Click here to switch to the map view.

The map label for this gene is pycB [H]

Identifier: 37520448

GI number: 37520448

Start: 929709

End: 931235

Strand: Reverse

Name: pycB [H]

Synonym: gll0879

Alternate gene names: 37520448

Gene position: 931235-929709 (Counterclockwise)

Preceding gene: 37520449

Following gene: 37520447

Centisome position: 19.99

GC content: 63.79

Gene sequence:

>1527_bases
TTGACCTCGATTGCGGTTGATCCCCACAGCGAGTGGCAACTATCGACCGGGTCCACTTCCCGGGTCGGCGTCACCGATAC
CACCTTTCGCGACGCCCACCAATCGCTTTTGGCCACGCGCATGACCACGGCCCAGATGTTGCCGGTCGCGTCGCGGATGA
ACCGCATCGGTTTTCATTCGATGGAGGTCTGGGGCGGCGCCACCTTCGACGCCTGTATCCGGTTTTTGGGCGAAGATCCG
TGGGAGCGGCTGAAAGTTTTGCGCCGCGAGATGCCCGACACCCGCCTGCAGATGCTGGTGCGCGGACAGAACCTGCTCGG
TTACCGCCATTATCCTGACGACATCGTCGAGCGATTCATTCGCCGCTCGGTGGCCAACGGCATCGATATCATCCGCACCT
TCGACGCCCTCAACGACGTGCGCAACATGCGCAAAACGATCGAGACGGGCAAGGAGTGCGGTGCCCACGTGCAGGGCACG
CTGGTCTATACCGTGAGCCCCGTGCACGACGTTGATCATTACATGGCGGTTGCCCGTGACCTGGTAGCTATGGGTATCGA
CTCGTTGTGCATCAAAGATATGGCGGGTCTGCTGAAACCCGACGCCGCCGGCCGCCTCGTGCGCGCTCTGCGCCCGGTGG
TGGGTACCCTGCTGCCTATCCAGATGCACGCCCATTCGCTCTCGGGCATGGCCTCGATGGCGTACTGGGAAGCCATCCAG
ATGGGAGCCAACGCCGTCGACACGGCCATTTCGCCTCTGGCCCTGGGATCGAGCCAGCCGGCCACCGAGACGATGGTAGT
GGTACTGCGCGATACGCCCTTCGACACGGGCCTCGACATTGACGCTTTAGTCGACGTGGCCGAATCTATCGAGCGCATCT
TCCGCGACGCGGGCAACGCCCCGATTCGCCAGACGATCATCGACACGCGCGTACTCTCGCACCAGGTGCCCGGCGGCATG
ATCACCAACTTGCTCGCCCAACTCGACCAGCAGCGCGCTTCGCATCGCCTGCAAGAAGTGCTGCGCGAATTGCCGCGGGT
GCGCGCCGATTTTGGCTATCCGCCCCTGGTCACCCCCACCAGCCAGATCGTGGGCACCCAGGCGGTGCTCAACGTGCTGA
TGGGCGAGCGCTACAAGATTATCCCGGCCGAGGCGCGCGACTATCTCTTGGGGCGCTACGGCCGCCCGCCCCACCCCATC
GCCGAGCATCTGTTCGAGCGCGTCGCCGAAGGCCGGCAACCGATCACCCACCGACCCGCCGACGATCTCGAGCCGGGTTG
GGAGCCGGCGCTGGCCGAAAGTGCCCCCTGGGCCACTTCTGAGGAAGATGTGCTTACCTATGCGATGTTTCCGCAGGTGG
CCACCAACTACTTCAAAGCCCGGGCGCGCAAGCTTTGCGGCGAGCGTCGGCCCTTCCCGGTCGCACTGCGTCAGGTGAAC
GAAATGGTGCGCGGTTTTGCCGAAGCGACTGCTCTCGATCGCTTTGAGTGCGAAGTGAACGGTCTGCGCCTTCGACTGGT
CCGCTAA

Upstream 100 bases:

>100_bases
GTCCGGCTCCAAGGTGGATATGGTCGTCGGTGCCGTTCCGAAGACGACGCTGGCCACCACCCTCGAAAAGCATATCGATA
TCAAATAGTGCAGGTGTGCT

Downstream 100 bases:

>100_bases
TTGCCGGTGATTGCCGGCATTTATCGCTATCGTTAGTTTCGGTACAGCTTGGGAAAACCCACTGCGTTGAGTTGTCGTCC
AAACTCAAGCAAGCCCGACG

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTSIAVDPHSEWQLSTGSTSRVGVTDTTFRDAHQSLLATRMTTAQMLPVASRMNRIGFHSMEVWGGATFDACIRFLGEDP
WERLKVLRREMPDTRLQMLVRGQNLLGYRHYPDDIVERFIRRSVANGIDIIRTFDALNDVRNMRKTIETGKECGAHVQGT
LVYTVSPVHDVDHYMAVARDLVAMGIDSLCIKDMAGLLKPDAAGRLVRALRPVVGTLLPIQMHAHSLSGMASMAYWEAIQ
MGANAVDTAISPLALGSSQPATETMVVVLRDTPFDTGLDIDALVDVAESIERIFRDAGNAPIRQTIIDTRVLSHQVPGGM
ITNLLAQLDQQRASHRLQEVLRELPRVRADFGYPPLVTPTSQIVGTQAVLNVLMGERYKIIPAEARDYLLGRYGRPPHPI
AEHLFERVAEGRQPITHRPADDLEPGWEPALAESAPWATSEEDVLTYAMFPQVATNYFKARARKLCGERRPFPVALRQVN
EMVRGFAEATALDRFECEVNGLRLRLVR

Sequences:

>Translated_508_residues
MTSIAVDPHSEWQLSTGSTSRVGVTDTTFRDAHQSLLATRMTTAQMLPVASRMNRIGFHSMEVWGGATFDACIRFLGEDP
WERLKVLRREMPDTRLQMLVRGQNLLGYRHYPDDIVERFIRRSVANGIDIIRTFDALNDVRNMRKTIETGKECGAHVQGT
LVYTVSPVHDVDHYMAVARDLVAMGIDSLCIKDMAGLLKPDAAGRLVRALRPVVGTLLPIQMHAHSLSGMASMAYWEAIQ
MGANAVDTAISPLALGSSQPATETMVVVLRDTPFDTGLDIDALVDVAESIERIFRDAGNAPIRQTIIDTRVLSHQVPGGM
ITNLLAQLDQQRASHRLQEVLRELPRVRADFGYPPLVTPTSQIVGTQAVLNVLMGERYKIIPAEARDYLLGRYGRPPHPI
AEHLFERVAEGRQPITHRPADDLEPGWEPALAESAPWATSEEDVLTYAMFPQVATNYFKARARKLCGERRPFPVALRQVN
EMVRGFAEATALDRFECEVNGLRLRLVR
>Mature_507_residues
TSIAVDPHSEWQLSTGSTSRVGVTDTTFRDAHQSLLATRMTTAQMLPVASRMNRIGFHSMEVWGGATFDACIRFLGEDPW
ERLKVLRREMPDTRLQMLVRGQNLLGYRHYPDDIVERFIRRSVANGIDIIRTFDALNDVRNMRKTIETGKECGAHVQGTL
VYTVSPVHDVDHYMAVARDLVAMGIDSLCIKDMAGLLKPDAAGRLVRALRPVVGTLLPIQMHAHSLSGMASMAYWEAIQM
GANAVDTAISPLALGSSQPATETMVVVLRDTPFDTGLDIDALVDVAESIERIFRDAGNAPIRQTIIDTRVLSHQVPGGMI
TNLLAQLDQQRASHRLQEVLRELPRVRADFGYPPLVTPTSQIVGTQAVLNVLMGERYKIIPAEARDYLLGRYGRPPHPIA
EHLFERVAEGRQPITHRPADDLEPGWEPALAESAPWATSEEDVLTYAMFPQVATNYFKARARKLCGERRPFPVALRQVNE
MVRGFAEATALDRFECEVNGLRLRLVR

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=455, Percent_Identity=35.1648351648352, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI106049295, Length=455, Percent_Identity=35.1648351648352, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI106049292, Length=455, Percent_Identity=35.1648351648352, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17562816, Length=460, Percent_Identity=36.304347826087, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319695, Length=461, Percent_Identity=34.2733188720174, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321376, Length=461, Percent_Identity=34.056399132321, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI281363050, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652224, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652222, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652220, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652218, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652212, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652210, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652214, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921944, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24652216, Length=463, Percent_Identity=34.7732181425486, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 56647; Mature: 56516

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIAVDPHSEWQLSTGSTSRVGVTDTTFRDAHQSLLATRMTTAQMLPVASRMNRIGFHS
CCCEECCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
MEVWGGATFDACIRFLGEDPWERLKVLRREMPDTRLQMLVRGQNLLGYRHYPDDIVERFI
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCCCCHHHHHHHH
RRSVANGIDIIRTFDALNDVRNMRKTIETGKECGAHVQGTLVYTVSPVHDVDHYMAVARD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHH
LVAMGIDSLCIKDMAGLLKPDAAGRLVRALRPVVGTLLPIQMHAHSLSGMASMAYWEAIQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGANAVDTAISPLALGSSQPATETMVVVLRDTPFDTGLDIDALVDVAESIERIFRDAGNA
HCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PIRQTIIDTRVLSHQVPGGMITNLLAQLDQQRASHRLQEVLRELPRVRADFGYPPLVTPT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
SQIVGTQAVLNVLMGERYKIIPAEARDYLLGRYGRPPHPIAEHLFERVAEGRQPITHRPA
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DDLEPGWEPALAESAPWATSEEDVLTYAMFPQVATNYFKARARKLCGERRPFPVALRQVN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EMVRGFAEATALDRFECEVNGLRLRLVR
HHHHHHHHHHHHHHHEEEECCEEEEEEC
>Mature Secondary Structure 
TSIAVDPHSEWQLSTGSTSRVGVTDTTFRDAHQSLLATRMTTAQMLPVASRMNRIGFHS
CCEECCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
MEVWGGATFDACIRFLGEDPWERLKVLRREMPDTRLQMLVRGQNLLGYRHYPDDIVERFI
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCCCCHHHHHHHH
RRSVANGIDIIRTFDALNDVRNMRKTIETGKECGAHVQGTLVYTVSPVHDVDHYMAVARD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHH
LVAMGIDSLCIKDMAGLLKPDAAGRLVRALRPVVGTLLPIQMHAHSLSGMASMAYWEAIQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGANAVDTAISPLALGSSQPATETMVVVLRDTPFDTGLDIDALVDVAESIERIFRDAGNA
HCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PIRQTIIDTRVLSHQVPGGMITNLLAQLDQQRASHRLQEVLRELPRVRADFGYPPLVTPT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
SQIVGTQAVLNVLMGERYKIIPAEARDYLLGRYGRPPHPIAEHLFERVAEGRQPITHRPA
HHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DDLEPGWEPALAESAPWATSEEDVLTYAMFPQVATNYFKARARKLCGERRPFPVALRQVN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EMVRGFAEATALDRFECEVNGLRLRLVR
HHHHHHHHHHHHHHHEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]