERPRLYYGLLLLAGGGMA
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCH
GAFAAQNLLLFFLFYELELLPFYLLIFLWGCSEKRGYAAMKFLIYTAVSGFLILGAFLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLLDGSSSFAYGEQSTAALPLITQLILLTMLLVGFGIKIPLVPLHTWLPDAYCEASPPVA
HEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCHHCCCCCCHH
ILLGGVLAKLGAYGLLRFGLGLFPETWALVAPGLATIGAITAVYGAFSAIAQRDIKRMVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIGHMGYILLAAAAATPLSLLGAMLQMVSHGLILAILFHLVGVVEAKVGTRDLDVLNG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LMNPIRGLPMVSALLVLGGMASAGIPGLVGFVAEFLVLQGSFAAFPVQTILCVVCSGLTA
HHHHHCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VYFVILLNRTCFGRLDNATAYYPPVRWIERTPALILAGIILVLGVQPEWLSRWSEVTAES
HHHHHHHHHHHHCCCCCCCEECCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVTPVQSAANQPKMPAAAASLSPN
HHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7846157; 8905231 [H]