Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 37520572

GI number: 37520572

Start: 1074281

End: 1076089

Strand: Direct

Name: ndhF [H]

Synonym: gvip132

Alternate gene names: 37520572

Gene position: 1074281-1076089 (Clockwise)

Preceding gene: 37520569

Following gene: 37520573

Centisome position: 23.06

GC content: 63.57

Gene sequence:

>1809_bases
ATGGATCTCGCTATTGTTCAATCTGCCTGGTGGATTCCCCTGTACAATCTGGCCGGCGCCGTGCTGGCCCTGCCCTGGTC
CCCGGGGATCATCCGCAAGACCGGACCGCGCCCGGCCGGTTACCTCAATTTGATTGCGACGCTGTTTGCCCTGGTCCATT
GCCTGCTGTTGCTGCGGGAGGTCTGGGGTCAGGGGCCGGTGGCGATGAGCTTTACCTGGTTGGAGGTGCCGGGACTGACC
CTGACGCTGCCGCTGGAAATTTCGGCGGTAACGGTGACGGCGGCGGCGGTGGTGGCTGGGTTGGGCTTGCTCGCCCAGTT
GTACGCCGTCGCCTACCTGGAGATGGACTGGGGATGGGCCAGGCTCTTTTCGCTGTTGGGCCTGTTCCAGGCGGGCATGA
GCGCGCTGGTGCTGTGCAATTCGCTGTTTTTCAGCTACTTTATCCTCGAAATTCTCACCCTGGGCACCTATTTGCTGGTT
GGGTTCTGGTTCAACCAGCCCCTGGTGGTCACCGGGGCGCGCGACGCTTTTTTGACTAAGCGCGTCGGCGACCTGTTTAT
GTTGATGGGCGTACTTGCCCTCTGGCCGCTGGCGGGCACCTGGGACTTCTCGGAACTGGCGCGCTGGGCGGCTGTGACTC
CCCCGGACGCGAGTGTTGCCACCCTGTTGGGACTGGCGCTGATCGCCGGCCCGATCGGCAAGTGCGCCCAGTTCCCGCTG
CACCTGTGGCTCGACGAGGCCATGGAAGGTCCCTTGCCTTCGACCATTCTGCGCAACTCGGTGGTGGTCGCCACCGGCGC
CTGGGTGTTGGTCAAAATGTATCCGCTCGTTGCCCTCTCGCCGGTAACCGTCGCGGCCCTCATCGCCATCGGCTCGATCA
CCGCCCTGGGCGGCTCGCTGGTGGCCCTGGCCCAGATCGACGTCAAGCGGGTATTGTCGTACCTGGTGAGTGCTTACCTG
GGCCTGGTGCTCGTCGCAGTGGCCACCGGCCAGCTTTCGACCGCGCTGCTGCTGCTTTTGGCCAATGCCCTGGCGATGGC
GCTGTTGGTGATGGCCGTGGGTTCGGCAGTCTGGAACAACATCAGCCAGGATCTCAGCCAGTTGGGCGGTCTGTGGTCGC
GTCGGCCCGTCACCGCGCTGTGCTATCTGACCGGCTGCTTTGGGTTGGTGGCGGCTTTGCCCCTCGGGGGCCTTTGGGCG
CAACTGGAGATGGTCGAGGGGCTCTGGAACGCCCAGCCGCTTTTGGCTGTGCTCGTGGTGGTCGTCAATGCCCTGGCCGG
TTTTGCTTTGGGCCGCGTCTTCGGTCTGATTTTCCTGGGCAAGCCTACCCAGATGACCGTGCGCTCGCCTGAGGCGCCCT
GGCCGATGGTGGTGCCGATGACGGTGCTCGCCGGTTTGGTGATTCATTTTCCCATCGTGCTTGCCACGATCGGCTTGCTG
CCCGGCACCGCCGCCAATCCGCTGGCGCTCCCCGCCCTGGTGGCCCTAGTGGTGCCGGGCCTGGCAGGCGTGGTGCTCGG
AGCGCGGCTCAGGCCGGTGGAATTGCCCAACCGCCCGGTGCAGGACTGGCTGGCCAACGACTTTTACACCGCCGTCGCCT
ACAAGAACACCATCGTCTTTGTGATCGGTTTGATTTCGCAGTTCGTTTCACTCTTTGACCGCTACGTGGTCGACGGGTTG
GTGAACCTGGTGGGGCAGCTCTCGTTTTTGGGTGGCGAGGGCATCAAGTACAGCACCACCGGCCAGACCCAGTTCTATTT
GCTCACGATCATTCTGGGGGTCGTGCTCCTCGGAATGCTGATGATTTAG

Upstream 100 bases:

>100_bases
GCAATAGTCCAAGCGGGTCGAAAAACGGTCCCTCGCGCCGCGGGGAGCCGCTCGGGCCGGATGTTCGTGCATGTGTCCAG
CGTCGGGAGTTCTGGCTGCG

Downstream 100 bases:

>100_bases
CTCCTGTTCTCCACACGACGCGCGAGGTACGGTTGCAATGTTGAGTGTGCTTATCTGGTTGCCGGTGGTGGGCGCTCTGC
TGGTGGGCCTCTGGCCCGGC

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MDLAIVQSAWWIPLYNLAGAVLALPWSPGIIRKTGPRPAGYLNLIATLFALVHCLLLLREVWGQGPVAMSFTWLEVPGLT
LTLPLEISAVTVTAAAVVAGLGLLAQLYAVAYLEMDWGWARLFSLLGLFQAGMSALVLCNSLFFSYFILEILTLGTYLLV
GFWFNQPLVVTGARDAFLTKRVGDLFMLMGVLALWPLAGTWDFSELARWAAVTPPDASVATLLGLALIAGPIGKCAQFPL
HLWLDEAMEGPLPSTILRNSVVVATGAWVLVKMYPLVALSPVTVAALIAIGSITALGGSLVALAQIDVKRVLSYLVSAYL
GLVLVAVATGQLSTALLLLLANALAMALLVMAVGSAVWNNISQDLSQLGGLWSRRPVTALCYLTGCFGLVAALPLGGLWA
QLEMVEGLWNAQPLLAVLVVVVNALAGFALGRVFGLIFLGKPTQMTVRSPEAPWPMVVPMTVLAGLVIHFPIVLATIGLL
PGTAANPLALPALVALVVPGLAGVVLGARLRPVELPNRPVQDWLANDFYTAVAYKNTIVFVIGLISQFVSLFDRYVVDGL
VNLVGQLSFLGGEGIKYSTTGQTQFYLLTIILGVVLLGMLMI

Sequences:

>Translated_602_residues
MDLAIVQSAWWIPLYNLAGAVLALPWSPGIIRKTGPRPAGYLNLIATLFALVHCLLLLREVWGQGPVAMSFTWLEVPGLT
LTLPLEISAVTVTAAAVVAGLGLLAQLYAVAYLEMDWGWARLFSLLGLFQAGMSALVLCNSLFFSYFILEILTLGTYLLV
GFWFNQPLVVTGARDAFLTKRVGDLFMLMGVLALWPLAGTWDFSELARWAAVTPPDASVATLLGLALIAGPIGKCAQFPL
HLWLDEAMEGPLPSTILRNSVVVATGAWVLVKMYPLVALSPVTVAALIAIGSITALGGSLVALAQIDVKRVLSYLVSAYL
GLVLVAVATGQLSTALLLLLANALAMALLVMAVGSAVWNNISQDLSQLGGLWSRRPVTALCYLTGCFGLVAALPLGGLWA
QLEMVEGLWNAQPLLAVLVVVVNALAGFALGRVFGLIFLGKPTQMTVRSPEAPWPMVVPMTVLAGLVIHFPIVLATIGLL
PGTAANPLALPALVALVVPGLAGVVLGARLRPVELPNRPVQDWLANDFYTAVAYKNTIVFVIGLISQFVSLFDRYVVDGL
VNLVGQLSFLGGEGIKYSTTGQTQFYLLTIILGVVLLGMLMI
>Mature_602_residues
MDLAIVQSAWWIPLYNLAGAVLALPWSPGIIRKTGPRPAGYLNLIATLFALVHCLLLLREVWGQGPVAMSFTWLEVPGLT
LTLPLEISAVTVTAAAVVAGLGLLAQLYAVAYLEMDWGWARLFSLLGLFQAGMSALVLCNSLFFSYFILEILTLGTYLLV
GFWFNQPLVVTGARDAFLTKRVGDLFMLMGVLALWPLAGTWDFSELARWAAVTPPDASVATLLGLALIAGPIGKCAQFPL
HLWLDEAMEGPLPSTILRNSVVVATGAWVLVKMYPLVALSPVTVAALIAIGSITALGGSLVALAQIDVKRVLSYLVSAYL
GLVLVAVATGQLSTALLLLLANALAMALLVMAVGSAVWNNISQDLSQLGGLWSRRPVTALCYLTGCFGLVAALPLGGLWA
QLEMVEGLWNAQPLLAVLVVVVNALAGFALGRVFGLIFLGKPTQMTVRSPEAPWPMVVPMTVLAGLVIHFPIVLATIGLL
PGTAANPLALPALVALVVPGLAGVVLGARLRPVELPNRPVQDWLANDFYTAVAYKNTIVFVIGLISQFVSLFDRYVVDGL
VNLVGQLSFLGGEGIKYSTTGQTQFYLLTIILGVVLLGMLMI

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=304, Percent_Identity=29.2763157894737, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788614, Length=547, Percent_Identity=28.5191956124314, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788827, Length=400, Percent_Identity=27.5, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788613, Length=315, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788829, Length=473, Percent_Identity=24.3128964059197, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788831, Length=407, Percent_Identity=25.3071253071253, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367154, Length=350, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 63958; Mature: 63958

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAIVQSAWWIPLYNLAGAVLALPWSPGIIRKTGPRPAGYLNLIATLFALVHCLLLLRE
CCCCEECCCCCHHHHHHCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VWGQGPVAMSFTWLEVPGLTLTLPLEISAVTVTAAAVVAGLGLLAQLYAVAYLEMDWGWA
HHCCCCEEEEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RLFSLLGLFQAGMSALVLCNSLFFSYFILEILTLGTYLLVGFWFNQPLVVTGARDAFLTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
RVGDLFMLMGVLALWPLAGTWDFSELARWAAVTPPDASVATLLGLALIAGPIGKCAQFPL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
HLWLDEAMEGPLPSTILRNSVVVATGAWVLVKMYPLVALSPVTVAALIAIGSITALGGSL
HHHHHHHHCCCCCHHHHHCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHH
VALAQIDVKRVLSYLVSAYLGLVLVAVATGQLSTALLLLLANALAMALLVMAVGSAVWNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISQDLSQLGGLWSRRPVTALCYLTGCFGLVAALPLGGLWAQLEMVEGLWNAQPLLAVLVV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
VVNALAGFALGRVFGLIFLGKPTQMTVRSPEAPWPMVVPMTVLAGLVIHFPIVLATIGLL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCC
PGTAANPLALPALVALVVPGLAGVVLGARLRPVELPNRPVQDWLANDFYTAVAYKNTIVF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VIGLISQFVSLFDRYVVDGLVNLVGQLSFLGGEGIKYSTTGQTQFYLLTIILGVVLLGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
MI
HC
>Mature Secondary Structure
MDLAIVQSAWWIPLYNLAGAVLALPWSPGIIRKTGPRPAGYLNLIATLFALVHCLLLLRE
CCCCEECCCCCHHHHHHCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VWGQGPVAMSFTWLEVPGLTLTLPLEISAVTVTAAAVVAGLGLLAQLYAVAYLEMDWGWA
HHCCCCEEEEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RLFSLLGLFQAGMSALVLCNSLFFSYFILEILTLGTYLLVGFWFNQPLVVTGARDAFLTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
RVGDLFMLMGVLALWPLAGTWDFSELARWAAVTPPDASVATLLGLALIAGPIGKCAQFPL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
HLWLDEAMEGPLPSTILRNSVVVATGAWVLVKMYPLVALSPVTVAALIAIGSITALGGSL
HHHHHHHHCCCCCHHHHHCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHH
VALAQIDVKRVLSYLVSAYLGLVLVAVATGQLSTALLLLLANALAMALLVMAVGSAVWNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISQDLSQLGGLWSRRPVTALCYLTGCFGLVAALPLGGLWAQLEMVEGLWNAQPLLAVLVV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
VVNALAGFALGRVFGLIFLGKPTQMTVRSPEAPWPMVVPMTVLAGLVIHFPIVLATIGLL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCC
PGTAANPLALPALVALVVPGLAGVVLGARLRPVELPNRPVQDWLANDFYTAVAYKNTIVF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VIGLISQFVSLFDRYVVDGLVNLVGQLSFLGGEGIKYSTTGQTQFYLLTIILGVVLLGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
MI
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]