Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 37520490

GI number: 37520490

Start: 977830

End: 979407

Strand: Reverse

Name: epsE [H]

Synonym: gll0921

Alternate gene names: 37520490

Gene position: 979407-977830 (Counterclockwise)

Preceding gene: 37520493

Following gene: 37520489

Centisome position: 21.02

GC content: 63.69

Gene sequence:

>1578_bases
ATGCAAGGTTATACGCTGCCCCCGGAATTGAAACTGCCCATCGCCGAAAGCCCCGGTATCGGCGTCGTGCTGCTTCCCGA
GTCGCTCGATTGGAACTTGATCCGCTCGCTGAGCGGCAAGCTCGGCGAAGAACTGCGCTCTATCGTCCCGCTCGCCTTTT
TTAACAAACAGCTTTATCTGGGTAGCTTCAGCGCCCTCGGCGAGACCGATCTGCGCCGATTGCGCGAGAAACTGCCCTGC
GAGCTTCGCGCCCTGACCCTGGCTTCCTCAGAGGTGCGCCGCTGGTGGCAAATCGGGCGCGAGCGGCTGCCGGAATATTT
TCAGGCTGCCGGAGGGGGCGGTCTTGAGCCCCTCGCGGCGGCCGAACCCTCGGTTGCCATCACCATCGGCACCGACGGCA
CTCCCGAAGGCAAGCGCATCAGCGCCCAGTTGCAAAGACTCCTCAACGAAGCGCTCAAAAGCCGCGCAAGCGACATCCAC
CTCGAATCGCAGGAAGACGGTCTCAGGGTGCGCTTTCGCATCGACGGCCTGCTGCGCGAGATGGGCACTTTTCCGGTTGC
CGAGAGCAAAGCGCTCATCTCACGGGTCAAAGTGCTCGCCGAACTCGACATCGCCAACCACAGAAGCCCCCAGGACGGCC
GCGTCACCCAAGAAATCAACGGCCAACTAGTCGATTTGCGCGTGAGCACCCTGCCCTGCCTGCACGGCGAAAAAGCAGTC
CTGCGGCTGCTGCCCAAGAACAACACCTTCACGCTGCTGGAGGAACTAGGATTTGACGCGGCGGACCTGGCCACCTATCG
CCACTGGCTCGGCCGCGCCCAGGGGTTAATCCTCATCACCGGCCCCACCGGCTCCGGCAAGACCAGCACACTCTATACCA
GCCTGCGCGGGATCCTCAAGCCCGAGTGCAACATCGTCACGATCGAAGATCCGATCGAATACCAGCTTGCCGGGATCAAC
CAGGTGCAGGTGCACCCGAAGGCGGGTCTCACCTTCGCAAGCGGCCTGCGCTCGATTCTCCGGCAAGACCCCGACATCAT
CATGGTGGGTGAAATCCGCGACCTGGAGACCGCCCAGACGGTGTTCCAGGCGGCGATGACCGGTCACCTGGTGCTGTGCA
CACTGCACACCAACGACGCGCCCAGTGCCGTGAGCCGCCTCCTGGACATGCAGGTGGAGCCTTATCTCATCGCCGGGGCG
CTGGTGGGGGTGGTTGCCCAGCGCCTCGTGCGCCGAGTGTGCCGCCACTGCGGCGAACCGTACTGCCCCGACAGCGTCGA
TCTGCAGCGGCTCAAACTGGACGCCCACCCCGAGTACGCCAATGTTCCGCTCGAATGGAAGCGCGCCCACGGTTGCCAGC
ACTGCTTCGGGTCGGGCTACTTCGGCCGCGAGGGCATCTTCGAGGTGATGGAAGTCGACAACCACCTGCAGGGGCTCATC
CACGCCCGCGCCTCCAACGCCGAAATCACCACCTACCTGCGCGCGCGCAACATCCGCTCGCTGCCCGAGGCCGGCATCGC
CAAGGTCATGGAGGGCAGCACCACCATTGAGGAACTTCTGCGGGTTGTGTCAGTCTAG

Upstream 100 bases:

>100_bases
CCAAGCGGTGATAATGTGCGGATGGTTGCAATGATCACTTTTCAGGAACCCTGAAGTACCTCTGGATCGTCCAACTGCCT
AGATTAAAAAGTGGGTACCT

Downstream 100 bases:

>100_bases
ACGGGCGTCCATCGGAGAAAAATGCACGTGAAACGCTGGCTGAGCTTGCCCGCCGGATTCCTGCTTGCCGGCTGTCTGAG
TACGGCGGCCTGGGCCGCCC

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MQGYTLPPELKLPIAESPGIGVVLLPESLDWNLIRSLSGKLGEELRSIVPLAFFNKQLYLGSFSALGETDLRRLREKLPC
ELRALTLASSEVRRWWQIGRERLPEYFQAAGGGGLEPLAAAEPSVAITIGTDGTPEGKRISAQLQRLLNEALKSRASDIH
LESQEDGLRVRFRIDGLLREMGTFPVAESKALISRVKVLAELDIANHRSPQDGRVTQEINGQLVDLRVSTLPCLHGEKAV
LRLLPKNNTFTLLEELGFDAADLATYRHWLGRAQGLILITGPTGSGKTSTLYTSLRGILKPECNIVTIEDPIEYQLAGIN
QVQVHPKAGLTFASGLRSILRQDPDIIMVGEIRDLETAQTVFQAAMTGHLVLCTLHTNDAPSAVSRLLDMQVEPYLIAGA
LVGVVAQRLVRRVCRHCGEPYCPDSVDLQRLKLDAHPEYANVPLEWKRAHGCQHCFGSGYFGREGIFEVMEVDNHLQGLI
HARASNAEITTYLRARNIRSLPEAGIAKVMEGSTTIEELLRVVSV

Sequences:

>Translated_525_residues
MQGYTLPPELKLPIAESPGIGVVLLPESLDWNLIRSLSGKLGEELRSIVPLAFFNKQLYLGSFSALGETDLRRLREKLPC
ELRALTLASSEVRRWWQIGRERLPEYFQAAGGGGLEPLAAAEPSVAITIGTDGTPEGKRISAQLQRLLNEALKSRASDIH
LESQEDGLRVRFRIDGLLREMGTFPVAESKALISRVKVLAELDIANHRSPQDGRVTQEINGQLVDLRVSTLPCLHGEKAV
LRLLPKNNTFTLLEELGFDAADLATYRHWLGRAQGLILITGPTGSGKTSTLYTSLRGILKPECNIVTIEDPIEYQLAGIN
QVQVHPKAGLTFASGLRSILRQDPDIIMVGEIRDLETAQTVFQAAMTGHLVLCTLHTNDAPSAVSRLLDMQVEPYLIAGA
LVGVVAQRLVRRVCRHCGEPYCPDSVDLQRLKLDAHPEYANVPLEWKRAHGCQHCFGSGYFGREGIFEVMEVDNHLQGLI
HARASNAEITTYLRARNIRSLPEAGIAKVMEGSTTIEELLRVVSV
>Mature_525_residues
MQGYTLPPELKLPIAESPGIGVVLLPESLDWNLIRSLSGKLGEELRSIVPLAFFNKQLYLGSFSALGETDLRRLREKLPC
ELRALTLASSEVRRWWQIGRERLPEYFQAAGGGGLEPLAAAEPSVAITIGTDGTPEGKRISAQLQRLLNEALKSRASDIH
LESQEDGLRVRFRIDGLLREMGTFPVAESKALISRVKVLAELDIANHRSPQDGRVTQEINGQLVDLRVSTLPCLHGEKAV
LRLLPKNNTFTLLEELGFDAADLATYRHWLGRAQGLILITGPTGSGKTSTLYTSLRGILKPECNIVTIEDPIEYQLAGIN
QVQVHPKAGLTFASGLRSILRQDPDIIMVGEIRDLETAQTVFQAAMTGHLVLCTLHTNDAPSAVSRLLDMQVEPYLIAGA
LVGVVAQRLVRRVCRHCGEPYCPDSVDLQRLKLDAHPEYANVPLEWKRAHGCQHCFGSGYFGREGIFEVMEVDNHLQGLI
HARASNAEITTYLRARNIRSLPEAGIAKVMEGSTTIEELLRVVSV

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=378, Percent_Identity=47.0899470899471, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1786296, Length=387, Percent_Identity=45.7364341085271, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI87082188, Length=286, Percent_Identity=36.7132867132867, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57840; Mature: 57840

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGYTLPPELKLPIAESPGIGVVLLPESLDWNLIRSLSGKLGEELRSIVPLAFFNKQLYL
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GSFSALGETDLRRLREKLPCELRALTLASSEVRRWWQIGRERLPEYFQAAGGGGLEPLAA
CCCHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AEPSVAITIGTDGTPEGKRISAQLQRLLNEALKSRASDIHLESQEDGLRVRFRIDGLLRE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCEEEEEEHHHHHH
MGTFPVAESKALISRVKVLAELDIANHRSPQDGRVTQEINGQLVDLRVSTLPCLHGEKAV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCHH
LRLLPKNNTFTLLEELGFDAADLATYRHWLGRAQGLILITGPTGSGKTSTLYTSLRGILK
EEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
PECNIVTIEDPIEYQLAGINQVQVHPKAGLTFASGLRSILRQDPDIIMVGEIRDLETAQT
CCCCEEEECCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
VFQAAMTGHLVLCTLHTNDAPSAVSRLLDMQVEPYLIAGALVGVVAQRLVRRVCRHCGEP
HHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YCPDSVDLQRLKLDAHPEYANVPLEWKRAHGCQHCFGSGYFGREGIFEVMEVDNHLQGLI
CCCCCCCHHHEEECCCCCCCCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HARASNAEITTYLRARNIRSLPEAGIAKVMEGSTTIEELLRVVSV
HHCCCCCCEEHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQGYTLPPELKLPIAESPGIGVVLLPESLDWNLIRSLSGKLGEELRSIVPLAFFNKQLYL
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GSFSALGETDLRRLREKLPCELRALTLASSEVRRWWQIGRERLPEYFQAAGGGGLEPLAA
CCCHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AEPSVAITIGTDGTPEGKRISAQLQRLLNEALKSRASDIHLESQEDGLRVRFRIDGLLRE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCEEEEEEHHHHHH
MGTFPVAESKALISRVKVLAELDIANHRSPQDGRVTQEINGQLVDLRVSTLPCLHGEKAV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCHH
LRLLPKNNTFTLLEELGFDAADLATYRHWLGRAQGLILITGPTGSGKTSTLYTSLRGILK
EEEECCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
PECNIVTIEDPIEYQLAGINQVQVHPKAGLTFASGLRSILRQDPDIIMVGEIRDLETAQT
CCCCEEEECCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
VFQAAMTGHLVLCTLHTNDAPSAVSRLLDMQVEPYLIAGALVGVVAQRLVRRVCRHCGEP
HHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YCPDSVDLQRLKLDAHPEYANVPLEWKRAHGCQHCFGSGYFGREGIFEVMEVDNHLQGLI
CCCCCCCHHHEEECCCCCCCCCCCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HARASNAEITTYLRARNIRSLPEAGIAKVMEGSTTIEELLRVVSV
HHCCCCCCEEHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]