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The map label for this gene is yqiI [H]

Identifier: 37520534

GI number: 37520534

Start: 1025320

End: 1027053

Strand: Direct

Name: yqiI [H]

Synonym: glr0965

Alternate gene names: 37520534

Gene position: 1025320-1027053 (Clockwise)

Preceding gene: 37520530

Following gene: 37520535

Centisome position: 22.01

GC content: 66.78

Gene sequence:

>1734_bases
GTGAGGAACAAACGGGTTGTACTGTGGTCTGCAAGCAGCGTGCTCGCCTGGGCGTCCTGTCTTGTCTGCTGCTCCACCGC
CCTGGCGATGCCGCAACTGACCGGCTGGCAATTCGACTCCCAGGCTCAGCAGCTTTCATTTTTTACCCGCGGCAGCGTGC
AGCCGCGCATTCAGATTGTCGAAAAACCCCGACGGGTTGTTGTCGATTTGCCGGGGGCCGATGTGTTCGCACCCTCGGAC
GCGCCGGTGAGCAGCGGTCCGGTGCGCTTCGTGCGCGCCGGGCAATTCGACCCGCAGACGGCGCGCATGGTGATGGAACT
GGCCGAGGATGGCCCGCAGCTGCAGCCTGAGCAGGTGCGCGTGCGCCAGGTGGCCCCCGACCAATGGCTGGTGCAACTGC
TGCCGAACGTGCCGCCGCCGCCAAGCGCCCCGGCCGCTCCACCGGTGGTCGCGCCGCCGCTGACGATGCCGCCTGCGGTC
GGTCGCCGGGTCCTGGTAGGCGGGATCGAGGTGCGCCCCGAAGGATTTTTGGTCAAAACGGGCGGCTACGTGCGCTCCGA
CGCCCGGCTGCTGGAGGAGCAACCGCCGCGCGTCGTCGTCGACATCGAAGAGGCCGAACTGGCCAAGAATTTTGCCGAGC
GCAATCTGGCCGTCAACCAGCAGGGCGTCACCCGCCTACGCACCGGTCAATTCCAGGACGACCCGCCGCTGGTGAGGGTG
GTGCTCGATCTGGGGGCAGGGGCGGCCAATGCCTGGGAAGCGCGCTACAGCGCCGATTTGGGCGGGGTGCTCATCCGCCC
GGCCGGTTCCGCGCCCGCCGCCACGGCTCCCACCGGCGAAAAAGTGTCCTTGCAATCGGCCCAATTGACTCCTGAAGGCC
TGGTGTTTAACAGCGACCTGCCGCCGCGGCTGGAGACCAACTGGGAGAATCCCAACGAATTTCGGATTGTCTTCAGCCCC
GCGCAGCTACCGGCCAACTTCTCCGGACCGCTCATCGACGCGGCAAGCCCCATCGACAACCTCAACATCCGCCAGGTCGA
CGATCGCACGGTGGTGGCACTGGTGCGGGTGTTACCCGGCACGCGGGTGGGCGATCCGAGGCCGCTCGACGGCGAACGGC
GCCGGGTGCTGGTGCCTCTGTACCGGCGCAGCACCACACCGACGACACCCGTTCCCGATAGCTATGACCCGCTTCCCAAC
GGCAGCGGCCGGCGCATCGTCCTCGACGCCGGGCACGGCGGCAAAGACCCCGGCGCCATGCGCGAGGGGGTGCGCGAAAA
AGATCTCAACCTGGCCATCGTCCGCCGCCTCAACAACAAGCTGCGCGCCGCCGGCTACTACACGATCCTGGCGCGCTCCG
ACGACACGTTCATCTCGCTGGGTGAGCGCGTCGACATCACCAAGGCGACCCAGGGCGACATCTTCGTGAGCGTCCACGTC
AACACGATGCCCAGCCGCAGCGATATCCAGGGCATCGAGACTTACTATACTCACTCCCGAAGCGCCCGCCTCGCCTACGT
GCTGCACCGCCGTCTGGTCGAGCGCACCGGCAAGCCCGATCGCGGCGTGCGGGTGCGCGGTCTCTATGTAACCCGCCACA
ACGCCGTGCCTGCGGTGTTGCTGGAGGTGGGGTTTTTGACCAATCCCGAGGAGCGCGCCCAGTTGCAGCAGCCTGAGTAT
CAGGAACTGATCGCCGACGCCATTGCCCAGGGATTGCAGGACTACGCCCGTTAG

Upstream 100 bases:

>100_bases
AAGGAGGGTTTATTAAGTGGTTGGTGCCGAAAAATACGCTCGATCGCAACAGAAAACGCACCAAAGGTTGCTATAGTAAT
TGAAAATTTTGGCACGGCGT

Downstream 100 bases:

>100_bases
GCCGGACCGGTCGATGACTAGACCGCTTGCGCGAATGCCAAGATAAACAGCTCAAACCATTGGAAGCTCATTCCGCAATC
CGGTTCATGCAAGAGCTCTA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MRNKRVVLWSASSVLAWASCLVCCSTALAMPQLTGWQFDSQAQQLSFFTRGSVQPRIQIVEKPRRVVVDLPGADVFAPSD
APVSSGPVRFVRAGQFDPQTARMVMELAEDGPQLQPEQVRVRQVAPDQWLVQLLPNVPPPPSAPAAPPVVAPPLTMPPAV
GRRVLVGGIEVRPEGFLVKTGGYVRSDARLLEEQPPRVVVDIEEAELAKNFAERNLAVNQQGVTRLRTGQFQDDPPLVRV
VLDLGAGAANAWEARYSADLGGVLIRPAGSAPAATAPTGEKVSLQSAQLTPEGLVFNSDLPPRLETNWENPNEFRIVFSP
AQLPANFSGPLIDAASPIDNLNIRQVDDRTVVALVRVLPGTRVGDPRPLDGERRRVLVPLYRRSTTPTTPVPDSYDPLPN
GSGRRIVLDAGHGGKDPGAMREGVREKDLNLAIVRRLNNKLRAAGYYTILARSDDTFISLGERVDITKATQGDIFVSVHV
NTMPSRSDIQGIETYYTHSRSARLAYVLHRRLVERTGKPDRGVRVRGLYVTRHNAVPAVLLEVGFLTNPEERAQLQQPEY
QELIADAIAQGLQDYAR

Sequences:

>Translated_577_residues
MRNKRVVLWSASSVLAWASCLVCCSTALAMPQLTGWQFDSQAQQLSFFTRGSVQPRIQIVEKPRRVVVDLPGADVFAPSD
APVSSGPVRFVRAGQFDPQTARMVMELAEDGPQLQPEQVRVRQVAPDQWLVQLLPNVPPPPSAPAAPPVVAPPLTMPPAV
GRRVLVGGIEVRPEGFLVKTGGYVRSDARLLEEQPPRVVVDIEEAELAKNFAERNLAVNQQGVTRLRTGQFQDDPPLVRV
VLDLGAGAANAWEARYSADLGGVLIRPAGSAPAATAPTGEKVSLQSAQLTPEGLVFNSDLPPRLETNWENPNEFRIVFSP
AQLPANFSGPLIDAASPIDNLNIRQVDDRTVVALVRVLPGTRVGDPRPLDGERRRVLVPLYRRSTTPTTPVPDSYDPLPN
GSGRRIVLDAGHGGKDPGAMREGVREKDLNLAIVRRLNNKLRAAGYYTILARSDDTFISLGERVDITKATQGDIFVSVHV
NTMPSRSDIQGIETYYTHSRSARLAYVLHRRLVERTGKPDRGVRVRGLYVTRHNAVPAVLLEVGFLTNPEERAQLQQPEY
QELIADAIAQGLQDYAR
>Mature_577_residues
MRNKRVVLWSASSVLAWASCLVCCSTALAMPQLTGWQFDSQAQQLSFFTRGSVQPRIQIVEKPRRVVVDLPGADVFAPSD
APVSSGPVRFVRAGQFDPQTARMVMELAEDGPQLQPEQVRVRQVAPDQWLVQLLPNVPPPPSAPAAPPVVAPPLTMPPAV
GRRVLVGGIEVRPEGFLVKTGGYVRSDARLLEEQPPRVVVDIEEAELAKNFAERNLAVNQQGVTRLRTGQFQDDPPLVRV
VLDLGAGAANAWEARYSADLGGVLIRPAGSAPAATAPTGEKVSLQSAQLTPEGLVFNSDLPPRLETNWENPNEFRIVFSP
AQLPANFSGPLIDAASPIDNLNIRQVDDRTVVALVRVLPGTRVGDPRPLDGERRRVLVPLYRRSTTPTTPVPDSYDPLPN
GSGRRIVLDAGHGGKDPGAMREGVREKDLNLAIVRRLNNKLRAAGYYTILARSDDTFISLGERVDITKATQGDIFVSVHV
NTMPSRSDIQGIETYYTHSRSARLAYVLHRRLVERTGKPDRGVRVRGLYVTRHNAVPAVLLEVGFLTNPEERAQLQQPEY
QELIADAIAQGLQDYAR

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=229, Percent_Identity=29.6943231441048, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=27.2340425531915, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790611, Length=84, Percent_Identity=40.4761904761905, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 63142; Mature: 63142

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKRVVLWSASSVLAWASCLVCCSTALAMPQLTGWQFDSQAQQLSFFTRGSVQPRIQIV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHEE
EKPRRVVVDLPGADVFAPSDAPVSSGPVRFVRAGQFDPQTARMVMELAEDGPQLQPEQVR
ECCCEEEEECCCCCEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHH
VRQVAPDQWLVQLLPNVPPPPSAPAAPPVVAPPLTMPPAVGRRVLVGGIEVRPEGFLVKT
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECEEECCCCEEEEC
GGYVRSDARLLEEQPPRVVVDIEEAELAKNFAERNLAVNQQGVTRLRTGQFQDDPPLVRV
CCEECHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCEECCCCCHHHCCCCCCCCCCCEEE
VLDLGAGAANAWEARYSADLGGVLIRPAGSAPAATAPTGEKVSLQSAQLTPEGLVFNSDL
EEHHCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCC
PPRLETNWENPNEFRIVFSPAQLPANFSGPLIDAASPIDNLNIRQVDDRTVVALVRVLPG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCEEEECCHHHHHHHHHCCC
TRVGDPRPLDGERRRVLVPLYRRSTTPTTPVPDSYDPLPNGSGRRIVLDAGHGGKDPGAM
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
REGVREKDLNLAIVRRLNNKLRAAGYYTILARSDDTFISLGERVDITKATQGDIFVSVHV
HHCCHHCCCCHHHHHHHHHHHEECEEEEEEEECCCCEEECCCEEEEEECCCCCEEEEEEE
NTMPSRSDIQGIETYYTHSRSARLAYVLHRRLVERTGKPDRGVRVRGLYVTRHNAVPAVL
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEEE
LEVGFLTNPEERAQLQQPEYQELIADAIAQGLQDYAR
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNKRVVLWSASSVLAWASCLVCCSTALAMPQLTGWQFDSQAQQLSFFTRGSVQPRIQIV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHEE
EKPRRVVVDLPGADVFAPSDAPVSSGPVRFVRAGQFDPQTARMVMELAEDGPQLQPEQVR
ECCCEEEEECCCCCEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHH
VRQVAPDQWLVQLLPNVPPPPSAPAAPPVVAPPLTMPPAVGRRVLVGGIEVRPEGFLVKT
HHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECEEECCCCEEEEC
GGYVRSDARLLEEQPPRVVVDIEEAELAKNFAERNLAVNQQGVTRLRTGQFQDDPPLVRV
CCEECHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCEECCCCCHHHCCCCCCCCCCCEEE
VLDLGAGAANAWEARYSADLGGVLIRPAGSAPAATAPTGEKVSLQSAQLTPEGLVFNSDL
EEHHCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCC
PPRLETNWENPNEFRIVFSPAQLPANFSGPLIDAASPIDNLNIRQVDDRTVVALVRVLPG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCEEEECCHHHHHHHHHCCC
TRVGDPRPLDGERRRVLVPLYRRSTTPTTPVPDSYDPLPNGSGRRIVLDAGHGGKDPGAM
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
REGVREKDLNLAIVRRLNNKLRAAGYYTILARSDDTFISLGERVDITKATQGDIFVSVHV
HHCCHHCCCCHHHHHHHHHHHEECEEEEEEEECCCCEEECCCEEEEEECCCCCEEEEEEE
NTMPSRSDIQGIETYYTHSRSARLAYVLHRRLVERTGKPDRGVRVRGLYVTRHNAVPAVL
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEEE
LEVGFLTNPEERAQLQQPEYQELIADAIAQGLQDYAR
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]